Caracterização citotaxonômica de Synbranchus Bloch, 1795 (Teleostei, Synbranchidae) da Bacia do Alto Paraguai

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Mamynne Correa da Costa
Data de Publicação: 2017
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFMT
Texto Completo: http://ri.ufmt.br/handle/1/4249
Resumo: Synbranchus Bloch, 1795 (Synbranchidae), popularly known as Muçum, is represented by three species, S. marmoratus Bloch, 1785, S. lampreia Favorito, Zanata & Assumpção, 2005 and S. madeirae Rosen and Rumney, 1972. These fish have great commercial importance in the region, as it is sold as baits for sport fishing. From the cytogenetic point of view, among the representatives already studied at Mato Grosso's Pantanal, three karyotypic patterns are verified in the genus: karyotypes with 2n = 42, 2n = 44 and 2n = 46 chromosomes, suggesting the existence of at least three distinct species. Therefore, the goal of the present work is to characterize cytogenetically by classical and molecular methods exemplary from different regions of the northern Mato Grosso's Pantanal, in order to confirm this information and verify how many and which are the species of Synbranchus that actually occur in the region and that are used by fishermen, in the trade of live baits. They collected 54 specimens in the regions of Pantanal in Poconé, Cáceres and Barão de Melgaço, which were submitted to classical and molecular cytogenetic procedures, and morphological and morphometric analysis. The occurrence of the three karyotypes was confirmed, with diploid numbers 2n = 42 chromosomes (4 m + 14 st + 24 a) represented the species Synbranchus aff. marmoratus, with a specimen with 2n = 43 chromosomes, indicating the occurrence of a supernumerary chromosome; 2n = 44 chromosomes (4 m + 2 sm + 2 st + 36 a), represented the species S. aff. lampreia, and 2n = 46 chromosomes (6 m + 2 sm + 10 st + 28 a), represented the species S. madeirae. The band C revealed markings heterochromatin constitutive in the centromeric and interstitial regions of almost all chromosomes, the supernumerary chromosome is completely heterochromatic. The nucleoli organizing regions were detected in different chromosomal portions, in the three cytotypes found. In the specimens with 2n = 42, these sites were located on the short arm of the acrocentric pair 14; in the specimens with 2n = 44 were located in the long arm of the acrocentric pair 5, and in the fish with 2n = 46 were located in the short arm of the metacentric pair 2. Fluorescent in situ hybridization (FISH) using the 5S rDNA probes revealed markings on a single pair of acrocentric chromosomes. The 18S rDNA probe revealed distinct patterns among the karyotypes analyzed coinciding with the AgRONs markers. Telomere probes revealed signals in the terminal regions of almost all chromosomes. PCA performed from the morphometry data did not differentiate the species; however, the pattern of staining and shape of the head, as well as cytogenetic data confirmed that there are at least three taxonomic units for the genus Synbranchus in the studied region, which need either to be described or be correctly identified.
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spelling Caracterização citotaxonômica de Synbranchus Bloch, 1795 (Teleostei, Synbranchidae) da Bacia do Alto ParaguaiMuçumIscas vivasCariotiposSynbranchus madeiraeCNPQ::CIENCIAS BIOLOGICAS::ECOLOGIAMuçumLive baitsKaryotypesSynbranchus madeiraeSynbranchus Bloch, 1795 (Synbranchidae), popularly known as Muçum, is represented by three species, S. marmoratus Bloch, 1785, S. lampreia Favorito, Zanata & Assumpção, 2005 and S. madeirae Rosen and Rumney, 1972. These fish have great commercial importance in the region, as it is sold as baits for sport fishing. From the cytogenetic point of view, among the representatives already studied at Mato Grosso's Pantanal, three karyotypic patterns are verified in the genus: karyotypes with 2n = 42, 2n = 44 and 2n = 46 chromosomes, suggesting the existence of at least three distinct species. Therefore, the goal of the present work is to characterize cytogenetically by classical and molecular methods exemplary from different regions of the northern Mato Grosso's Pantanal, in order to confirm this information and verify how many and which are the species of Synbranchus that