Efeitos antioxidante, anti-inflamatório e metabólico da botriosferana em animais obesos e não obesos com o tumor de Walker-256

Detalhes bibliográficos
Autor(a) principal: Silva, Thais Pereira da
Data de Publicação: 2021
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFMT
Texto Completo: http://ri.ufmt.br/handle/1/4856
Resumo: Botryosphaeran, a (1-3)(1-6)-β-D-glucan, has been described for presenting several activities, such as antimutagenic, antiproliferative, hypoglycemic, and hypocholesterolemic activity, besides demonstrating an important antitumoral effect in obese and non-obese animals with Walker-256 tumor.Thus, this study aimed to evaluate the effects of botryosphaeran on morphological changes in the tumor, liver, spleen, and small intestine in obese and non-obese animals with Walker-256 tumor, as well as to evaluate the presence of metastasis, metabolic and inflammatory parameters and the oxidative stress in these animals. Wistar rats, approximately 30 days old, were used. The animals were divided into 4 groups: Control Tumor (CT); Control Tumor Botryosphaeran (CTB); Obese Tumor (OT), and Obese Tumor Botryosphaeran (OTB). Control animals received standard diet and obese animals received hyperlipidic diet and water with sucrose. On 9th week, 1x107 Walker-256 tumor cells were inoculated in all rats of all groups, and the CTB and OTB groups received treatment with botryosphaeran (30 mg/kg b.w./day, for 15 days, from the 9th to the 11th week of experiment) and controls (CT and OT) received saline. At the end of the 11th week of the experiment, all groups were evaluated. Oxidative stress markers (TBARS; carbonylated proteins; superoxide dismutase (SOD); catalase (CAT); glutathione S transferase (GST); reduced glutathione (GSH); and vitamin C), metabolic parameters (glucose, glycogen, lactate, total protein and amino acids), cytokines (IL-1β; IL-6; IL-10 and TNF-α) and histological evaluation of tissues (spleen, liver, small intestine and tumor) were evaluated. Obesity contributed to increased TBARS levels in the OT group compared to the CT group, and increased SOD activity and TNF-α levels in liver tissue. Obesity reduced CAT, GSH, glucose, and IL-10 levels in the OT group relative to the CT group. Botryosphaeran decreased liver TBARS, lactate and TNF-α levels and TBARS and lactate levels in adipose tissue of obese rats, increased hepatic glucose, IL-10 levels, and GSH levels and GST activity in liver, as increased hepatic vitamin C levels. In tumor tissue, botryosphaeran increased SOD activity in CTB animals and vitamin C levels in OTB animals. It was also observed that all groups presented areas of coagulative tumor necrosis, and botryosphaeran was effective in reducing the area of necrosis in the CTB and OTB groups. In the liver, inflammation and hepatic steatosis were observed in all groups (CT, CTB, OT, and OTB), with hepatic steatosis being more frequent and more intense in the obese group (OT). Botryosphaeran reduced the number of animals with hepatic steatosis in the OTB group. Finally, botryosphaeran reduced the area of necrosis in the tumor tissue of CTB and OTB animals. Botryosphaeran showed a protective effect against vascular invasion in tumor tissue in CTB and OTB groups. Thus, it can be concluded that obesity contributed to oxidative stress, metabolic alterations, and inflammatory mediators, and botryosphaeran was effective in protecting the animals against tissue injury triggered by oxidative stress, due to its significant antioxidant, anti-inflammatory and beneficial metabolic effects in obese and non-obese animals with Walker-256 tumor.
