EFEITO DAS β-CICLODEXTRINAS SOBRE PARÂMETROS BIOQUÍMICOS, DO METABOLISMO ENERGÉTICO E DO ESTRESSE OXIDATIVO EM RATOS WISTAR
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Universidade Franciscana |
Texto Completo: | http://tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/197 http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/289 |
Resumo: | Cyclodextrins (CDs) are cyclic oligosaccharides formed by 6 (αCD), 7 (bCD) or 8 (γCD) glucose units with an internal hydrophobic cavity and outside surface hydrophilic. These three derivatives, the b-cyclodextrin (bCD) seems to be the most advantageous for pharmaceutical use for their availability, cavity size and low cost. The CDs have a future quite promising for their properties as greater absorption of drugs through the biological barriers and time of release, however, some types may not be considered non-toxic. The objective of this study was to investigate the intraperitoneal administration of βCD, M-β-CD and HP-ß-CD for 8 weeks with administered dose of 65.65 mg of CDs/kg rats/day, on parameters of biochemical analyzes, enzymes of energy metabolism, enzymes tiolicas sensitive to increase reactive oxygen species and to make this relationship, also evaluate parameters of oxidative stress in cerebral cortex, liver, kidneys and heart of wistar rats. The results showed that for the group treated with βCD there has been a significant increase in serum urea and creatinine levels, indicating nephrotoxicity, however not related to the other parameters. There was also a great reduction in serum levels of iron for the 3 CDs. The heart showed a reduction in the activity of CKmitocondrial and increase for AK by M-β-CD and reduction of CKmit by HP-ß-CD, but showed a reduction in the levels of diclorofluorceina (DCF) to the 3 CDs and protein carbonyl) by βCD. For the rim there was no significant change in comreducao activity of CKmit by HP-β-CD. In liver tissue, the βCD and M-β-CD reduced the activity of PK, but this is not reflected in blood glucose levels. In the cerebral cortex, the βCD reduced the activity of enzymes CK mitochondrial and PK, also reduced TBARS, but increased carbonyl protein. The indices lipidemic reduced reported by other researchers was not observed in this work, because the group of M-β- CD has a significant increase in serum levels of LDL cholesterol, in addition to aspartate aminostransferase AST, albumin, total protein, alkaline phosphatase, sodium, calcium, magnesium and phosphate. Our results indicate that some CDs alter enzymes crucial for energy metabolism, mainly of brain tissue with a reduction in activity and the PK by βCD. If changes in the activity of these enzymes occur in people who use drugs by intraperitoneal route, it is possible that the energy metabolism and brain functioning may be affected causing damage to the tissue. However more studies are needed to elucidate how there was a reduction of serum iron and as the cyclodextrins affect a structure so well protected by blood-brain barrier as the brain. |
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Rech, Virginia CieloCPF:58768211015Silva, Ivana Zanella daCPF:94305382091http://lattes.cnpq.br/7107334055877180CPF:00077644050Oliveira, Amanda Lima de2018-06-27T18:56:01Z2012-11-30OLIVEIRA, Amanda Lima de. EFEITO DAS β-CICLODEXTRINAS SOBRE PARÂMETROS BIOQUÍMICOS, DO METABOLISMO ENERGÉTICO E DO ESTRESSE OXIDATIVO EM RATOS WISTAR. 2012. 55 f. Dissertação (Mestrado em Biociências e Nanomateriais) - Universidade Franciscana, Santa Maria, 2012.http://tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/197http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/289Cyclodextrins (CDs) are cyclic oligosaccharides formed by 6 (αCD), 7 (bCD) or 8 (γCD) glucose units with an internal hydrophobic cavity and outside surface hydrophilic. These three derivatives, the b-cyclodextrin (bCD) seems to be the most advantageous for pharmaceutical use for their availability, cavity size and low cost. The CDs have a future quite promising for their properties as greater absorption of drugs through the biological barriers and time of release, however, some types may not be considered non-toxic. The objective of this study was to investigate the intraperitoneal administration of βCD, M-β-CD and HP-ß-CD for 8 weeks with administered dose of 