Avaliação do mecanismo de ação anti-inflamatória do extrato metanólico e fração hexânica de Cariniana rubra Gardner ex Miers

Detalhes bibliográficos
Autor(a) principal: Silva, Donata Norman Paulino Brandão
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFMT
Texto Completo: http://ri.ufmt.br/handle/1/487
Resumo: EVALUATION OF THE MECHANISM OF ACTION OF ANTI - INFLAMMATORY METHANOL EXTRACT AND HEXANE FRACTION OF Cariniana rubra Gardner ex miers. Silva D.N.P.B. Dissertation presented to the Coordination of the Post-Graduation Programs in Medicine of Faculty of Medicine of Federal University of Mato Grosso, as a partial requirement for the Master Degree of Health Sciences, Pharmacology Field. Advisor: Prof. Dr. Amilcar Sabino Damazo, Co -supervisor: Prof. Dr. Domingos Tabajara de Oliveira Martins. Cariniana rubra Gardner ex Miers is a tree that belongs to the family Lecythidhaeae This family is present in very different regions as areas with low flood periodically , mountainous regions with higher elevations to 1,000 feet above sea level clenched and regions with secondary vegetation ( capoeira ) formed after disturbances in natural environments . Currently has eight chemical compounds have been identified from the inner bark as phenolic compounds: β - sitosterol , stigmasterol , α and β-amirinas , arjunolic acid and sitosterol 3 - O- β - D - glucopiyanosil . Triterpenoid saponins : 28 - β - glucopyranosyl -23 - O- acetyl arjunolic acid , 3 - O- β - glucopyranosil arjunolic acid and 28 - O- [ α -L - rhamnopiranosil - ( 1 → 2 ) - β - glucopyranosil ] -23 - O- aceti0l arjunolic acid . Goal of this study was the validation of its anti-inflammatory properties, determining its mechanism of action and identification of secondary metabolites using in vivo and in vitro experimental models. To obtain the fraction of methanol extract was first subjected to liquid -liquid partition by suspending a mixture of methanol and distilled water. Then, hexane was added and after stirring the formation of the hexane fraction. Phytochemical screening revealed the existence of several secondary metabolites many of which are known anti - inflammatory action. It was possible to identify some compounds that are responsible for the anti-inflammatory action as by chromatographic and spectrometric analysis.The Hippocratic essay no animal death and organ analyzed did not change demonstrating that both EMCR as FrHCr not exhibit toxicity. In the cytotoxicity assay using cells of Chinese hamster ovary (CHO) by the method of Alamar Blue showed that both EMCR as FrHCr can be considered cytotoxic (IC50 ˂ 50 mg / ml). In an inflammation model by air pouch histological analysis demonstrated that the skin FrHCr have an EMCR and antimigratory effects of leukocytes and analysis of the washed leukocyte migration bubble demonstrated that treatment with EMCR was similar to standard drug , preventing migration PMN and MN cavity following administration of carrageenan . To understand the mechanism of action of the inflammatory process, we evaluated the release of the cytokines TNF - α, IL - 1β and IL-10 and expression of AnxA1. The AnxA1 protein expression was evaluated by immunohistochemistry data indicated that, after induction of inflammation, we observed an increase in anti-inflammatory protein AnxA1, particularly in neutrophils. The fact that EMCR induce expression of AnxA1 indicates that some phytochemical component of this plant induces the protein expression of this mediator, this being one of its possible anti-inflammatory mechanisms.
