Understanding global changes of the liver proteome during murine schistosomiasis using a label-free shotgun approach.

• Autor(a) principal: Campos, Jonatan Marques
• Data de Publicação: 2016
• Outros Autores: Neves, Leandro Xavier, Paiva, Nívia Carolina Nogueira de, Castro, Renata Alves de Oliveira e, Casé, Ana Helena, Carneiro, Cláudia Martins, Andrade, Milton Hércules Guerra de, Borges, William de Castro
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFOP
• Texto Completo: http://www.repositorio.ufop.br/handle/123456789/9228; https://doi.org/10.1016/j.jprot.2016.07.013
• topic: Schistosoma mansoni; Liver biomarkers; Shotgun proteomics

Schistosomiasis is an endemic disease affecting over 207million people worldwide caused by helminth parasites of the genus Schistosoma. In Brazil the disease is responsible for the loss of up to 800 lives annually, resulting from the desabilitating effects of this chronic condition. In this study, we infected Balb/c mice with Schistosoma mansoni and analysed global changes in the proteomic profile of soluble liver proteins. Our shotgun analyses revealed predominance of up-regulation of proteins at 5weeks of infection, coincidingwith the onset of egg laying, and a remarkable down-regulation of liver constituents at 7 weeks, when severe tissue damage is installed. Representatives of glycolytic enzymes and stress response (in particular at the endoplasmic reticulum)were among the most differentially expressed molecules found in the infected liver. Collectively, our data contribute over 70 molecules not previously reported to be found at altered levels in murine schistosomiasis to further exploration of their potential as biomarkers of the disease.Moreover, understanding their intricate interaction using bioinformatics approach can potentially bring clarity to unknownmechanisms linked to the establishment of this condition in the vertebrate host. Significance: To our knowledge, this study refers to the first shotgun proteomic analysis to provide an inventory of the global changes in the liver soluble proteome caused by Schistosomamansoni in the Balb/cmodel. It also innovates by yielding data on quantification of the identified molecules as a manner to clarify and give insights into the underlying mechanisms for establishment of Schistosomiasis, a neglected tropical disease with historical prevalence in Brazil.