Avaliação da atividade e do mecanismo de ação antibacteriana e antiúlcera do extrato hidroetanólico de Piper umbellatum L.
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFMT |
Texto Completo: | http://ri.ufmt.br/handle/1/3356 |
Resumo: | Piper umbellatum is a plant found in the Amazon region of Brazil and widely used in folk medicine of several countries for the treatment of gastric disorders and infections. The aim of this study was to evaluate the acute toxicity, antibacterial, preventive (gastroprotective) and curative (anti-ulcer) activites of hydroethanolic extract of the leaves of Piper umbellatum (HEPu), and still know the possible mechanisms involved in these activities. The leaves of Piper umbellatum were macerated in 75% hydroethanol solution (1:3, w/v) to obtain the HEPu. The acute toxicity of HEPu against CHO-K1 cells was evaluated by cytotoxicity assay with Alamar Blue and Hippocratic test using animals. The antibacterial activity was determined by the broth microdilution method using a panel of bacteria of clinical relevance and effects of HEPu the bacterial membrane was analyzed in detail. Preliminary phytochemical analysis based on coloration/precipitation were performed according to literature procedures. The secondary metabolites were analyzed and confirmed by thin layer chromatography (TLC) , spectrophotometry and high performance liquid chromatography (HPLC). The gastroprotective activity was assessed using the models of gastric ulcer acidified ethanol (acute) and acetic acid (chronic) in rodents Additionally, we evaluated the anti-Helicobacter pylori action of the extract. The probable mechanism of action of HEPu was also evaluated by determination of gastric secretory parameters, levels of antioxidant enzymes (catalase and glutathione) and malondialdehyde in gastric tissue, and proinflammatory cytokines (IL - 1β, TNF - α, IL -17, RANTES, IFN- γ and MIP-2 ) and antiinflammatory (IL -10). Piper umbellatum did not toxic in the in vitro cytotoxicity (IC50 > 200 µg/mL) test. When administered orally at doses up to 2000 mg/kg, HEPu did not cause any signs or symptoms of toxicity in mice. A good spectrum of antibacterial activity and mechanism of this action is associated with changes in the permeability of bacterial membranes was demonstrated. Preliminary phytochemical analysis revealed the presence of flavonoids, alkaloids, terpenes and steroids in extract. The flavonoids quercetin and rutin were detected in the spectrophotometric and HPLC analyzes. The results of antiulcer studies HEPu demonstrated potent gastroprotection against acute ulceration induced by acidified ethanol and excellent curative effect of chronic ulcers induced by acetic acid. The gastroprotection against acidified ethanol is partly attributable to the antioxidative mechanisms, while the anti-ulcer action contribute mechanisms antisecretory, antiinflammatory and regenerating the gastric mucosa. No part of this last action, the anti - Helicobacter pylori activity. The results of this study showed that the HEPu has preventive and curative effects on experimental models of gastric ulcers in animals. These effects are partially dependent antioxidants, antisecretory, anti- inflammatory and regenerative properties of the mucosa, and independent of anti-Helicobacter pylori activity, with the probable substances responsible for the pharmacological activity of flavonoids quercetin and rutin. These results confirm the popular use of the leaves of Piper umbellatum in the treatment of peptic ulcers and infections. |