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spelling Avaliação do mecanismo de ação anti-inflamatória do extrato metanólico e fração hexânica de Cariniana rubra Gardner ex MiersCariniana rubraInflamaçãoBolha de arCNPQ::CIENCIAS DA SAUDE::MEDICINACariniana rubraInflammationAir pouchEVALUATION OF THE MECHANISM OF ACTION OF ANTI - INFLAMMATORY METHANOL EXTRACT AND HEXANE FRACTION OF Cariniana rubra Gardner ex miers. Silva D.N.P.B. Dissertation presented to the Coordination of the Post-Graduation Programs in Medicine of Faculty of Medicine of Federal University of Mato Grosso, as a partial requirement for the Master Degree of Health Sciences, Pharmacology Field. Advisor: Prof. Dr. Amilcar Sabino Damazo, Co -supervisor: Prof. Dr. Domingos Tabajara de Oliveira Martins. Cariniana rubra Gardner ex Miers is a tree that belongs to the family Lecythidhaeae This family is present in very different regions as areas with low flood periodically , mountainous regions with higher elevations to 1,000 feet above sea level clenched and regions with secondary vegetation ( capoeira ) formed after disturbances in natural environments . Currently has eight chemical compounds have been identified from the inner bark as phenolic compounds: β - sitosterol , stigmasterol , α and β-amirinas , arjunolic acid and sitosterol 3 - O- β - D - glucopiyanosil . Triterpenoid saponins : 28 - β - glucopyranosyl -23 - O- acetyl arjunolic acid , 3 - O- β - glucopyranosil arjunolic acid and 28 - O- [ α -L - rhamnopiranosil - ( 1 → 2 ) - β - glucopyranosil ] -23 - O- aceti0l arjunolic acid . Goal of this study was the validation of its anti-inflammatory properties, determining its mechanism of action and identification of secondary metabolites using in vivo and in vitro experimental models. To obtain the fraction of methanol extract was first subjected to liquid -liquid partition by suspending a mixture of methanol and distilled water. Then, hexane was added and after stirring the formation of the hexane fraction. Phytochemical screening revealed the existence of several secondary metabolites many of which are known anti - inflammatory action. It was possible to identify some compounds that are responsible for the anti-inflammatory action as by chromatographic and spectrometric analysis.The Hippocratic essay no animal death and organ analyzed did not change demonstrating that both EMCR as FrHCr not exhibit toxicity. In the cytotoxicity assay using cells of Chinese hamster ovary (CHO) by the method of Alamar Blue showed that both EMCR as FrHCr can be considered cytotoxic (IC50 ˂ 50 mg / ml). In an inflammation model by air pouch histological analysis demonstrated that the skin FrHCr have an EMCR and antimigratory effects of leukocytes and analysis of the washed leukocyte migration bubble demonstrated that treatment with EMCR was similar to standard drug , preventing migration PMN and MN cavity following administration of carrageenan . To understand the mechanism of action of the inflammatory process, we evaluated the release of the cytokines TNF - α, IL - 1β and IL-10 and expression of AnxA1. The AnxA1 protein expression was evaluated by immunohistochemistry data indicated that, after induction of inflammation, we observed an increase in anti-inflammatory protein AnxA1, particularly in neutrophils. The fact that EMCR induce expression of AnxA1 indicates that some phytochemical component of this plant induces the protein expression of this mediator, this being one of its possible anti-inflammatory mechanisms.AVALIAÇÃO DO MECANISMO DE AÇÃO ANTI-INFLAMATÓRIA DO EXTRATO METANÓLICO E FRAÇÃO HEXÂNICA DE Cariniana rubra Gardner ex Miers. Silva D.N.P.B. Dissertação apresentada à Coordenação do Programa de Pós-Graduação em Ciências da