ATIVIDADE DA NTPDase DE LINFÓCITOS NA DERMATITE DE CONTATO ANTES E APÓS TRATAMENTO COM DEXAMETASONA NANOESTRUTURADA
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Universidade Franciscana |
Texto Completo: | http://tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/235 http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/318 |
Resumo: | Since the extracellular nucleotides represent an important means of modulating the activity of lymphocytes, it is essential the presence of an enzymatic mechanism to keep constant the concentration of those in the extracellular space. The activity of NTPDase has been recognized as a marker of activation necessary for the function of effector lymphocytes, participate in the processes of recognition of antigen. Contact dermatitis occurs in a delayed hypersensitivity reaction of type IV, mediated by cells through a mechanism that sensitizes the immune T lymphocyte to an antigen protein or a hapten linked to a protein. Among the mediators able to modulate the actions of lymphocytes stand out from the nucleoside and nucleotide adenine, in particular the extracellular ATP that is able to regulate the cell-cell interactions are important processes of activation, differentiation, development, proliferation, cell death and responses of effector lymphocytes. This study sought to determine first of the hydrolysis of adenine nucleotides, the NTPDase (EC 3.6.1.5, nucleoside triphosphate difosfoidrolase, CD39) in lymphocytes from mice with dermatitis induced by nickel sulphate to 5%, before and after treatment with dexamethasone and dexamethasone nanostructured free to try to check the possible changes in the activity of this enzyme front of an inflammatory reaction of type IV hypersensitivity and immunosuppressive therapy. Moreover, it was possible to verify the relationship of submission of the drug in the formulation free and nanostructures with the hydrolysis of adenine nucleotides. The average enzymatic activity of the group with contact dermatitis was significantly higher in the control group by the test of hypotheses to averages T. The results are in line with work done earlier that showed an increase of enzyme activity by the activation of lymphocytes. The hydrolysis of ATP and ADP in the group treated with dexamethasone free and in the group treated with dexamethasone nanostructured was significantly higher in the control group by analysis of variance for a way (ANOVA) followed by Kruskal-Wallis test (P < 0001). The results are in line with work done earlier that showed an increase of enzyme activity by the activation of lymphocytes. Was observed greater hydrolysis of ATP and ADP in the group treated with dexamethasone nanostructures in relation to the group treated with dexamethasone free. However, this difference was not statistically significant. Work previously shown an increase of enzyme activity during treatment with dexamethasone as a possible compensatory effect of the decrease in the number of lymphocytes. The results of this study suggest that dexamethasone nanostructures possess a immunosuppressive effects greatest, which may be the beginning of the evaluation of a more effective and safe treatment for contact dermatitis. From these results we can conclude that the determination of the activity of NTPDase in lymphocytes could be used as an indicator of the efficiency of the treatment of contact dermatitis |
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Leal, Daniela Bitencourt RosaCPF:55822983049CPF:88440230087Brum, Liliani Mathias2018-06-27T18:56:21Z2008-09-18BRUM, Liliani Mathias. ATIVIDADE DA NTPDase DE LINFÓCITOS NA DERMATITE DE CONTATO ANTES E APÓS TRATAMENTO COM DEXAMETASONA NANOESTRUTURADA. 2008. 75 f. Dissertação (Mestrado em Biociências e Nanomateriais) - Universidade Franciscana, Santa Maria, 2008.http://tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/235http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/318Since the extracellular nucleotides represent an important means of modulating the activity of lymphocytes, it is essential the presence of an enzymatic mechanism to keep constant the concentration of those in the extracellular space. The activity of NTPDase has been recognized as a marker of activation necessary for the function of effector lymphocytes, participate in the processes of recognition of antigen. Contact dermatitis occurs in a delayed hypersensitivity reaction of type IV, mediated by cells through a mechanism that sensitizes the immune T lymphocyte to an antigen protein or a hapten