actually occur in the region and that are used by fishermen, in the trade of live baits. They collected 54 specimens in the regions of Pantanal in Poconé, Cáceres and Barão de Melgaço, which were submitted to classical and molecular cytogenetic procedures, and morphological and morphometric analysis. The occurrence of the three karyotypes was confirmed, with diploid numbers 2n = 42 chromosomes (4 m + 14 st + 24 a) represented the species Synbranchus aff. marmoratus, with a specimen with 2n = 43 chromosomes, indicating the occurrence of a supernumerary chromosome; 2n = 44 chromosomes (4 m + 2 sm + 2 st + 36 a), represented the species S. aff. lampreia, and 2n = 46 chromosomes (6 m + 2 sm + 10 st + 28 a), represented the species S. madeirae. The band C revealed markings heterochromatin constitutive in the centromeric and interstitial regions of almost all chromosomes, the supernumerary chromosome is completely heterochromatic. The nucleoli organizing regions were detected in different chromosomal portions, in the three cytotypes found. In the specimens with 2n = 42, these sites were located on the short arm of the acrocentric pair 14; in the specimens with 2n = 44 were located in the long arm of the acrocentric pair 5, and in the fish with 2n = 46 were located in the short arm of the metacentric pair 2. Fluorescent in situ hybridization (FISH) using the 5S rDNA probes revealed markings on a single pair of acrocentric chromosomes. The 18S rDNA probe revealed distinct patterns among the karyotypes analyzed coinciding with the AgRONs markers. Telomere probes revealed signals in the terminal regions of almost all chromosomes. PCA performed from the morphometry data did not differentiate the species; however, the pattern of staining and shape of the head, as well as cytogenetic data confirmed that there are at least three taxonomic units for the genus Synbranchus in the studied region, which need either to be described or be correctly identified.CNPqSynbranchus Bloch, 1795 (Synbranchidae), popularmente conhecido como muçum, é representado por três espécies: S. marmoratus Bloch, 1785, S. lampreia Favorito, Zanata & Assumpção, 2005 e S. madeirae Rosen & Rumney, 1972. Esses peixes tem uma grande importância comercial na região do Pantanal, pois é vendida como isca-viva para pesca esportiva e predatória. Do ponto de vista citogenético, entre os representantes já estudados no Pantanal de Mato Grosso, três padrões cariotípicos são verificados no gênero: cariótipos com 2n=42, 2n=44 e 2n=46 cromossomos, sugerindo a existência de pelo menos três espécies distintas. Diante disso, o objetivo do presente trabalho é caracterizar citogeneticamente por métodos clássicos e moleculares exemplares procedentes de diferentes regiões do norte do Pantanal de Mato Grosso, no sentido de se confirmar essas informações e verificar quantas e quais são as espécies de Synbranchus que de fato ocorrem na região e que são utilizadas pelos pescadores, no comércio de iscas vivas. Foram coletados 54 espécimes nas regiões do Pantanal de Poconé, Cáceres e Barão de Melgaço, que foram submetidos aos procedimentos de citogenética clássica e molecular e análises morfológicas e morfométricas. Foram confirmadas as ocorrências dos três cariótipos citados, com números diplóides 2n= 42 cromossomos (4 m + 14 st + 24 a) representado a espécie aqui referenciada como Synbranchus aff. marmoratus, com um indivíduo com 2n=43 cromossomos (indicando a ocorrência de um cromossomo supranumerário na espécie); 2n= 44 cromossomos (4 m + 2 sm + 2 st + 36 a), representando a espécie aqui citada como S. aff. lampreia, e 2n=46 cromossomos (6 m + 2 sm + 10 st + 28 a), representando a espécie S. madeirae. Em todas as espécies analisadas, a banda C revelou marcações de heterocromatina constitutivas nas regiões centroméricas e intersticiais de quase todos os cromossomos, o cromossomo supranumerário é completamente heterocromático. As regiões organizadoras de nucléolos foram detectadas em diferentes porções cromossômicas, nas três espécies encontradas. Nos exemplares com 2n=42, esses sítios foram localizados no braço curto do par 14 acrocêntrico; nos exemplares com 2n=44 foram localizados no braço longo do par 5 acrocêntrico, e nos peixes com 2n=46 foram localizados no braço curto do par 2 metacêntrico. A hibridação in situ por Fluorenscência (FISH) utilizando