ATIVIDADE ANTIMICROBIANA E ANTIBIOFILME DE NANOPARTÍCULAS DE ÓLEO DE Melaleuca alternifolia

• Autor(a) principal: Souza, Márcia Ebling de
• Data de Publicação: 2014
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional Universidade Franciscana
• Texto Completo: http://tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/217; http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/327
• topic: Candida; Resistência; Alternativa terapêutica; Nanotecnologia; Árvore do Chá; Candida; Resistance; Alternative therapy; Nanotechnology; Tea Tree; CNPQ::ENGENHARIAS

Infections caused by Candida species are an important cause of morbidity and mortality in immunocompromised patients. This growing trend has been linked to resistance to antimicrobial therapy and the ability of the organism to form biofilms. Melaleuca alternifolia oil is traditionally used as an herbal antimicrobial that shows activity in biofilms formed by Candida species. The present study aimed to evaluate the antimicrobial and antibiofilm activity nanoparticles and the essential oil of M. alternifolia. Oil and characterization of nanoparticles M. alternifolia. Antimicrobial activity was determined by the microdilution technique was performed. The ability to form biofilms at different glucose concentrations and measured the levels of exopolysaccharides and proteins in the biofilms was verified. The qualitative analysis of biofilms was performed by staining of Calcofluor White. The cytotoxicity of nanoparticles and the oil was assessed. The chromatographic profile showed that M. alternifolia oil conforms with ISO 4730 with the main constituents, 41.9% of Terpinen-4-ol, 20.1% of γ-terpinene, 9.8% of α-terpinene and 6.0% of 1.8-Cineol. Nanoparticles M. alternifolia oil had a pH of 6.3, particle diameter of 158.2 ± 2 nm, polydispersity 0.213 ± 0.017 and zeta potential of -8.69 ± 0.80 mV. The oil and nanoparticles M. alternifolia showed antimicrobial activity against most microorganisms evaluated. Microorganisms that showed an increased capacity to form biofilms were C. albicans, C. tropicalis, C. membranafaciens, C. parapsilosis and C. glabrata at a concentration of 1% glucose. Administration of oil or nanoparticles of M. alternifolia oil represented a significant reduction of biofilm formed by all Candida species used, as well as a reduction in protein levels and exopolysaccharide biofilms. Was possible to visualize the reduction of biofilm in the presence of nanoparticles of M. alternifolia with Calcofluor White staining. The oil and nanoparticles M. alternifolia showed cytotoxic character at all concentrations evaluated. With the increase in the concentration of fetal calf serum medium, an inhibition of the toxic effect of the nanoparticles occurs. There are probably involved in protein-corona effect on the cytotoxicity of nanoparticles