Saúde da Faculdade de Medicina da Universidade Federal de Mato Grosso, como requisito parcial para a obtenção do título de Mestre em Ciências da Saúde, Área de Farmacologia. Orientador: Prof. Dr. Amílcar Sabino Damazo; Co-orientador: Prof. Dr. Domingos Tabajara de Oliveira Martins. Cariniana rubra Gardner ex Miers é uma árvore que pertence à família Lecythidhaeae Esta família está presente em regiões muito diferentes como áreas baixas com inundação periodicamente, regiões montanhosas com elevações superiores a 1.000 metros acima do nível do mar, cerrados e regiões com vegetações secundárias (capoeiras) que se formam após distúrbios em ambientes naturais. Tem atualmente oito compostos químicos já identificados a partir da entrecasca como Compostos fenólicos: β-sitosterol, stigmasterol, α e β-amirinas, ácido arjunólico e sitosterol 3-O-β-D-glucopiranosil. Saponinas triterpenicas: 28-β-glucopiranosil-23-O-acetil acido arjunolico, 3-O-β-glucopiranosil acido arjunolico e 28-O-[α-L-Rhanopiranosil-(1→2)-β-glucopiranosil]-23-O-aceti0l acido arjunolico. O Objetivo deste trabalho foi a validação de suas propriedades anti-inflamatórias, determinação de seu mecanismo de ação e identificação dos seus metabólitos secundários utilizando modelos experimentais in vivo e in vitro. Para obter a fração o extrato metanólico foi inicialmente submetido à partição líquido-liquido através da suspensão de uma mistura de água destilada e de metanol. Em seguida, foi adicionado hexâno e após agitação a formação da fração hexânica. A fitoquímica revelou a existência de diversos metabólitos secundários muitos dos quais sabidamente de ação anti-inflamatória. Foi possível a identificação de alguns compostos que são responsáveis pela ação anti-inflamatória pela análise cromatográfica e espectrométrica. No ensaio hipocrático não houve morte dos animais e os órgão analisados não apresentaram alteração demostrando que tanto o EMCr quanto a FrHCr não apresentam toxicidade. No modelo de inflamação por bolha de ar as análises histológica da pele demonstraram que o EMCr e a FrHCr apresentam um efeito antimigratório de leucócitos e as análises do lavado da bolha a migração leucocitária demonstrou que o tratamento com EMCr foi semelhante a droga padrão, impedindo a migração de PMN e MN na cavidade, após a administração de carragenina. Para compreender o mecanismo de ação do processo inflamatório, foram avaliados a liberação das citocinas TNF-α, IL-1β e a IL10 e expressão da AnxA1. A expressão da proteína AnxA1 foi avaliada pela imunoistoquímica os dados indicaram que, após a indução do processo inflamatório, observamos um aumento da proteína anti-inflamatória AnxA1, particularmente nos neutrófilos. O fato do EMCr induzir a expressão da AnxA1 indica que algum componente fitoquímico dessa planta induz a expressão proteica deste mediador, sendo esse um de seus possíveis mecanismos anti-inflamatórios.Universidade Federal de Mato GrossoBrasilFaculdade de Medicina (FM)UFMT CUC - CuiabáPrograma de Pós-Graduação em Ciências da SaúdeDamazo, Amílcar SabinoMartins, Domingos Tabajara de Oliveirahttp://lattes.cnpq.br/3794477872946546http://lattes.cnpq.br/3708368867452889Damazo, Amílcar Sabino165.559.138-00http://lattes.cnpq.br/3708368867452889Andrade, Cláudia Marlise Balbinotti899.065.600-10http://lattes.cnpq.br/7129682665041036165.559.138-00.Vieira, Evanice Menezes Marçal899.065.600-10http://lattes.cnpq.br/8227577152556293Silva, Donata Norman Paulino Brandão2017-09-22T12:19:26Z2014-07-012017-09-22T12:19:26Z2014-02-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisSILVA, Donata Norman Paulino Brandão. Avaliação do mecanismo de ação anti-inflamatória do extrato metanólico e fração hexânica de Cariniana rubra Gardner ex Miers. 2014. 70 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal de Mato Grosso, Faculdade de Medicina, Cuiabá, 2014.http://ri.ufmt.br/handle/1/487porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMTinstname:Universidade Federal de Mato Grosso (UFMT)instacron:UFMT2017-09-26T07:01:02Zoai:localhost:1/487Repositório InstitucionalPUBhttp://ri.ufmt.br/oai/requestjordanbiblio@gmail.comopendoar:2017-09-26T07:01:02Repositório Institucional da UFMT - Universidade Federal de Mato Grosso (UFMT)false