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Avaliação da atividade e do mecanismo de ação antibacteriana e antiúlcera do extrato hidroetanólico de Piper umbellatum L.Piper umbellatumInfecçõesAntibacterianaÚlceras pépticasAntiúlceraCNPQ::CIENCIAS DA SAUDEPiper umbellatumInfectionsAntibacterialPeptic ulcersAntiulcerPiper umbellatum is a plant found in the Amazon region of Brazil and widely used in folk medicine of several countries for the treatment of gastric disorders and infections. The aim of this study was to evaluate the acute toxicity, antibacterial, preventive (gastroprotective) and curative (anti-ulcer) activites of hydroethanolic extract of the leaves of Piper umbellatum (HEPu), and still know the possible mechanisms involved in these activities. The leaves of Piper umbellatum were macerated in 75% hydroethanol solution (1:3, w/v) to obtain the HEPu. The acute toxicity of HEPu against CHO-K1 cells was evaluated by cytotoxicity assay with Alamar Blue and Hippocratic test using animals. The antibacterial activity was determined by the broth microdilution method using a panel of bacteria of clinical relevance and effects of HEPu the bacterial membrane was analyzed in detail. Preliminary phytochemical analysis based on coloration/precipitation were performed according to literature procedures. The secondary metabolites were analyzed and confirmed by thin layer chromatography (TLC) , spectrophotometry and high performance liquid chromatography (HPLC). The gastroprotective activity was assessed using the models of gastric ulcer acidified ethanol (acute) and acetic acid (chronic) in rodents Additionally, we evaluated the anti-Helicobacter pylori action of the extract. The probable mechanism of action of HEPu was also evaluated by determination of gastric secretory parameters, levels of antioxidant enzymes (catalase and glutathione) and malondialdehyde in gastric tissue, and proinflammatory cytokines (IL - 1β, TNF - α, IL -17, RANTES, IFN- γ and MIP-2 ) and antiinflammatory (IL -10). Piper umbellatum did not toxic in the in vitro cytotoxicity (IC50 > 200 µg/mL) test. When administered orally at doses up to 2000 mg/kg, HEPu did not cause any signs or symptoms of toxicity in mice. A good spectrum of antibacterial activity and mechanism of this action is associated with changes in the permeability of bacterial membranes was demonstrated. Preliminary phytochemical analysis revealed the presence of flavonoids, alkaloids, terpenes and steroids in extract. The flavonoids quercetin and rutin were detected in the spectrophotometric and HPLC analyzes. The results of antiulcer studies HEPu demonstrated potent gastroprotection against acute ulceration induced by acidified ethanol and excellent curative effect of chronic ulcers induced by acetic acid. The gastroprotection against acidified ethanol is partly attributable to the antioxidative mechanisms, while the anti-ulcer action contribute mechanisms antisecretory, antiinflammatory and regenerating the gastric mucosa. No part of this last action, the anti - Helicobacter pylori activity. The results of this study showed that the HEPu has preventive and curative effects on experimental models of gastric ulcers in animals. These effects are partially dependent antioxidants, antisecretory, anti- inflammatory and regenerative properties of the mucosa, and independent of anti-Helicobacter pylori activity, with the probable substances responsible for the pharmacological activity of flavonoids quercetin and rutin. These results confirm the popular use of the leaves of Piper umbellatum in the treatment of peptic ulcers and infections.CAPESCNPqPiper umbellatum é uma planta encontrada na região Amazônica do Brasil e amplamente empregada na medicina popular de vários países para o tratamento de distúrbios gástricos e infecções. O objetivo deste estudo foi avaliar a toxicidade aguda, as atividades antibacteriana, preventiva (gastroprotetora) e curativa (antiúlcera) do extrato hidroetanólico das folhas de Piper umbellatum (EHPu), e ainda conhecer os possíveis mecanismos de ação envolvidos nestas atividades. As folhas de Piper umbellatum foram maceradas em solução hidroetanólica 75% (1:3 p/v) para obter o HEPu. A toxicidade aguda do EHPu contra células CHO-K1 foi avaliada usando o ensaio de citotoxicidade