AVALIAÇÃO DOS EFEITOS DE NANOTUBOS DE CARBONO DE PAREDE MÚLTIPLA CARBOXILADOS EM CÉLULAS DO SISTEMA IMUNOLÓGICO
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Universidade Franciscana |
Texto Completo: | http://tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/254 http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/340 |
Resumo: | The carbon nanotubes (CNTs) display exclusive properties which are of great interest to biotechnology area. The idea of utilizing CTNs as drug carriers, adjuvant for vaccines and biosensors is attracting much interest from scientific community. However, biocompatibility and toxicity in vitro and in vivo of these structures need to be investigated. The aim of this study was to determine the effects of functionalized multiwalled carbon nanotubes (MWCNTs) on cells of the immune system. For in vitro test, splenocytes and bone marrow cells were isolated from BALB/c female mice. The group of treated cells was cultured with carboxylic MWCNTs suspensions at different concentrations (1, 5 or 10 ng/mL), while the control group was only cultured with medium RMPI 1640 and 10% fetal calf serum (FCS). After 24, 48 and 72 hours of cultivation, the viability proliferation and interaction were analyzed by transmission electron microscopy (TEM) analysis of carboxylic MWCNTs with cells. In vivo study, carboxylic MWCNTs were administered intravenously with a concentration of 50 and 100 μg. After 24 hours, splenocytes were isolated, labeled with anti-CD3+ and anti- B220+ antibodies and analyzed by Fluorescence Activated Cell Sorting (FACS). Total IgG production was determined in mice serum after 20 days of administration. The results showed no significant difference (p < 0.05) in the cell viability test of splenocytes, although it has been observed an increase in the frequency of cells directly proportional to the concentrations of carboxylic MWCNTs suspensions in the period of 48 hours. For marrow cells it was observed an inversion in the frequency of cells in the periods of 24 and 48 hours. Regarding the proliferation of splenocytes, groups of cells treated with carboxylic MWCNTs showed significant difference (p<0.05) when compared to control positive group (CpG oligonucleotide). In the analyses performed by TEM, it was not found any type of interaction of carboxylic MWCNTs with splenocytes treated with carboxylic MWCNTs in the concentration of 10 ng/mL. According to the analyses carried out by flow cytometry, the percentage of CD3+ cells and B220+ cells showed no significant difference in relation to the control group. In Enzyme Immunoassay (ELISA), the total IgG antibody production did not increase in the serum of mice treated with carboxylic MWCNTs when compared to control. Thus, the results in vitro and in vivo indicate that carboxylic MWCNTs did not present stimulatory effects on cells of the immune system and, at the same conditions, no cellular toxicity. |
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Rodrigues Junior, Luiz CarlosCPF:63346460010http://lattes.cnpq.br/6354567436180190Fagan, Solange BinottoCPF:63600277049CPF:98952528034http://lattes.cnpq.br/4151122347435671Barbosa, Gabriela de Moraes2018-06-27T18:56:33Z2010-11-09BARBOSA, Gabriela de Moraes. AVALIAÇÃO DOS EFEITOS DE NANOTUBOS DE CARBONO DE PAREDE MÚLTIPLA CARBOXILADOS EM CÉLULAS DO SISTEMA IMUNOLÓGICO. 2010. 52 f. Dissertação (Mestrado em Biociências e Nanomateriais) - Universidade Franciscana, Santa Maria, 2010.http://tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/254http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/340The carbon nanotubes (CNTs) display exclusive properties which are of great interest to biotechnology area. The idea of utilizing CTNs as drug carriers, adjuvant for vaccines and biosensors is attracting much interest from scientific community. However, biocompatibility and toxicity in vitro and in vivo of these structures need to be investigated. The aim of this study was to determine the effects of functionalized multiwalled carbon nanotubes (MWCNTs) on cells of the immune system. For in vitro test, splenocytes and bone marrow cells were isolated from BALB/c female mice. The group of treated cells was cultured with carboxylic MWCNTs suspensions at