as sondas de DNAr 5S revelaram marcações em um único par de cromossomos acrocêntricos. A sonda de DNAr 18S revelou padrões distintos entre os cariótipos analisados coincidentes com a marcações das AgRONs. As sondas teloméricas revelaram sinais nas regiões terminais de quase todos os cromossomos. A PCA realizada a partir dos dados de morfometria não diferenciou os indivíduos; no entanto, o padrão de coloração e formato da cabeça, assim como os dados citogenéticos confirmam a ocorrência de três unidades taxonômicas para o gênero Synbranchus na região de estudo, as quais necessitam, ou serem descritas, ou serem corretamente identificadas.Universidade Federal de Mato GrossoBrasilInstituto de Biociências (IB)UFMT CUC - CuiabáPrograma de Pós-Graduação em Ecologia e Conservação da BiodiversidadeVenere, Paulo Césarhttp://lattes.cnpq.br/0397087873707987Venere, Paulo César108.904.178-06http://lattes.cnpq.br/0397087873707987Ferreira, Daniela Cristina195.711.258-14http://lattes.cnpq.br/4880526235013142108.904.178-06Carvalho, Fernando Rogério de215.121.008-11http://lattes.cnpq.br/2546070466753742Porto, Jorge Ivan Rebelo173.773.582-20http://lattes.cnpq.br/7563186505089160Rodrigues, Mamynne Correa da Costa2023-06-21T16:29:24Z20172023-06-21T16:29:24Z2017-03-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisRODRIGUES, Mamynne Correa da Costa. Caracterização citotaxonômica de Synbranchus Bloch, 1795 (Teleostei, Synbranchidae) da Bacia do Alto Paraguai. 2017. 49 f. Dissertação (Mestrado em Ecologia e Conservação da Biodiversidade) - Universidade Federal de Mato Grosso, Instituto de Biociências, Cuiabá, 2017.http://ri.ufmt.br/handle/1/4249porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMTinstname:Universidade Federal de Mato Grosso (UFMT)instacron:UFMT2023-06-23T07:11:15Zoai:localhost:1/4249Repositório InstitucionalPUBhttp://ri.ufmt.br/oai/requestjordanbiblio@gmail.comopendoar:2023-06-23T07:11:15Repositório Institucional da UFMT - Universidade Federal de Mato Grosso (UFMT)false
dc.title.none.fl_str_mv Caracterização citotaxonômica de Synbranchus Bloch, 1795 (Teleostei, Synbranchidae) da Bacia do Alto Paraguai
title Caracterização citotaxonômica de Synbranchus Bloch, 1795 (Teleostei, Synbranchidae) da Bacia do Alto Paraguai
spellingShingle Caracterização citotaxonômica de Synbranchus Bloch, 1795 (Teleostei, Synbranchidae) da Bacia do Alto Paraguai
Rodrigues, Mamynne Correa da Costa
Muçum
Iscas vivas
Cariotipos
Synbranchus madeirae
CNPQ::CIENCIAS BIOLOGICAS::ECOLOGIA
Muçum
Live baits
Karyotypes
Synbranchus madeirae
title_short Caracterização citotaxonômica de Synbranchus Bloch, 1795 (Teleostei, Synbranchidae) da Bacia do Alto Paraguai
title_full Caracterização citotaxonômica de Synbranchus Bloch, 1795 (Teleostei, Synbranchidae) da Bacia do Alto Paraguai
title_fullStr Caracterização citotaxonômica de Synbranchus Bloch, 1795 (Teleostei, Synbranchidae) da Bacia do Alto Paraguai
title_full_unstemmed Caracterização citotaxonômica de Synbranchus Bloch, 1795 (Teleostei, Synbranchidae) da Bacia do Alto Paraguai
title_sort Caracterização citotaxonômica de Synbranchus Bloch, 1795 (Teleostei, Synbranchidae) da Bacia do Alto Paraguai
author Rodrigues, Mamynne Correa da Costa
author_facet Rodrigues, Mamynne Correa da Costa
author_role author
dc.contributor.none.fl_str_mv Venere, Paulo César
http://lattes.cnpq.br/0397087873707987
Venere, Paulo César
108.904.178-06
http://lattes.cnpq.br/0397087873707987
Ferreira, Daniela Cristina
195.711.258-14
http://lattes.cnpq.br/4880526235013142
108.904.178-06
Carvalho, Fernando Rogério de
215.121.008-11
http://lattes.cnpq.br/2546070466753742
Porto, Jorge Ivan Rebelo
173.773.582-20
http://lattes.cnpq.br/7563186505089160
dc.contributor.author.fl_str_mv Rodrigues, Mamynne Correa da Costa
dc.subject.por.fl_str_mv Muçum
Iscas vivas
Cariotipos
Synbranchus madeirae
CNPQ::CIENCIAS BIOLOGICAS::ECOLOGIA
Muçum
Live baits
Karyotypes
Synbranchus madeirae
topic Muçum
Iscas vivas
Cariotipos
Synbranchus madeirae
CNPQ::CIENCIAS BIOLOGICAS::ECOLOGIA
Muçum
Live baits
Karyotypes
Synbranchus madeirae