65.65 mg of CDs/kg rats/day, on parameters of biochemical analyzes, enzymes of energy metabolism, enzymes tiolicas sensitive to increase reactive oxygen species and to make this relationship, also evaluate parameters of oxidative stress in cerebral cortex, liver, kidneys and heart of wistar rats. The results showed that for the group treated with βCD there has been a significant increase in serum urea and creatinine levels, indicating nephrotoxicity, however not related to the other parameters. There was also a great reduction in serum levels of iron for the 3 CDs. The heart showed a reduction in the activity of CKmitocondrial and increase for AK by M-β-CD and reduction of CKmit by HP-ß-CD, but showed a reduction in the levels of diclorofluorceina (DCF) to the 3 CDs and protein carbonyl) by βCD. For the rim there was no significant change in comreducao activity of CKmit by HP-β-CD. In liver tissue, the βCD and M-β-CD reduced the activity of PK, but this is not reflected in blood glucose levels. In the cerebral cortex, the βCD reduced the activity of enzymes CK mitochondrial and PK, also reduced TBARS, but increased carbonyl protein. The indices lipidemic reduced reported by other researchers was not observed in this work, because the group of M-β- CD has a significant increase in serum levels of LDL cholesterol, in addition to aspartate aminostransferase AST, albumin, total protein, alkaline phosphatase, sodium, calcium, magnesium and phosphate. Our results indicate that some CDs alter enzymes crucial for energy metabolism, mainly of brain tissue with a reduction in activity and the PK by βCD. If changes in the activity of these enzymes occur in people who use drugs by intraperitoneal route, it is possible that the energy metabolism and brain functioning may be affected causing damage to the tissue. However more studies are needed to elucidate how there was a reduction of serum iron and as the cyclodextrins affect a structure so well protected by blood-brain barrier as the brain.As ciclodextrinas (CDs) são oligossacarídeos cíclicos formados por 6 (αCD), 7 (bCD) ou 8 (γCD) unidades de glicose com uma cavidade interna hidrofóbica e superfície externa hidrofílica. Destes três derivados, a b-ciclodextrina (bCD) parece ser a mais vantajosa para utilização farmacêutica pela sua disponibilidade, tamanho da cavidade e baixo custo. O interesse pelas CDs se dá pelas suas propriedades como maior absorção dos fármacos através das barreiras biológicas e tempo de liberação, entretanto, alguns tipos não podem ser considerados atóxicas. O objetivo deste estudo foi investigar a administração intraperitoneal de βCD (Beta Ciclodextrina), M-β-CD (Metil Beta Ciclodextrina) e HP-β-CD (Hidroxypropil Beta Ciclodextrina) durante 8 semanas com dose administrada de 65,65 mg das CDs/kg rato/dia, sobre parâmetros de análises bioquímicas, de enzimas do metabolismo energético, enzimas tiólicas sensíveis ao aumento de espécies reativas e para fazer a relação, também avaliar parâmetros de estresse oxidativo em córtex cerebral, fígado, rins e coração de ratos wistar. Os resultados mostraram que para o grupo tratado com βCD houve um aumento significativo nos níveis séricos de uréia e creatinina, indicando nefrotoxidade, porém não relacionada com os demais parâmetros. Também houve uma grande redução nos níveis séricos de ferro para as 3 CDs. O coração apresentou redução na atividade da Creatinaquinase mitocondrial (CKmit) e aumento para Adenilatoquinase (AK) pela M- β-CD e redução da CKmit pela HP-β-CD, porém apresentou uma redução nos níveis de diclorofluoresceína (DCF) para as 3 CDs e carbonilas proteicas pela βCD. Para o rim houve alteração significativa com redução na atividade da CKmit pela HP-β-CD. No tecido hepático, a βCD e M-β-CD reduziram a atividade da Piruvatoquinase (PK), porém isto não refletiu nos níveis glicêmicos. No córtex cerebral, a βCD reduziu a atividade das enzimas CK mitocondrial e PK, também reduziu TBARS (Espécies reativas ao ácido tiobarbitúrico), mas aumentou carbonilas proteicas. Os índices lipidêmicos reduzidos relatados por outros pesquisadores não foi observado neste trabalho, pois o grupo da M-β-CD apresentou um aumento significativo nos níveis séricos de LDL (lipoproteína de baixa densidade), além de AST (aspartato aminostransferase), albumina, proteínas totais, fosfatase