Polimorfismo Pro12Ala no gene PPARG como fator de risco para morbidades associadas ao envelhecimento

• Autor(a) principal: Festi, Regiane Rodrigues
• Data de Publicação: 2015
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFMT
• Texto Completo: http://ri.ufmt.br/handle/1/702
• topic: Envelhecimento; Fatores de risco; Morbidades; Polimorfismo 5; PPARG; CNPQ::CIENCIAS DA SAUDE; Aging; Risk factors; Morbidity; Polymorphism

PPARgamma is considered a ligand dependent transcription factor. When active is responsible for adipocyte differentiation, and helps to control mitochondrial biogenesis and participates in the regulation of amyloidogenic pathways, reducing the amount of beta-amyloid protein, often found in senile plaques in patients diagnosed with Alzheimer's disease. Polymorphisms of the PPARG may be found associated with major morbidity related to aging and the Pro12Ala polymorphism. Objective: This study aimed to estimate the allele and genotype frequency of polymorphism to assess whether the presence of Pro12Ala could be associated with the presence morbidities in this population. Methods: We conducted a study of the type descriptive cross-sectional. The sample was composed of 544 individuals of both sexes, over 49 years old. All participants underwent standardized interview, laboratory tests and validated tests as Katz, Pfeffer, Mini Mental State Examination, Clock Test, Verbal Fluency, abbreviated scale Yesavage Fangestrom, Gage. Peripheral blood was collected from each participant and DNA samples extracted by salting out technique and genotyped by PCR-RFLP technique for the identification of the Pro12Ala polymorphism (rs1801282). Results: The genotype frequency for Pro / Pro was 75.4% for Pro / Ala was 22% and Ala / Ala was 2.6%. The frequency of the C allele was 86% and the G allele, 14%. There was not a statistically significant result when comparing the presence of polymorphisms with ethnicity and gender. When we analyzed if the presence of polymorphisms was associated with metabolic diseases, degenerative diseases and dementia, we found no statistically significant result. We found a statistically significant result when comparing the genotypes with the clock drawing test (p = 0.022) and verbal fluency test (p = 0.046), where Ala allele carriers had higher test scores than individuals with the wild-type allele (Pro / Pro ), showing that the polymorphic allele may be acting as a protective factor for these individuals. For the Katz test, we found a statistically significant result (p = 0.034), carrying the polymorphic allele are more independent than those with the wild type allele. The linear regression model showed a significant result for the Clock Test, Verbal Fluency and Katz when related to genotype and age. Conclusion: We found no polymorphism association with metabolic diseases, degenerative dementia, with gender or ethnicity. However it was observed that individuals carrying the allele 12Ala have greater independence and cognitive preservation, thus showing that the polymorphism may be acting as a protective factor for these individuals when it comes the Clock Test, Verbal Fluency and Katz.