different concentrations (1, 5 or 10 ng/mL), while the control group was only cultured with medium RMPI 1640 and 10% fetal calf serum (FCS). After 24, 48 and 72 hours of cultivation, the viability proliferation and interaction were analyzed by transmission electron microscopy (TEM) analysis of carboxylic MWCNTs with cells. In vivo study, carboxylic MWCNTs were administered intravenously with a concentration of 50 and 100 μg. After 24 hours, splenocytes were isolated, labeled with anti-CD3+ and anti- B220+ antibodies and analyzed by Fluorescence Activated Cell Sorting (FACS). Total IgG production was determined in mice serum after 20 days of administration. The results showed no significant difference (p < 0.05) in the cell viability test of splenocytes, although it has been observed an increase in the frequency of cells directly proportional to the concentrations of carboxylic MWCNTs suspensions in the period of 48 hours. For marrow cells it was observed an inversion in the frequency of cells in the periods of 24 and 48 hours. Regarding the proliferation of splenocytes, groups of cells treated with carboxylic MWCNTs showed significant difference (p<0.05) when compared to control positive group (CpG oligonucleotide). In the analyses performed by TEM, it was not found any type of interaction of carboxylic MWCNTs with splenocytes treated with carboxylic MWCNTs in the concentration of 10 ng/mL. According to the analyses carried out by flow cytometry, the percentage of CD3+ cells and B220+ cells showed no significant difference in relation to the control group. In Enzyme Immunoassay (ELISA), the total IgG antibody production did not increase in the serum of mice treated with carboxylic MWCNTs when compared to control. Thus, the results in vitro and in vivo indicate that carboxylic MWCNTs did not present stimulatory effects on cells of the immune system and, at the same conditions, no cellular toxicity.Os nanotubos de carbono (NTCs) apresentam propriedades exclusivas que são de grande interesse para a área de biotecnologia. A idéia de se utilizar NTCs como carreadores de fármacos, adjuvantes para vacinas e biosensores, vem despertando muito interesse da comunidade científica. No entanto, a biocompatibilidade e toxicidade in vitro and in vivo dessas estruturas precisam ser investigadas. O objetivo desse estudo foi determinar os efeitos dos nanotubos de carbono de paredes múltiplas (NTCPMs) carboxilados sobre células do sistema imunológico. Para os testes in vitro, esplenócitos e células da medula óssea foram isolados de camundongos BALB/c fêmeas. O grupo de células tratadas foi cultivado com suspensões de NTCPMs carboxilados em diferentes concentrações (1, 5 ou 10 ng/mL), enquanto o grupo controle foi cultivado apenas com meio RMPI 1640 e 10% soro fetal bovino (SFB). Após 24, 48 e 72 horas de cultivo, foram analisadas viabilidade, proliferação e interação por análise de microscopia eletrônica de transmissão (MET) dos NTCPMs carboxilados com as células. No estudo in vivo, NTCPMs carboxilados foram administrados pela via intravenosa com concentração de 50 e 100 μg. Após 24 horas, os esplenócitos foram isolados, marcados com anticorpos anti-CD3+ e anti-B220+ e analisados por citometria de fluxo. A produção de IgG total foi determinada no soro de camundongos após 20 dias da administração. Os resultados encontrados indicaram que não houve diferença significativa (p<0,05) no teste de viabilidade celular de esplenócitos, embora tenha sido observado um aumento na freqüência de células diretamente proporcional às concentrações das suspensões de NTCPMs carboxilados no tempo de 48 horas. Para as células da medula, foi observada uma inversão na frequência de células nos tempos de 24 e 48 horas. Em relação à proliferação de esplenócitos, os grupos de células tratadas com NTCPMs carboxilados apresentaram diferença significativa (p<0,05) quando comparado ao grupo controle positivo (oligonucleotídeo CpG). Nas análises feitas por MET, não foi encontrado qualquer tipo de interação dos NTCPMs carboxilados com esplenócitos tratados com NTCPMs carboxilados na concentração de 10 ng/mL. De acordo com as análises realizadas por citometria de fluxo, a porcentagem de células de CD3+ e células B220+ não apresentaram