linked to a protein. Among the mediators able to modulate the actions of lymphocytes stand out from the nucleoside and nucleotide adenine, in particular the extracellular ATP that is able to regulate the cell-cell interactions are important processes of activation, differentiation, development, proliferation, cell death and responses of effector lymphocytes. This study sought to determine first of the hydrolysis of adenine nucleotides, the NTPDase (EC 3.6.1.5, nucleoside triphosphate difosfoidrolase, CD39) in lymphocytes from mice with dermatitis induced by nickel sulphate to 5%, before and after treatment with dexamethasone and dexamethasone nanostructured free to try to check the possible changes in the activity of this enzyme front of an inflammatory reaction of type IV hypersensitivity and immunosuppressive therapy. Moreover, it was possible to verify the relationship of submission of the drug in the formulation free and nanostructures with the hydrolysis of adenine nucleotides. The average enzymatic activity of the group with contact dermatitis was significantly higher in the control group by the test of hypotheses to averages T. The results are in line with work done earlier that showed an increase of enzyme activity by the activation of lymphocytes. The hydrolysis of ATP and ADP in the group treated with dexamethasone free and in the group treated with dexamethasone nanostructured was significantly higher in the control group by analysis of variance for a way (ANOVA) followed by Kruskal-Wallis test (P < 0001). The results are in line with work done earlier that showed an increase of enzyme activity by the activation of lymphocytes. Was observed greater hydrolysis of ATP and ADP in the group treated with dexamethasone nanostructures in relation to the group treated with dexamethasone free. However, this difference was not statistically significant. Work previously shown an increase of enzyme activity during treatment with dexamethasone as a possible compensatory effect of the decrease in the number of lymphocytes. The results of this study suggest that dexamethasone nanostructures possess a immunosuppressive effects greatest, which may be the beginning of the evaluation of a more effective and safe treatment for contact dermatitis. From these results we can conclude that the determination of the activity of NTPDase in lymphocytes could be used as an indicator of the efficiency of the treatment of contact dermatitisUma vez que os nucleotídeos extracelulares representam uma importante via de modulação da atividade dos linfócitos, é indispensável a presença de um mecanismo enzimático capaz de manter constante a concentração desses no espaço extracelular. A atividade da NTPDase tem sido reconhecida como um marcador de ativação necessário para a função efetora dos linfócitos, participando também dos processos de reconhecimento do antígeno. Na dermatite de contato ocorre uma reação de hipersensibilidade retardada tipo IV, mediada por células, através de um mecanismo imunológico que sensibiliza os linfócitos T frente a um antígeno protéico ou a um hapteno ligado a uma proteína. Dentre os mediadores capazes de modular as ações dos linfócitos destacam-se os nucleosídeos e nucleotídeos da adenina, em especial o ATP extracelular que é capaz de regular as interações célula-célula sendo importante nos processos de ativação, diferenciação, desenvolvimento, proliferação, morte celular e respostas efetoras dos linfócitos. Este estudo procurou determinar primeiramente a hidrólise de nucleotídeos da adenina, pela NTPDase (EC 3.6.1.5, nucleosídeo trifosfato difosfoidrolase, CD39) em linfócitos de ratos com dermatite induzida por sulfato de níquel, antes e após tratamento com dexametasona livre e dexametasona nanoestruturada, para tentar verificar as possíveis alterações na atividade desta enzima frente a uma reação inflamatória de hipersensibilidade tipo IV e na terapia imunossupressora. Além disso, procurou-se verificar a possível relação da apresentação do fármaco na formulação livre e nanoestruturada com a hidrólise de nucleotídeos da adenina. A atividade enzimática média do grupo com dermatite de contato foi significativamente maior em relação ao grupo controle pelo teste de hipóteses para médias T. Os resultados encontrados estão de acordo com trabalhos realizados anteriormente que demonstram um aumento da atividade enzimática pela ativação dos linfócitos. A hidrólise do ATP e do ADP no grupo tratado com dexametasona livre e no grupo tratado com dexametasona nanoestruturada foi