alcalina, sódio, cálcio, magnésio e fosfato. Os resultados indicam que algumas CDs alteram enzimas cruciais do metabolismo energético, principalmente do tecido cerebral com redução na atividade da PK pela βCD. Possíveis alterações na atividade destas enzimas podem afetar o metabolismo energético e o funcionamento cerebral causando dano ao tecido. Entretanto mais estudos são necessários para elucidar de que forma ocorreu a redução sérica de ferro e como as ciclodextrinas afetaram uma estrutura tão bem protegida pela barreira hemato-encefálica como a cerebral.Made available in DSpace on 2018-06-27T18:56:01Z (GMT). No. of bitstreams: 2 Amanda Lima de Oliveira.pdf: 513074 bytes, checksum: 393de3a3c8893cb2dad143281cc76ca4 (MD5) Amanda Lima de Oliveira.pdf.jpg: 3435 bytes, checksum: d98e4a9d95435991a70b3e949f124696 (MD5) Previous issue date: 2012-11-30Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfhttp://tede.universidadefranciscana.edu.br:8080/retrieve/446/Amanda%20Lima%20de%20Oliveira.pdf.jpgporUniversidade FranciscanaMestrado Acadêmico em NanociênciasUFNBRBiociências e NanomateriaisNanocarreadorPiruvatoquinaseAdenilatoquinaseCreatinaquinaseToxicidadeNanocarrierPyruvate kinaseAdenylate kinaseCreatine KinaseToxicityCNPQ::ENGENHARIASEFEITO DAS β-CICLODEXTRINAS SOBRE PARÂMETROS BIOQUÍMICOS, DO METABOLISMO ENERGÉTICO E DO ESTRESSE OXIDATIVO EM RATOS WISTARinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional Universidade Franciscanainstname:Universidade Franciscana (UFN)instacron:UFNORIGINALAmanda Lima de Oliveira.pdfapplication/pdf513074http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/289/1/Amanda+Lima+de+Oliveira.pdf393de3a3c8893cb2dad143281cc76ca4MD51THUMBNAILAmanda Lima de Oliveira.pdf.jpgimage/jpeg3435http://tede.universidadefranciscana.edu.br:8080/bitstream/UFN-BDTD/289/2/Amanda+Lima+de+Oliveira.pdf.jpgd98e4a9d95435991a70b3e949f124696MD52UFN-BDTD/2892018-06-27 15:56:01.87oai:tede.universidadefranciscana.edu.br:UFN-BDTD/289Repositório de Publicaçõeshttp://www.tede.universidadefranciscana.edu.br:8080/http://www.tede.universidadefranciscana.edu.br:8080/oai/requestopendoar:2018-06-27T18:56:01Repositório Institucional Universidade Franciscana - Universidade Franciscana (UFN)false |
dc.title.por.fl_str_mv |
EFEITO DAS β-CICLODEXTRINAS SOBRE PARÂMETROS BIOQUÍMICOS, DO METABOLISMO ENERGÉTICO E DO ESTRESSE OXIDATIVO EM RATOS WISTAR |
title |
EFEITO DAS β-CICLODEXTRINAS SOBRE PARÂMETROS BIOQUÍMICOS, DO METABOLISMO ENERGÉTICO E DO ESTRESSE OXIDATIVO EM RATOS WISTAR |
spellingShingle |
EFEITO DAS β-CICLODEXTRINAS SOBRE PARÂMETROS BIOQUÍMICOS, DO METABOLISMO ENERGÉTICO E DO ESTRESSE OXIDATIVO EM RATOS WISTAR Oliveira, Amanda Lima de Nanocarreador Piruvatoquinase Adenilatoquinase Creatinaquinase Toxicidade Nanocarrier Pyruvate kinase Adenylate kinase Creatine Kinase Toxicity CNPQ::ENGENHARIAS |
title_short |
EFEITO DAS β-CICLODEXTRINAS SOBRE PARÂMETROS BIOQUÍMICOS, DO METABOLISMO ENERGÉTICO E DO ESTRESSE OXIDATIVO EM RATOS WISTAR |
title_full |
EFEITO DAS β-CICLODEXTRINAS SOBRE PARÂMETROS BIOQUÍMICOS, DO METABOLISMO ENERGÉTICO E DO ESTRESSE OXIDATIVO EM RATOS WISTAR |
title_fullStr |
EFEITO DAS β-CICLODEXTRINAS SOBRE PARÂMETROS BIOQUÍMICOS, DO METABOLISMO ENERGÉTICO E DO ESTRESSE OXIDATIVO EM RATOS WISTAR |
title_full_unstemmed |
EFEITO DAS β-CICLODEXTRINAS SOBRE PARÂMETROS BIOQUÍMICOS, DO METABOLISMO ENERGÉTICO E DO ESTRESSE OXIDATIVO EM RATOS WISTAR |
title_sort |
EFEITO DAS β-CICLODEXTRINAS SOBRE PARÂMETROS BIOQUÍMICOS, DO METABOLISMO ENERGÉTICO E DO ESTRESSE OXIDATIVO EM RATOS WISTAR |
author |
Oliveira, Amanda Lima de |
author_facet |
Oliveira, Amanda Lima de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Rech, Virginia Cielo |
dc.contributor.advisor1ID.fl_str_mv |
CPF:58768211015 |
dc.contributor.advisor-co1.fl_str_mv |
Silva, Ivana Zanella da |
dc.contributor.advisor-co1ID.fl_str_mv |
CPF:94305382091 |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/7107334055877180 |
dc.contributor.authorID.fl_str_mv |
CPF:00077644050 |
dc.contributor.author.fl_str_mv |
Oliveira, Amanda Lima de |
contributor_str_mv |
Rech, Virginia Cielo Silva, Ivana Zanella da |
dc.subject.por.fl_str_mv |
Nanocarreador Piruvatoquinase Adenilatoquinase Creatinaquinase Toxicidade |
topic |
