Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart.

Detalhes bibliográficos
Autor(a) principal: Gava, Elisandra
Data de Publicação: 2012
Outros Autores: Castro, Carlos Henrique de, Ferreira, Anderson José, Colleta, Heloísa, Melo, Marcos Barrouin, Alenina, Natalia, Bader, Michael, Oliveira, Laser Antônio Machado de, Santos, Robson Augusto Souza dos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFOP
Texto Completo: http://www.repositorio.ufop.br/handle/123456789/4495
http://www.sciencedirect.com/science/article/pii/S016701151200002X
Resumo: In this study we investigated the effects of genetic deletion of the Angiotensin-(1-7) receptor Mas or the Angiotensin II receptor AT2 on the expression of specific extracellular matrix (ECM) proteins in atria, right ventricles and atrioventricular (AV) valves of neonatal and adult mice. Quantification of collagen types I, III and VI and fibronectin was performed using immunofluorescence-labeling and confocal microscopy. Picrosirius red staining was used for the histological assessment of the overall collagen distribution pattern. ECM proteins, metalloproteinases (MMP), ERK1/2 and p38 levels were quantified by western blot analysis. Gelatin zymography was used to evaluate the activity ofMMP-2 andMMP-9. We observed that the relative levels of collagen types I and III and fibronectin are significantly higher in both the right ventricle and AV valves of neonatal Mas−/− mouse hearts (e.g., collagen type I: 85.28±6.66 vs 43.50±4.41 arbitrary units in the right ventricles of Mas+/+ mice). Conversely, the level of collagen type VI was lower in the right ventricle and AV valves of Mas−/− mice. Adult Mas−/− mouse hearts presented similar patterns as observed in neonates. No significant differences in ECMprotein level were detected in atria. Likewise, no changes in ECM levels were observed in AT2 knockout mouse hearts. Although deletion of Mas induced a significant reduction in the level of the active form of MMP-2 in neonate hearts and a reduction of both MMP-2 and MMP-9 in adult Mas−/− mice, no significant differences were observed inMMP enzymatic activities when compared to controls. The levels of the active, phosphorylated forms of ERK1/2 and p38 were higher in hearts of both neonatal and adult Mas−/− mice. These observations suggest that Mas is involved in the selective expression of specific ECMproteins within both the ventricular myocardium and AV valves. The changes in the ECM profile may alter the connective tissue framework and contribute to the decreased cardiac performance observed in Mas−/− mice.
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spelling Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart.AngiotensinRenin-angiotensin systemFibrosisConnective tissueExtracellular matrixIn this study we investigated the effects of genetic deletion of the Angiotensin-(1-7) receptor Mas or the Angiotensin II receptor AT2 on the expression of specific extracellular matrix (ECM) proteins in atria, right ventricles and atrioventricular (AV) valves of neonatal and adult mice. Quantification of collagen types I, III and VI and fibronectin was performed using immunofluorescence-labeling and confocal microscopy. Picrosirius red staining was used for the histological assessment of the overall collagen distribution pattern. ECM proteins, metalloproteinases (MMP), ERK1/2 and p38 levels were quantified by western blot analysis. Gelatin zymography was used to evaluate the activity ofMMP-2 andMMP-9. We observed that the relative levels of collagen types I and III and fibronectin are significantly higher in both the right ventricle and AV valves of neonatal Mas−/− mouse hearts (e.g., collagen type I: 85.28±6.66 vs 43.50±4.41 arbitrary units in the right ventricles of Mas+/+ mice). Conversely, the level of collagen type VI was lower in the right ventricle and AV valves of Mas−/− mice. Adult Mas−/− mouse hearts presented similar patterns as observed in neonates. No significant differences in ECMprotein level were detected in atria. Likewise, no changes in ECM levels were observed in AT2 knockout mouse hearts. Although deletion of Mas induced a significant reduction in the level of the active form of MMP-2 in neonate hearts and a reduction of both MMP-2 and MMP-9 in adult Mas−/− mice, no significant differences were observed inMMP enzymatic activities when compared to controls. The levels of the active, phosphorylated forms of ERK1/2 and p38 were higher in hearts of both neonatal and adult Mas−/− mice. These observations suggest that Mas is involved in the selective expression of specific ECMproteins within both the ventricular myocardium and AV valves. The changes in the ECM profile may alter the connective tissue framework and contribute to the decreased cardiac performance observed in Mas−/− mice.2015-02-25T18:58:50Z2015-02-25T18:58:50Z2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfGAVA, E. et al. Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart. Regulatory Peptides, v. 175, p. 30-42, 2012. Disponível em: <http://www.sciencedirect.com/science/article/pii/S016701151200002X>. Acesso em: 08 nov. 2014.0167-0115http://www.repositorio.ufop.br/handle/123456789/4495http://www.sciencedirect.com/science/article/pii/S016701151200002XO periódico Regulatory Peptides concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3524850769887.info:eu-repo/semantics/openAccessGava, ElisandraCastro, Carlos Henrique deFerreira, Anderson JoséColleta, HeloísaMelo, Marcos BarrouinAlenina, NataliaBader, MichaelOliveira, Laser Antônio Machado deSantos, Robson Augusto Souza dosengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2019-06-24T12:55:44Zoai:repositorio.ufop.br:123456789/4495Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-06-24T12:55:44Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false
dc.title.none.fl_str_mv Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart.
title Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart.
spellingShingle Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart.