significativamente maior em relação ao grupo controle pelo teste de análise de variância de uma via (ANOVA) seguido pelo teste de Kruskal-Wallis (P< 0,001). Observou-se uma maior hidrólise de ADP e ATP, no grupo tratado com dexametasona nanoestruturada em relação ao grupo tratado com dexametasona livre. No entanto, esta diferença não foi estatisticamente significativa. Trabalhos anteriores já demonstraram um aumento de atividade enzimática durante tratamento com dexametasona como um possível efeito compensatório à diminuição do número de linfócitos. Os resultados deste estudo sugerem que a dexametasona nanoestruturada possui um efeito imunossupressor maior, o que pode ser o início da avaliação de um tratamento mais eficaz e seguro para a dermatite de contato. A partir destes resultados podemos concluir que a determinação da atividade da NTPDase em linfócitos poderia ser utilizada como um indicador da eficiência da terapêutica da dermatite de contato.Made available in DSpace on 2018-06-27T18:56:21Z (GMT). No. of bitstreams: 2 Liliani Mathias Brum.pdf: 1503888 bytes, checksum: ad2eacb7dc98b3e5fbf2604c9ae82084 (MD5) Liliani Mathias Brum.pdf.jpg: 3065 bytes, checksum: 9adf1c37fd40ed3180ec5d7ac9fc8754 (MD5) Previous issue date: 2008-09-18Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfhttp://tede.universidadefranciscana.edu.br:8080/retrieve/470/Liliani%20Mathias%20Brum.pdf.jpgporUniversidade FranciscanaMestrado Acadêmico em NanociênciasUFNBRBiociências e NanomateriaisNTPDaseDermatite de ContatoDexametasonaCD39NTPDaseContact DermatitisDexamethasoneCD39CNPQ::ENGENHARIASATIVIDADE DA NTPDase DE LINFÓCITOS NA DERMATITE DE CONTATO ANTES E APÓS TRATAMENTO COM DEXAMETASONA NANOESTRUTURADAinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional Universidade Franciscanainstname:Universidade Franciscana (UFN)instacron:UFNORIGINALLiliani Mathias Brum.pdfapplication/pdf1503888http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/318/1/Liliani+Mathias+Brum.pdfad2eacb7dc98b3e5fbf2604c9ae82084MD51THUMBNAILLiliani Mathias Brum.pdf.jpgimage/jpeg3065http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/318/2/Liliani+Mathias+Brum.pdf.jpg9adf1c37fd40ed3180ec5d7ac9fc8754MD52UFN-BDTD/3182018-06-27 15:56:21.555oai:tede.universidadefranciscana.edu.br:UFN-BDTD/318Repositório de Publicaçõeshttp://www.tede.universidadefranciscana.edu.br:8080/http://www.tede.universidadefranciscana.edu.br:8080/oai/requestopendoar:2018-06-27T18:56:21Repositório Institucional Universidade Franciscana - Universidade Franciscana (UFN)false |
dc.title.por.fl_str_mv |
ATIVIDADE DA NTPDase DE LINFÓCITOS NA DERMATITE DE CONTATO ANTES E APÓS TRATAMENTO COM DEXAMETASONA NANOESTRUTURADA |
title |
ATIVIDADE DA NTPDase DE LINFÓCITOS NA DERMATITE DE CONTATO ANTES E APÓS TRATAMENTO COM DEXAMETASONA NANOESTRUTURADA |
spellingShingle |
ATIVIDADE DA NTPDase DE LINFÓCITOS NA DERMATITE DE CONTATO ANTES E APÓS TRATAMENTO COM DEXAMETASONA NANOESTRUTURADA Brum, Liliani Mathias NTPDase Dermatite de Contato Dexametasona CD39 NTPDase Contact Dermatitis Dexamethasone CD39 CNPQ::ENGENHARIAS |
title_short |
ATIVIDADE DA NTPDase DE LINFÓCITOS NA DERMATITE DE CONTATO ANTES E APÓS TRATAMENTO COM DEXAMETASONA NANOESTRUTURADA |
title_full |
ATIVIDADE DA NTPDase DE LINFÓCITOS NA DERMATITE DE CONTATO ANTES E APÓS TRATAMENTO COM DEXAMETASONA NANOESTRUTURADA |
title_fullStr |
ATIVIDADE DA NTPDase DE LINFÓCITOS NA DERMATITE DE CONTATO ANTES E APÓS TRATAMENTO COM DEXAMETASONA NANOESTRUTURADA |
title_full_unstemmed |
ATIVIDADE DA NTPDase DE LINFÓCITOS NA DERMATITE DE CONTATO ANTES E APÓS TRATAMENTO COM DEXAMETASONA NANOESTRUTURADA |
title_sort |
ATIVIDADE DA NTPDase DE LINFÓCITOS NA DERMATITE DE CONTATO ANTES E APÓS TRATAMENTO COM DEXAMETASONA NANOESTRUTURADA |
author |
Brum, Liliani Mathias |
author_facet |
Brum, Liliani Mathias |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Leal, Daniela Bitencourt Rosa |
dc.contributor.advisor1ID.fl_str_mv |
CPF:55822983049 |
dc.contributor.authorID.fl_str_mv |
CPF:88440230087 |
dc.contributor.author.fl_str_mv |
Brum, Liliani Mathias |
contributor_str_mv |
Leal, Daniela Bitencourt Rosa |
dc.subject.por.fl_str_mv |
NTPDase Dermatite de Contato Dexametasona CD39 |
topic |
NTPDase Dermatite de Contato Dexametasona CD39 NTPDase Contact Dermatitis Dexamethasone CD39 CNPQ::ENGENHARIAS |
dc.subject.eng.fl_str_mv |
NTPDase Contact Dermatitis Dexamethasone CD39 |
dc.subject.cnpq.fl_str_mv |
CNPQ::ENGENHARIAS |
description |
