TOXICIDADE DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM MACRÓFAGOS
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Universidade Franciscana |
Texto Completo: | http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/536 |
Resumo: | Carbon nanotubes (CNT) have unique properties that are of great interest to the biotechnology area. The idea of using CNTs as carriers of drugs, adjuvants for vaccines and biosensors, is attracting great interest from the scientific community. However, the biocompatibility and toxicity in vitro and in vivo of these structures need to be investigated. Thus, the aim of this study was to investigate the cytotoxicity caused by carbon nanotubes multi-walled (MWCNT) in peritoneal macrophages isolated from C57BL/6 mice and the secondary line RAW 264.7. To perform the in vitro test, cultured cells were treated with concentrations of 1 mg/mL and 5mg/mL MWCNT and 1μg/mL of lipopolysaccharide (LPS). The control group of cells with culture medium were cultured in DMEM medium supplemented with 10% fetal bovine serum (FBS) and the negative control at 100 mϻ hydrogen peroxide. The cells remained in treatments for periods of 24 and 48 hours. Then was determined cell viability through the exclusion method using trypan blue, which remained around 70%. After the biochemical cytotoxicity tests were conducted using the release of lactate dehydrogenase (LDH) in the cell supernatant, allowing to note that the concentrations of MWCNT were unable to cause any damage to the cell membrane, as well as any cytotoxic effect. These results confirm the microscopic images, which do not show cell death caused by MWCNT at these concentrations. The citotoxicity caused by MWCNT was evaluated by the measurement of nitric oxide (NO) and the gene expression of TNF-α and IL-1β. In these tests it was revealed that the toxicity was present in cells treated with the secondary line MWCNT, which did not have an increase in NO production and gene expression of cytokines analyzed. |
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Hoelzel, Solange Cristina da Silva MartinsKrause, Luciana Maria FontanariRomão, Pedro Roosvelt TorresRossato, JussaneMüller, Betina2018-08-16T19:45:32Z2015-03-23Müller, Betina. TOXICIDADE DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM MACRÓFAGOS. 2015. 78f. Dissertação( Programa de Pós-Graduação em Nanociências) - Centro Universitário Franciscano, Santa Maria - RS .http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/536Carbon nanotubes (CNT) have unique properties that are of great interest to the biotechnology area. The idea of using CNTs as carriers of drugs, adjuvants for vaccines and biosensors, is attracting great interest from the scientific community. However, the biocompatibility and toxicity in vitro and in vivo of these structures need to be investigated. Thus, the aim of this study was to investigate the cytotoxicity caused by carbon nanotubes multi-walled (MWCNT) in peritoneal macrophages isolated from C57BL/6 mice and the secondary line RAW 264.7. To perform the in vitro test, cultured cells were treated with concentrations of 1 mg/mL and 5mg/mL MWCNT and 1μg/mL of lipopolysaccharide (LPS). The control group of cells with culture medium were cultured in DMEM medium supplemented with 10% fetal bovine serum (FBS) and the negative control at 100 mϻ hydrogen peroxide. The cells remained in treatments for periods of 24 and 48 hours. Then was determined cell viability through the exclusion method using trypan blue, which remained around 70%. After the biochemical cytotoxicity tests were conducted using the release of lactate dehydrogenase (LDH) in the cell supernatant, allowing to note that the concentrations of MWCNT were unable to cause any damage to the cell membrane, as well as any cytotoxic effect. These results confirm the microscopic images, which do not show cell death caused by MWCNT at these concentrations. The citotoxicity caused by MWCNT was evaluated by the measurement of nitric oxide (NO) and the gene expression of TNF-α and IL-1β. In these tests it was revealed that the toxicity was present in cells treated with the secondary line MWCNT, which did not have an increase in NO production and gene expression of cytokines analyzed.Os nanotubos de carbono (NTC) apresentam propriedades exclusivas que são de grande interesse para a área de biotecnologia. A idéia de se utilizar NTCs como carreadores de fármacos, adjuvantes para vacinas e biosensores, vem despertando grande interesse da comunidade científica. No entanto, a biocompatibilidade e toxicidade in vitro e in vivo dessas estruturas precisam ser investigadas. Com isso, o objetivo desse trabalho foi investigar a citotoxicidade causada pelos nanotubos de carbono de paredes múltipla (NTCPM) em macrófagos peritoneais isolados de camundongos C57BL/6, e macrófagos da linhagem secundária RAW 264.7. Para a realização dos testes in vitro, as células tratadas foram cultivadas com as concentrações de 1mg/mL e 5mg/mL de NTCPM, e 1μg/mL de lipopolissacarídeo (LPS). As células do grupo controle com meio de cultura foram cultivadas com meio de cultura DMEM suplementado a 10% de soro bovino fetal (SBF), e o controle negativo com 100 mϻ de peróxido de hidrogênio. As células permaneceram em tratamentos pelos períodos de 24 e 48 horas. Em seguida foi determinado a viabilidade celular, através do método de exclusão pelo azul tripan, a qual permaneceu em torno de 70%. Após foram conduzidos os testes bioquímicos de citotoxicidade através da liberação da enzima lactato desidrogenase (LDH) no sobrenadante celular, o que possibilitou observar que as concentrações dos NTCPM utilizadas não foram capazes de causar nenhum dano na membrana celular, assim como nenhum efeito citotóxico. Estes resultados confirmam as imagens microscópicas, as quais não mostram a morte celular causada pelos NTCPM nestas concentrações. A toxicidade causada pelos NTCPM foi avaliada através da dosagem do óxido nítrico (NO) e pela expressão gênica das citocinas TNF-α e IL-1β. Nesses testes foi possível perceber que a citotoxicidade não se fez presente nas células da linhagem secundária tratadas com NTCPM, as quais não tiveram um aumento da produção de NO e na expressão gênica das citocinas analisadas.Submitted by MARCIA ROVADOSCHI (marciar@unifra.br) on 2018-08-16T19:45:32Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Dissertacao_BetinaMuller.pdf: 2340597 bytes, checksum: 9b2e7abafe20d9c76ebb8713b0789f07 (MD5)Made available in DSpace on 2018-08-16T19:45:32Z (GMT). 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dc.title.por.fl_str_mv |
