Chemical analysis of Eruca sativa ethanolic extract and its effects on hyperuricaemia.

• Autor(a) principal: Teixeira, Arthur Ferrari
• Data de Publicação: 2022
• Outros Autores: Souza, Jacqueline de, Dophine, Douglas Daniel, Souza Filho, José Dias de, Guimarães, Dênia Antunes Saúde
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFOP
• dARK ID: ark:/61566/001300000j547
• Texto Completo: http://www.repositorio.ufop.br/jspui/handle/123456789/15750; https://doi.org/10.3390/molecules27051506
• topic: Glucosinolate; Glucosylated flavonols; Rocket; Validation; NMR

In vivo assays and chemical analyses were performed on the ethanolic extract from leaves of Eruca sativa. UHPLC-ESI-QTOF analysis confirmed the presence of glucosinolates and flavonol glucosides. The major flavonoid of the ethanolic extract, kaempferol-3,40 -di-O-β-glucoside, was isolated, a HPLC-DAD method developed and validated to quantify its content in the extract. In vivo experiments were carried out on Wistar rats with hyperuricaemia induced by potassium oxonate and uric acid. A hypouricaemic effect was observed in hyperuricaemic Wistar rats treated with ethanolic extract at dose of 125 mg/kg and kaempferol-3,40 -di-O-β-glucoside at dose of 10 mg/kg. The main anti-hyperuricaemic mechanism observed in the extract was uricosuric. Kaempferol-3,40 -di-O-β- glucoside was identified as an important component responsible for the total activity of the ethanolic extract and was considered as a good chemical and biological marker of the ethanolic extract of E. sativa. The obtained results indicated the potential of E. sativa in the treatment of hyperuricaemia and its comorbidities.