Incubação com pentoxifilina do espermatozoide bovino capacitado
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFMT |
Texto Completo: | http://ri.ufmt.br/handle/1/2507 |
Resumo: | The aim of the present study was to evaluate the effect of different concentrations of pentoxifylline on the viability, quality and oxidative stress in post-thawed and in vitro capacitated bovine sperm. Cryopreserved samples of nine Nellore bulls (Bos taurus indicus) were submitted to selection and sperm capacitation using a 45% and 90% Percoll® gradient. Samples were ressuspended in fertilization medium, and assigned in three treatments: control (miliQ water), 2.5 mM pentoxifylline and 5.0 mM pentoxifylline. Then, the samples were incubated in a water bath at 37 ° C for 3 minutes. After incubation, sperm motility and vigor, plasma membrane viability, mitochondrial cytochemical activity, acrosomal membrane integrity and oxidative stress were assessed. The data were analyzed by ANOVA, Wilcoxon and Tukey test. Significance level was set as 5% using SAS System version 9.2 software. Oxidative stress was higher in 2.5 mM treatment compared to the control and 5.0 mM pentoxifylline treatments (0.42 ± 0.06 0.28 ± 0.04 and 0.31 ± 0.04 ng / mL TBARS, respectively). A higher percentage of sperm with no mitochondrial cytochemical activity (DABIV) in 5.0 mM treatment (10.32 ± 1.39%) was was noticed in comparison to 2.5 mM treatment (5.89 ± 1.95%) and control (5.67 ± 1.17%). No differences were observed among treatments for the remaining variables. Therefore, the addition of pentoxifylline at 2.5mM and 5mM was not efficient to improve the sperm parameters of thawed bovine sperm, capacitated and submitted to in vitro incubation. |
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Incubação com pentoxifilina do espermatozoide bovino capacitadoMetilxantinaSêmenNeloreEstresse oxidativoCNPQ::CIENCIAS AGRARIASMethylxanthineSemenNelloreOxidative stressThe aim of the present study was to evaluate the effect of different concentrations of pentoxifylline on the viability, quality and oxidative stress in post-thawed and in vitro capacitated bovine sperm. Cryopreserved samples of nine Nellore bulls (Bos taurus indicus) were submitted to selection and sperm capacitation using a 45% and 90% Percoll® gradient. Samples were ressuspended in fertilization medium, and assigned in three treatments: control (miliQ water), 2.5 mM pentoxifylline and 5.0 mM pentoxifylline. Then, the samples were incubated in a water bath at 37 ° C for 3 minutes. After incubation, sperm motility and vigor, plasma membrane viability, mitochondrial cytochemical activity, acrosomal membrane integrity and oxidative stress were assessed. The data were analyzed by ANOVA, Wilcoxon and Tukey test. Significance level was set as 5% using SAS System version 9.2 software. Oxidative stress was higher in 2.5 mM treatment compared to the control and 5.0 mM pentoxifylline treatments (0.42 ± 0.06 0.28 ± 0.04 and 0.31 ± 0.04 ng / mL TBARS, respectively). A higher percentage of sperm with no mitochondrial cytochemical activity (DABIV) in 5.0 mM treatment (10.32 ± 1.39%) was was noticed in comparison to 2.5 mM treatment (5.89 ± 1.95%) and control (5.67 ± 1.17%). No differences were observed among treatments for the remaining variables. Therefore, the addition of pentoxifylline at 2.5mM and 5mM was not efficient to improve the sperm parameters of thawed bovine sperm, capacitated and submitted to in vitro incubation.CNPqO objetivo deste trabalho foi avaliar o efeito da incubação com diferentes concentrações de pentoxifilina sobre a viabilidade, qualidade e estresse oxidativo de espermatozoides bovinos descongelados e capacitados in vitro. Foram utilizadas amostras criopreservadas de nove touros da raça Nelore (Bos taurus indicus), submetidas à seleção e capacitação espermática, através de gradiente descontínuo de Percoll® 45% e 