Efeito da desnutrição perinatal sobre o comportamento alimentar e função termogênica em ratos adultos

Detalhes bibliográficos
Autor(a) principal: Miranda, Ginislene Dias Souza
Data de Publicação: 2020
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFMT
Texto Completo: http://ri.ufmt.br/handle/1/4843
Resumo: Early nutritional imbalances are associated with metabolic diseases development in adulthood. It seems to be closely related to neuroendocrine modulations due to nutritional scarcity and/or hormonal changes. In the present study, we aimed to evaluate the effect of maternal calorie restriction during the first 2/3 of breastfeeding on the adult rat-offspring’s feeding behavior, biometrical, biochemical and thermogenesis parameters. Wistar rats underwent dietary restriction (50%) during the initial 2/3 of lactation (FR50 group) the control rats (Cont group) were fed ad libitum throughout lactation. At birth, litter size was adjusted to 8 pups. Ratoffspring milk ingestion was evaluated at 6th, 11th and 16th ages. At 12th day of lactation, milk was collected to creamatocrit and biochemical assessment and at 22-days-old rat-offspring were weaned. Body weight and food and water intake were evaluated every two days. At 40-daysold, the feeding preference was evaluated through the simultaneous offer of standard chow (normal fat-diet, NFD) and hypercaloric diet (high fat-diet, HFD) by 10 days, and at 90-daysold, individual food intake in dark cycle was assessed. At 100-days-old, rat-offspring were euthanized to collect blood sample and white and brown adipose tissue (BAT), as well as skeletal muscle to biochemical, biometrical and molecular assessment. Compared to milk from Cont group, in FR50’s milk was found increased values of glucose (72.52%, P<0.01), total cholesterol (79.80%, P<0.05), triglycerides (30.10%, P<0.05), total protein (53.17%, P<0.05) and energy content (29.35%, P<0.01). Regarding rat-offspring, FR50 rats displayed lean phenotype (17.82%, P<0.001) associated to high BAT mass (P<0.05), hyperphagia (12.59%, P<0.01) and preference for HFD ingestion (+68.27%, P<0.01). In relation to Cont group, FR50 rats increased the values of total-cholesterol (33.12%, P<0.001), triglycerides (26.88%, P<0.05), LDL-cholesterol (52.96%, P<0.001), VLDL-cholesterol (35.90%, P<0.01), and reduced by 8.51% the HDL-cholesterol values (P<0.05). In addition, RA50 rats showed an increase in Castelli indexes (P <0.001).While the protein expression of β3-AR was reduced by 40% (P<0.05), the expression of UCP1 was increased by 46.28% (P<0.05) in BAT. In conclusion, even though adult FR50 rats remain phenotypically lean, displayed hyperphagia and HFD preference as well as atherogenic high risk that is suggestive to consequent development of dyslipidemia-associated vascular diseases and high thermogenesis capacity in the BAT.
id UFMT_afdb9fb97c6fb9e098f89e5d26ed0c5d
oai_identifier_str oai:localhost:1/4843
network_acronym_str UFMT
network_name_str Repositório Institucional da UFMT
repository_id_str
spelling Efeito da desnutrição perinatal sobre o comportamento alimentar e função termogênica em ratos adultosDesnutrição calóricaProgramação metabólicaTermogêneseUCP1CNPQ::CIENCIAS DA SAUDECaloric malnutritionMetabolic programmingThermogenesisUCP1Early nutritional imbalances are associated with metabolic diseases development in adulthood. It seems to be closely related to neuroendocrine modulations due to nutritional scarcity and/or hormonal changes. In the present study, we aimed to evaluate the effect of maternal calorie restriction during the first 2/3 of breastfeeding on the adult rat-offspring’s feeding behavior, biometrical, biochemical and thermogenesis parameters. Wistar rats underwent dietary restriction (50%) during the initial 2/3 of lactation (FR50 group) the control rats (Cont group) were fed ad libitum throughout lactation. At birth, litter size was adjusted to 8 pups. Ratoffspring milk ingestion was evaluated at 6th, 11th and 16th ages. At 12th day of