com Alamar Blue e, em animais utilizando o teste Hipocrático. A atividade antibacteriana foi determinada através do método de microdiluição em caldo usando um painel de bactérias de relevância clínica e os efeitos do EHPu na membrana bacteriana foi analisado em detalhes. As análises fitoquímicas preliminares baseadas em coloração/precipitação foram realizadas de acordo a procedimentos descritos na literatura. Os metabólitos secundários foram analisados e confirmados por cromatografia em camada delgada (CCD), espectrofotometria e cromatografia líquida de alta performance (HPLC). A atividade gastroprotetora foi avaliada usando os modelos de úlcera gástrica por etanol acidificado (agudo) e ácido acético (crônico) em roedores. Adicionalmente avaliou-se a ação anti-Helicobacter pylori do extrato. O provável mecanismo de ação do EHPu foi também avaliado através da determinação de parâmetros secretórios gástricos, dos níveis de enzimas antioxidantes (catalase e glutationa) e malondialdeído em tecido gástrico, e das citocinas pró-inflamatórias (IL-1β, TNF-α, IL-17, RANTES, IFN-γ e MIP-2) e antiinflamatória (IL-10). Piper umbellatum não foi tóxico no teste de citotoxidade in vitro (CI50>200 µg/mL). Quando administrado oralmente in vivo em doses até 2000 mg/kg, EHPu não causou nenhum sinal ou sintomas de toxicidade nos camundongos. Foi demonstrado um bom espectro de atividade antibacteriana e o mecanismo dessa ação está associado com mudanças na permeabilidade das membranas bacterianas. As análises fitoquímicas preliminares revelaram a presença de flavonóides, alcalóides, terpenos e esteroides no extrato. Os flavonóides quercetina e rutina foram detectados nas análises de espectrofotometria e HPLC. Os resultados dos estudos antiúlcera de EHPu demonstraram potente gastroproteção contra a úlcera aguda induzida por etanol acidificado e excelente efeito curativo da úlcera crônica induzida pelo ácido acético. A gastroproteção contra o etanol acidificado é parcialmente atribuída a mecanismos antioxidantes, enquanto que na ação antiúlcera contribuem mecanismos anti-secretórios, anti-inflamatórios e regeneradores da mucosa gástrica. Não participa desta última ação, a atividade anti-Helicobacter pylori. Os resultados deste estudo mostraram que o EHPu possui efeitos preventivo e curativo em modelos experimentais de úlceras gástricas em animais. Esses efeitos são parcialmente dependentes de propriedades antioxidantes, anti-secretórios, anti-inflamatórios e regeneradores da mucosa, e independente da atividade anti-Helicobacter pylori, tendo como prováveis substâncias responsáveis pela atividade farmacológica os flavonóides quercetina e rutina. Esses resultados comprovam o uso popular das folhas de Piper umbellatum no tratamento de infecções e úlceras pépticas.Universidade Federal de Mato GrossoBrasilFaculdade de Medicina (FM)UFMT CUC - CuiabáPrograma de Pós-Graduação em Ciências da SaúdeMartins, Domingos Tabajara de Oliveirahttp://lattes.cnpq.br/3794477872946546Martins, Domingos Tabajara de Oliveira109.726.923-04http://lattes.cnpq.br/3794477872946546Lopes, Lousã879.675.298-04http://lattes.cnpq.br/8843725522665416109.726.923-04Violante, Ivana Maria Póvoa754.018.519-87http://lattes.cnpq.br/0296266156550935Rao, Vietla Satyanarayana210.583.503-15http://lattes.cnpq.br/7046546191056187Andrade, Sérgio Faloni de002.922.086-69http://lattes.cnpq.br/6811489046324705Silva Junior, Iberê Ferreira da2022-07-04T17:17:48Z2014-03-202022-07-04T17:17:48Z2014-02-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisSILVA JUNIOR, Iberê Ferreira da. Avaliação da atividade e do mecanismo de ação antibacteriana e antiúlcera do extrato hidroetanólico de Piper umbellatum L. 2014. 82 f. Tese (Doutorado em Ciências da Saúde) - Universidade Federal de Mato Grosso, Faculdade de Medicina, Cuiabá, 2014.http://ri.ufmt.br/handle/1/3356porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMTinstname:Universidade Federal de Mato Grosso (UFMT)instacron:UFMT2022-07-07T07:05:37Zoai:localhost:1/3356Repositório InstitucionalPUBhttp://ri.ufmt.br/oai/requestjordanbiblio@gmail.comopendoar:2022-07-07T07:05:37Repositório Institucional da UFMT - Universidade Federal de Mato Grosso (UFMT)false |
