Identificação de flavonoides por LC-MS/MS no extrato de folhas da Trattinnickia rhoifolia (Willd) e avaliação da atividade antioxidante

• Autor(a) principal: Magalhães, Lilian Marigo
• Data de Publicação: 2017
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFMT
• Texto Completo: http://ri.ufmt.br/handle/1/4169
• topic: Trattinnickia rhoifolia; Compostos fenólicos; Antioxidante; Hepatotoxicidade; CNPQ::OUTROS::CIENCIAS; Trattinnickia rhoifolia; Phenolic compounds; Antioxidant; Hepatotoxicity

Trattinickia rhoifolia is a medicinal plant popularly used to treat throat diseases, skin lacerations, treatment and prevention of tumors and leukemia. The study refers to the isolation of phenolic compounds, evaluation of the biological activity in mice and the sequestering capacity of free radicals in the extracts of the leaves of this plant. The leaves were collected in the municipality of Sinop / MT, dried in an oven, crushed and submitted to extraction of the chemical constituents with ethanol. The crude ethanolic extract was submitted to a partition that gave the hydromethanolic (HM) and ethyl acetate (EA) fractions. The chemical analyzes of total phenols were made by the Folin-Ciocalteau method, the total flavonoids by the aluminum chloride colorimetric method and the antioxidant potential performed by the DPPH• radical reduction method in an efficient concentration to reduce activity by 50% (EC 50). Flavonoids identified by liquid chromatography-mass spectrometry (LC-MS / MS). For biological analysis, male Swiss mice were obtained from the UFMT / Cuiabá Vivarium, treated with the HM and EA fractions. This research was certified according to protocol no. 23108.701484 / 14-2 of CEUA-UFMT. The groups were divided according to the type of treatment: GI-Control; GII-PCM (paracetamol); GIII-PCM + HM; GIV-PCM + EA; GV-HM; GVI-EA. Fractions (100 mg / kg) were given 7 days after 3 hours of paracetamol (250 mg / kg) induction of the lesion. Biochemical tests were performed and data were analyzed by ANOVA followed by Tukey (p <0.05). The assays determined the values for superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), reduced glutathione (GSH), carbonylated proteins (PC), ascorbic acid (ASA), thiobarbituric acid derivatives (TBARS) of oxidative stress in hepatic and / or renal tissues. Plasma parameters alanine aminotransferase (ALT), aspartate aminotransferase (AST), glycemia, triglycerides and cholesterol were evaluated. The results of total phenols were 436 ± 2.96 and 437 ± 6.05 mg EAG / g for HM and EA, respectively. For total flavonoids a content of 12 ± 0.18 and 40 ± 0.88 mg EQ / g in the HM and EA fractions, respectively, the EA fraction presented 35 μg / mL EC50 compared to the MH with 44 μg / mL EC50. In the crude ethanolic extract, Flavonoids: Amentoflavone, Apigenin, Canferol, Luteolin, Quercetin, Quercetin-3-β-Dglycoside, Rutin and Taxifoline were identified by LC-MS / MS using multiple reactions monitoring (MRM). Paracetamol decreased the activity of glutathione-S-transferase (GST), catalase (CAT) and alanine aminotransferase (ALT) and the concentrations of reduced glutathione (GSH) and ascorbic acid (ASA), besides increasing carbonylated proteins And lipid peroxidation (TBARS). These parameters returned to the levels of the control in the liver by treatments with PCM + HM and PCM + EA in CAT, ASA and PC, by PCM + HM treatment in TBARS and PCM + EA in GST. In the kidney the GSH and PC were restored by PCM + HM and PCM + EA treatments. Fractions also reduced plasma glucose and triglyceride levels. Based on the data presented, the EA fraction shows a greater capacity to revert alterations provoked in the biochemical parameters analyzed because it presents concentration of flavonoids and greater antioxidant activity in comparison to the HM.