Since the extracellular nucleotides represent an important means of modulating the activity of lymphocytes, it is essential the presence of an enzymatic mechanism to keep constant the concentration of those in the extracellular space. The activity of NTPDase has been recognized as a marker of activation necessary for the function of effector lymphocytes, participate in the processes of recognition of antigen. Contact dermatitis occurs in a delayed hypersensitivity reaction of type IV, mediated by cells through a mechanism that sensitizes the immune T lymphocyte to an antigen protein or a hapten linked to a protein. Among the mediators able to modulate the actions of lymphocytes stand out from the nucleoside and nucleotide adenine, in particular the extracellular ATP that is able to regulate the cell-cell interactions are important processes of activation, differentiation, development, proliferation, cell death and responses of effector lymphocytes. This study sought to determine first of the hydrolysis of adenine nucleotides, the NTPDase (EC 3.6.1.5, nucleoside triphosphate difosfoidrolase, CD39) in lymphocytes from mice with dermatitis induced by nickel sulphate to 5%, before and after treatment with dexamethasone and dexamethasone nanostructured free to try to check the possible changes in the activity of this enzyme front of an inflammatory reaction of type IV hypersensitivity and immunosuppressive therapy. Moreover, it was possible to verify the relationship of submission of the drug in the formulation free and nanostructures with the hydrolysis of adenine nucleotides. The average enzymatic activity of the group with contact dermatitis was significantly higher in the control group by the test of hypotheses to averages T. The results are in line with work done earlier that showed an increase of enzyme activity by the activation of lymphocytes. The hydrolysis of ATP and ADP in the group treated with dexamethasone free and in the group treated with dexamethasone nanostructured was significantly higher in the control group by analysis of variance for a way (ANOVA) followed by Kruskal-Wallis test (P < 0001). The results are in line with work done earlier that showed an increase of enzyme activity by the activation of lymphocytes. Was observed greater hydrolysis of ATP and ADP in the group treated with dexamethasone nanostructures in relation to the group treated with dexamethasone free. However, this difference was not statistically significant. Work previously shown an increase of enzyme activity during treatment with dexamethasone as a possible compensatory effect of the decrease in the number of lymphocytes. The results of this study suggest that dexamethasone nanostructures possess a immunosuppressive effects greatest, which may be the beginning of the evaluation of a more effective and safe treatment for contact dermatitis. From these results we can conclude that the determination of the activity of NTPDase in lymphocytes could be used as an indicator of the efficiency of the treatment of contact dermatitis |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-09-18 |
dc.date.accessioned.fl_str_mv |
2018-06-27T18:56:21Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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BRUM, Liliani Mathias. ATIVIDADE DA NTPDase DE LINFÓCITOS NA DERMATITE DE CONTATO ANTES E APÓS TRATAMENTO COM DEXAMETASONA NANOESTRUTURADA. 2008. 75 f. Dissertação (Mestrado em Biociências e Nanomateriais) - Universidade Franciscana, Santa Maria, 2008. |
dc.identifier.uri.fl_str_mv |
http://tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/235 http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/318 |
identifier_str_mv |
BRUM, Liliani Mathias. ATIVIDADE DA NTPDase DE LINFÓCITOS NA DERMATITE DE CONTATO ANTES E APÓS TRATAMENTO COM DEXAMETASONA NANOESTRUTURADA. 2008. 75 f. Dissertação (Mestrado em Biociências e Nanomateriais) - Universidade Franciscana, Santa Maria, 2008. |
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http://tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/235 http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/318 |
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Universidade Franciscana |
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Mestrado Acadêmico em Nanociências |
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UFN |
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BR |
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Biociências e Nanomateriais |
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Universidade Franciscana |
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