AVALIAÇÃO TOXICOLÓGICA DE NANOPARTÍCULAS LIPÍDICAS SÓLIDAS DE CURCUMINA E CAPSAICINA E SEU EFEITO SOBRE A COLITE ULCERATIVA INDUZIDA QUIMICAMENTE EM CAMUNDONGOS C57BL/6

Detalhes bibliográficos
Autor(a) principal: Martins, Nára Maria Beck
Data de Publicação: 2023
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional Universidade Franciscana
Texto Completo: http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/1203
Resumo: Idiopathic Inflammatory Bowel Disease (IBD) is a chronic inflammatory disorder of the gastrointestinal tract related to the exacerbation of the immune response. The two most well-known types are Crohn's disease (CD) and ulcerative intestinal colitis (UC), with increasing incidence worldwide. The traditional treatment of this type of illness involves aminosalicylates, corticosteroids, and immunosuppressants, all with significant side effects and toxicity. To try to reduce the side effects of these medications, one of the strategies is using spices traditionally used in cooking that demonstrate an anti-inflammatory effect in current research. Curcumin, a polyphenol, and capsaicin, an alkaloid, stand out in publications for their anti-inflammatory power. These substances have low solubility in water and low bioavailability, so encapsulation in a nanostructure is a strategy for applicability in a biological environment. The objectives of this work were to evaluate: i) toxicological parameters of solid lipid nanoparticles of curcumin and capsaicin (NCC) and its actives, and ii) the anti-inflammatory effect of NCC in mice submitted to ulcerative colitis (UC) induced by dextran sulfate sodium (DSS). First, was evaluated the safety profile of NCC (3 mg/kg of curcumin and 0.1 mg/kg of capsaicin), active free nanoparticles called white nanoparticles (NB), and unencapsulated curcumin and capsaicin (CC) in vivo by oral administration for 7 days in twenty-four female C57BL/6 mice and hematological analyses, liver and kidney biochemical markers and histopathological changes were performed. The biochemical and histopathological parameters of the metabolizing and excreting organs did not indicate significant alterations, showing that, under the conditions of this study, CC, NB, and NCC are biocompatible and do not cause systemic toxicity. In the second part of the work, thirty-two female C57BL/6 mice were subjected to experimental UC by adding 5% DSS to their water bottles. Mice were divided into four groups: Vehicle (VE), UC (DSS 5%), UC plus sulfasalazine (UC+S, 50 mg/kg), and UC plus NCC. Mice were treated with sulfasalazine or NCC by gavage for seven days. After treatment, the animals were evaluated for pyruvate kinase (PK) activity and expression, TNF-α levels, and histopathological parameters. Results showed that UC induced inflammation in the colon, and sulfasalazine effectively reduced tissue damage. However, NCC did not have the same protective effect as sulfasalazine. UC also affected PK activity in the colon and brain, and NCC only protected against the effect of UC on brain PK activity. The study concluded that the nanostructures practically did not change the toxicological parameters, the changes in PK activity induced by UC seem to be associated with PKM2 expression, and sulfasalazine was more effective than NCC in protecting against histopathological changes in UC.
