Effects of the phototherapy (blue light) on the heart inflammatory response induced by Trypanosoma cruzi in mice.

Detalhes bibliográficos
Autor(a) principal: Ivanova, Natalia
Data de Publicação: 2019
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Institucional da UFOP
Texto Completo: http://www.repositorio.ufop.br/handle/123456789/11885
Resumo: Programa de Pós-Graduação em Ecologia de Biomas Tropicais. Departamento de Biodiversidade, Evolução e Meio Ambiente, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto.
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spelling Ivanova, NataliaSilva, André Talvani Pedrosa daSilva, Maria Cláudia daPerucci, Luiza OliveiraRezende, Simone AparecidaSilva, André Talvani Pedrosa daBianchi, Rodrigo Fernando2020-02-11T13:08:43Z2020-02-11T13:08:43Z2019IVANOVA, Natalia. Effects of the phototherapy (blue light) on the heart inflammatory response induced by Trypanosoma cruzi in mice. 2019. 28 f. Dissertação (Mestrado em Ecologia de Biomas Tropicais) - Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, 2019.http://www.repositorio.ufop.br/handle/123456789/11885Programa de Pós-Graduação em Ecologia de Biomas Tropicais. Departamento de Biodiversidade, Evolução e Meio Ambiente, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto.The Trypanosoma cruz, is the causative agent of Chagas disease. According to WHO (2019), currently about 7 million people are infected with T. cruzi mainly in Latin America. Despite the fact that pharmaceutical industry releases various new drugs every year, Chagas disease has only two standard substances for treatment, Benznidazole and Nifurtimox, which were recognized as chemotherapy. However, at the same time these medicines have many side effects and are inefficient in chronic phase. Nowadays the blue light is commonly used in medicine practice around the world. It was proved its antimicrobial characteristics. In the current study, were evaluated the influence of the blue light on the inflammatory parameters in C57BL6 mice (in vivo) infected with T. cruzi and as well as development of parasites in vitro. For in vitro experiment we used Y and CL strain of T. cruzi. The blue light was applied (with the pic 460 nm and 80 μW/cm2) on parasite cell culture for 5 days, 6h per day, at room temperature. The parasites were counted in 10 μL every day using the Neubauer Chamber. Treatment with the blue light led to the reduction of replication of T. cruzi in vitro. Data showed 50% reduction of parasites in experiment after 5 days of exposition, in comparison to control. For in vivo experiment was used Y strain of T. cruzi. 40 animals were separated into 4 groups: non-infected + conventional light, non-infected + blue light, infected + conventional light, and infected + blue light. Blue light was applied (with the pic 460 nm and 7 μW/cm2) for 9 days, 12 h per day. Was found that phototherapy with blue light reduce quantity of parasites circulating in the blood of animals comparing with conventional light. Blue light altered the plasma pattern of the inflammatory and regulatory mediators. After morphological analysis, was found that both infected groups exhibited parasitized cardiomyocytes with intense myocarditis with predominance of pericellular and perivascular mononuclear inflammatory infiltrate. Phototherapy with blue light reduced the quantity amastigote in the nests in cardiac tissue. These data can be used for the development of a new way of treatment for Chagas disease in the future.Autorização concedida ao Repositório Institucional da UFOP pelo(a) autor(a) em 03/02/2020 com as seguintes condições: disponível sob Licença Creative Commons 4.0 que permite copiar, distribuir e transmitir o trabalho desde que sejam citados o autor e o licenciante. Não permite o uso para fins comerciais.info:eu-repo/semantics/openAccessTrypanosoma cruziInflamaçãoFototerapiaEffects of the phototherapy (blue light) on the heart inflammatory response induced by Trypanosoma cruzi in mice.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-8924http://www.repositorio.ufop.br/bitstream/123456789/11885/5/license.txt62604f8d955274beb56c80ce1ee5dcaeMD55CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://www.repositorio.ufop.br/bitstream/123456789/11885/2/license_url924993ce0b3ba389f79f32a1b2735415MD52license_textlicense_texttext/html; charset=utf-80http://www.repositorio.ufop.br/bitstream/123456789/11885/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://www.repositorio.ufop.br/bitstream/123456789/11885/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54ORIGINALDISSERTAÇÃO_EffectsPhototherapyBlue.pdfDISSERTAÇÃO_EffectsPhototherapyBlue.pdfapplication/pdf1866316http://www.repositorio.ufop.br/bitstream/123456789/11885/1/DISSERTA%c3%87%c3%83O_EffectsPhototherapyBlue.pdfd093dccc86ad918d5f6e72e01381f4d5MD51123456789/118852020-02-11 08:08:43.902oai:localhost: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ório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332020-02-11T13:08:43Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false
dc.title.pt_BR.fl_str_mv Effects of the phototherapy (blue light) on the heart inflammatory response induced by Trypanosoma cruzi in mice.
title Effects of the phototherapy (blue light) on the heart inflammatory response induced by Trypanosoma cruzi in mice.
spellingShingle Effects of the phototherapy (blue light) on the heart inflammatory response induced by Trypanosoma cruzi in mice.
Ivanova, Natalia
Trypanosoma cruzi
Inflamação
Fototerapia
title_short Effects of the phototherapy (blue light) on the heart inflammatory response induced by Trypanosoma cruzi in mice.
title_full Effects of the phototherapy (blue light) on the heart inflammatory response induced by Trypanosoma cruzi in mice.
title_fullStr Effects of the phototherapy (blue light) on the heart inflammatory response induced by Trypanosoma cruzi in mice.
title_full_unstemmed Effects of the phototherapy (blue light) on the heart inflammatory response induced by Trypanosoma cruzi in mice.
title_sort Effects of the phototherapy (blue light) on the heart inflammatory response induced by Trypanosoma cruzi in mice.
author Ivanova, Natalia
author_facet Ivanova, Natalia
author_role author
dc.contributor.referee.pt_BR.fl_str_mv Silva, André Talvani Pedrosa da
Silva, Maria Cláudia da
Perucci, Luiza Oliveira
Rezende, Simone Aparecida
dc.contributor.author.fl_str_mv Ivanova, Natalia
dc.contributor.advisor1.fl_str_mv Silva, André Talvani Pedrosa da
Bianchi, Rodrigo Fernando
contributor_str_mv Silva, André Talvani Pedrosa da
Bianchi, Rodrigo Fernando
dc.subject.por.fl_str_mv Trypanosoma cruzi
Inflamação
Fototerapia
topic Trypanosoma cruzi
Inflamação
Fototerapia
description Programa de Pós-Graduação em Ecologia de Biomas Tropicais. Departamento de Biodiversidade, Evolução e Meio Ambiente, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto.
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2020-02-11T13:08:43Z
dc.date.available.fl_str_mv 2020-02-11T13:08:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
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dc.identifier.citation.fl_str_mv IVANOVA, Natalia. Effects of the phototherapy (blue light) on the heart inflammatory response induced by Trypanosoma cruzi in mice. 2019. 28 f. Dissertação (Mestrado em Ecologia de Biomas Tropicais) - Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, 2019.
dc.identifier.uri.fl_str_mv http://www.repositorio.ufop.br/handle/123456789/11885
identifier_str_mv IVANOVA, Natalia. Effects of the phototherapy (blue light) on the heart inflammatory response induced by Trypanosoma cruzi in mice. 2019. 28 f. Dissertação (Mestrado em Ecologia de Biomas Tropicais) - Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, 2019.
url http://www.repositorio.ufop.br/handle/123456789/11885
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