Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart.
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/4495 http://www.sciencedirect.com/science/article/pii/S016701151200002X |
Resumo: | In this study we investigated the effects of genetic deletion of the Angiotensin-(1-7) receptor Mas or the Angiotensin II receptor AT2 on the expression of specific extracellular matrix (ECM) proteins in atria, right ventricles and atrioventricular (AV) valves of neonatal and adult mice. Quantification of collagen types I, III and VI and fibronectin was performed using immunofluorescence-labeling and confocal microscopy. Picrosirius red staining was used for the histological assessment of the overall collagen distribution pattern. ECM proteins, metalloproteinases (MMP), ERK1/2 and p38 levels were quantified by western blot analysis. Gelatin zymography was used to evaluate the activity ofMMP-2 andMMP-9. We observed that the relative levels of collagen types I and III and fibronectin are significantly higher in both the right ventricle and AV valves of neonatal Mas−/− mouse hearts (e.g., collagen type I: 85.28±6.66 vs 43.50±4.41 arbitrary units in the right ventricles of Mas+/+ mice). Conversely, the level of collagen type VI was lower in the right ventricle and AV valves of Mas−/− mice. Adult Mas−/− mouse hearts presented similar patterns as observed in neonates. No significant differences in ECMprotein level were detected in atria. Likewise, no changes in ECM levels were observed in AT2 knockout mouse hearts. Although deletion of Mas induced a significant reduction in the level of the active form of MMP-2 in neonate hearts and a reduction of both MMP-2 and MMP-9 in adult Mas−/− mice, no significant differences were observed inMMP enzymatic activities when compared to controls. The levels of the active, phosphorylated forms of ERK1/2 and p38 were higher in hearts of both neonatal and adult Mas−/− mice. These observations suggest that Mas is involved in the selective expression of specific ECMproteins within both the ventricular myocardium and AV valves. The changes in the ECM profile may alter the connective tissue framework and contribute to the decreased cardiac performance observed in Mas−/− mice. |
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Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart.AngiotensinRenin-angiotensin systemFibrosisConnective tissueExtracellular matrixIn this study we investigated the effects of genetic deletion of the Angiotensin-(1-7) receptor Mas or the Angiotensin II receptor AT2 on the expression of specific extracellular matrix (ECM) proteins in atria, right ventricles and atrioventricular (AV) valves of neonatal and adult mice. Quantification of collagen types I, III and VI and fibronectin was performed using immunofluorescence-labeling and confocal microscopy. Picrosirius red staining was used for the histological assessment of the overall collagen distribution pattern. ECM proteins, metalloproteinases (MMP), ERK1/2 and p38 levels were quantified by western blot analysis. Gelatin zymography was used to evaluate the activity ofMMP-2 andMMP-9. We observed that the relative levels of collagen types I and III and fibronectin are significantly higher in both the right ventricle and AV valves of neonatal Mas−/− mouse hearts (e.g., collagen type I: 85.28±6.66 vs 43.50±4.41 arbitrary units in the right ventricles of Mas+/+ mice). Conversely, the level of collagen type VI was lower in the right ventricle and AV valves of Mas−/− mice. Adult Mas−/− mouse hearts presented similar patterns as observed in neonates. No significant differences in ECMprotein level were detected in atria. Likewise, no changes in ECM levels were observed in AT2 knockout mouse hearts. Although deletion of Mas induced a significant reduction in the level of the active form of MMP-2 in neonate hearts and a reduction of both MMP-2 and MMP-9 in adult Mas−/− mice, no significant differences were observed inMMP enzymatic activities when compared to controls. The levels of the active, phosphorylated forms of ERK1/2 and p38 were higher in hearts of both neonatal and adult Mas−/− mice. These observations suggest that Mas is involved in the selective expression of specific ECMproteins within both the ventricular myocardium and AV valves. The changes in the ECM profile may alter the connective tissue framework and contribute to the decreased cardiac performance observed in Mas−/− mice.2015-02-25T18:58:50Z2015-02-25T18:58:50Z2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfGAVA, E. et al. Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart. Regulatory Peptides, v. 175, p. 30-42, 2012. Disponível em: <http://www.sciencedirect.com/science/article/pii/S016701151200002X>. Acesso em: 08 nov. 2014.0167-0115http://www.repositorio.ufop.br/handle/123456789/4495http://www.sciencedirect.com/science/article/pii/S016701151200002XO periódico Regulatory Peptides concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3524850769887.info:eu-repo/semantics/openAccessGava, ElisandraCastro, Carlos Henrique deFerreira, Anderson JoséColleta, HeloísaMelo, Marcos BarrouinAlenina, NataliaBader, MichaelOliveira, Laser Antônio Machado deSantos, Robson Augusto Souza dosengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2019-06-24T12:55:44Zoai:repositorio.ufop.br:123456789/4495Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-06-24T12:55:44Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.none.fl_str_mv |
Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart. |
title |
Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart. |
spellingShingle |
Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart. Gava, Elisandra Angiotensin Renin-angiotensin system Fibrosis Connective tissue Extracellular matrix |
title_short |
Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart. |
title_full |
Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart. |
title_fullStr |
Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart. |
title_full_unstemmed |
Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart. |
title_sort |
Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart. |
author |
Gava, Elisandra |
author_facet |
Gava, Elisandra Castro, Carlos Henrique de Ferreira, Anderson José Colleta, Heloísa Melo, Marcos Barrouin Alenina, Natalia Bader, Michael Oliveira, Laser Antônio Machado de Santos, Robson Augusto Souza dos |
author_role |
author |
author2 |
Castro, Carlos Henrique de Ferreira, Anderson José Colleta, Heloísa Melo, Marcos Barrouin Alenina, Natalia Bader, Michael Oliveira, Laser Antônio Machado de Santos, Robson Augusto Souza dos |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Gava, Elisandra Castro, Carlos Henrique de Ferreira, Anderson José Colleta, Heloísa Melo, Marcos Barrouin Alenina, Natalia Bader, Michael Oliveira, Laser Antônio Machado de Santos, Robson Augusto Souza dos |
dc.subject.por.fl_str_mv |
Angiotensin Renin-angiotensin system Fibrosis Connective tissue Extracellular matrix |
topic |
Angiotensin Renin-angiotensin system Fibrosis Connective tissue Extracellular matrix |
description |
In this study we investigated the effects of genetic deletion of the Angiotensin-(1-7) receptor Mas or the Angiotensin II receptor AT2 on the expression of specific extracellular matrix (ECM) proteins in atria, right ventricles and atrioventricular (AV) valves of neonatal and adult mice. Quantification of collagen types I, III and VI and fibronectin was performed using immunofluorescence-labeling and confocal microscopy. Picrosirius red staining was used for the histological assessment of the overall collagen distribution pattern. ECM proteins, metalloproteinases (MMP), ERK1/2 and p38 levels were quantified by western blot analysis. Gelatin zymography was used to evaluate the activity ofMMP-2 andMMP-9. We observed that the relative levels of collagen types I and III and fibronectin are significantly higher in both the right ventricle and AV valves of neonatal Mas−/− mouse hearts (e.g., collagen type I: 85.28±6.66 vs 43.50±4.41 arbitrary units in the right ventricles of Mas+/+ mice). Conversely, the level of collagen type VI was lower in the right ventricle and AV valves of Mas−/− mice. Adult Mas−/− mouse hearts presented similar patterns as observed in neonates. No significant differences in ECMprotein level were detected in atria. Likewise, no changes in ECM levels were observed in AT2 knockout mouse hearts. Although deletion of Mas induced a significant reduction in the level of the active form of MMP-2 in neonate hearts and a reduction of both MMP-2 and MMP-9 in adult Mas−/− mice, no significant differences were observed inMMP enzymatic activities when compared to controls. The levels of the active, phosphorylated forms of ERK1/2 and p38 were higher in hearts of both neonatal and adult Mas−/− mice. These observations suggest that Mas is involved in the selective expression of specific ECMproteins within both the ventricular myocardium and AV valves. The changes in the ECM profile may alter the connective tissue framework and contribute to the decreased cardiac performance observed in Mas−/− mice. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 2015-02-25T18:58:50Z 2015-02-25T18:58:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
GAVA, E. et al. Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart. Regulatory Peptides, v. 175, p. 30-42, 2012. Disponível em: <http://www.sciencedirect.com/science/article/pii/S016701151200002X>. Acesso em: 08 nov. 2014. 0167-0115 http://www.repositorio.ufop.br/handle/123456789/4495 http://www.sciencedirect.com/science/article/pii/S016701151200002X |
identifier_str_mv |
GAVA, E. et al. Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart. Regulatory Peptides, v. 175, p. 30-42, 2012. Disponível em: <http://www.sciencedirect.com/science/article/pii/S016701151200002X>. Acesso em: 08 nov. 2014. 0167-0115 |
url |
http://www.repositorio.ufop.br/handle/123456789/4495 http://www.sciencedirect.com/science/article/pii/S016701151200002X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
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reponame:Repositório Institucional da UFOP instname:Universidade Federal de Ouro Preto (UFOP) instacron:UFOP |
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Universidade Federal de Ouro Preto (UFOP) |
instacron_str |
UFOP |
institution |
UFOP |
reponame_str |
Repositório Institucional da UFOP |
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Repositório Institucional da UFOP |
repository.name.fl_str_mv |
Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP) |
repository.mail.fl_str_mv |
repositorio@ufop.edu.br |
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1813002795629936640 |