id UFN-1_793c55db38de690515c7d5611ef6b026
oai_identifier_str oai:tede.universidadefranciscana.edu.br:UFN-BDTD/1203
network_acronym_str UFN-1
network_name_str Repositório Institucional Universidade Franciscana
repository_id_str http://www.tede.universidadefranciscana.edu.br:8080/oai/request
spelling Rech , Virginia CieloMoraes , Jefferson PotiguaraSegat , Hecson JesserFernandes, Liana da SilvaVizzotto, Bruno StefanelloMartins, Nára Maria Beck2023-09-15T14:05:48Z2023-07-14Martins, Nára Maria Beck. AVALIAÇÃO TOXICOLÓGICA DE NANOPARTÍCULAS LIPÍDICAS SÓLIDAS DE CURCUMINA E CAPSAICINA E SEU EFEITO SOBRE A COLITE ULCERATIVA INDUZIDA QUIMICAMENTE EM CAMUNDONGOS C57BL/6. 2023. 77f. Tese( Programa de Pós-Graduação em Nanociências) - Universidade Franciscana, Santa Maria - RS.http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/1203Idiopathic Inflammatory Bowel Disease (IBD) is a chronic inflammatory disorder of the gastrointestinal tract related to the exacerbation of the immune response. The two most well-known types are Crohn's disease (CD) and ulcerative intestinal colitis (UC), with increasing incidence worldwide. The traditional treatment of this type of illness involves aminosalicylates, corticosteroids, and immunosuppressants, all with significant side effects and toxicity. To try to reduce the side effects of these medications, one of the strategies is using spices traditionally used in cooking that demonstrate an anti-inflammatory effect in current research. Curcumin, a polyphenol, and capsaicin, an alkaloid, stand out in publications for their anti-inflammatory power. These substances have low solubility in water and low bioavailability, so encapsulation in a nanostructure is a strategy for applicability in a biological environment. The objectives of this work were to evaluate: i) toxicological parameters of solid lipid nanoparticles of curcumin and capsaicin (NCC) and its actives, and ii) the anti-inflammatory effect of NCC in mice submitted to ulcerative colitis (UC) induced by dextran sulfate sodium (DSS). First, was evaluated the safety profile of NCC (3 mg/kg of curcumin and 0.1 mg/kg of capsaicin), active free nanoparticles called white nanoparticles (NB), and unencapsulated curcumin and capsaicin (CC) in vivo by oral administration for 7 days in twenty-four female C57BL/6 mice and hematological analyses, liver and kidney biochemical markers and histopathological changes were performed. The biochemical and histopathological parameters of the metabolizing and excreting organs did not indicate significant alterations, showing that, under the conditions of this study, CC, NB, and NCC are biocompatible and do not cause systemic toxicity. In the second part of the work, thirty-two female C57BL/6 mice were subjected to experimental UC by adding 5% DSS to their water bottles. Mice were divided into four groups: Vehicle (VE), UC (DSS 5%), UC plus sulfasalazine (UC+S, 50 mg/kg), and UC plus NCC. Mice were treated with sulfasalazine or NCC by gavage for seven days. After treatment, the animals were evaluated for pyruvate kinase (PK) activity and expression, TNF-α levels, and histopathological parameters. Results showed that UC induced inflammation in the colon, and sulfasalazine effectively reduced tissue damage. However, NCC did not have the same protective effect as sulfasalazine. UC also affected PK activity in the colon and brain, and NCC only protected against the effect of UC on brain PK activity. The study concluded that the nanostructures practically did not change the toxicological