90%. Estas foram ressuspendidas em meio de fecundação e posteriormente sub-divididas em três tratamentos: controle (água miliQ), 2,5 mM de pentoxifilina e 5,0 mM de pentoxifilina. Em seguida, as amostras foram incubadas em banho-maria à 37°C por três minutos. Após a incubação, foram realizadas análises de motilidade e vigor espermáticos, viabilidade da membrana plasmática, atividade citoquímica mitocondrial, integridade de membrana acrossomal e estresse oxidativo. O dados foram analisados nos testes ANOVA, Wilcoxon e Tukey, considerando o nível de significância de 5%, utilizando o software SAS System versão 9.2. O estresse oxidativo foi superior no tratamento 2,5 mM em relação aos tratamentos controle e 5,0 mM de pentoxifilina (0,42±0,06 0,28±0,04 e 0,31±0,04 ng/mL de TBARS, respectivamente). Foi observado maior número de espermatozoides com ausência de atividade citoquímica mitocondrial (DABIV) no tratamento 5,0 mM (10,32±1,39%) em relação aos tratamentos 2,5 mM (5,89±1,95%) e controle (5,67±1,17%). Para as demais variáveis estudadas não foram observadas diferenças entre os tratamentos. Sendo assim, constatou-se que adição de pentoxifilina nas concentrações estudadas, não foi eficiente na melhoria dos parâmetros espermáticos de espermatozoides bovinos descongelados, capacitados e submetidos à incubação in vitro.Universidade Federal de Mato GrossoBrasilFaculdade de Agronomia, Medicina Veterinária e Zootecnia (FAMEVZ)UFMT CUC - CuiabáPrograma de Pós-Graduação em Ciência AnimalZervoudakis, Luciana Keiko HatamotoMotheo, Tathiana Fergusonhttp://lattes.cnpq.br/2674926039341361http://lattes.cnpq.br/2386300229256235Zervoudakis, Luciana Keiko Hatamoto186.706.438-39http://lattes.cnpq.br/2386300229256235Motheo, Tathiana Ferguson220.775.098-13http://lattes.cnpq.br/2674926039341361186.706.438-39220.775.098-13Melo Oña, Cely Marini801.799.751-53http://lattes.cnpq.br/4530682105818830Sousa, Alessandra de Moraes2021-05-13T19:42:35Z2018-07-202021-05-13T19:42:35Z2018-05-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisSOUSA, Alessandra de Moraes. Incubação com pentoxifilina do espermatozoide bovino capacitado. 2018. 46 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Mato Grosso, Faculdade de Agronomia, Medicina Veterinária e Zootecnia, Cuiabá, 2018.http://ri.ufmt.br/handle/1/2507porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMTinstname:Universidade Federal de Mato Grosso (UFMT)instacron:UFMT2021-05-15T07:01:55Zoai:localhost:1/2507Repositório InstitucionalPUBhttp://ri.ufmt.br/oai/requestjordanbiblio@gmail.comopendoar:2021-05-15T07:01:55Repositório Institucional da UFMT - Universidade Federal de Mato Grosso (UFMT)false |
dc.title.none.fl_str_mv |
Incubação com pentoxifilina do espermatozoide bovino capacitado |
title |
Incubação com pentoxifilina do espermatozoide bovino capacitado |
spellingShingle |
Incubação com pentoxifilina do espermatozoide bovino capacitado Sousa, Alessandra de Moraes Metilxantina Sêmen Nelore Estresse oxidativo CNPQ::CIENCIAS AGRARIAS Methylxanthine Semen Nellore Oxidative stress |
title_short |
Incubação com pentoxifilina do espermatozoide bovino capacitado |
title_full |
Incubação com pentoxifilina do espermatozoide bovino capacitado |
title_fullStr |
Incubação com pentoxifilina do espermatozoide bovino capacitado |
title_full_unstemmed |
Incubação com pentoxifilina do espermatozoide bovino capacitado |
title_sort |
Incubação com pentoxifilina do espermatozoide bovino capacitado |
author |
Sousa, Alessandra de Moraes |
author_facet |
Sousa, Alessandra de Moraes |
author_role |
author |
dc.contributor.none.fl_str_mv |
Zervoudakis, Luciana Keiko Hatamoto Motheo, Tathiana Ferguson http://lattes.cnpq.br/2674926039341361 http://lattes.cnpq.br/2386300229256235 Zervoudakis, Luciana Keiko Hatamoto 186.706.438-39 http://lattes.cnpq.br/2386300229256235 Motheo, Tathiana Ferguson 220.775.098-13 http://lattes.cnpq.br/2674926039341361 186.706.438-39 220.775.098-13 Melo Oña, Cely Marini 801.799.751-53 http://lattes.cnpq.br/4530682105818830 |
