Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis.
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/jspui/handle/123456789/13647 https://doi.org/10.3390/vaccines8020252 |
Resumo: | Many vaccine candidates against visceral leishmaniasis (VL) have been proposed; however, to date, none of them have been efficacious for the human or canine disease. On this basis, the design of leishmaniasis vaccines has been constantly changing, and the use of approaches to select specific epitopes seems to be crucial in this scenario. The ability to predict T cell-specific epitopes makes immunoinformatics an even more necessary approach, as in VL an efficient immune response against the parasite is triggered by T lymphocytes in response to Leishmania spp. immunogenic antigens. Moreover, the success of vaccines depends on the capacity to generate long-lasting memory and polyfunctional cells that are able to eliminate the parasite. In this sense, our study used a combination of different approaches to develop potential chimera candidate vaccines against VL. The first point was to identify the most immunogenic epitopes of Leishmania infantum proteins and construct chimeras composed of Major histocompatibility complex (MHC) class I and II epitopes. For this, we used immunoinformatics features. Following this, we validated these chimeras in a murine model in a thorough memory study and multifunctionality of T cells that contribute to a better elucidation of the immunological protective mechanisms of polyepitope vaccines (chimera A and B) using multicolor flow cytometry. Our results showed that in silico-designed chimeras can elicit polyfunctional T cells producing T helper (Th)1 cytokines, a strong immune response against Leishmania antigen, and the generation of central and effector memory T cells in the spleen cells of vaccinated animals that was able to reduce the parasite burden in this organ. These findings contribute two potential candidate vaccines against VL that can be used in further studies, and help in this complex field of vaccine development against this challenging parasite. |
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Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis.Reverse vaccinologyLeishmania infantumRational design of vaccinesMany vaccine candidates against visceral leishmaniasis (VL) have been proposed; however, to date, none of them have been efficacious for the human or canine disease. On this basis, the design of leishmaniasis vaccines has been constantly changing, and the use of approaches to select specific epitopes seems to be crucial in this scenario. The ability to predict T cell-specific epitopes makes immunoinformatics an even more necessary approach, as in VL an efficient immune response against the parasite is triggered by T lymphocytes in response to Leishmania spp. immunogenic antigens. Moreover, the success of vaccines depends on the capacity to generate long-lasting memory and polyfunctional cells that are able to eliminate the parasite. In this sense, our study used a combination of different approaches to develop potential chimera candidate vaccines against VL. The first point was to identify the most immunogenic epitopes of Leishmania infantum proteins and construct chimeras composed of Major histocompatibility complex (MHC) class I and II epitopes. For this, we used immunoinformatics features. Following this, we validated these chimeras in a murine model in a thorough memory study and multifunctionality of T cells that contribute to a better elucidation of the immunological protective mechanisms of polyepitope vaccines (chimera A and B) using multicolor flow cytometry. Our results showed that in silico-designed chimeras can elicit polyfunctional T cells producing T helper (Th)1 cytokines, a strong immune response against Leishmania antigen, and the generation of central and effector memory T cells in the spleen cells of vaccinated animals that was able to reduce the parasite burden in this organ. These findings contribute two potential candidate vaccines against VL that can be used in further studies, and help in this complex field of vaccine development against this challenging parasite.2021-09-03T15:56:05Z2021-09-03T15:56:05Z2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBRITO, R. C. F. de et al. Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis. Vaccines, v. 8, n. 2, p. 252, 2020. Disponível em: <https://www.mdpi.com/2076-393X/8/2/252>. Acesso em: 10 jun. 2021.2076-393Xhttp://www.repositorio.ufop.br/jspui/handle/123456789/13647https://doi.org/10.3390/vaccines8020252This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Fonte: o PDF do artigo.info:eu-repo/semantics/openAccessBrito, Rory Cristiane Fortes deRuiz, Jeronimo ConceiçãoCardoso, Jamille Mirelle de OliveiraOstolin, Thais Lopes Valentim Di PaschoaleReis, Levi Eduardo SoaresMathias, Fernando Augusto SiqueiraSoares, Rodrigo Dian de Oliveira AguiarRoatt, Bruno MendesOliveira, Rodrigo Corrêa deResende, Daniela de MeloReis, Alexandre Barbosaengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2021-09-03T15:56:13Zoai:repositorio.ufop.br:123456789/13647Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332021-09-03T15:56:13Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.none.fl_str_mv |
Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis. |
