Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats.

Detalhes bibliográficos
Autor(a) principal: Gonçalves, Reggiani Vilela
Data de Publicação: 2010
Outros Autores: Mezêncio, J. M. S., Benevides, Gustavo Pereira, Mata, Sérgio Luis Pinto da, Neves, Clóvis Andrade, Sarandy, Mariáurea Matias, Vilela, Evaldo Ferreira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFOP
Texto Completo: http://www.repositorio.ufop.br/handle/123456789/4498
http://www.scielo.br/pdf/bjmbr/v43n4/7707.pdf
Resumo: This study determined the effects of gallium-aluminum-arsenide laser (GaAlAs), gallium-arsenide laser (GaAs) and Dersani® healing ointment on skin wounds in Wistar rats. The parameters analyzed were: type I and III collagen fiber concentrations as well as the rate of wound closure. Five wounds, 12 mm in diameter, were made on the animals’ backs. The depth of the surgical incision was controlled by removing the epithelial tissue until the dorsal muscular fascia was exposed. The animals were anesthetized with ketamine and xylazine via intraperitoneal injection. The rats were randomly divided into five groups of 6 animals each, according to the treatment received. Group 1 (L4): GaAs laser (4 J/cm2); group 2 (L30): GaAlAs laser (30 J/cm2); group 3 (L60): GaAlAs laser (60 J/cm2); group 4 (D): Dersani® ointment; group 5 (control): 0.9% saline. The applications were made daily over a period of 20 days. Tissue fragments were stained with picrosirius to distinguish type I collagen from type III collagen. The collagen fibers were photo-documented and analyzed using the Quantum software based on the primary color spectrum (red, yellow and blue). Significant results for wound closing rate were obtained for group 1 (L4), 7.37 mm/day. The highest concentration of type III collagen fibers was observed in group 2 (L30; 37.80 ± 7.10%), which differed from control (29.86 ± 5.15%) on the 20th day of treatment. The type I collagen fibers of group 1 (L4; 2.67 ± 2.23%) and group 2 (L30; 2.87 ± 2.40%) differed significantly from control (1.77 ± 2.97%) on the 20th day of the experiment.
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spelling Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats.CicatrizationLaserHealing ointmentCollagenCutaneous woundThis study determined the effects of gallium-aluminum-arsenide laser (GaAlAs), gallium-arsenide laser (GaAs) and Dersani® healing ointment on skin wounds in Wistar rats. The parameters analyzed were: type I and III collagen fiber concentrations as well as the rate of wound closure. Five wounds, 12 mm in diameter, were made on the animals’ backs. The depth of the surgical incision was controlled by removing the epithelial tissue until the dorsal muscular fascia was exposed. The animals were anesthetized with ketamine and xylazine via intraperitoneal injection. The rats were randomly divided into five groups of 6 animals each, according to the treatment received. Group 1 (L4): GaAs laser (4 J/cm2); group 2 (L30): GaAlAs laser (30 J/cm2); group 3 (L60): GaAlAs laser (60 J/cm2); group 4 (D): Dersani® ointment; group 5 (control): 0.9% saline. The applications were made daily over a period of 20 days. Tissue fragments were stained with picrosirius to distinguish type I collagen from type III collagen. The collagen fibers were photo-documented and analyzed using the Quantum software based on the primary color spectrum (red, yellow and blue). Significant results for wound closing rate were obtained for group 1 (L4), 7.37 mm/day. The highest concentration of type III collagen fibers was observed in group 2 (L30; 37.80 ± 7.10%), which differed from control (29.86 ± 5.15%) on the 20th day of treatment. The type I collagen fibers of group 1 (L4; 2.67 ± 2.23%) and group 2 (L30; 2.87 ± 2.40%) differed significantly from control (1.77 ± 2.97%) on the 20th day of the experiment.2015-02-25T18:59:42Z2015-02-25T18:59:42Z2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfGONÇALVES, R. V. et al. Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats. Brazilian Journal of Medical and Biological Research, v. 43, p. 350-355, 2010. Disponível em: <http://www.scielo.br/pdf/bjmbr/v43n4/7707.pdf>. Acesso em: 08 nov. 2014.1414-431Xhttp://www.repositorio.ufop.br/handle/123456789/4498http://www.scielo.br/pdf/bjmbr/v43n4/7707.pdfNo permission is required from the authors or the publishers download, reuse, reprint, modify, distribute, and/or copy articles published in the BJMBR, as long as the original authors and source are cited. Fonte: Brazilian Journal of Medical and Biological Research <http://www.bjournal.com.br/index.php?option=com_content&view=article&id=185&Itemid=139>. Acesso em: 21 jan. 2014.info:eu-repo/semantics/openAccessGonçalves, Reggiani VilelaMezêncio, J. M. S.Benevides, Gustavo PereiraMata, Sérgio Luis Pinto daNeves, Clóvis AndradeSarandy, Mariáurea MatiasVilela, Evaldo Ferreiraengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2019-06-24T13:03:40Zoai:repositorio.ufop.br:123456789/4498Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-06-24T13:03:40Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false
dc.title.none.fl_str_mv Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats.
title Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats.
spellingShingle Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats.