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spelling Efeitos antioxidante, anti-inflamatório e metabólico da botriosferana em animais obesos e não obesos com o tumor de Walker-256ObesidadeCâncerβ-glucanasBotriosferanaEstresse oxidativoCNPQ::CIENCIAS DA SAUDEObesityCancerβ-glucansBotryosphaeranOxidative stressBotryosphaeran, a (1-3)(1-6)-β-D-glucan, has been described for presenting several activities, such as antimutagenic, antiproliferative, hypoglycemic, and hypocholesterolemic activity, besides demonstrating an important antitumoral effect in obese and non-obese animals with Walker-256 tumor.Thus, this study aimed to evaluate the effects of botryosphaeran on morphological changes in the tumor, liver, spleen, and small intestine in obese and non-obese animals with Walker-256 tumor, as well as to evaluate the presence of metastasis, metabolic and inflammatory parameters and the oxidative stress in these animals. Wistar rats, approximately 30 days old, were used. The animals were divided into 4 groups: Control Tumor (CT); Control Tumor Botryosphaeran (CTB); Obese Tumor (OT), and Obese Tumor Botryosphaeran (OTB). Control animals received standard diet and obese animals received hyperlipidic diet and water with sucrose. On 9th week, 1x107 Walker-256 tumor cells were inoculated in all rats of all groups, and the CTB and OTB groups received treatment with botryosphaeran (30 mg/kg b.w./day, for 15 days, from the 9th to the 11th week of experiment) and controls (CT and OT) received saline. At the end of the 11th week of the experiment, all groups were evaluated. Oxidative stress markers (TBARS; carbonylated proteins; superoxide dismutase (SOD); catalase (CAT); glutathione S transferase (GST); reduced glutathione (GSH); and vitamin C), metabolic parameters (glucose, glycogen, lactate, total protein and amino acids), cytokines (IL-1β; IL-6; IL-10 and TNF-α) and histological evaluation of tissues (spleen, liver, small intestine and tumor) were evaluated. Obesity contributed to increased TBARS levels in the OT group compared to the CT group, and increased SOD activity and TNF-α levels in liver tissue. Obesity reduced CAT, GSH, glucose, and IL-10 levels in the OT group relative to the CT group. Botryosphaeran decreased liver TBARS, lactate and TNF-α levels and TBARS and lactate levels in adipose tissue of obese rats, increased hepatic glucose, IL-10 levels, and GSH levels and GST activity in liver, as increased hepatic vitamin C levels. In tumor tissue, botryosphaeran increased SOD activity in CTB animals and vitamin C levels in OTB animals. It was also observed that all groups presented areas of coagulative tumor necrosis, and botryosphaeran was effective in reducing the area of necrosis in the CTB and OTB groups. In the liver, inflammation and hepatic steatosis were observed in all groups (CT, CTB, OT, and OTB), with hepatic steatosis being more frequent and more intense in the obese group (OT). Botryosphaeran reduced the number of animals with hepatic steatosis in the OTB group. Finally, botryosphaeran reduced the area of necrosis in the tumor tissue of CTB and OTB animals. Botryosphaeran showed a protective effect against vascular invasion in tumor tissue in CTB and OTB groups. Thus, it can be concluded that obesity contributed to oxidative stress, metabolic alterations, and inflammatory mediators, and botryosphaeran was effective in protecting the animals against tissue injury triggered by oxidative stress, due to its significant antioxidant, anti-inflammatory and beneficial metabolic effects in obese and non-obese animals with Walker-256 tumor.Botriosferana, uma β-(1-3)(1-6)-D-glucana, tem sido descrita por apresentar atividade antimutagênica, antiproliferativa, hipoglicemiante e hipocolesterolêmica além de demonstrar importante efeito antitumoral em animais obesos e não obesos com tumor de Walker-256. Assim, o objetivo deste trabalho foi avaliar os efeitos da