Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis.

Detalhes bibliográficos
Autor(a) principal: Brito, Rory Cristiane Fortes de
Data de Publicação: 2020
Outros Autores: Ruiz, Jeronimo Conceição, Cardoso, Jamille Mirelle de Oliveira, Ostolin, Thais Lopes Valentim Di Paschoale, Reis, Levi Eduardo Soares, Mathias, Fernando Augusto Siqueira, Soares, Rodrigo Dian de Oliveira Aguiar, Roatt, Bruno Mendes, Oliveira, Rodrigo Corrêa de, Resende, Daniela de Melo, Reis, Alexandre Barbosa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFOP
Texto Completo: http://www.repositorio.ufop.br/jspui/handle/123456789/13647
https://doi.org/10.3390/vaccines8020252
Resumo: Many vaccine candidates against visceral leishmaniasis (VL) have been proposed; however, to date, none of them have been efficacious for the human or canine disease. On this basis, the design of leishmaniasis vaccines has been constantly changing, and the use of approaches to select specific epitopes seems to be crucial in this scenario. The ability to predict T cell-specific epitopes makes immunoinformatics an even more necessary approach, as in VL an efficient immune response against the parasite is triggered by T lymphocytes in response to Leishmania spp. immunogenic antigens. Moreover, the success of vaccines depends on the capacity to generate long-lasting memory and polyfunctional cells that are able to eliminate the parasite. In this sense, our study used a combination of different approaches to develop potential chimera candidate vaccines against VL. The first point was to identify the most immunogenic epitopes of Leishmania infantum proteins and construct chimeras composed of Major histocompatibility complex (MHC) class I and II epitopes. For this, we used immunoinformatics features. Following this, we validated these chimeras in a murine model in a thorough memory study and multifunctionality of T cells that contribute to a better elucidation of the immunological protective mechanisms of polyepitope vaccines (chimera A and B) using multicolor flow cytometry. Our results showed that in silico-designed chimeras can elicit polyfunctional T cells producing T helper (Th)1 cytokines, a strong immune response against Leishmania antigen, and the generation of central and effector memory T cells in the spleen cells of vaccinated animals that was able to reduce the parasite burden in this organ. These findings contribute two potential candidate vaccines against VL that can be used in further studies, and help in this complex field of vaccine development against this challenging parasite.
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spelling Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis.Reverse vaccinologyLeishmania infantumRational design of vaccinesMany vaccine candidates against visceral leishmaniasis (VL) have been proposed; however, to date, none of them have been efficacious for the human or canine disease. On this basis, the design of leishmaniasis vaccines has been constantly changing, and the use of approaches to select specific epitopes seems to be crucial in this scenario. The ability to predict T cell-specific epitopes makes immunoinformatics an even more necessary approach, as in VL an efficient immune response against the parasite is triggered by T lymphocytes in response to Leishmania spp. immunogenic antigens. Moreover, the success of vaccines depends on the capacity to generate long-lasting memory and polyfunctional cells that are able to eliminate the parasite. In this sense, our study used a combination of different approaches to develop potential chimera candidate vaccines against VL. The first point was to identify the most immunogenic epitopes of Leishmania infantum proteins and construct chimeras composed of Major histocompatibility complex (MHC) class I and II epitopes. For this, we used immunoinformatics features. Following this, we validated these chimeras in a murine model in a thorough memory study and multifunctionality of T cells that contribute to a better elucidation of the immunological protective mechanisms of polyepitope vaccines (chimera A and B) using multicolor flow cytometry. Our results showed that in silico-designed chimeras can elicit polyfunctional T cells producing T helper (Th)1 cytokines, a strong immune response against Leishmania antigen, and the generation of central and effector memory T cells in the spleen cells of vaccinated animals that was able to reduce the parasite burden in this organ. These findings contribute two potential candidate vaccines against VL that can be used in further studies, and help in this complex field of vaccine development against this challenging parasite.2021-09-03T15:56:05Z2021-09-03T15:56:05Z2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBRITO, R. C. F. de et al. Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis. Vaccines, v. 8, n. 2, p. 252, 2020. Disponível em: <https://www.mdpi.com/2076-393X/8/2/252>. Acesso em: 10 jun. 2021.2076-393Xhttp://www.repositorio.ufop.br/jspui/handle/123456789/13647https://doi.org/10.3390/vaccines8020252This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Fonte: o PDF do artigo.info:eu-repo/semantics/openAccessBrito, Rory Cristiane Fortes deRuiz, Jeronimo ConceiçãoCardoso, Jamille Mirelle de OliveiraOstolin, Thais Lopes Valentim Di PaschoaleReis, Levi Eduardo SoaresMathias, Fernando Augusto SiqueiraSoares, Rodrigo Dian de Oliveira AguiarRoatt, Bruno MendesOliveira, Rodrigo Corrêa deResende, Daniela de MeloReis, Alexandre Barbosaengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2021-09-03T15:56:13Zoai:repositorio.ufop.br:123456789/13647Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332021-09-03T15:56:13Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false
dc.title.none.fl_str_mv Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis.
title Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis.
spellingShingle Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis.