parameters, the changes in PK activity induced by UC seem to be associated with PKM2 expression, and sulfasalazine was more effective than NCC in protecting against histopathological changes in UC.A Doença Inflamatória Intestinal Idiopática (DII) é um distúrbio inflamatório crônico do trato gastrointestinal relacionado à exacerbação da resposta imune. Os dois tipos mais conhecidos são a doença de Crohn (DC) e a colite intestinal ulcerativa (UC), com incidência crescente em todo o mundo. O tratamento tradicional desse tipo de doença envolve aminossalicilatos, corticosteroides e imunossupressores, todos com importantes efeitos colaterais e toxicidade. Para tentar diminuir os efeitos colaterais desses medicamentos, uma das estratégias é o uso de temperos tradicionalmente utilizados na culinária que demonstram efeito anti-inflamatório em pesquisas atuais. A curcumina, um polifenol, e a capsaicina, um alcaloide, destacam-se nas publicações por seu poder anti-inflamatório. Essas substâncias possuem baixa solubilidade em água e baixa biodisponibilidade, portanto o encapsulamento em uma nanoestrutura é uma estratégia que viabiliza a sua utilização em meio biológico. Os objetivos deste trabalho foram avaliar: i) parâmetros toxicológicos de nanopartículas lipídicas sólidas de curcumina e capsaicina (NCC) e comparar aos seus ativos livres, e ii) o efeito anti-inflamatório de NCC em camundongos submetidos à colite ulcerativa (CU) induzida por dextran sulfato de sódio (DSS). Na primeira parte do trabalho, foi avaliado o perfil de segurança in vivo de NCC (3 mg/kg de curcumina e 0,1 mg/kg de capsaicina); nanopartículas livres de ativos chamadas de nanopartículas brancas (NB); e curcumina e capsaicina não encapsuladas (CC), através da administração oral das substâncias durante 7 dias em vinte e quatro camundongos fêmeas C57BL/6 e realizadas análises hematológicas, marcadores bioquímicos hepáticos e renais e alterações histopatológicas. Os parâmetros hematológicos, bioquímicos e histopatológicos dos órgãos metabolizadores e excretores não indicaram alterações significativas, mostrando que, nas condições deste estudo, CC, NB e NCC são biocompatíveis e não causam toxicidade sistêmica. Na segunda parte do trabalho, trinta e dois camundongos fêmeas C57BL/6 foram submetidos a CU experimental adicionando 5% de DSS às suas garrafas de água. Os camundongos foram divididos em quatro grupos: Veículo (VE), CU (DSS 5%), CU mais sulfasalazina (CU+S, 50 mg/kg) e CU mais NCC. Os camundongos foram tratados com sulfasalazina ou NCC por gavagem, por sete dias. Após o tratamento, os animais foram avaliados quanto à atividade e expressão da piruvato cinase (PK), níveis de TNF-α e parâmetros histopatológicos. Os resultados mostraram que a CU induziu inflamação no cólon e a sulfasalazina reduziu efetivamente o dano tecidual. No entanto, o NCC não teve o mesmo efeito protetor que a sulfasalazina. A CU também afetou a atividade PK no cólon e no cérebro, e o NCC apenas protegeu contra o efeito da CU na atividade PK cerebral. O estudo concluiu que as nanoestruturas praticamente não alteraram os parâmetros toxicológicos, as alterações na atividade de PK induzidas por CU parecem estar associadas à expressão de PKM2, e a sulfassalazina foi mais eficaz que NCC na proteção contra alterações histopatológicas em CU.Submitted by Clarice Rosa Machado (clarice.machado@ufn.edu.br) on 2023-09-15T14:05:48Z No. of bitstreams: 2 Tese_NaraMariaBeckMartinsTEDE.pdf: 3615403 bytes, checksum: d100b8efbc4e285bc5f3ffefb8e6cc7d (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2023-09-15T14:05:48Z (GMT). No. of bitstreams: 2 Tese_NaraMariaBeckMartinsTEDE.pdf: 3615403 bytes, checksum: d100b8efbc4e285bc5f3ffefb8e6cc7d (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2023-07-14application/pdfhttp://www.tede.universidadefranciscana.edu.br:8080/retrieve/10924/Tese_NaraMariaBeckMartinsTEDE.pdf.jpgporUniversidade FranciscanaPrograma de Pós-Graduação em NanociênciasUFNBrasilBiociências e Nanomateriaishttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessInflamação. Histopatologia. Toxicidade. Nanotoxicologia. Metabolismo cerebral.Inflammation. Histopathology; Toxicity. Nanotoxicology. Brain metabolism.InterdisciplinarAVALIAÇÃO TOXICOLÓGICA DE NANOPARTÍCULAS LIPÍDICAS SÓLIDAS DE CURCUMINA E CAPSAICINA E SEU EFEITO SOBRE A COLITE ULCERATIVA INDUZIDA QUIMICAMENTE EM CAMUNDONGOS C57BL/6info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisreponame:Repositório Institucional Universidade Franciscanainstname:Universidade Franciscana (UFN)instacron:UFNTHUMBNAILTese_NaraMariaBeckMartinsTEDE.pdf.jpgTese_NaraMariaBeckMartinsTEDE.pdf.jpgimage/jpeg4068http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/1203/7/Tese_NaraMariaBeckMartinsTEDE.pdf.jpgbe5684fc269c34a0e410c74610d5fc75MD57TEXTTese_NaraMariaBeckMartinsTEDE.pdf.txtTese_NaraMariaBeckMartinsTEDE.pdf.txttext/plain132395http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/1203/6/Tese_NaraMariaBeckMartinsTEDE.pdf.txt38de1145a3bdeceeb7b86adb9ee2a9faMD56ORIGINALTese_NaraMariaBeckMartinsTEDE.pdfTese_NaraMariaBeckMartinsTEDE.pdfapplication/pdf3615403http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/1203/5/Tese_NaraMariaBeckMartinsTEDE.pdfd100b8efbc4e285bc5f3ffefb8e6cc7dMD55CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/1203/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/1203/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/1203/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-8310http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/1203/1/license.txt7dc66ddb96829ff34b56b1a92c851bcdMD51UFN-BDTD/12032023-09-16 01:00:10.205oai:tede.universidadefranciscana.edu.br:UFN-BDTD/1203RXN0ZSB0cmFiYWxobyBzZXLDoSBsaWNlbmNpYWRvIHNvYiBhIExpY2Vuw6dhIEF0cmlidWnDp8Ojby1Ow6NvQ29tZXJjaWFsLVNlbURlcml2YcOnw7VlcyA0LjAgSW50ZXJuYWNpb25hbCBDcmVhdGl2ZSBDb21tb25zLiBQYXJhIHZpc3VhbGl6YXIgdW1hIGPDs3BpYSBkZXN0YSBsaWNlbsOnYSwgdmlzaXRlIGh0dHA6Ly9jcmVhdGl2ZWNvbW1vbnMub3JnL2xpY2Vuc2VzL2J5LW5jLW5kLzQuMC8gb3UgbWFuZGUgdW1hIGNhcnRhIHBhcmEgQ3JlYXRpdmUgQ29tbW9ucywgUE8gQm94IDE4NjYsIE1vdW50YWluIFZpZXcsIENBIDk0MDQyLCBVU0EuCg==Repositório de Publicaçõeshttp://www.tede.universidadefranciscana.edu.br:8080/http://www.tede.universidadefranciscana.edu.br:8080/oai/requestopendoar:2023-09-16T04:00:10Repositório Institucional Universidade Franciscana - Universidade Franciscana (UFN)false
dc.title.por.fl_str_mv AVALIAÇÃO TOXICOLÓGICA DE NANOPARTÍCULAS LIPÍDICAS SÓLIDAS DE CURCUMINA E CAPSAICINA E SEU EFEITO SOBRE A COLITE ULCERATIVA INDUZIDA QUIMICAMENTE EM CAMUNDONGOS C57BL/6
title AVALIAÇÃO TOXICOLÓGICA DE NANOPARTÍCULAS LIPÍDICAS SÓLIDAS DE CURCUMINA E CAPSAICINA E SEU EFEITO SOBRE A COLITE ULCERATIVA INDUZIDA QUIMICAMENTE EM CAMUNDONGOS C57BL/6
spellingShingle AVALIAÇÃO TOXICOLÓGICA DE NANOPARTÍCULAS LIPÍDICAS SÓLIDAS DE CURCUMINA E CAPSAICINA E SEU EFEITO SOBRE A COLITE ULCERATIVA INDUZIDA QUIMICAMENTE EM CAMUNDONGOS C57BL/6
Martins, Nára Maria Beck
Inflamação. Histopatologia. Toxicidade. Nanotoxicologia. Metabolismo cerebral.
Inflammation. Histopathology; Toxicity. Nanotoxicology. Brain metabolism.