botriosferana sobre as alterações morfológicas do tumor, do fígado, do baço e do intestino delgado em animais obesos e não obesos com o tumor de Walker-256, bem como avaliar parâmetros metabólicos, inflamatórios e o estresse oxidativo nestes animais. Foram utilizados ratos Wistar, com aproximadamente 30 dias de idade. Os animais foram divididos em 4 grupos: Controle Tumor (CT); Controle Tumor Botriosferana (CTB); Obeso Tumor (OT) e Obeso Tumor Botriosferana (OTB). Os animais controles receberam ração padrão para roedores e os obesos receberam ração hiperlipídica e água com sacarose. Na 9a semana, foram inoculadas 1x107 células de tumor de Walker-256 via intraperitoneal, subcutâneo do flanco direito e os grupos CTB e OTB receberam botriosferana (30 mg/kg, via gavagem, durante 15 dias, da 9ª à 11ª semana de experimento) e os controles (CT e OT) receberam solução salina. No final da 11a semana todos os grupos foram avaliados. Foram avaliados os marcadores de estresse oxidativo (TBARS; proteínas carboniladas; superóxido dismutase (SOD); catalase (CAT); glutationa S transferase (GST); glutationa reduzida (GSH); e vitamina C), parâmetros metabólicos (glicose, o glicogênio, lactato, proteínas totais e aminoácidos), citocinas (IL-1β; IL-6; IL-10 e TNF-α) e avaliação histológica dos tecidos (baço, fígado, intestino delgado e tumor). A obesidade contribuiu para o aumento dos níveis de TBARS no grupo OT em relação ao grupo CT, e aumentou a atividade da SOD e os níveis de TNF-α no tecido hepático. A obesidade reduziu os níveis de CAT, GSH, glicose e IL-10 no grupo OT em relação ao grupo CT. A botriosferana diminuiu os níveis de TBARS, lactato e TNF-α hepático e os níveis de TBARS e lactato no tecido adiposo dos ratos obesos, aumentou a glicose hepática, os níveis de IL-10 e GSH e a atividade da GST no fígado, bem como aumentou os níveis de vitamina C hepático. No tecido tumoral, a botriosferana aumentou a atividade da SOD nos animais CTB e os níveis de vitamina C nos animais OTB. Ainda, observou-se que todos os grupos apresentaram áreas de necrose tumoral coagulativa, sendo que a botriosferana foi eficaz na redução da área de necrose no grupo CTB e OTB. No fígado, observou-se presença de processo inflamatório e esteatose hepática em todos os grupos (CT, CTB, OT e OTB), sendo a esteatose hepática mais frequente e mais intensa no grupo obeso (OT). A botriosferana reduziu o número de animais com esteatose hepática no grupo OTB. Por fim, a botriosferana reduziu a área de necrose no tecido tumoral dos animais CTB e OTB. A botriosferana apresentou efeito protetor contra invasão vascular no tecido tumoral nos grupos CTB e OTB. Assim, pode-se concluir, a partir dos resultados observados que a obesidade, de fato, contribuiu para o aumento do estresse oxidativo, alterações metabólicas e mediadores inflamatórios e a botriosferana foi eficaz em proteger os animais contra lesões teciduais desencadeadas pelo estresse oxidativo, devido ao seu significante efeito antioxidante, antiinflamatório, metabólicos em animais obesos e não obesos com o tumor de Walker-256.Universidade Federal de Mato GrossoBrasilInstituto de Ciências da Saúde (ICS) - SinopUFMT CUS - SinopPrograma de Pós-Graduação em Ciências em SaúdeQueiroz, Eveline Aparecida Isquierdo Fonseca deSinhorin, Valeria Dornelles Gindrihttp://lattes.cnpq.br/3818211604255549http://lattes.cnpq.br/6880858782538903Queiroz, Eveline Aparecida Isquierdo Fonseca de042.780.249-08http://lattes.cnpq.br/6880858782538903Dias, Marcos Correa303.669.798-50http://lattes.cnpq.br/6197853669797333042.780.249-08890.945.900-00Vessoni, Sandra Coccuzzo Sampaio255.543.458-59http://lattes.cnpq.br/6181892645004033Silva, Thais Pereira da2023-11-23T12:40:16Z2021-07-202023-11-23T12:40:16Z2021-05-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisSILVA, Thais Pereira da. Efeitos antioxidante, anti-inflamatório e metabólico da botriosferana em animais obesos e não obesos com o tumor de Walker-256. 2021. 134 f. Dissertação (Mestrado em Ciências em Saúde) - Universidade Federal de Mato Grosso, Campus Universitário de Sinop, Instituto de Ciências da Saúde, Sinop, 2021.http://ri.ufmt.br/handle/1/4856porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMTinstname:Universidade Federal de Mato Grosso (UFMT)instacron:UFMT2023-11-25T06:01:51Zoai:localhost:1/4856Repositório InstitucionalPUBhttp://ri.ufmt.br/oai/requestjordanbiblio@gmail.comopendoar:2023-11-25T06:01:51Repositório Institucional da UFMT - Universidade Federal de Mato Grosso (UFMT)false