title |
Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis. |
spellingShingle |
Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis. Brito, Rory Cristiane Fortes de Reverse vaccinology Leishmania infantum Rational design of vaccines |
title_short |
Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis. |
title_full |
Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis. |
title_fullStr |
Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis. |
title_full_unstemmed |
Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis. |
title_sort |
Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis. |
author |
Brito, Rory Cristiane Fortes de |
author_facet |
Brito, Rory Cristiane Fortes de Ruiz, Jeronimo Conceição Cardoso, Jamille Mirelle de Oliveira Ostolin, Thais Lopes Valentim Di Paschoale Reis, Levi Eduardo Soares Mathias, Fernando Augusto Siqueira Soares, Rodrigo Dian de Oliveira Aguiar Roatt, Bruno Mendes Oliveira, Rodrigo Corrêa de Resende, Daniela de Melo Reis, Alexandre Barbosa |
author_role |
author |
author2 |
Ruiz, Jeronimo Conceição Cardoso, Jamille Mirelle de Oliveira Ostolin, Thais Lopes Valentim Di Paschoale Reis, Levi Eduardo Soares Mathias, Fernando Augusto Siqueira Soares, Rodrigo Dian de Oliveira Aguiar Roatt, Bruno Mendes Oliveira, Rodrigo Corrêa de Resende, Daniela de Melo Reis, Alexandre Barbosa |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Brito, Rory Cristiane Fortes de Ruiz, Jeronimo Conceição Cardoso, Jamille Mirelle de Oliveira Ostolin, Thais Lopes Valentim Di Paschoale Reis, Levi Eduardo Soares Mathias, Fernando Augusto Siqueira Soares, Rodrigo Dian de Oliveira Aguiar Roatt, Bruno Mendes Oliveira, Rodrigo Corrêa de Resende, Daniela de Melo Reis, Alexandre Barbosa |
dc.subject.por.fl_str_mv |
Reverse vaccinology Leishmania infantum Rational design of vaccines |
topic |
Reverse vaccinology Leishmania infantum Rational design of vaccines |
description |
Many vaccine candidates against visceral leishmaniasis (VL) have been proposed; however, to date, none of them have been efficacious for the human or canine disease. On this basis, the design of leishmaniasis vaccines has been constantly changing, and the use of approaches to select specific epitopes seems to be crucial in this scenario. The ability to predict T cell-specific epitopes makes immunoinformatics an even more necessary approach, as in VL an efficient immune response against the parasite is triggered by T lymphocytes in response to Leishmania spp. immunogenic antigens. Moreover, the success of vaccines depends on the capacity to generate long-lasting memory and polyfunctional cells that are able to eliminate the parasite. In this sense, our study used a combination of different approaches to develop potential chimera candidate vaccines against VL. The first point was to identify the most immunogenic epitopes of Leishmania infantum proteins and construct chimeras composed of Major histocompatibility complex (MHC) class I and II epitopes. For this, we used immunoinformatics features. Following this, we validated these chimeras in a murine model in a thorough memory study and multifunctionality of T cells that contribute to a better elucidation of the immunological protective mechanisms of polyepitope vaccines (chimera A and B) using multicolor flow cytometry. Our results showed that in silico-designed chimeras can elicit polyfunctional T cells producing T helper (Th)1 cytokines, a strong immune response against Leishmania antigen, and the generation of central and effector memory T cells in the spleen cells of vaccinated animals that was able to reduce the parasite burden in this organ. These findings contribute two potential candidate vaccines against VL that can be used in further studies, and help in this complex field of vaccine development against this challenging parasite. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2021-09-03T15:56:05Z 2021-09-03T15:56:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
BRITO, R. C. F. de et al. Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis. Vaccines, v. 8, n. 2, p. 252, 2020. Disponível em: <https://www.mdpi.com/2076-393X/8/2/252>. Acesso em: 10 jun. 2021. 2076-393X http://www.repositorio.ufop.br/jspui/handle/123456789/13647 https://doi.org/10.3390/vaccines8020252 |
identifier_str_mv |
BRITO, R. C. F. de et al. Chimeric vaccines designed by immunoinformatics-activated polyfunctional and memory T cells that trigger protection against experimental visceral leishmaniasis. Vaccines, v. 8, n. 2, p. 252, 2020. Disponível em: <https://www.mdpi.com/2076-393X/8/2/252>. Acesso em: 10 jun. 2021. 2076-393X |
url |
http://www.repositorio.ufop.br/jspui/handle/123456789/13647 https://doi.org/10.3390/vaccines8020252 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFOP instname:Universidade Federal de Ouro Preto (UFOP) instacron:UFOP |
instname_str |
Universidade Federal de Ouro Preto (UFOP) |
instacron_str |
UFOP |
institution |
UFOP |
reponame_str |
Repositório Institucional da UFOP |
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Repositório Institucional da UFOP |
repository.name.fl_str_mv |
Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP) |
repository.mail.fl_str_mv |
repositorio@ufop.edu.br |
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1813002824536031232 |