Interdisciplinar
title_short AVALIAÇÃO TOXICOLÓGICA DE NANOPARTÍCULAS LIPÍDICAS SÓLIDAS DE CURCUMINA E CAPSAICINA E SEU EFEITO SOBRE A COLITE ULCERATIVA INDUZIDA QUIMICAMENTE EM CAMUNDONGOS C57BL/6
title_full AVALIAÇÃO TOXICOLÓGICA DE NANOPARTÍCULAS LIPÍDICAS SÓLIDAS DE CURCUMINA E CAPSAICINA E SEU EFEITO SOBRE A COLITE ULCERATIVA INDUZIDA QUIMICAMENTE EM CAMUNDONGOS C57BL/6
title_fullStr AVALIAÇÃO TOXICOLÓGICA DE NANOPARTÍCULAS LIPÍDICAS SÓLIDAS DE CURCUMINA E CAPSAICINA E SEU EFEITO SOBRE A COLITE ULCERATIVA INDUZIDA QUIMICAMENTE EM CAMUNDONGOS C57BL/6
title_full_unstemmed AVALIAÇÃO TOXICOLÓGICA DE NANOPARTÍCULAS LIPÍDICAS SÓLIDAS DE CURCUMINA E CAPSAICINA E SEU EFEITO SOBRE A COLITE ULCERATIVA INDUZIDA QUIMICAMENTE EM CAMUNDONGOS C57BL/6
title_sort AVALIAÇÃO TOXICOLÓGICA DE NANOPARTÍCULAS LIPÍDICAS SÓLIDAS DE CURCUMINA E CAPSAICINA E SEU EFEITO SOBRE A COLITE ULCERATIVA INDUZIDA QUIMICAMENTE EM CAMUNDONGOS C57BL/6
author Martins, Nára Maria Beck
author_facet Martins, Nára Maria Beck
author_role author
dc.contributor.advisor1.fl_str_mv Rech , Virginia Cielo
dc.contributor.referee1.fl_str_mv Moraes , Jefferson Potiguara
dc.contributor.referee2.fl_str_mv Segat , Hecson Jesser
dc.contributor.referee3.fl_str_mv Fernandes, Liana da Silva
dc.contributor.referee4.fl_str_mv Vizzotto, Bruno Stefanello
dc.contributor.author.fl_str_mv Martins, Nára Maria Beck
contributor_str_mv Rech , Virginia Cielo
Moraes , Jefferson Potiguara
Segat , Hecson Jesser
Fernandes, Liana da Silva
Vizzotto, Bruno Stefanello
dc.subject.por.fl_str_mv Inflamação. Histopatologia. Toxicidade. Nanotoxicologia. Metabolismo cerebral.
topic Inflamação. Histopatologia. Toxicidade. Nanotoxicologia. Metabolismo cerebral.
Inflammation. Histopathology; Toxicity. Nanotoxicology. Brain metabolism.
Interdisciplinar
dc.subject.eng.fl_str_mv Inflammation. Histopathology; Toxicity. Nanotoxicology. Brain metabolism.
dc.subject.cnpq.fl_str_mv Interdisciplinar
description Idiopathic Inflammatory Bowel Disease (IBD) is a chronic inflammatory disorder of the gastrointestinal tract related to the exacerbation of the immune response. The two most well-known types are Crohn's disease (CD) and ulcerative intestinal colitis (UC), with increasing incidence worldwide. The traditional treatment of this type of illness involves aminosalicylates, corticosteroids, and immunosuppressants, all with significant side effects and toxicity. To try to reduce the side effects of these medications, one of the strategies is using spices traditionally used in cooking that demonstrate an anti-inflammatory effect in current research. Curcumin, a polyphenol, and capsaicin, an alkaloid, stand out in publications for their anti-inflammatory power. These substances have low solubility in water and low bioavailability, so encapsulation in a nanostructure is a strategy for applicability in a biological environment. The objectives of this work were to evaluate: i) toxicological parameters of solid lipid nanoparticles of curcumin and capsaicin (NCC) and its actives, and ii) the anti-inflammatory effect of NCC in mice submitted to ulcerative colitis (UC) induced by dextran sulfate sodium (DSS). First, was evaluated the safety profile of NCC (3 mg/kg of curcumin and 0.1 mg/kg of capsaicin), active free nanoparticles called white nanoparticles (NB), and unencapsulated curcumin and capsaicin (CC) in vivo by oral administration for 7 days in twenty-four female C57BL/6 mice and hematological analyses, liver and kidney biochemical markers and histopathological changes were performed. The biochemical and histopathological parameters of the metabolizing and excreting organs did not indicate significant alterations, showing that, under the conditions of this study, CC, NB, and NCC are