Experimental steering of electron microscopy studies using prior X-ray computed tomography.

• Autor(a) principal: Starborg, Tobias
• Data de Publicação: 2019
• Outros Autores: O'Sullivan, James, Carneiro, Cláudia Martins, Behnsen, Julia, Else, Kathryn, Grencis, Richard, Withers, Philip John
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFOP
• Texto Completo: http://www.repositorio.ufop.br/handle/123456789/12175; https://doi.org/10.1016/j.ultramic.2019.03.002
• topic: Ultramicrotomy; Multiscale imaging; Pre-screening; Scanning electron microscopy

Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) can provide unrivalled high-resolution images of specific features and volumes of interest. However, the regions interrogated are typically very small, and sample preparation is both time-consuming and destructive. Here we consider how prior X-ray micro-computed tomography (microCT) presents an opportunity to increase the efficiency of electron microscopy in biology. We demonstrate how it can be used to; select the most promising samples and target site-specific locations; provide a wider context of the location being interrogated (multiscale correlative imaging); guide sample preparation and 3D imaging schemes; as well as quantify the effects of destructive sample preparation and staining procedures. We present a workflow utilising open source software in which microCT can be used either broadly, or precisely, to experimentally steer and inform subsequent electron microscopy studies. As automated sample registration procedures are developed to enable correlative microscopy, experimental steering by prior CT could be beneficially routinely incorporated into many experimental workflows.