Gava, Elisandra
Angiotensin
Renin-angiotensin system
Fibrosis
Connective tissue
Extracellular matrix
title_short Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart.
title_full Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart.
title_fullStr Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart.
title_full_unstemmed Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart.
title_sort Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart.
author Gava, Elisandra
author_facet Gava, Elisandra
Castro, Carlos Henrique de
Ferreira, Anderson José
Colleta, Heloísa
Melo, Marcos Barrouin
Alenina, Natalia
Bader, Michael
Oliveira, Laser Antônio Machado de
Santos, Robson Augusto Souza dos
author_role author
author2 Castro, Carlos Henrique de
Ferreira, Anderson José
Colleta, Heloísa
Melo, Marcos Barrouin
Alenina, Natalia
Bader, Michael
Oliveira, Laser Antônio Machado de
Santos, Robson Augusto Souza dos
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gava, Elisandra
Castro, Carlos Henrique de
Ferreira, Anderson José
Colleta, Heloísa
Melo, Marcos Barrouin
Alenina, Natalia
Bader, Michael
Oliveira, Laser Antônio Machado de
Santos, Robson Augusto Souza dos
dc.subject.por.fl_str_mv Angiotensin
Renin-angiotensin system
Fibrosis
Connective tissue
Extracellular matrix
topic Angiotensin
Renin-angiotensin system
Fibrosis
Connective tissue
Extracellular matrix
description In this study we investigated the effects of genetic deletion of the Angiotensin-(1-7) receptor Mas or the Angiotensin II receptor AT2 on the expression of specific extracellular matrix (ECM) proteins in atria, right ventricles and atrioventricular (AV) valves of neonatal and adult mice. Quantification of collagen types I, III and VI and fibronectin was performed using immunofluorescence-labeling and confocal microscopy. Picrosirius red staining was used for the histological assessment of the overall collagen distribution pattern. ECM proteins, metalloproteinases (MMP), ERK1/2 and p38 levels were quantified by western blot analysis. Gelatin zymography was used to evaluate the activity ofMMP-2 andMMP-9. We observed that the relative levels of collagen types I and III and fibronectin are significantly higher in both the right ventricle and AV valves of neonatal Mas−/− mouse hearts (e.g., collagen type I: 85.28±6.66 vs 43.50±4.41 arbitrary units in the right ventricles of Mas+/+ mice). Conversely, the level of collagen type VI was lower in the right ventricle and AV valves of Mas−/− mice. Adult Mas−/− mouse hearts presented similar patterns as observed in neonates. No significant differences in ECMprotein level were detected in atria. Likewise, no changes in ECM levels were observed in AT2 knockout mouse hearts. Although deletion of Mas induced a significant reduction in the level of the active form of MMP-2 in neonate hearts and a reduction of both MMP-2 and MMP-9 in adult Mas−/− mice, no significant differences were observed inMMP enzymatic activities when compared to controls. The levels of the active, phosphorylated forms of ERK1/2 and p38 were higher in hearts of both neonatal and adult Mas−/− mice. These observations suggest that Mas is involved in the selective expression of specific ECMproteins within both the ventricular myocardium and AV valves. The changes in the ECM profile may alter the connective tissue framework and contribute to the decreased cardiac performance observed in Mas−/− mice.
publishDate 2012
dc.date.none.fl_str_mv 2012
2015-02-25T18:58:50Z
2015-02-25T18:58:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv GAVA, E. et al. Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart. Regulatory Peptides, v. 175, p. 30-42, 2012. Disponível em: <http://www.sciencedirect.com/science/article/pii/S016701151200002X>. Acesso em: 08 nov. 2014.
0167-0115
http://www.repositorio.ufop.br/handle/123456789/4495
http://www.sciencedirect.com/science/article/pii/S016701151200002X
identifier_str_mv GAVA, E. et al. Angiotensin-(1-7) receptor Mas is an essential modulator of extracellular matrix protein expression in the heart. Regulatory Peptides, v. 175, p. 30-42, 2012. Disponível em: <http://www.sciencedirect.com/science/article/pii/S016701151200002X>. Acesso em: 08 nov. 2014.
0167-0115
url http://www.repositorio.ufop.br/handle/123456789/4495
http://www.sciencedirect.com/science/article/pii/S016701151200002X
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFOP
instname:Universidade Federal de Ouro Preto (UFOP)
instacron:UFOP
instname_str Universidade Federal de Ouro Preto (UFOP)
instacron_str UFOP
institution UFOP
reponame_str Repositório Institucional da UFOP
collection Repositório Institucional da UFOP
repository.name.fl_str_mv Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)
repository.mail.fl_str_mv repositorio@ufop.edu.br
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