Nanocarreador Piruvatoquinase Adenilatoquinase Creatinaquinase Toxicidade Nanocarrier Pyruvate kinase Adenylate kinase Creatine Kinase Toxicity CNPQ::ENGENHARIAS |
dc.subject.eng.fl_str_mv |
Nanocarrier Pyruvate kinase Adenylate kinase Creatine Kinase Toxicity |
dc.subject.cnpq.fl_str_mv |
CNPQ::ENGENHARIAS |
description |
Cyclodextrins (CDs) are cyclic oligosaccharides formed by 6 (αCD), 7 (bCD) or 8 (γCD) glucose units with an internal hydrophobic cavity and outside surface hydrophilic. These three derivatives, the b-cyclodextrin (bCD) seems to be the most advantageous for pharmaceutical use for their availability, cavity size and low cost. The CDs have a future quite promising for their properties as greater absorption of drugs through the biological barriers and time of release, however, some types may not be considered non-toxic. The objective of this study was to investigate the intraperitoneal administration of βCD, M-β-CD and HP-ß-CD for 8 weeks with administered dose of 65.65 mg of CDs/kg rats/day, on parameters of biochemical analyzes, enzymes of energy metabolism, enzymes tiolicas sensitive to increase reactive oxygen species and to make this relationship, also evaluate parameters of oxidative stress in cerebral cortex, liver, kidneys and heart of wistar rats. The results showed that for the group treated with βCD there has been a significant increase in serum urea and creatinine levels, indicating nephrotoxicity, however not related to the other parameters. There was also a great reduction in serum levels of iron for the 3 CDs. The heart showed a reduction in the activity of CKmitocondrial and increase for AK by M-β-CD and reduction of CKmit by HP-ß-CD, but showed a reduction in the levels of diclorofluorceina (DCF) to the 3 CDs and protein carbonyl) by βCD. For the rim there was no significant change in comreducao activity of CKmit by HP-β-CD. In liver tissue, the βCD and M-β-CD reduced the activity of PK, but this is not reflected in blood glucose levels. In the cerebral cortex, the βCD reduced the activity of enzymes CK mitochondrial and PK, also reduced TBARS, but increased carbonyl protein. The indices lipidemic reduced reported by other researchers was not observed in this work, because the group of M-β- CD has a significant increase in serum levels of LDL cholesterol, in addition to aspartate aminostransferase AST, albumin, total protein, alkaline phosphatase, sodium, calcium, magnesium and phosphate. Our results indicate that some CDs alter enzymes crucial for energy metabolism, mainly of brain tissue with a reduction in activity and the PK by βCD. If changes in the activity of these enzymes occur in people who use drugs by intraperitoneal route, it is possible that the energy metabolism and brain functioning may be affected causing damage to the tissue. However more studies are needed to elucidate how there was a reduction of serum iron and as the cyclodextrins affect a structure so well protected by blood-brain barrier as the brain. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-11-30 |
dc.date.accessioned.fl_str_mv |
2018-06-27T18:56:01Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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dc.identifier.citation.fl_str_mv |
OLIVEIRA, Amanda Lima de. EFEITO DAS β-CICLODEXTRINAS SOBRE PARÂMETROS BIOQUÍMICOS, DO METABOLISMO ENERGÉTICO E DO ESTRESSE OXIDATIVO EM RATOS WISTAR. 2012. 55 f. Dissertação (Mestrado em Biociências e Nanomateriais) - Universidade Franciscana, Santa Maria, 2012. |
dc.identifier.uri.fl_str_mv |
http://tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/197 http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/289 |
identifier_str_mv |
OLIVEIRA, Amanda Lima de. EFEITO DAS β-CICLODEXTRINAS SOBRE PARÂMETROS BIOQUÍMICOS, DO METABOLISMO ENERGÉTICO E DO ESTRESSE OXIDATIVO EM RATOS WISTAR. 2012. 55 f. Dissertação (Mestrado em Biociências e Nanomateriais) - Universidade Franciscana, Santa Maria, 2012. |
url |
http://tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/197 http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/289 |
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Universidade Franciscana |
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Mestrado Acadêmico em Nanociências |
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Biociências e Nanomateriais |
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