Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats.
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/4498 http://www.scielo.br/pdf/bjmbr/v43n4/7707.pdf |
Resumo: | This study determined the effects of gallium-aluminum-arsenide laser (GaAlAs), gallium-arsenide laser (GaAs) and Dersani® healing ointment on skin wounds in Wistar rats. The parameters analyzed were: type I and III collagen fiber concentrations as well as the rate of wound closure. Five wounds, 12 mm in diameter, were made on the animals’ backs. The depth of the surgical incision was controlled by removing the epithelial tissue until the dorsal muscular fascia was exposed. The animals were anesthetized with ketamine and xylazine via intraperitoneal injection. The rats were randomly divided into five groups of 6 animals each, according to the treatment received. Group 1 (L4): GaAs laser (4 J/cm2); group 2 (L30): GaAlAs laser (30 J/cm2); group 3 (L60): GaAlAs laser (60 J/cm2); group 4 (D): Dersani® ointment; group 5 (control): 0.9% saline. The applications were made daily over a period of 20 days. Tissue fragments were stained with picrosirius to distinguish type I collagen from type III collagen. The collagen fibers were photo-documented and analyzed using the Quantum software based on the primary color spectrum (red, yellow and blue). Significant results for wound closing rate were obtained for group 1 (L4), 7.37 mm/day. The highest concentration of type III collagen fibers was observed in group 2 (L30; 37.80 ± 7.10%), which differed from control (29.86 ± 5.15%) on the 20th day of treatment. The type I collagen fibers of group 1 (L4; 2.67 ± 2.23%) and group 2 (L30; 2.87 ± 2.40%) differed significantly from control (1.77 ± 2.97%) on the 20th day of the experiment. |
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Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats.CicatrizationLaserHealing ointmentCollagenCutaneous woundThis study determined the effects of gallium-aluminum-arsenide laser (GaAlAs), gallium-arsenide laser (GaAs) and Dersani® healing ointment on skin wounds in Wistar rats. The parameters analyzed were: type I and III collagen fiber concentrations as well as the rate of wound closure. Five wounds, 12 mm in diameter, were made on the animals’ backs. The depth of the surgical incision was controlled by removing the epithelial tissue until the dorsal muscular fascia was exposed. The animals were anesthetized with ketamine and xylazine via intraperitoneal injection. The rats were randomly divided into five groups of 6 animals each, according to the treatment received. Group 1 (L4): GaAs laser (4 J/cm2); group 2 (L30): GaAlAs laser (30 J/cm2); group 3 (L60): GaAlAs laser (60 J/cm2); group 4 (D): Dersani® ointment; group 5 (control): 0.9% saline. The applications were made daily over a period of 20 days. Tissue fragments were stained with picrosirius to distinguish type I collagen from type III collagen. The collagen fibers were photo-documented and analyzed using the Quantum software based on the primary color spectrum (red, yellow and blue). Significant results for wound closing rate were obtained for group 1 (L4), 7.37 mm/day. The highest concentration of type III collagen fibers was observed in group 2 (L30; 37.80 ± 7.10%), which differed from control (29.86 ± 5.15%) on the 20th day of treatment. The type I collagen fibers of group 1 (L4; 2.67 ± 2.23%) and group 2 (L30; 2.87 ± 2.40%) differed significantly from control (1.77 ± 2.97%) on the 20th day of the experiment.2015-02-25T18:59:42Z2015-02-25T18:59:42Z2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfGONÇALVES, R. V. et al. Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats. Brazilian Journal of Medical and Biological Research, v. 43, p. 350-355, 2010. Disponível em: <http://www.scielo.br/pdf/bjmbr/v43n4/7707.pdf>. Acesso em: 08 nov. 2014.1414-431Xhttp://www.repositorio.ufop.br/handle/123456789/4498http://www.scielo.br/pdf/bjmbr/v43n4/7707.pdfNo permission is required from the authors or the publishers download, reuse, reprint, modify, distribute, and/or copy articles published in the BJMBR, as long as the original authors and source are cited. Fonte: Brazilian Journal of Medical and Biological Research <http://www.bjournal.com.br/index.php?option=com_content&view=article&id=185&Itemid=139>. Acesso em: 21 jan. 2014.info:eu-repo/semantics/openAccessGonçalves, Reggiani VilelaMezêncio, J. M. S.Benevides, Gustavo PereiraMata, Sérgio Luis Pinto daNeves, Clóvis AndradeSarandy, Mariáurea MatiasVilela, Evaldo Ferreiraengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2019-06-24T13:03:40Zoai:repositorio.ufop.br:123456789/4498Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-06-24T13:03:40Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.none.fl_str_mv |
Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats. |
title |
Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats. |
spellingShingle |
Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats. Gonçalves, Reggiani Vilela Cicatrization Laser Healing ointment Collagen Cutaneous wound |
title_short |
Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats. |
title_full |
Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats. |
title_fullStr |
Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats. |
title_full_unstemmed |
Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats. |
title_sort |
Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats. |
author |
Gonçalves, Reggiani Vilela |
author_facet |
Gonçalves, Reggiani Vilela Mezêncio, J. M. S. Benevides, Gustavo Pereira Mata, Sérgio Luis Pinto da Neves, Clóvis Andrade Sarandy, Mariáurea Matias Vilela, Evaldo Ferreira |
author_role |
author |
author2 |
Mezêncio, J. M. S. Benevides, Gustavo Pereira Mata, Sérgio Luis Pinto da Neves, Clóvis Andrade Sarandy, Mariáurea Matias Vilela, Evaldo Ferreira |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Gonçalves, Reggiani Vilela Mezêncio, J. M. S. Benevides, Gustavo Pereira Mata, Sérgio Luis Pinto da Neves, Clóvis Andrade Sarandy, Mariáurea Matias Vilela, Evaldo Ferreira |
dc.subject.por.fl_str_mv |
Cicatrization Laser Healing ointment Collagen Cutaneous wound |
topic |
Cicatrization Laser Healing ointment Collagen Cutaneous wound |
description |
This study determined the effects of gallium-aluminum-arsenide laser (GaAlAs), gallium-arsenide laser (GaAs) and Dersani® healing ointment on skin wounds in Wistar rats. The parameters analyzed were: type I and III collagen fiber concentrations as well as the rate of wound closure. Five wounds, 12 mm in diameter, were made on the animals’ backs. The depth of the surgical incision was controlled by removing the epithelial tissue until the dorsal muscular fascia was exposed. The animals were anesthetized with ketamine and xylazine via intraperitoneal injection. The rats were randomly divided into five groups of 6 animals each, according to the treatment received. Group 1 (L4): GaAs laser (4 J/cm2); group 2 (L30): GaAlAs laser (30 J/cm2); group 3 (L60): GaAlAs laser (60 J/cm2); group 4 (D): Dersani® ointment; group 5 (control): 0.9% saline. The applications were made daily over a period of 20 days. Tissue fragments were stained with picrosirius to distinguish type I collagen from type III collagen. The collagen fibers were photo-documented and analyzed using the Quantum software based on the primary color spectrum (red, yellow and blue). Significant results for wound closing rate were obtained for group 1 (L4), 7.37 mm/day. The highest concentration of type III collagen fibers was observed in group 2 (L30; 37.80 ± 7.10%), which differed from control (29.86 ± 5.15%) on the 20th day of treatment. The type I collagen fibers of group 1 (L4; 2.67 ± 2.23%) and group 2 (L30; 2.87 ± 2.40%) differed significantly from control (1.77 ± 2.97%) on the 20th day of the experiment. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 2015-02-25T18:59:42Z 2015-02-25T18:59:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
GONÇALVES, R. V. et al. Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats. Brazilian Journal of Medical and Biological Research, v. 43, p. 350-355, 2010. Disponível em: <http://www.scielo.br/pdf/bjmbr/v43n4/7707.pdf>. Acesso em: 08 nov. 2014. 1414-431X http://www.repositorio.ufop.br/handle/123456789/4498 http://www.scielo.br/pdf/bjmbr/v43n4/7707.pdf |
identifier_str_mv |
GONÇALVES, R. V. et al. Effect of gallium-arsenide laser, gallium aluminumarsenide laser and healing ointment on cutaneous wound healing in Wistar rats. Brazilian Journal of Medical and Biological Research, v. 43, p. 350-355, 2010. Disponível em: <http://www.scielo.br/pdf/bjmbr/v43n4/7707.pdf>. Acesso em: 08 nov. 2014. 1414-431X |
url |
http://www.repositorio.ufop.br/handle/123456789/4498 http://www.scielo.br/pdf/bjmbr/v43n4/7707.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFOP instname:Universidade Federal de Ouro Preto (UFOP) instacron:UFOP |
instname_str |
Universidade Federal de Ouro Preto (UFOP) |
instacron_str |
UFOP |
institution |
UFOP |
reponame_str |
Repositório Institucional da UFOP |
collection |
Repositório Institucional da UFOP |
repository.name.fl_str_mv |
Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP) |
repository.mail.fl_str_mv |
repositorio@ufop.edu.br |
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