dc.contributor.author.fl_str_mv |
Sousa, Alessandra de Moraes |
dc.subject.por.fl_str_mv |
Metilxantina Sêmen Nelore Estresse oxidativo CNPQ::CIENCIAS AGRARIAS Methylxanthine Semen Nellore Oxidative stress |
topic |
Metilxantina Sêmen Nelore Estresse oxidativo CNPQ::CIENCIAS AGRARIAS Methylxanthine Semen Nellore Oxidative stress |
description |
The aim of the present study was to evaluate the effect of different concentrations of pentoxifylline on the viability, quality and oxidative stress in post-thawed and in vitro capacitated bovine sperm. Cryopreserved samples of nine Nellore bulls (Bos taurus indicus) were submitted to selection and sperm capacitation using a 45% and 90% Percoll® gradient. Samples were ressuspended in fertilization medium, and assigned in three treatments: control (miliQ water), 2.5 mM pentoxifylline and 5.0 mM pentoxifylline. Then, the samples were incubated in a water bath at 37 ° C for 3 minutes. After incubation, sperm motility and vigor, plasma membrane viability, mitochondrial cytochemical activity, acrosomal membrane integrity and oxidative stress were assessed. The data were analyzed by ANOVA, Wilcoxon and Tukey test. Significance level was set as 5% using SAS System version 9.2 software. Oxidative stress was higher in 2.5 mM treatment compared to the control and 5.0 mM pentoxifylline treatments (0.42 ± 0.06 0.28 ± 0.04 and 0.31 ± 0.04 ng / mL TBARS, respectively). A higher percentage of sperm with no mitochondrial cytochemical activity (DABIV) in 5.0 mM treatment (10.32 ± 1.39%) was was noticed in comparison to 2.5 mM treatment (5.89 ± 1.95%) and control (5.67 ± 1.17%). No differences were observed among treatments for the remaining variables. Therefore, the addition of pentoxifylline at 2.5mM and 5mM was not efficient to improve the sperm parameters of thawed bovine sperm, capacitated and submitted to in vitro incubation. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-07-20 2018-05-21 2021-05-13T19:42:35Z 2021-05-13T19:42:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SOUSA, Alessandra de Moraes. Incubação com pentoxifilina do espermatozoide bovino capacitado. 2018. 46 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Mato Grosso, Faculdade de Agronomia, Medicina Veterinária e Zootecnia, Cuiabá, 2018. http://ri.ufmt.br/handle/1/2507 |
identifier_str_mv |
SOUSA, Alessandra de Moraes. Incubação com pentoxifilina do espermatozoide bovino capacitado. 2018. 46 f. Dissertação (Mestrado em Ciência Animal) - Universidade Federal de Mato Grosso, Faculdade de Agronomia, Medicina Veterinária e Zootecnia, Cuiabá, 2018. |
url |
http://ri.ufmt.br/handle/1/2507 |
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por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Mato Grosso Brasil Faculdade de Agronomia, Medicina Veterinária e Zootecnia (FAMEVZ) UFMT CUC - Cuiabá Programa de Pós-Graduação em Ciência Animal |
publisher.none.fl_str_mv |
Universidade Federal de Mato Grosso Brasil Faculdade de Agronomia, Medicina Veterinária e Zootecnia (FAMEVZ) UFMT CUC - Cuiabá Programa de Pós-Graduação em Ciência Animal |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFMT instname:Universidade Federal de Mato Grosso (UFMT) instacron:UFMT |
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Universidade Federal de Mato Grosso (UFMT) |
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UFMT |
institution |
UFMT |
reponame_str |
Repositório Institucional da UFMT |
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Repositório Institucional da UFMT |
repository.name.fl_str_mv |
Repositório Institucional da UFMT - Universidade Federal de Mato Grosso (UFMT) |
repository.mail.fl_str_mv |
jordanbiblio@gmail.com |
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1804648505984155648 |