dc.title.none.fl_str_mv |
Avaliação da atividade e do mecanismo de ação antibacteriana e antiúlcera do extrato hidroetanólico de Piper umbellatum L. |
title |
Avaliação da atividade e do mecanismo de ação antibacteriana e antiúlcera do extrato hidroetanólico de Piper umbellatum L. |
spellingShingle |
Avaliação da atividade e do mecanismo de ação antibacteriana e antiúlcera do extrato hidroetanólico de Piper umbellatum L. Silva Junior, Iberê Ferreira da Piper umbellatum Infecções Antibacteriana Úlceras pépticas Antiúlcera CNPQ::CIENCIAS DA SAUDE Piper umbellatum Infections Antibacterial Peptic ulcers Antiulcer |
title_short |
Avaliação da atividade e do mecanismo de ação antibacteriana e antiúlcera do extrato hidroetanólico de Piper umbellatum L. |
title_full |
Avaliação da atividade e do mecanismo de ação antibacteriana e antiúlcera do extrato hidroetanólico de Piper umbellatum L. |
title_fullStr |
Avaliação da atividade e do mecanismo de ação antibacteriana e antiúlcera do extrato hidroetanólico de Piper umbellatum L. |
title_full_unstemmed |
Avaliação da atividade e do mecanismo de ação antibacteriana e antiúlcera do extrato hidroetanólico de Piper umbellatum L. |
title_sort |
Avaliação da atividade e do mecanismo de ação antibacteriana e antiúlcera do extrato hidroetanólico de Piper umbellatum L. |
author |
Silva Junior, Iberê Ferreira da |
author_facet |
Silva Junior, Iberê Ferreira da |
author_role |
author |
dc.contributor.none.fl_str_mv |
Martins, Domingos Tabajara de Oliveira http://lattes.cnpq.br/3794477872946546 Martins, Domingos Tabajara de Oliveira 109.726.923-04 http://lattes.cnpq.br/3794477872946546 Lopes, Lousã 879.675.298-04 http://lattes.cnpq.br/8843725522665416 109.726.923-04 Violante, Ivana Maria Póvoa 754.018.519-87 http://lattes.cnpq.br/0296266156550935 Rao, Vietla Satyanarayana 210.583.503-15 http://lattes.cnpq.br/7046546191056187 Andrade, Sérgio Faloni de 002.922.086-69 http://lattes.cnpq.br/6811489046324705 |
dc.contributor.author.fl_str_mv |
Silva Junior, Iberê Ferreira da |
dc.subject.por.fl_str_mv |
Piper umbellatum Infecções Antibacteriana Úlceras pépticas Antiúlcera CNPQ::CIENCIAS DA SAUDE Piper umbellatum Infections Antibacterial Peptic ulcers Antiulcer |
topic |
Piper umbellatum Infecções Antibacteriana Úlceras pépticas Antiúlcera CNPQ::CIENCIAS DA SAUDE Piper umbellatum Infections Antibacterial Peptic ulcers Antiulcer |
description |
Piper umbellatum is a plant found in the Amazon region of Brazil and widely used in folk medicine of several countries for the treatment of gastric disorders and infections. The aim of this study was to evaluate the acute toxicity, antibacterial, preventive (gastroprotective) and curative (anti-ulcer) activites of hydroethanolic extract of the leaves of Piper umbellatum (HEPu), and still know the possible mechanisms involved in these activities. The leaves of Piper umbellatum were macerated in 75% hydroethanol solution (1:3, w/v) to obtain the HEPu. The acute toxicity of HEPu against CHO-K1 cells was evaluated by cytotoxicity assay with Alamar Blue and Hippocratic test using animals. The antibacterial activity was determined by the broth microdilution method using a panel of bacteria of clinical relevance and effects of HEPu the bacterial membrane was analyzed in detail. Preliminary phytochemical analysis based on coloration/precipitation were performed according to literature procedures. The secondary metabolites were analyzed and confirmed by thin layer chromatography (TLC) , spectrophotometry and high performance liquid chromatography (HPLC). The gastroprotective activity was assessed using the models of gastric ulcer acidified ethanol (acute) and acetic acid (chronic) in rodents Additionally, we evaluated the anti-Helicobacter pylori action of the extract. The probable mechanism of action of HEPu was also evaluated by determination of gastric secretory parameters, levels of antioxidant enzymes (catalase and glutathione) and malondialdehyde in gastric tissue, and proinflammatory cytokines (IL - 1β, TNF - α, IL -17, RANTES, IFN- γ and MIP-2 ) and antiinflammatory (IL -10). Piper umbellatum did not toxic in the in vitro cytotoxicity (IC50 > 200 µg/mL) test. When administered orally at doses up to 2000 mg/kg, HEPu did not cause any signs or symptoms of toxicity in mice. A good spectrum of antibacterial activity and mechanism of this action is associated with changes in the permeability of bacterial membranes was demonstrated. Preliminary phytochemical analysis revealed the presence of flavonoids, alkaloids, terpenes and steroids in extract. The flavonoids quercetin and rutin were detected in the spectrophotometric and HPLC analyzes. The results of antiulcer studies HEPu demonstrated potent gastroprotection against acute ulceration induced by acidified ethanol and excellent curative effect of chronic ulcers induced by acetic acid. The gastroprotection against acidified ethanol is partly attributable to the antioxidative mechanisms, while the anti-ulcer action contribute mechanisms antisecretory, antiinflammatory and regenerating the gastric mucosa. No part of this last action, the anti - Helicobacter pylori activity. The results of this study showed that the HEPu has preventive and curative effects on experimental models of gastric ulcers in animals. These effects are partially dependent antioxidants, antisecretory, anti- inflammatory and regenerative properties of the mucosa, and independent of anti-Helicobacter pylori activity, with the probable substances responsible for the pharmacological activity of flavonoids quercetin and rutin. These results confirm the popular use of the leaves of Piper umbellatum in the treatment of peptic ulcers and infections. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-03-20 2014-02-28 2022-07-04T17:17:48Z 2022-07-04T17:17:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SILVA JUNIOR, Iberê Ferreira da. Avaliação da atividade e do mecanismo de ação antibacteriana e antiúlcera do extrato hidroetanólico de Piper umbellatum L. 2014. 82 f. Tese (Doutorado em Ciências da Saúde) - Universidade Federal de Mato Grosso, Faculdade de Medicina, Cuiabá, 2014. http://ri.ufmt.br/handle/1/3356 |
identifier_str_mv |
SILVA JUNIOR, Iberê Ferreira da. Avaliação da atividade e do mecanismo de ação antibacteriana e antiúlcera do extrato hidroetanólico de Piper umbellatum L. 2014. 82 f. Tese (Doutorado em Ciências da Saúde) - Universidade Federal de Mato Grosso, Faculdade de Medicina, Cuiabá, 2014. |
url |
http://ri.ufmt.br/handle/1/3356 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Mato Grosso Brasil Faculdade de Medicina (FM) UFMT CUC - Cuiabá Programa de Pós-Graduação em Ciências da Saúde |
publisher.none.fl_str_mv |
Universidade Federal de Mato Grosso Brasil Faculdade de Medicina (FM) UFMT CUC - Cuiabá Programa de Pós-Graduação em Ciências da Saúde |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFMT instname:Universidade Federal de Mato Grosso (UFMT) instacron:UFMT |
instname_str |
Universidade Federal de Mato Grosso (UFMT) |
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UFMT |
institution |
UFMT |
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Repositório Institucional da UFMT |
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Repositório Institucional da UFMT |
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Repositório Institucional da UFMT - Universidade Federal de Mato Grosso (UFMT) |
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jordanbiblio@gmail.com |
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1804648513180532736 |