DESENVOLVIMENTO E ESTUDO DE BIOCOMPATIBILIDADE IN VITRO DE NANOPARTÍCULAS LIPÍDICAS SÓLIDAS DE SEGUNDA GERAÇÃO À BASE DE MANTEIGA DE TUCUMÃ

• Autor(a) principal: Silveira, Larissa da Silva
• Data de Publicação: 2020
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional Universidade Franciscana
• Texto Completo: http://www.tede.universidadefranciscana.edu.br:8080/handle/UFN-BDTD/942
• topic: Astrocaryum vulgare, Chlorella homosphaera, Nanocarreadores lipídicos; Astrocaryum vulgare, Chlorella homosphaera, Lipid nanocarriers; Interdisciplinar

The ancient use of natural plants as a therapeutic alternative is increasingly common in the current reality of civilization where the use of natural bioactive agents for medicinal purposes, for the treatment, cure and prevention of diseases originates several studies that investigate the properties of assets from plants, flowers and fruits, which in addition to having almost no cytotoxic effect in most cases are sustainable. Currently, the major bet of the industries is the association of natural compounds with nanotechnology, which in addition to potentiating the action, ends up protecting the asset from various interferences such as rapid degradation. Currently, one of the focuses revolves around antimicrobial resistance, in view of the great need for new therapies capable of combating resistant microorganisms, without harming the patient and the environment. The present study aimed to prepare lipid nanocarriers (CLN), derived from the association of tucumã butter with grape seed oil and microalgal oil using the high-speed homogenization technique in the Ultra Turrax® device, characterizing and assessing stability against pH, zeta potential, polydispersity index and size, in addition to assessing rates of hemocompatibility, pharmacological safety, healing activity, antimicrobial and antibiofilm. Both formulations showed satisfactory stability for 60 days in different storage locations (presence of light, light shelter, greenhouse and refrigerator), the nanocarrier containing microalgae oil showed excellent antimicrobial activity with growth inhibition for 10 tested bacteria and antibiofilm activity for 8 of a total of 10 analyzes. The nanocarrier containing grape seed oil also showed antimicrobial activity, however, for a smaller number of isolates. The formulation containing grape seed oil, showed good efficacy for preventing, reversing and treating damage against hydrogen peroxide.