biocompatible and do not cause systemic toxicity. In the second part of the work, thirty-two female C57BL/6 mice were subjected to experimental UC by adding 5% DSS to their water bottles. Mice were divided into four groups: Vehicle (VE), UC (DSS 5%), UC plus sulfasalazine (UC+S, 50 mg/kg), and UC plus NCC. Mice were treated with sulfasalazine or NCC by gavage for seven days. After treatment, the animals were evaluated for pyruvate kinase (PK) activity and expression, TNF-α levels, and histopathological parameters. Results showed that UC induced inflammation in the colon, and sulfasalazine effectively reduced tissue damage. However, NCC did not have the same protective effect as sulfasalazine. UC also affected PK activity in the colon and brain, and NCC only protected against the effect of UC on brain PK activity. The study concluded that the nanostructures practically did not change the toxicological parameters, the changes in PK activity induced by UC seem to be associated with PKM2 expression, and sulfasalazine was more effective than NCC in protecting against histopathological changes in UC.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-09-15T14:05:48Z
dc.date.issued.fl_str_mv 2023-07-14
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv Martins, Nára Maria Beck. AVALIAÇÃO TOXICOLÓGICA DE NANOPARTÍCULAS LIPÍDICAS SÓLIDAS DE CURCUMINA E CAPSAICINA E SEU EFEITO SOBRE A COLITE ULCERATIVA INDUZIDA QUIMICAMENTE EM CAMUNDONGOS C57BL/6. 2023. 77f. Tese( Programa de Pós-Graduação em Nanociências) - Universidade Franciscana, Santa Maria - RS.
dc.identifier.uri.fl_str_mv http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/1203
identifier_str_mv Martins, Nára Maria Beck. AVALIAÇÃO TOXICOLÓGICA DE NANOPARTÍCULAS LIPÍDICAS SÓLIDAS DE CURCUMINA E CAPSAICINA E SEU EFEITO SOBRE A COLITE ULCERATIVA INDUZIDA QUIMICAMENTE EM CAMUNDONGOS C57BL/6. 2023. 77f. Tese( Programa de Pós-Graduação em Nanociências) - Universidade Franciscana, Santa Maria - RS.
url http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/1203
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Franciscana
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Nanociências
dc.publisher.initials.fl_str_mv UFN
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Biociências e Nanomateriais
publisher.none.fl_str_mv Universidade Franciscana
dc.source.none.fl_str_mv reponame:Repositório Institucional Universidade Franciscana
instname:Universidade Franciscana (UFN)
instacron:UFN
instname_str Universidade Franciscana (UFN)
instacron_str UFN
institution UFN
reponame_str Repositório Institucional Universidade Franciscana
collection Repositório Institucional Universidade Franciscana
bitstream.url.fl_str_mv http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/1203/7/Tese_NaraMariaBeckMartinsTEDE.pdf.jpg
http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/1203/6/Tese_NaraMariaBeckMartinsTEDE.pdf.txt
http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/1203/5/Tese_NaraMariaBeckMartinsTEDE.pdf
http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/1203/2/license_url
http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/1203/3/license_text
http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/1203/4/license_rdf
http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/1203/1/license.txt
bitstream.checksum.fl_str_mv be5684fc269c34a0e410c74610d5fc75
38de1145a3bdeceeb7b86adb9ee2a9fa
d100b8efbc4e285bc5f3ffefb8e6cc7d
4afdbb8c545fd630ea7db775da747b2f
d41d8cd98f00b204e9800998ecf8427e
d41d8cd98f00b204e9800998ecf8427e
7dc66ddb96829ff34b56b1a92c851bcd
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional Universidade Franciscana - Universidade Franciscana (UFN)
repository.mail.fl_str_mv
_version_ 1809269407928549376