diferença significativa com relação ao grupo controle. No teste de Enzima Imunoensaio (ELISA), a produção de anticorpos IgG totais não aumentou no soro de camundongos tratados com NTCPMs carboxilados quando comparada a do grupo controle. Sendo assim, os resultados in vitro e in vivo indicam que NTCPMs carboxilados não apresentam efeitos estimulatórios sobre células do sistema imunológico e, nas mesmas condições, não apresentam toxicidade celular.Made available in DSpace on 2018-06-27T18:56:33Z (GMT). No. of bitstreams: 2 Gabriela de Moraes Barbosa.pdf: 1970444 bytes, checksum: cc094467f0ae630c8ca1d2412d92b154 (MD5) Gabriela de Moraes Barbosa.pdf.jpg: 3366 bytes, checksum: 682fcf8d3a97c6bde3382a41872a5ae8 (MD5) Previous issue date: 2010-11-09Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfhttp://tede.universidadefranciscana.edu.br:8080/retrieve/488/Gabriela%20de%20Moraes%20Barbosa.pdf.jpgporUniversidade FranciscanaMestrado Acadêmico em NanociênciasUFNBRBiociências e NanomateriaisNanotubos de carbonoesplenócitosadjuvanteproliferação celularlinfócitoscarbon nanotubessplenocytesadjuvantscell proliferationlymphocytesCNPQ::ENGENHARIASAVALIAÇÃO DOS EFEITOS DE NANOTUBOS DE CARBONO DE PAREDE MÚLTIPLA CARBOXILADOS EM CÉLULAS DO SISTEMA IMUNOLÓGICOinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional Universidade Franciscanainstname:Universidade Franciscana (UFN)instacron:UFNORIGINALGabriela de Moraes Barbosa.pdfapplication/pdf1970444http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/340/1/Gabriela+de+Moraes+Barbosa.pdfcc094467f0ae630c8ca1d2412d92b154MD51THUMBNAILGabriela de Moraes Barbosa.pdf.jpgimage/jpeg3366http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/340/2/Gabriela+de+Moraes+Barbosa.pdf.jpg682fcf8d3a97c6bde3382a41872a5ae8MD52UFN-BDTD/3402018-06-27 15:56:33.452oai:tede.universidadefranciscana.edu.br:UFN-BDTD/340Repositório de Publicaçõeshttp://www.tede.universidadefranciscana.edu.br:8080/http://www.tede.universidadefranciscana.edu.br:8080/oai/requestopendoar:2018-06-27T18:56:33Repositório Institucional Universidade Franciscana - Universidade Franciscana (UFN)false |
dc.title.por.fl_str_mv |
AVALIAÇÃO DOS EFEITOS DE NANOTUBOS DE CARBONO DE PAREDE MÚLTIPLA CARBOXILADOS EM CÉLULAS DO SISTEMA IMUNOLÓGICO |
title |
AVALIAÇÃO DOS EFEITOS DE NANOTUBOS DE CARBONO DE PAREDE MÚLTIPLA CARBOXILADOS EM CÉLULAS DO SISTEMA IMUNOLÓGICO |
spellingShingle |
AVALIAÇÃO DOS EFEITOS DE NANOTUBOS DE CARBONO DE PAREDE MÚLTIPLA CARBOXILADOS EM CÉLULAS DO SISTEMA IMUNOLÓGICO Barbosa, Gabriela de Moraes Nanotubos de carbono esplenócitos adjuvante proliferação celular linfócitos carbon nanotubes splenocytes adjuvants cell proliferation lymphocytes CNPQ::ENGENHARIAS |
title_short |
AVALIAÇÃO DOS EFEITOS DE NANOTUBOS DE CARBONO DE PAREDE MÚLTIPLA CARBOXILADOS EM CÉLULAS DO SISTEMA IMUNOLÓGICO |
title_full |
AVALIAÇÃO DOS EFEITOS DE NANOTUBOS DE CARBONO DE PAREDE MÚLTIPLA CARBOXILADOS EM CÉLULAS DO SISTEMA IMUNOLÓGICO |
title_fullStr |
AVALIAÇÃO DOS EFEITOS DE NANOTUBOS DE CARBONO DE PAREDE MÚLTIPLA CARBOXILADOS EM CÉLULAS DO SISTEMA IMUNOLÓGICO |
title_full_unstemmed |
AVALIAÇÃO DOS EFEITOS DE NANOTUBOS DE CARBONO DE PAREDE MÚLTIPLA CARBOXILADOS EM CÉLULAS DO SISTEMA IMUNOLÓGICO |
title_sort |
AVALIAÇÃO DOS EFEITOS DE NANOTUBOS DE CARBONO DE PAREDE MÚLTIPLA CARBOXILADOS EM CÉLULAS DO SISTEMA IMUNOLÓGICO |
author |
Barbosa, Gabriela de Moraes |
author_facet |
Barbosa, Gabriela de Moraes |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Rodrigues Junior, Luiz Carlos |
dc.contributor.advisor1ID.fl_str_mv |
CPF:63346460010 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6354567436180190 |
dc.contributor.advisor-co1.fl_str_mv |
Fagan, Solange Binotto |
dc.contributor.advisor-co1ID.fl_str_mv |
CPF:63600277049 |
dc.contributor.authorID.fl_str_mv |
CPF:98952528034 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4151122347435671 |
dc.contributor.author.fl_str_mv |
Barbosa, Gabriela de Moraes |
contributor_str_mv |
Rodrigues Junior, Luiz Carlos Fagan, Solange Binotto |
dc.subject.por.fl_str_mv |
Nanotubos de carbono esplenócitos adjuvante proliferação celular linfócitos |
topic |
Nanotubos de carbono esplenócitos adjuvante proliferação celular linfócitos carbon nanotubes splenocytes adjuvants cell proliferation lymphocytes CNPQ::ENGENHARIAS |
