Parasite control and skeletal myositis in Trypanosoma cruzi-infected and exercised rats.
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/9159 https://doi.org/10.1016/j.actatropica.2017.02.012 |
Resumo: | Non-pharmacological strategies have been rarely described in the treatment of infectious diseases.Although exercise training has been recently incorporated in the clinical management of Chagas disease,the rationale basis that supports this indication is poorly understood. Thus, we investigated the effectof an aerobic exercise on the parasitism, inflammation and oxidative tissue damage in a murine modelof Trypanosoma cruzi-induced skeletal myositis. Wistar rats were randomized into four groups: trainednot infected (TNI) and infected (TI), sedentary not infected (SNI) and infected (SI). A running trainingprogram was administered 5 days/week for 9 weeks. Then, infected animals were inoculated with T. cruziand followed up for another 9 weeks. Exercise training induced beneficial adaptations by increasing timeto fatigue and lactate threshold in TNI and TI animals. SI animals presented higher parasitemia, skele-tal muscle parasitism, cell necrosis, leukocyte infiltration, cytokines levels, reactive oxygen species andnitric oxide production, thiobarbituric acid reactive substances, carbonyl proteins, myosin heavy chain Idepletion, and increased catalase (CAT) and superoxide dismutase (SOD) activities. Beyond attenuationin all these variables, TI animals showed reduced TNF- , CCL-2/MCP-1 and CX3CL1, and increased IL-10muscle levels. Furthermore, these animals presented higher CAT and SOD activities and reduced lipidand protein oxidation. Taken together, our findings indicated that exercise training induced a protectivephenotype in T. cruzi-infected mice, enhancing host defenses against the parasite and attenuating thepathological remodeling associated with skeletal myositis, aspects potentially associated to an improvedimmunological and redox balance in infected animals. |
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Novaes, Rômulo DiasGonçalves, Reggiani VilelaPenitente, Arlete RitaCupertino, Marli do CarmoMaldonado, Izabel Regina dos Santos CostaSilva, André Talvani Pedrosa daNatali, Antônio José2017-11-17T13:34:05Z2017-11-17T13:34:05Z2017NOVAES, R. D. et al. Parasite control and skeletal myositis in Trypanosoma cruzi-infected and exercised rats. Acta Tropica, v. 170, p. 8-15, 2017. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0001706X16310567?via%3Dihub>. Acesso em: 15 set. 2017.0001-706Xhttp://www.repositorio.ufop.br/handle/123456789/9159https://doi.org/10.1016/j.actatropica.2017.02.012Non-pharmacological strategies have been rarely described in the treatment of infectious diseases.Although exercise training has been recently incorporated in the clinical management of Chagas disease,the rationale basis that supports this indication is poorly understood. Thus, we investigated the effectof an aerobic exercise on the parasitism, inflammation and oxidative tissue damage in a murine modelof Trypanosoma cruzi-induced skeletal myositis. Wistar rats were randomized into four groups: trainednot infected (TNI) and infected (TI), sedentary not infected (SNI) and infected (SI). A running trainingprogram was administered 5 days/week for 9 weeks. Then, infected animals were inoculated with T. cruziand followed up for another 9 weeks. Exercise training induced beneficial adaptations by increasing timeto fatigue and lactate threshold in TNI and TI animals. SI animals presented higher parasitemia, skele-tal muscle parasitism, cell necrosis, leukocyte infiltration, cytokines levels, reactive oxygen species andnitric oxide production, thiobarbituric acid reactive substances, carbonyl proteins, myosin heavy chain Idepletion, and increased catalase (CAT) and superoxide dismutase (SOD) activities. Beyond attenuationin all these variables, TI animals showed reduced TNF- , CCL-2/MCP-1 and CX3CL1, and increased IL-10muscle levels. Furthermore, these animals presented higher CAT and SOD activities and reduced lipidand protein oxidation. Taken together, our findings indicated that exercise training induced a protectivephenotype in T. cruzi-infected mice, enhancing host defenses against the parasite and attenuating thepathological remodeling associated with skeletal myositis, aspects potentially associated to an improvedimmunological and redox balance in infected animals.O periódico Acta Tropica concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 4210820872260.info:eu-repo/semantics/openAccessExercise trainingOxidative stressSkeletal muscleParasite control and skeletal myositis in Trypanosoma cruzi-infected and exercised rats.