Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) delays egg hatching and larval development of progeny.
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/12002 https://doi.org/10.1093/jme/tjz110 |
Resumo: | Zika virus (ZIKV) has emerged as a globally important arbovirus and has been reported from all states of Brazil. The virus is primarily transmitted to humans through the bite of an infective Aedes aegypti (Linnaeus, 1762) or Aedes albopictus (Skuse, 1895). However, it is important to know if ZIKV transmission also occurs from Ae. aegypti through infected eggs to her offspring. Therefore, a ZIKV and dengue virus (DENV) free colony was established from eggs collected in Manaus and maintained until the third–fourth generation in order to conduct ZIKV vertical transmission (VT) experiments which used an infectious bloodmeal as the route of virus exposure. The eggs from ZIKV-infected females were allowed to hatch. The resulting F1 progeny (larvae, pupae, and adults) were quantitative polymerase chain reaction (qPCR) assayed for ZIKV. The viability of ZIKV vertically transmitted to F1 progeny was evaluated by cultivation in C6/36 cells. The effects of ZIKV on immature development of Ae. aegypti was assessed and compared with noninfected mosquitoes. Amazonian Ae. Aegypti were highly susceptible to ZIKV infection (96.7%), and viable virus passed to their progeny via VT. Moreover, eggs from the ZIKV-infected mosquitoes had a significantly lower hatch rate and the slowest hatching. In addition, the larval development period was slower when compared to noninfected, control mosquitoes. This is the first study to illustrate VT initiated by oral infection of the parental population by using mosquitoes, which originated from the field and a ZIKV strain that is naturally circulating in-country. Additionally, this study suggests that ZIKV present in the Ae. aegypti can modify the mosquito life cycle. The data reported here suggest that VT of ZIKV to progeny from naturally infected females may have a critical epidemiological role in the dissemination and maintenance of the virus circulating in the vector. |
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Chaves, Bárbara AparecidaVieira Junior, Ademir BentesSilveira, Karine Renata DiasPaz, Andréia da CostaVaz, Evelyn Beatriz da CostaAraújo, Raphaela Guedes PereiraRodrigues, Nilton BarnabéCampolina, Thaís BonifácioOrfanó, Alessandra da SilvaPimenta, Rafael NacifVillegas, Luis Eduardo MartínezMelo, Fabrício Freire deSilva, Breno de MelloMonteiro, Wuelton MarceloGuerra, Maria das Graças Vale BarbosaLacerda, Marcus Vinícius Guimarães deNorris, Douglas EricSecundino, Nagila Francinete CostaPimenta, Paulo Filemon Paolucci2020-03-24T15:46:55Z2020-03-24T15:46:55Z2019CHAVES, B. A. et al. Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) delays egg hatching and larval development of progeny. Journal of Medical Entomology, v. 56, n. 6, p. 1739–1744, nov. 2019. Disponível em: <https://academic.oup.com/jme/article/56/6/1739/5529105>. Acesso em: 10 fev. 2020.1938-2928http://www.repositorio.ufop.br/handle/123456789/12002https://doi.org/10.1093/jme/tjz110Zika virus (ZIKV) has emerged as a globally important arbovirus and has been reported from all states of Brazil. The virus is primarily transmitted to humans through the bite of an infective Aedes aegypti (Linnaeus, 1762) or Aedes albopictus (Skuse, 1895). However, it is important to know if ZIKV transmission also occurs from Ae. aegypti through infected eggs to her offspring. Therefore, a ZIKV and dengue virus (DENV) free colony was established from eggs collected in Manaus and maintained until the third–fourth generation in order to conduct ZIKV vertical transmission (VT) experiments which used an infectious bloodmeal as the route of virus exposure. The eggs from ZIKV-infected females were allowed to hatch. The resulting F1 progeny (larvae, pupae, and adults) were quantitative polymerase chain reaction (qPCR) assayed for ZIKV. The viability of ZIKV vertically transmitted to F1 progeny was evaluated by cultivation in C6/36 cells. The effects of ZIKV on immature development of Ae. aegypti was assessed and compared with noninfected mosquitoes. Amazonian Ae. Aegypti were highly susceptible to ZIKV infection (96.7%), and viable virus passed to their progeny via VT. Moreover, eggs from the ZIKV-infected mosquitoes had a significantly lower hatch rate and the slowest hatching. In addition, the larval development period was slower when compared to noninfected, control mosquitoes. This is the first study to illustrate VT initiated by oral infection of the parental population by using mosquitoes, which originated from the field and a ZIKV strain that is naturally circulating in-country. Additionally, this study suggests that ZIKV present in the Ae. aegypti can modify the mosquito life cycle. The data reported here suggest that VT of ZIKV to progeny from naturally infected females may have a critical epidemiological role in the dissemination and maintenance of the virus circulating in the vector.This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/ by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. Fonte: o próprio artigo.info:eu-repo/semantics/openAccessFitness costVertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) delays egg hatching and larval development of progeny.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-8924http://www.repositorio.ufop.br/bitstream/123456789/12002/2/license.txt62604f8d955274beb56c80ce1ee5dcaeMD52ORIGINALARTIGO_VerticalTransmissionZika.pdfARTIGO_VerticalTransmissionZika.pdfapplication/pdf3204563http://www.repositorio.ufop.br/bitstream/123456789/12002/1/ARTIGO_VerticalTransmissionZika.pdf956380197a3f7b41364c74308761f849MD51123456789/120022020-03-24 11:46:55.929oai:localhost: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ório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332020-03-24T15:46:55Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) delays egg hatching and larval development of progeny. |