description Synbranchus Bloch, 1795 (Synbranchidae), popularly known as Muçum, is represented by three species, S. marmoratus Bloch, 1785, S. lampreia Favorito, Zanata & Assumpção, 2005 and S. madeirae Rosen and Rumney, 1972. These fish have great commercial importance in the region, as it is sold as baits for sport fishing. From the cytogenetic point of view, among the representatives already studied at Mato Grosso's Pantanal, three karyotypic patterns are verified in the genus: karyotypes with 2n = 42, 2n = 44 and 2n = 46 chromosomes, suggesting the existence of at least three distinct species. Therefore, the goal of the present work is to characterize cytogenetically by classical and molecular methods exemplary from different regions of the northern Mato Grosso's Pantanal, in order to confirm this information and verify how many and which are the species of Synbranchus that actually occur in the region and that are used by fishermen, in the trade of live baits. They collected 54 specimens in the regions of Pantanal in Poconé, Cáceres and Barão de Melgaço, which were submitted to classical and molecular cytogenetic procedures, and morphological and morphometric analysis. The occurrence of the three karyotypes was confirmed, with diploid numbers 2n = 42 chromosomes (4 m + 14 st + 24 a) represented the species Synbranchus aff. marmoratus, with a specimen with 2n = 43 chromosomes, indicating the occurrence of a supernumerary chromosome; 2n = 44 chromosomes (4 m + 2 sm + 2 st + 36 a), represented the species S. aff. lampreia, and 2n = 46 chromosomes (6 m + 2 sm + 10 st + 28 a), represented the species S. madeirae. The band C revealed markings heterochromatin constitutive in the centromeric and interstitial regions of almost all chromosomes, the supernumerary chromosome is completely heterochromatic. The nucleoli organizing regions were detected in different chromosomal portions, in the three cytotypes found. In the specimens with 2n = 42, these sites were located on the short arm of the acrocentric pair 14; in the specimens with 2n = 44 were located in the long arm of the acrocentric pair 5, and in the fish with 2n = 46 were located in the short arm of the metacentric pair 2. Fluorescent in situ hybridization (FISH) using the 5S rDNA probes revealed markings on a single pair of acrocentric chromosomes. The 18S rDNA probe revealed distinct patterns among the karyotypes analyzed coinciding with the AgRONs markers. Telomere probes revealed signals in the terminal regions of almost all chromosomes. PCA performed from the morphometry data did not differentiate the species; however, the pattern of staining and shape of the head, as well as cytogenetic data confirmed that there are at least three taxonomic units for the genus Synbranchus in the studied region, which need either to be described or be correctly identified.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-03-27
2023-06-21T16:29:24Z
2023-06-21T16:29:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv RODRIGUES, Mamynne Correa da Costa. Caracterização citotaxonômica de Synbranchus Bloch, 1795 (Teleostei, Synbranchidae) da Bacia do Alto Paraguai. 2017. 49 f. Dissertação (Mestrado em Ecologia e Conservação da Biodiversidade) - Universidade Federal de Mato Grosso, Instituto de Biociências, Cuiabá, 2017.
http://ri.ufmt.br/handle/1/4249
identifier_str_mv RODRIGUES, Mamynne Correa da Costa. Caracterização citotaxonômica de Synbranchus Bloch, 1795 (Teleostei, Synbranchidae) da Bacia do Alto Paraguai. 2017. 49 f. Dissertação (Mestrado em Ecologia e Conservação da Biodiversidade) - Universidade Federal de Mato Grosso, Instituto de Biociências, Cuiabá, 2017.
url http://ri.ufmt.br/handle/1/4249
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Mato Grosso
Brasil
Instituto de Biociências (IB)
UFMT CUC - Cuiabá
Programa de Pós-Graduação em Ecologia e Conservação da Biodiversidade
publisher.none.fl_str_mv Universidade Federal de Mato Grosso
Brasil
Instituto de Biociências (IB)
UFMT CUC - Cuiabá
Programa de Pós-Graduação em Ecologia e Conservação da Biodiversidade
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMT
instname:Universidade Federal de Mato Grosso (UFMT)
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instname_str Universidade Federal de Mato Grosso (UFMT)
instacron_str UFMT
institution UFMT
reponame_str Repositório Institucional da UFMT
collection Repositório Institucional da UFMT
repository.name.fl_str_mv Repositório Institucional da UFMT - Universidade Federal de Mato Grosso (UFMT)
repository.mail.fl_str_mv jordanbiblio@gmail.com
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