lactation, milk was collected to creamatocrit and biochemical assessment and at 22-days-old rat-offspring were weaned. Body weight and food and water intake were evaluated every two days. At 40-daysold, the feeding preference was evaluated through the simultaneous offer of standard chow (normal fat-diet, NFD) and hypercaloric diet (high fat-diet, HFD) by 10 days, and at 90-daysold, individual food intake in dark cycle was assessed. At 100-days-old, rat-offspring were euthanized to collect blood sample and white and brown adipose tissue (BAT), as well as skeletal muscle to biochemical, biometrical and molecular assessment. Compared to milk from Cont group, in FR50’s milk was found increased values of glucose (72.52%, P<0.01), total cholesterol (79.80%, P<0.05), triglycerides (30.10%, P<0.05), total protein (53.17%, P<0.05) and energy content (29.35%, P<0.01). Regarding rat-offspring, FR50 rats displayed lean phenotype (17.82%, P<0.001) associated to high BAT mass (P<0.05), hyperphagia (12.59%, P<0.01) and preference for HFD ingestion (+68.27%, P<0.01). In relation to Cont group, FR50 rats increased the values of total-cholesterol (33.12%, P<0.001), triglycerides (26.88%, P<0.05), LDL-cholesterol (52.96%, P<0.001), VLDL-cholesterol (35.90%, P<0.01), and reduced by 8.51% the HDL-cholesterol values (P<0.05). In addition, RA50 rats showed an increase in Castelli indexes (P <0.001).While the protein expression of β3-AR was reduced by 40% (P<0.05), the expression of UCP1 was increased by 46.28% (P<0.05) in BAT. In conclusion, even though adult FR50 rats remain phenotypically lean, displayed hyperphagia and HFD preference as well as atherogenic high risk that is suggestive to consequent development of dyslipidemia-associated vascular diseases and high thermogenesis capacity in the BAT.CAPESDesequilíbrios nutricionais no início da vida estão associados ao desenvolvimento de doenças metabólicas na vida adulta. Este fato parece estar intimamente relacionado a modulações neuroendócrinas decorrentes da carência nutricional e/ou alteração hormonal. No presente estudo objetivamos avaliar o efeito da restrição calórica materna, durante 2/3 iniciais de aleitamento, sobre o comportamento alimentar, parâmetros biométricos, bioquímicos e função termogênica da prole. Ratas Wistar foram submetidas a restrição alimentar (50%) durante os 2/3 iniciais da lactação (grupo RA50), as ratas controles (grupo Cont) receberam alimentação ad libitum durante toda a lactação. Ao nascimento, o tamanho da ninhada foi ajustado para 8 filhotes. Aos 6º, 11º e 16º dias de vida a ingestão de leite pela prole foi avaliada. Ao 12º dia de aleitamento, retirou-se amostra de leite para avaliação do crematócrito e parâmetros bioquímicos e ao 22º dia, realizou-se o desmame. O peso corporal e ingestão alimentar foram avaliados a cada dois dias. Aos 40 dias de vida, avaliou-se a preferência alimentar, através da oferta simultânea de ração padrão para roedores (normal fat-diet, NFD) e ração hipercalórica (high fat-diet, HFD) por 10 dias e aos 90 dias de vida, avaliou-se a ingestão alimentar individual no ciclo escuro. Aos 100 dias de vida, os ratos foram eutanasiados para a coleta de sangue, tecido adiposo branco e marrom (TAM) e muscular esquelético para análises bioquímicas, biométricas e moleculares. Em relação ao leite do grupo Cont, observou-se, em ratas RA50, aumento nos valores de glicose (72,52%, P<0,01), colesterol total (79,80%, P<0,05), triglicérides (30,10%, P<0,05), proteínas totais (53,17%, P<0,05) e conteúdo energético (29,35%, P<0,01). Quanto à prole, os ratos RA50 apresentaram fenótipo magro (17,82%, P<0,001) associado a maior massa de TAM (P<0,05), hiperfagia (12,59%, P<0,01) e preferência por ingestão da HFD (+68,27%, P<0,01). Em relação ao grupo Cont, os ratos RA50 tiveram aumento nos valores plasmático de colesterol total (33,12%, P<0,001), triglicérides (26,88%, P<0,05), colesterol-LDL (52,96%, P<0,001), colesterol-VLDL (35,90%, P<0,01), e redução de 8,51% no colesterol-HDL (P<0,05). Em adição, os ratos RA50 apresentaram aumento nos índices Castelli (P<0,001). Enquanto a expressão proteica do receptor adrenérgico beta 3 (β3-AR) foi reduzida em 40% (P<0,05), a expressão da UCP1 foi aumentada em 46,28% (P<0,05) no TAM. Concluímos que ratos RA50 adultos, embora mantenham-se fenotipicamente magros, apresentam hiperfagia e preferência por HFD assim como também um alto risco aterogênico sugestivo de consequente desenvolvimento de doenças vasculares associadas a dislipidemia e maior capacidade termogênica no TAM.Universidade Federal de Mato GrossoBrasilInstituto de Ciências da Saúde (ICS) - SinopUFMT CUS - SinopPrograma de Pós-Graduação em Ciências em SaúdeOliveira, Júlio Cezar dehttp://lattes.cnpq.br/0189760778239540Oliveira, Júlio Cezar de039.925.254-14http://lattes.cnpq.br/0189760778239540Oliveira, Ricardo de282.298.158-28http://lattes.cnpq.br/3181094624892559039.925.254-14Mathias, Paulo Cezar de Freitas747.050.088-04http://lattes.cnpq.br/5279465385771687Miranda, Ginislene Dias Souza2023-11-21T15:54:19Z2020-11-052023-11-21T15:54:19Z2020-07-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisMIRANDA, Ginislene Dias Souza. Efeito da desnutrição perinatal sobre o comportamento alimentar e função termogênica em ratos adultos. 2020. 74 f. Dissertação (Mestrado em Ciências em Saúde) - Universidade Federal de Mato Grosso, Campus Universitário de Sinop, Instituto de Ciências da Saúde, Sinop, 2020.http://ri.ufmt.br/handle/1/4843porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMTinstname:Universidade Federal de Mato Grosso (UFMT)instacron:UFMT2023-11-22T06:01:38Zoai:localhost:1/4843Repositório InstitucionalPUBhttp://ri.ufmt.br/oai/requestjordanbiblio@gmail.comopendoar:2023-11-22T06:01:38Repositório Institucional da UFMT - Universidade Federal de Mato Grosso (UFMT)false
dc.title.none.fl_str_mv Efeito da desnutrição perinatal sobre o comportamento alimentar e função termogênica em ratos adultos
title Efeito da desnutrição perinatal sobre o comportamento alimentar e função termogênica em ratos adultos
spellingShingle Efeito da desnutrição perinatal sobre o comportamento alimentar e função termogênica em ratos adultos
Miranda, Ginislene Dias Souza
Desnutrição calórica
Programação metabólica
Termogênese
UCP1
CNPQ::CIENCIAS DA SAUDE
Caloric malnutrition
Metabolic programming
Thermogenesis
UCP1
title_short Efeito da desnutrição perinatal sobre o comportamento alimentar e função termogênica em ratos adultos
title_full Efeito da desnutrição perinatal sobre o comportamento alimentar e função termogênica em ratos adultos
title_fullStr Efeito da desnutrição perinatal sobre o comportamento alimentar e função termogênica em ratos adultos
title_full_unstemmed Efeito da desnutrição perinatal sobre o comportamento alimentar e função termogênica em ratos adultos
title_sort Efeito da desnutrição perinatal sobre o comportamento alimentar e função termogênica em ratos adultos
author Miranda, Ginislene Dias Souza
author_facet Miranda, Ginislene Dias Souza
author_role author
dc.contributor.none.fl_str_mv Oliveira, Júlio Cezar de
http://lattes.cnpq.br/0189760778239540
Oliveira, Júlio Cezar de
039.925.254-14
http://lattes.cnpq.br/0189760778239540
Oliveira, Ricardo de
282.298.158-28
http://lattes.cnpq.br/3181094624892559
039.925.254-14
Mathias, Paulo Cezar de Freitas
747.050.088-04
http://lattes.cnpq.br/5279465385771687
dc.contributor.author.fl_str_mv Miranda, Ginislene Dias Souza
dc.subject.por.fl_str_mv Desnutrição calórica
Programação metabólica
Termogênese
UCP1
CNPQ::CIENCIAS DA SAUDE
Caloric malnutrition
Metabolic programming
Thermogenesis
UCP1
topic Desnutrição calórica
Programação metabólica
Termogênese
UCP1
CNPQ::CIENCIAS DA SAUDE
Caloric malnutrition
Metabolic programming
Thermogenesis
UCP1