dc.title.none.fl_str_mv Efeitos antioxidante, anti-inflamatório e metabólico da botriosferana em animais obesos e não obesos com o tumor de Walker-256
title Efeitos antioxidante, anti-inflamatório e metabólico da botriosferana em animais obesos e não obesos com o tumor de Walker-256
spellingShingle Efeitos antioxidante, anti-inflamatório e metabólico da botriosferana em animais obesos e não obesos com o tumor de Walker-256
Silva, Thais Pereira da
Obesidade
Câncer
β-glucanas
Botriosferana
Estresse oxidativo
CNPQ::CIENCIAS DA SAUDE
Obesity
Cancer
β-glucans
Botryosphaeran
Oxidative stress
title_short Efeitos antioxidante, anti-inflamatório e metabólico da botriosferana em animais obesos e não obesos com o tumor de Walker-256
title_full Efeitos antioxidante, anti-inflamatório e metabólico da botriosferana em animais obesos e não obesos com o tumor de Walker-256
title_fullStr Efeitos antioxidante, anti-inflamatório e metabólico da botriosferana em animais obesos e não obesos com o tumor de Walker-256
title_full_unstemmed Efeitos antioxidante, anti-inflamatório e metabólico da botriosferana em animais obesos e não obesos com o tumor de Walker-256
title_sort Efeitos antioxidante, anti-inflamatório e metabólico da botriosferana em animais obesos e não obesos com o tumor de Walker-256
author Silva, Thais Pereira da
author_facet Silva, Thais Pereira da
author_role author
dc.contributor.none.fl_str_mv Queiroz, Eveline Aparecida Isquierdo Fonseca de
Sinhorin, Valeria Dornelles Gindri
http://lattes.cnpq.br/3818211604255549
http://lattes.cnpq.br/6880858782538903
Queiroz, Eveline Aparecida Isquierdo Fonseca de
042.780.249-08
http://lattes.cnpq.br/6880858782538903
Dias, Marcos Correa
303.669.798-50
http://lattes.cnpq.br/6197853669797333
042.780.249-08
890.945.900-00
Vessoni, Sandra Coccuzzo Sampaio
255.543.458-59
http://lattes.cnpq.br/6181892645004033
dc.contributor.author.fl_str_mv Silva, Thais Pereira da
dc.subject.por.fl_str_mv Obesidade
Câncer
β-glucanas
Botriosferana
Estresse oxidativo
CNPQ::CIENCIAS DA SAUDE
Obesity
Cancer
β-glucans
Botryosphaeran
Oxidative stress
topic Obesidade
Câncer
β-glucanas
Botriosferana
Estresse oxidativo
CNPQ::CIENCIAS DA SAUDE
Obesity
Cancer
β-glucans
Botryosphaeran
Oxidative stress
description Botryosphaeran, a (1-3)(1-6)-β-D-glucan, has been described for presenting several activities, such as antimutagenic, antiproliferative, hypoglycemic, and hypocholesterolemic activity, besides demonstrating an important antitumoral effect in obese and non-obese animals with Walker-256 tumor.Thus, this study aimed to evaluate the effects of botryosphaeran on morphological changes in the tumor, liver, spleen, and small intestine in obese and non-obese animals with Walker-256 tumor, as well as to evaluate the presence of metastasis, metabolic and inflammatory parameters and the oxidative stress in these animals. Wistar rats, approximately 30 days old, were used. The animals were divided into 4 groups: Control Tumor (CT); Control Tumor Botryosphaeran (CTB); Obese Tumor (OT), and Obese Tumor Botryosphaeran (OTB). Control animals received standard diet and obese animals received hyperlipidic diet and water with sucrose. On 9th week, 1x107 Walker-256 tumor cells were inoculated in all rats of all groups, and the CTB and OTB groups received treatment with botryosphaeran (30 mg/kg b.w./day, for 15 days, from the 