title |
Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) delays egg hatching and larval development of progeny. |
spellingShingle |
Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) delays egg hatching and larval development of progeny. Chaves, Bárbara Aparecida Fitness cost |
title_short |
Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) delays egg hatching and larval development of progeny. |
title_full |
Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) delays egg hatching and larval development of progeny. |
title_fullStr |
Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) delays egg hatching and larval development of progeny. |
title_full_unstemmed |
Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) delays egg hatching and larval development of progeny. |
title_sort |
Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) delays egg hatching and larval development of progeny. |
author |
Chaves, Bárbara Aparecida |
author_facet |
Chaves, Bárbara Aparecida Vieira Junior, Ademir Bentes Silveira, Karine Renata Dias Paz, Andréia da Costa Vaz, Evelyn Beatriz da Costa Araújo, Raphaela Guedes Pereira Rodrigues, Nilton Barnabé Campolina, Thaís Bonifácio Orfanó, Alessandra da Silva Pimenta, Rafael Nacif Villegas, Luis Eduardo Martínez Melo, Fabrício Freire de Silva, Breno de Mello Monteiro, Wuelton Marcelo Guerra, Maria das Graças Vale Barbosa Lacerda, Marcus Vinícius Guimarães de Norris, Douglas Eric Secundino, Nagila Francinete Costa Pimenta, Paulo Filemon Paolucci |
author_role |
author |
author2 |
Vieira Junior, Ademir Bentes Silveira, Karine Renata Dias Paz, Andréia da Costa Vaz, Evelyn Beatriz da Costa Araújo, Raphaela Guedes Pereira Rodrigues, Nilton Barnabé Campolina, Thaís Bonifácio Orfanó, Alessandra da Silva Pimenta, Rafael Nacif Villegas, Luis Eduardo Martínez Melo, Fabrício Freire de Silva, Breno de Mello Monteiro, Wuelton Marcelo Guerra, Maria das Graças Vale Barbosa Lacerda, Marcus Vinícius Guimarães de Norris, Douglas Eric Secundino, Nagila Francinete Costa Pimenta, Paulo Filemon Paolucci |
author2_role |
author author author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Chaves, Bárbara Aparecida Vieira Junior, Ademir Bentes Silveira, Karine Renata Dias Paz, Andréia da Costa Vaz, Evelyn Beatriz da Costa Araújo, Raphaela Guedes Pereira Rodrigues, Nilton Barnabé Campolina, Thaís Bonifácio Orfanó, Alessandra da Silva Pimenta, Rafael Nacif Villegas, Luis Eduardo Martínez Melo, Fabrício Freire de Silva, Breno de Mello Monteiro, Wuelton Marcelo Guerra, Maria das Graças Vale Barbosa Lacerda, Marcus Vinícius Guimarães de Norris, Douglas Eric Secundino, Nagila Francinete Costa Pimenta, Paulo Filemon Paolucci |
dc.subject.por.fl_str_mv |
Fitness cost |
topic |
Fitness cost |
description |
Zika virus (ZIKV) has emerged as a globally important arbovirus and has been reported from all states of Brazil. The virus is primarily transmitted to humans through the bite of an infective Aedes aegypti (Linnaeus, 1762) or Aedes albopictus (Skuse, 1895). However, it is important to know if ZIKV transmission also occurs from Ae. aegypti through infected eggs to her offspring. Therefore, a ZIKV and dengue virus (DENV) free colony was established from eggs collected in Manaus and maintained until the third–fourth generation in order to conduct ZIKV vertical transmission (VT) experiments which used an infectious bloodmeal as the route of virus exposure. The eggs from ZIKV-infected females were allowed to hatch. The resulting F1 progeny (larvae, pupae, and adults) were quantitative polymerase chain reaction (qPCR) assayed for ZIKV. The viability of ZIKV vertically transmitted to F1 progeny was evaluated by cultivation in C6/36 cells. The effects of ZIKV on immature development of Ae. aegypti was assessed and compared with noninfected mosquitoes. Amazonian Ae. Aegypti were highly susceptible to ZIKV infection (96.7%), and viable virus passed to their progeny via VT. Moreover, eggs from the ZIKV-infected mosquitoes had a significantly lower hatch rate and the slowest hatching. In addition, the larval development period was slower when compared to noninfected, control mosquitoes. This is the first study to illustrate VT initiated by oral infection of the parental population by using mosquitoes, which originated from the field and a ZIKV strain that is naturally circulating in-country. Additionally, this study suggests that ZIKV present in the Ae. aegypti can modify the mosquito life cycle. The data reported here suggest that VT of ZIKV to progeny from naturally infected females may have a critical epidemiological role in the dissemination and maintenance of the virus circulating in the vector. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019 |
dc.date.accessioned.fl_str_mv |
2020-03-24T15:46:55Z |
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2020-03-24T15:46:55Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
CHAVES, B. A. et al. Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) delays egg hatching and larval development of progeny. Journal of Medical Entomology, v. 56, n. 6, p. 1739–1744, nov. 2019. Disponível em: <https://academic.oup.com/jme/article/56/6/1739/5529105>. Acesso em: 10 fev. 2020. |
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http://www.repositorio.ufop.br/handle/123456789/12002 |
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1938-2928 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1093/jme/tjz110 |
identifier_str_mv |
CHAVES, B. A. et al. Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) delays egg hatching and larval development of progeny. Journal of Medical Entomology, v. 56, n. 6, p. 1739–1744, nov. 2019. Disponível em: <https://academic.oup.com/jme/article/56/6/1739/5529105>. Acesso em: 10 fev. 2020. 1938-2928 |
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