dc.title.none.fl_str_mv Avaliação do mecanismo de ação anti-inflamatória do extrato metanólico e fração hexânica de Cariniana rubra Gardner ex Miers
title Avaliação do mecanismo de ação anti-inflamatória do extrato metanólico e fração hexânica de Cariniana rubra Gardner ex Miers
spellingShingle Avaliação do mecanismo de ação anti-inflamatória do extrato metanólico e fração hexânica de Cariniana rubra Gardner ex Miers
Silva, Donata Norman Paulino Brandão
Cariniana rubra
Inflamação
Bolha de ar
CNPQ::CIENCIAS DA SAUDE::MEDICINA
Cariniana rubra
Inflammation
Air pouch
title_short Avaliação do mecanismo de ação anti-inflamatória do extrato metanólico e fração hexânica de Cariniana rubra Gardner ex Miers
title_full Avaliação do mecanismo de ação anti-inflamatória do extrato metanólico e fração hexânica de Cariniana rubra Gardner ex Miers
title_fullStr Avaliação do mecanismo de ação anti-inflamatória do extrato metanólico e fração hexânica de Cariniana rubra Gardner ex Miers
title_full_unstemmed Avaliação do mecanismo de ação anti-inflamatória do extrato metanólico e fração hexânica de Cariniana rubra Gardner ex Miers
title_sort Avaliação do mecanismo de ação anti-inflamatória do extrato metanólico e fração hexânica de Cariniana rubra Gardner ex Miers
author Silva, Donata Norman Paulino Brandão
author_facet Silva, Donata Norman Paulino Brandão
author_role author
dc.contributor.none.fl_str_mv Damazo, Amílcar Sabino
Martins, Domingos Tabajara de Oliveira
http://lattes.cnpq.br/3794477872946546
http://lattes.cnpq.br/3708368867452889
Damazo, Amílcar Sabino
165.559.138-00
http://lattes.cnpq.br/3708368867452889
Andrade, Cláudia Marlise Balbinotti
899.065.600-10
http://lattes.cnpq.br/7129682665041036
165.559.138-00
.
Vieira, Evanice Menezes Marçal
899.065.600-10
http://lattes.cnpq.br/8227577152556293
dc.contributor.author.fl_str_mv Silva, Donata Norman Paulino Brandão
dc.subject.por.fl_str_mv Cariniana rubra
Inflamação
Bolha de ar
CNPQ::CIENCIAS DA SAUDE::MEDICINA
Cariniana rubra
Inflammation
Air pouch
topic Cariniana rubra
Inflamação
Bolha de ar
CNPQ::CIENCIAS DA SAUDE::MEDICINA
Cariniana rubra
Inflammation
Air pouch
description EVALUATION OF THE MECHANISM OF ACTION OF ANTI - INFLAMMATORY METHANOL EXTRACT AND HEXANE FRACTION OF Cariniana rubra Gardner ex miers. Silva D.N.P.B. Dissertation presented to the Coordination of the Post-Graduation Programs in Medicine of Faculty of Medicine of Federal University of Mato Grosso, as a partial requirement for the Master Degree of Health Sciences, Pharmacology Field. Advisor: Prof. Dr. Amilcar Sabino Damazo, Co -supervisor: Prof. Dr. Domingos Tabajara de Oliveira Martins. Cariniana rubra Gardner ex Miers is a tree that belongs to the family Lecythidhaeae This family is present in very different regions as areas with low flood periodically , mountainous regions with higher elevations to 1,000 feet above sea level clenched and regions with secondary vegetation ( capoeira ) formed after disturbances in natural environments . Currently has eight chemical compounds have been identified from the inner bark as phenolic compounds: β - sitosterol , stigmasterol , α and β-amirinas , arjunolic acid and sitosterol 3 - O- β - D - glucopiyanosil . Triterpenoid saponins : 28 - β - glucopyranosyl -23 - O- acetyl