Validation of a colorimetric assay for the in vitro screening of inhibitors of angiotensin-converting enzyme (ACE) from plant extracts.
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/1215 |
Resumo: | Anew method for the in vitro screening of plant extracts with potential angiotensin-converting enzyme (ACE) inhibitory activity is proposed. The method is based on the cleavage of the substrate hippuryl-glycyl-glycine by ACE and subsequent reaction with trinitrobenzenesulfonic acid to form 2,4,6-trinitrophenyl-glycyl-glycine, whose absorbance is determined at 415nm in a microtitre plate reader. Rabbit lung dehydrated by acetone was employed as an enzyme source. Validation of the method showed satisfactory intra-day (CV ¼ 7.63%) and inter-day precision (CV ¼ 13.61%), recovery (97–102.1%), sensitivity (IC50 ¼ 14.1 nmol/l) and linearity in the range 7.5–120 mmol/l of glycyl-glycine (r2 ¼ 0:9921). Besides, the method showed good correlation with a HPLC assay already established for the screening of ACE inhibitors (r ¼ 0:9935 and 0:9034; respectively, for captopril solutions and for plant extracts). The method involves only inexpensive reagents and apparatus. |
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Serra, Carla PenidoCôrtes, Steyner de FrançaLombardi, Júlio AntônioOliveira, Alaíde Braga deBraga, Fernão Castro2012-07-30T14:24:28Z2012-07-30T14:24:28Z2005SERRA, C. P. et al. Validation of a colorimetric assay for the in vitro screening of inhibitors of angiotensin-converting enzyme (ACE) from plant extracts. Phytomedicine, v. 12, n. 6-7, p. 424-432, jun. 2005. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0944711305000590>. Acesso em: 30 jul. 2012.09447113http://www.repositorio.ufop.br/handle/123456789/1215Anew method for the in vitro screening of plant extracts with potential angiotensin-converting enzyme (ACE) inhibitory activity is proposed. The method is based on the cleavage of the substrate hippuryl-glycyl-glycine by ACE and subsequent reaction with trinitrobenzenesulfonic acid to form 2,4,6-trinitrophenyl-glycyl-glycine, whose absorbance is determined at 415nm in a microtitre plate reader. Rabbit lung dehydrated by acetone was employed as an enzyme source. Validation of the method showed satisfactory intra-day (CV ¼ 7.63%) and inter-day precision (CV ¼ 13.61%), recovery (97–102.1%), sensitivity (IC50 ¼ 14.1 nmol/l) and linearity in the range 7.5–120 mmol/l of glycyl-glycine (r2 ¼ 0:9921). Besides, the method showed good correlation with a HPLC assay already established for the screening of ACE inhibitors (r ¼ 0:9935 and 0:9034; respectively, for captopril solutions and for plant extracts). The method involves only inexpensive reagents and apparatus.In vitro colorimetric assayPlant extractsScreeningAngiotensin-converting enzyme inhibitorsValidation of a colorimetric assay for the in vitro screening of inhibitors of angiotensin-converting enzyme (ACE) from plant extracts.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleO periódico Phytomedicine concede permissão ao repositório Instucional da UFOP para depositar este artigo. Número da licença: 3233050124767.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPORIGINALARTIGO_ValidationColorimetricAssay.pdfARTIGO_ValidationColorimetricAssay.pdfapplication/pdf218377http://www.repositorio.ufop.br/bitstream/123456789/1215/1/ARTIGO_ValidationColorimetricAssay.pdf12333e18f2ddbc19139cc28a0eac7930MD51123456789/12152019-02-28 10:11:38.848oai:localhost:123456789/1215Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-02-28T15:11:38Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Validation of a colorimetric assay for the in vitro screening of inhibitors of angiotensin-converting enzyme (ACE) from plant extracts. |
title |
Validation of a colorimetric assay for the in vitro screening of inhibitors of angiotensin-converting enzyme (ACE) from plant extracts. |
spellingShingle |
Validation of a colorimetric assay for the in vitro screening of inhibitors of angiotensin-converting enzyme (ACE) from plant extracts. Serra, Carla Penido In vitro colorimetric assay Plant extracts Screening Angiotensin-converting enzyme inhibitors |
title_short |
Validation of a colorimetric assay for the in vitro screening of inhibitors of angiotensin-converting enzyme (ACE) from plant extracts. |