description Early nutritional imbalances are associated with metabolic diseases development in adulthood. It seems to be closely related to neuroendocrine modulations due to nutritional scarcity and/or hormonal changes. In the present study, we aimed to evaluate the effect of maternal calorie restriction during the first 2/3 of breastfeeding on the adult rat-offspring’s feeding behavior, biometrical, biochemical and thermogenesis parameters. Wistar rats underwent dietary restriction (50%) during the initial 2/3 of lactation (FR50 group) the control rats (Cont group) were fed ad libitum throughout lactation. At birth, litter size was adjusted to 8 pups. Ratoffspring milk ingestion was evaluated at 6th, 11th and 16th ages. At 12th day of lactation, milk was collected to creamatocrit and biochemical assessment and at 22-days-old rat-offspring were weaned. Body weight and food and water intake were evaluated every two days. At 40-daysold, the feeding preference was evaluated through the simultaneous offer of standard chow (normal fat-diet, NFD) and hypercaloric diet (high fat-diet, HFD) by 10 days, and at 90-daysold, individual food intake in dark cycle was assessed. At 100-days-old, rat-offspring were euthanized to collect blood sample and white and brown adipose tissue (BAT), as well as skeletal muscle to biochemical, biometrical and molecular assessment. Compared to milk from Cont group, in FR50’s milk was found increased values of glucose (72.52%, P<0.01), total cholesterol (79.80%, P<0.05), triglycerides (30.10%, P<0.05), total protein (53.17%, P<0.05) and energy content (29.35%, P<0.01). Regarding rat-offspring, FR50 rats displayed lean phenotype (17.82%, P<0.001) associated to high BAT mass (P<0.05), hyperphagia (12.59%, P<0.01) and preference for HFD ingestion (+68.27%, P<0.01). In relation to Cont group, FR50 rats increased the values of total-cholesterol (33.12%, P<0.001), triglycerides (26.88%, P<0.05), LDL-cholesterol (52.96%, P<0.001), VLDL-cholesterol (35.90%, P<0.01), and reduced by 8.51% the HDL-cholesterol values (P<0.05). In addition, RA50 rats showed an increase in Castelli indexes (P <0.001).While the protein expression of β3-AR was reduced by 40% (P<0.05), the expression of UCP1 was increased by 46.28% (P<0.05) in BAT. In conclusion, even though adult FR50 rats remain phenotypically lean, displayed hyperphagia and HFD preference as well as atherogenic high risk that is suggestive to consequent development of dyslipidemia-associated vascular diseases and high thermogenesis capacity in the BAT.
publishDate 2020
dc.date.none.fl_str_mv 2020-11-05
2020-07-30
2023-11-21T15:54:19Z
2023-11-21T15:54:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv MIRANDA, Ginislene Dias Souza. Efeito da desnutrição perinatal sobre o comportamento alimentar e função termogênica em ratos adultos. 2020. 74 f. Dissertação (Mestrado em Ciências em Saúde) - Universidade Federal de Mato Grosso, Campus Universitário de Sinop, Instituto de Ciências da Saúde, Sinop, 2020.
http://ri.ufmt.br/handle/1/4843
identifier_str_mv MIRANDA, Ginislene Dias Souza. Efeito da desnutrição perinatal sobre o comportamento alimentar e função termogênica em ratos adultos. 2020. 74 f. Dissertação (Mestrado em Ciências em Saúde) - Universidade Federal de Mato Grosso, Campus Universitário de Sinop, Instituto de Ciências da Saúde, Sinop, 2020.
url http://ri.ufmt.br/handle/1/4843
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Mato Grosso
Brasil
Instituto de Ciências da Saúde (ICS) - Sinop
UFMT CUS - Sinop
Programa de Pós-Graduação em Ciências em Saúde
publisher.none.fl_str_mv Universidade Federal de Mato Grosso
Brasil
Instituto de Ciências da Saúde (ICS) - Sinop
UFMT CUS - Sinop
Programa de Pós-Graduação em Ciências em Saúde
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMT
instname:Universidade Federal de Mato Grosso (UFMT)
instacron:UFMT
instname_str Universidade Federal de Mato Grosso (UFMT)
instacron_str UFMT
institution UFMT
reponame_str Repositório Institucional da UFMT
collection Repositório Institucional da UFMT
repository.name.fl_str_mv Repositório Institucional da UFMT - Universidade Federal de Mato Grosso (UFMT)
repository.mail.fl_str_mv jordanbiblio@gmail.com
_version_ 1804648524162269184