Brito, Rory Cristiane Fortes de
Reverse vaccinology
Leishmania infantum
Rational design of vaccines
title_short Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis.
title_full Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis.
title_fullStr Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis.
title_full_unstemmed Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis.
title_sort Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis.
author Brito, Rory Cristiane Fortes de
author_facet Brito, Rory Cristiane Fortes de
Ruiz, Jeronimo Conceição
Cardoso, Jamille Mirelle de Oliveira
Ostolin, Thais Lopes Valentim Di Paschoale
Reis, Levi Eduardo Soares
Mathias, Fernando Augusto Siqueira
Soares, Rodrigo Dian de Oliveira Aguiar
Roatt, Bruno Mendes
Oliveira, Rodrigo Corrêa de
Resende, Daniela de Melo
Reis, Alexandre Barbosa
author_role author
author2 Ruiz, Jeronimo Conceição
Cardoso, Jamille Mirelle de Oliveira
Ostolin, Thais Lopes Valentim Di Paschoale
Reis, Levi Eduardo Soares
Mathias, Fernando Augusto Siqueira
Soares, Rodrigo Dian de Oliveira Aguiar
Roatt, Bruno Mendes
Oliveira, Rodrigo Corrêa de
Resende, Daniela de Melo
Reis, Alexandre Barbosa
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Brito, Rory Cristiane Fortes de
Ruiz, Jeronimo Conceição
Cardoso, Jamille Mirelle de Oliveira
Ostolin, Thais Lopes Valentim Di Paschoale
Reis, Levi Eduardo Soares
Mathias, Fernando Augusto Siqueira
Soares, Rodrigo Dian de Oliveira Aguiar
Roatt, Bruno Mendes
Oliveira, Rodrigo Corrêa de
Resende, Daniela de Melo
Reis, Alexandre Barbosa
dc.subject.por.fl_str_mv Reverse vaccinology
Leishmania infantum
Rational design of vaccines
topic Reverse vaccinology
Leishmania infantum
Rational design of vaccines
description Many vaccine candidates against visceral leishmaniasis (VL) have been proposed; however, to date, none of them have been efficacious for the human or canine disease. On this basis, the design of leishmaniasis vaccines has been constantly changing, and the use of approaches to select specific epitopes seems to be crucial in this scenario. The ability to predict T cell-specific epitopes makes immunoinformatics an even more necessary approach, as in VL an efficient immune response against the parasite is triggered by T lymphocytes in response to Leishmania spp. immunogenic antigens. Moreover, the success of vaccines depends on the capacity to generate long-lasting memory and polyfunctional cells that are able to eliminate the parasite. In this sense, our study used a combination of different approaches to develop potential chimera candidate vaccines against VL. The first point was to identify the most immunogenic epitopes of Leishmania infantum proteins and construct chimeras composed of Major histocompatibility complex (MHC) class I and II epitopes. For this, we used immunoinformatics features. Following this, we validated these chimeras in a murine model in a thorough memory study and multifunctionality of T cells that contribute to a better elucidation of the immunological protective mechanisms of polyepitope vaccines (chimera A and B) using multicolor flow cytometry. Our results showed that in silico-designed chimeras can elicit polyfunctional T cells producing T helper (Th)1 cytokines, a strong immune response against Leishmania antigen, and the generation of central and effector memory T cells in the spleen cells of vaccinated animals that was able to reduce the parasite burden in this organ. These findings contribute two potential candidate vaccines against VL that can be used in further studies, and help in this complex field of vaccine development against this challenging parasite.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021-09-03T15:56:05Z
2021-09-03T15:56:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv BRITO, R. C. F. de et al. Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis. Vaccines, v. 8, n. 2, p. 252, 2020. Disponível em: <https://www.mdpi.com/2076-393X/8/2/252>. Acesso em: 10 jun. 2021.
2076-393X
http://www.repositorio.ufop.br/jspui/handle/123456789/13647
https://doi.org/10.3390/vaccines8020252
identifier_str_mv BRITO, R. C. F. de et al. Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis. Vaccines, v. 8, n. 2, p. 252, 2020. Disponível em: <https://www.mdpi.com/2076-393X/8/2/252>. Acesso em: 10 jun. 2021.
2076-393X
url http://www.repositorio.ufop.br/jspui/handle/123456789/13647
https://doi.org/10.3390/vaccines8020252
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFOP
instname:Universidade Federal de Ouro Preto (UFOP)
instacron:UFOP
instname_str Universidade Federal de Ouro Preto (UFOP)
instacron_str UFOP
institution UFOP
reponame_str Repositório Institucional da UFOP
collection Repositório Institucional da UFOP
repository.name.fl_str_mv Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)
repository.mail.fl_str_mv repositorio@ufop.edu.br
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