dc.subject.eng.fl_str_mv |
carbon nanotubes splenocytes adjuvants cell proliferation lymphocytes |
dc.subject.cnpq.fl_str_mv |
CNPQ::ENGENHARIAS |
description |
The carbon nanotubes (CNTs) display exclusive properties which are of great interest to biotechnology area. The idea of utilizing CTNs as drug carriers, adjuvant for vaccines and biosensors is attracting much interest from scientific community. However, biocompatibility and toxicity in vitro and in vivo of these structures need to be investigated. The aim of this study was to determine the effects of functionalized multiwalled carbon nanotubes (MWCNTs) on cells of the immune system. For in vitro test, splenocytes and bone marrow cells were isolated from BALB/c female mice. The group of treated cells was cultured with carboxylic MWCNTs suspensions at different concentrations (1, 5 or 10 ng/mL), while the control group was only cultured with medium RMPI 1640 and 10% fetal calf serum (FCS). After 24, 48 and 72 hours of cultivation, the viability proliferation and interaction were analyzed by transmission electron microscopy (TEM) analysis of carboxylic MWCNTs with cells. In vivo study, carboxylic MWCNTs were administered intravenously with a concentration of 50 and 100 μg. After 24 hours, splenocytes were isolated, labeled with anti-CD3+ and anti- B220+ antibodies and analyzed by Fluorescence Activated Cell Sorting (FACS). Total IgG production was determined in mice serum after 20 days of administration. The results showed no significant difference (p < 0.05) in the cell viability test of splenocytes, although it has been observed an increase in the frequency of cells directly proportional to the concentrations of carboxylic MWCNTs suspensions in the period of 48 hours. For marrow cells it was observed an inversion in the frequency of cells in the periods of 24 and 48 hours. Regarding the proliferation of splenocytes, groups of cells treated with carboxylic MWCNTs showed significant difference (p<0.05) when compared to control positive group (CpG oligonucleotide). In the analyses performed by TEM, it was not found any type of interaction of carboxylic MWCNTs with splenocytes treated with carboxylic MWCNTs in the concentration of 10 ng/mL. According to the analyses carried out by flow cytometry, the percentage of CD3+ cells and B220+ cells showed no significant difference in relation to the control group. In Enzyme Immunoassay (ELISA), the total IgG antibody production did not increase in the serum of mice treated with carboxylic MWCNTs when compared to control. Thus, the results in vitro and in vivo indicate that carboxylic MWCNTs did not present stimulatory effects on cells of the immune system and, at the same conditions, no cellular toxicity. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-11-09 |
dc.date.accessioned.fl_str_mv |
2018-06-27T18:56:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BARBOSA, Gabriela de Moraes. AVALIAÇÃO DOS EFEITOS DE NANOTUBOS DE CARBONO DE PAREDE MÚLTIPLA CARBOXILADOS EM CÉLULAS DO SISTEMA IMUNOLÓGICO. 2010. 52 f. Dissertação (Mestrado em Biociências e Nanomateriais) - Universidade Franciscana, Santa Maria, 2010. |
dc.identifier.uri.fl_str_mv |
http://tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/254 http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/340 |
identifier_str_mv |
BARBOSA, Gabriela de Moraes. AVALIAÇÃO DOS EFEITOS DE NANOTUBOS DE CARBONO DE PAREDE MÚLTIPLA CARBOXILADOS EM CÉLULAS DO SISTEMA IMUNOLÓGICO. 2010. 52 f. Dissertação (Mestrado em Biociências e Nanomateriais) - Universidade Franciscana, Santa Maria, 2010. |
url |
http://tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/254 http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/340 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Franciscana |
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Mestrado Acadêmico em Nanociências |
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UFN |
dc.publisher.country.fl_str_mv |
BR |
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Biociências e Nanomateriais |
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Universidade Franciscana |
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