TOXICIDADE DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM MACRÓFAGOS |
title |
TOXICIDADE DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM MACRÓFAGOS |
spellingShingle |
TOXICIDADE DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM MACRÓFAGOS Müller, Betina Nanotubos de Carbono. Macrófagos. Citotoxicidade. Citocinas Carbon nanotubes. Macrophages. Citotoxicity. Cytokines Biociências e Nanomateriais |
title_short |
TOXICIDADE DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM MACRÓFAGOS |
title_full |
TOXICIDADE DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM MACRÓFAGOS |
title_fullStr |
TOXICIDADE DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM MACRÓFAGOS |
title_full_unstemmed |
TOXICIDADE DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM MACRÓFAGOS |
title_sort |
TOXICIDADE DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM MACRÓFAGOS |
author |
Müller, Betina |
author_facet |
Müller, Betina |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Hoelzel, Solange Cristina da Silva Martins |
dc.contributor.advisor-co1.fl_str_mv |
Krause, Luciana Maria Fontanari |
dc.contributor.referee1.fl_str_mv |
Romão, Pedro Roosvelt Torres |
dc.contributor.referee2.fl_str_mv |
Rossato, Jussane |
dc.contributor.author.fl_str_mv |
Müller, Betina |
contributor_str_mv |
Hoelzel, Solange Cristina da Silva Martins Krause, Luciana Maria Fontanari Romão, Pedro Roosvelt Torres Rossato, Jussane |
dc.subject.por.fl_str_mv |
Nanotubos de Carbono. Macrófagos. Citotoxicidade. Citocinas |
topic |
Nanotubos de Carbono. Macrófagos. Citotoxicidade. Citocinas Carbon nanotubes. Macrophages. Citotoxicity. Cytokines Biociências e Nanomateriais |
dc.subject.eng.fl_str_mv |
Carbon nanotubes. Macrophages. Citotoxicity. Cytokines |
dc.subject.cnpq.fl_str_mv |
Biociências e Nanomateriais |
description |
Carbon nanotubes (CNT) have unique properties that are of great interest to the biotechnology area. The idea of using CNTs as carriers of drugs, adjuvants for vaccines and biosensors, is attracting great interest from the scientific community. However, the biocompatibility and toxicity in vitro and in vivo of these structures need to be investigated. Thus, the aim of this study was to investigate the cytotoxicity caused by carbon nanotubes multi-walled (MWCNT) in peritoneal macrophages isolated from C57BL/6 mice and the secondary line RAW 264.7. To perform the in vitro test, cultured cells were treated with concentrations of 1 mg/mL and 5mg/mL MWCNT and 1μg/mL of lipopolysaccharide (LPS). The control group of cells with culture medium were cultured in DMEM medium supplemented with 10% fetal bovine serum (FBS) and the negative control at 100 mϻ hydrogen peroxide. The cells remained in treatments for periods of 24 and 48 hours. Then was determined cell viability through the exclusion method using trypan blue, which remained around 70%. After the biochemical cytotoxicity tests were conducted using the release of lactate dehydrogenase (LDH) in the cell supernatant, allowing to note that the concentrations of MWCNT were unable to cause any damage to the cell membrane, as well as any cytotoxic effect. These results confirm the microscopic images, which do not show cell death caused by MWCNT at these concentrations. The citotoxicity caused by MWCNT was evaluated by the measurement of nitric oxide (NO) and the gene expression of TNF-α and IL-1β. In these tests it was revealed that the toxicity was present in cells treated with the secondary line MWCNT, which did not have an increase in NO production and gene expression of cytokines analyzed. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-03-23 |
dc.date.accessioned.fl_str_mv |
2018-08-16T19:45:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Müller, Betina. TOXICIDADE DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM MACRÓFAGOS. 2015. 78f. Dissertação( Programa de Pós-Graduação em Nanociências) - Centro Universitário Franciscano, Santa Maria - RS . |
dc.identifier.uri.fl_str_mv |
http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/536 |
identifier_str_mv |
Müller, Betina. TOXICIDADE DE NANOTUBOS DE CARBONO DE PAREDES MÚLTIPLAS EM MACRÓFAGOS. 2015. 78f. Dissertação( Programa de Pós-Graduação em Nanociências) - Centro Universitário Franciscano, Santa Maria - RS . |
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por |
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Centro Universitário Franciscano |
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Programa de Pós-Graduação em Nanociências |
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UNIFRA |
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Brasil |
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Biociências e Nanomateriais |
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Centro Universitário Franciscano |
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