Gonçalves, Reggiani Vilela
Cicatrization
Laser
Healing ointment
Collagen
Cutaneous wound
title_short Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats.
title_full Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats.
title_fullStr Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats.
title_full_unstemmed Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats.
title_sort Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats.
author Gonçalves, Reggiani Vilela
author_facet Gonçalves, Reggiani Vilela
Mezêncio, J. M. S.
Benevides, Gustavo Pereira
Mata, Sérgio Luis Pinto da
Neves, Clóvis Andrade
Sarandy, Mariáurea Matias
Vilela, Evaldo Ferreira
author_role author
author2 Mezêncio, J. M. S.
Benevides, Gustavo Pereira
Mata, Sérgio Luis Pinto da
Neves, Clóvis Andrade
Sarandy, Mariáurea Matias
Vilela, Evaldo Ferreira
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gonçalves, Reggiani Vilela
Mezêncio, J. M. S.
Benevides, Gustavo Pereira
Mata, Sérgio Luis Pinto da
Neves, Clóvis Andrade
Sarandy, Mariáurea Matias
Vilela, Evaldo Ferreira
dc.subject.por.fl_str_mv Cicatrization
Laser
Healing ointment
Collagen
Cutaneous wound
topic Cicatrization
Laser
Healing ointment
Collagen
Cutaneous wound
description This study determined the effects of gallium-aluminum-arsenide laser (GaAlAs), gallium-arsenide laser (GaAs) and Dersani® healing ointment on skin wounds in Wistar rats. The parameters analyzed were: type I and III collagen fiber concentrations as well as the rate of wound closure. Five wounds, 12 mm in diameter, were made on the animals’ backs. The depth of the surgical incision was controlled by removing the epithelial tissue until the dorsal muscular fascia was exposed. The animals were anesthetized with ketamine and xylazine via intraperitoneal injection. The rats were randomly divided into five groups of 6 animals each, according to the treatment received. Group 1 (L4): GaAs laser (4 J/cm2); group 2 (L30): GaAlAs laser (30 J/cm2); group 3 (L60): GaAlAs laser (60 J/cm2); group 4 (D): Dersani® ointment; group 5 (control): 0.9% saline. The applications were made daily over a period of 20 days. Tissue fragments were stained with picrosirius to distinguish type I collagen from type III collagen. The collagen fibers were photo-documented and analyzed using the Quantum software based on the primary color spectrum (red, yellow and blue). Significant results for wound closing rate were obtained for group 1 (L4), 7.37 mm/day. The highest concentration of type III collagen fibers was observed in group 2 (L30; 37.80 ± 7.10%), which differed from control (29.86 ± 5.15%) on the 20th day of treatment. The type I collagen fibers of group 1 (L4; 2.67 ± 2.23%) and group 2 (L30; 2.87 ± 2.40%) differed significantly from control (1.77 ± 2.97%) on the 20th day of the experiment.
publishDate 2010
dc.date.none.fl_str_mv 2010
2015-02-25T18:59:42Z
2015-02-25T18:59:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv GONÇALVES, R. V. et al. Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats. Brazilian Journal of Medical and Biological Research, v. 43, p. 350-355, 2010. Disponível em: <http://www.scielo.br/pdf/bjmbr/v43n4/7707.pdf>. Acesso em: 08 nov. 2014.
1414-431X
http://www.repositorio.ufop.br/handle/123456789/4498
http://www.scielo.br/pdf/bjmbr/v43n4/7707.pdf
identifier_str_mv GONÇALVES, R. V. et al. Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats. Brazilian Journal of Medical and Biological Research, v. 43, p. 350-355, 2010. Disponível em: <http://www.scielo.br/pdf/bjmbr/v43n4/7707.pdf>. Acesso em: 08 nov. 2014.
1414-431X
url http://www.repositorio.ufop.br/handle/123456789/4498
http://www.scielo.br/pdf/bjmbr/v43n4/7707.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFOP
instname:Universidade Federal de Ouro Preto (UFOP)
instacron:UFOP
instname_str Universidade Federal de Ouro Preto (UFOP)
instacron_str UFOP
institution UFOP
reponame_str Repositório Institucional da UFOP
collection Repositório Institucional da UFOP
repository.name.fl_str_mv Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)
repository.mail.fl_str_mv repositorio@ufop.edu.br
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