arjunolic acid , 3 - O- β - glucopyranosil arjunolic acid and 28 - O- [ α -L - rhamnopiranosil - ( 1 → 2 ) - β - glucopyranosil ] -23 - O- aceti0l arjunolic acid . Goal of this study was the validation of its anti-inflammatory properties, determining its mechanism of action and identification of secondary metabolites using in vivo and in vitro experimental models. To obtain the fraction of methanol extract was first subjected to liquid -liquid partition by suspending a mixture of methanol and distilled water. Then, hexane was added and after stirring the formation of the hexane fraction. Phytochemical screening revealed the existence of several secondary metabolites many of which are known anti - inflammatory action. It was possible to identify some compounds that are responsible for the anti-inflammatory action as by chromatographic and spectrometric analysis.The Hippocratic essay no animal death and organ analyzed did not change demonstrating that both EMCR as FrHCr not exhibit toxicity. In the cytotoxicity assay using cells of Chinese hamster ovary (CHO) by the method of Alamar Blue showed that both EMCR as FrHCr can be considered cytotoxic (IC50 ˂ 50 mg / ml). In an inflammation model by air pouch histological analysis demonstrated that the skin FrHCr have an EMCR and antimigratory effects of leukocytes and analysis of the washed leukocyte migration bubble demonstrated that treatment with EMCR was similar to standard drug , preventing migration PMN and MN cavity following administration of carrageenan . To understand the mechanism of action of the inflammatory process, we evaluated the release of the cytokines TNF - α, IL - 1β and IL-10 and expression of AnxA1. The AnxA1 protein expression was evaluated by immunohistochemistry data indicated that, after induction of inflammation, we observed an increase in anti-inflammatory protein AnxA1, particularly in neutrophils. The fact that EMCR induce expression of AnxA1 indicates that some phytochemical component of this plant induces the protein expression of this mediator, this being one of its possible anti-inflammatory mechanisms.
publishDate 2014
dc.date.none.fl_str_mv 2014-07-01
2014-02-28
2017-09-22T12:19:26Z
2017-09-22T12:19:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv SILVA, Donata Norman Paulino Brandão. Avaliação do mecanismo de ação anti-inflamatória do extrato metanólico e fração hexânica de Cariniana rubra Gardner ex Miers. 2014. 70 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal de Mato Grosso, Faculdade de Medicina, Cuiabá, 2014.
http://ri.ufmt.br/handle/1/487
identifier_str_mv SILVA, Donata Norman Paulino Brandão. Avaliação do mecanismo de ação anti-inflamatória do extrato metanólico e fração hexânica de Cariniana rubra Gardner ex Miers. 2014. 70 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal de Mato Grosso, Faculdade de Medicina, Cuiabá, 2014.
url http://ri.ufmt.br/handle/1/487
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Mato Grosso
Brasil
Faculdade de Medicina (FM)
UFMT CUC - Cuiabá
Programa de Pós-Graduação em Ciências da Saúde
publisher.none.fl_str_mv Universidade Federal de Mato Grosso
Brasil
Faculdade de Medicina (FM)
UFMT CUC - Cuiabá
Programa de Pós-Graduação em Ciências da Saúde
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMT
instname:Universidade Federal de Mato Grosso (UFMT)
instacron:UFMT
instname_str Universidade Federal de Mato Grosso (UFMT)
instacron_str UFMT
institution UFMT
reponame_str Repositório Institucional da UFMT
collection Repositório Institucional da UFMT
repository.name.fl_str_mv Repositório Institucional da UFMT - Universidade Federal de Mato Grosso (UFMT)
repository.mail.fl_str_mv jordanbiblio@gmail.com
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