9th to the 11th week of experiment) and controls (CT and OT) received saline. At the end of the 11th week of the experiment, all groups were evaluated. Oxidative stress markers (TBARS; carbonylated proteins; superoxide dismutase (SOD); catalase (CAT); glutathione S transferase (GST); reduced glutathione (GSH); and vitamin C), metabolic parameters (glucose, glycogen, lactate, total protein and amino acids), cytokines (IL-1β; IL-6; IL-10 and TNF-α) and histological evaluation of tissues (spleen, liver, small intestine and tumor) were evaluated. Obesity contributed to increased TBARS levels in the OT group compared to the CT group, and increased SOD activity and TNF-α levels in liver tissue. Obesity reduced CAT, GSH, glucose, and IL-10 levels in the OT group relative to the CT group. Botryosphaeran decreased liver TBARS, lactate and TNF-α levels and TBARS and lactate levels in adipose tissue of obese rats, increased hepatic glucose, IL-10 levels, and GSH levels and GST activity in liver, as increased hepatic vitamin C levels. In tumor tissue, botryosphaeran increased SOD activity in CTB animals and vitamin C levels in OTB animals. It was also observed that all groups presented areas of coagulative tumor necrosis, and botryosphaeran was effective in reducing the area of necrosis in the CTB and OTB groups. In the liver, inflammation and hepatic steatosis were observed in all groups (CT, CTB, OT, and OTB), with hepatic steatosis being more frequent and more intense in the obese group (OT). Botryosphaeran reduced the number of animals with hepatic steatosis in the OTB group. Finally, botryosphaeran reduced the area of necrosis in the tumor tissue of CTB and OTB animals. Botryosphaeran showed a protective effect against vascular invasion in tumor tissue in CTB and OTB groups. Thus, it can be concluded that obesity contributed to oxidative stress, metabolic alterations, and inflammatory mediators, and botryosphaeran was effective in protecting the animals against tissue injury triggered by oxidative stress, due to its significant antioxidant, anti-inflammatory and beneficial metabolic effects in obese and non-obese animals with Walker-256 tumor.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-20
2021-05-18
2023-11-23T12:40:16Z
2023-11-23T12:40:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv SILVA, Thais Pereira da. Efeitos antioxidante, anti-inflamatório e metabólico da botriosferana em animais obesos e não obesos com o tumor de Walker-256. 2021. 134 f. Dissertação (Mestrado em Ciências em Saúde) - Universidade Federal de Mato Grosso, Campus Universitário de Sinop, Instituto de Ciências da Saúde, Sinop, 2021.
http://ri.ufmt.br/handle/1/4856
identifier_str_mv SILVA, Thais Pereira da. Efeitos antioxidante, anti-inflamatório e metabólico da botriosferana em animais obesos e não obesos com o tumor de Walker-256. 2021. 134 f. Dissertação (Mestrado em Ciências em Saúde) - Universidade Federal de Mato Grosso, Campus Universitário de Sinop, Instituto de Ciências da Saúde, Sinop, 2021.
url http://ri.ufmt.br/handle/1/4856
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Mato Grosso
Brasil
Instituto de Ciências da Saúde (ICS) - Sinop
UFMT CUS - Sinop
Programa de Pós-Graduação em Ciências em Saúde
publisher.none.fl_str_mv Universidade Federal de Mato Grosso
Brasil
Instituto de Ciências da Saúde (ICS) - Sinop
UFMT CUS - Sinop
Programa de Pós-Graduação em Ciências em Saúde
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMT
instname:Universidade Federal de Mato Grosso (UFMT)
instacron:UFMT
instname_str Universidade Federal de Mato Grosso (UFMT)
instacron_str UFMT
institution UFMT
reponame_str Repositório Institucional da UFMT
collection Repositório Institucional da UFMT
repository.name.fl_str_mv Repositório Institucional da UFMT - Universidade Federal de Mato Grosso (UFMT)
repository.mail.fl_str_mv jordanbiblio@gmail.com
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