title_full |
Validation of a colorimetric assay for the in vitro screening of inhibitors of angiotensin-converting enzyme (ACE) from plant extracts. |
title_fullStr |
Validation of a colorimetric assay for the in vitro screening of inhibitors of angiotensin-converting enzyme (ACE) from plant extracts. |
title_full_unstemmed |
Validation of a colorimetric assay for the in vitro screening of inhibitors of angiotensin-converting enzyme (ACE) from plant extracts. |
title_sort |
Validation of a colorimetric assay for the in vitro screening of inhibitors of angiotensin-converting enzyme (ACE) from plant extracts. |
author |
Serra, Carla Penido |
author_facet |
Serra, Carla Penido Côrtes, Steyner de França Lombardi, Júlio Antônio Oliveira, Alaíde Braga de Braga, Fernão Castro |
author_role |
author |
author2 |
Côrtes, Steyner de França Lombardi, Júlio Antônio Oliveira, Alaíde Braga de Braga, Fernão Castro |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Serra, Carla Penido Côrtes, Steyner de França Lombardi, Júlio Antônio Oliveira, Alaíde Braga de Braga, Fernão Castro |
dc.subject.por.fl_str_mv |
In vitro colorimetric assay Plant extracts Screening Angiotensin-converting enzyme inhibitors |
topic |
In vitro colorimetric assay Plant extracts Screening Angiotensin-converting enzyme inhibitors |
description |
Anew method for the in vitro screening of plant extracts with potential angiotensin-converting enzyme (ACE) inhibitory activity is proposed. The method is based on the cleavage of the substrate hippuryl-glycyl-glycine by ACE and subsequent reaction with trinitrobenzenesulfonic acid to form 2,4,6-trinitrophenyl-glycyl-glycine, whose absorbance is determined at 415nm in a microtitre plate reader. Rabbit lung dehydrated by acetone was employed as an enzyme source. Validation of the method showed satisfactory intra-day (CV ¼ 7.63%) and inter-day precision (CV ¼ 13.61%), recovery (97–102.1%), sensitivity (IC50 ¼ 14.1 nmol/l) and linearity in the range 7.5–120 mmol/l of glycyl-glycine (r2 ¼ 0:9921). Besides, the method showed good correlation with a HPLC assay already established for the screening of ACE inhibitors (r ¼ 0:9935 and 0:9034; respectively, for captopril solutions and for plant extracts). The method involves only inexpensive reagents and apparatus. |
publishDate |
2005 |
dc.date.issued.fl_str_mv |
2005 |
dc.date.accessioned.fl_str_mv |
2012-07-30T14:24:28Z |
dc.date.available.fl_str_mv |
2012-07-30T14:24:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SERRA, C. P. et al. Validation of a colorimetric assay for the in vitro screening of inhibitors of angiotensin-converting enzyme (ACE) from plant extracts. Phytomedicine, v. 12, n. 6-7, p. 424-432, jun. 2005. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0944711305000590>. Acesso em: 30 jul. 2012. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/1215 |
dc.identifier.issn.none.fl_str_mv |
09447113 |
identifier_str_mv |
SERRA, C. P. et al. Validation of a colorimetric assay for the in vitro screening of inhibitors of angiotensin-converting enzyme (ACE) from plant extracts. Phytomedicine, v. 12, n. 6-7, p. 424-432, jun. 2005. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0944711305000590>. Acesso em: 30 jul. 2012. 09447113 |
url |
http://www.repositorio.ufop.br/handle/123456789/1215 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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reponame:Repositório Institucional da UFOP instname:Universidade Federal de Ouro Preto (UFOP) instacron:UFOP |
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Universidade Federal de Ouro Preto (UFOP) |
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UFOP |
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UFOP |
reponame_str |
Repositório Institucional da UFOP |
collection |
Repositório Institucional da UFOP |
bitstream.url.fl_str_mv |
http://www.repositorio.ufop.br/bitstream/123456789/1215/1/ARTIGO_ValidationColorimetricAssay.pdf |
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Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP) |
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repositorio@ufop.edu.br |
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