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-8924http://www.repositorio.ufop.br/bitstream/123456789/9159/2/license.txt62604f8d955274beb56c80ce1ee5dcaeMD52ORIGINALARTIGO_ParasiteControlSkeletal.pdfARTIGO_ParasiteControlSkeletal.pdfapplication/pdf1619041http://www.repositorio.ufop.br/bitstream/123456789/9159/1/ARTIGO_ParasiteControlSkeletal.pdfe1f04d14afa981c8cb1f2cd7631b1314MD51123456789/91592020-02-20 05:44:45.783oai:localhost: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ório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332020-02-20T10:44:45Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Parasite control and skeletal myositis in Trypanosoma cruzi-infected and exercised rats. |
title |
Parasite control and skeletal myositis in Trypanosoma cruzi-infected and exercised rats. |
spellingShingle |
Parasite control and skeletal myositis in Trypanosoma cruzi-infected and exercised rats. Novaes, Rômulo Dias Exercise training Oxidative stress Skeletal muscle |
title_short |
Parasite control and skeletal myositis in Trypanosoma cruzi-infected and exercised rats. |
title_full |
Parasite control and skeletal myositis in Trypanosoma cruzi-infected and exercised rats. |
title_fullStr |
Parasite control and skeletal myositis in Trypanosoma cruzi-infected and exercised rats. |
title_full_unstemmed |
Parasite control and skeletal myositis in Trypanosoma cruzi-infected and exercised rats. |
title_sort |
Parasite control and skeletal myositis in Trypanosoma cruzi-infected and exercised rats. |
author |
Novaes, Rômulo Dias |
author_facet |
Novaes, Rômulo Dias Gonçalves, Reggiani Vilela Penitente, Arlete Rita Cupertino, Marli do Carmo Maldonado, Izabel Regina dos Santos Costa Silva, André Talvani Pedrosa da Natali, Antônio José |
author_role |
author |
author2 |
Gonçalves, Reggiani Vilela Penitente, Arlete Rita Cupertino, Marli do Carmo Maldonado, Izabel Regina dos Santos Costa Silva, André Talvani Pedrosa da Natali, Antônio José |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Novaes, Rômulo Dias Gonçalves, Reggiani Vilela Penitente, Arlete Rita Cupertino, Marli do Carmo Maldonado, Izabel Regina dos Santos Costa Silva, André Talvani Pedrosa da Natali, Antônio José |
dc.subject.por.fl_str_mv |
Exercise training Oxidative stress Skeletal muscle |
topic |
Exercise training Oxidative stress Skeletal muscle |
description |
Non-pharmacological strategies have been rarely described in the treatment of infectious diseases.Although exercise training has been recently incorporated in the clinical management of Chagas disease,the rationale basis that supports this indication is poorly understood. Thus, we investigated the effectof an aerobic exercise on the parasitism, inflammation and oxidative tissue damage in a murine modelof Trypanosoma cruzi-induced skeletal myositis. Wistar rats were randomized into four groups: trainednot infected (TNI) and infected (TI), sedentary not infected (SNI) and infected (SI). A running trainingprogram was administered 5 days/week for 9 weeks. Then, infected animals were inoculated with T. cruziand followed up for another 9 weeks. Exercise training induced beneficial adaptations by increasing timeto fatigue and lactate threshold in TNI and TI animals. SI animals presented higher parasitemia, skele-tal muscle parasitism, cell necrosis, leukocyte infiltration, cytokines levels, reactive oxygen species andnitric oxide production, thiobarbituric acid reactive substances, carbonyl proteins, myosin heavy chain Idepletion, and increased catalase (CAT) and superoxide dismutase (SOD) activities. Beyond attenuationin all these variables, TI animals showed reduced TNF- , CCL-2/MCP-1 and CX3CL1, and increased IL-10muscle levels. Furthermore, these animals presented higher CAT and SOD activities and reduced lipidand protein oxidation. Taken together, our findings indicated that exercise training induced a protectivephenotype in T. cruzi-infected mice, enhancing host defenses against the parasite and attenuating thepathological remodeling associated with skeletal myositis, aspects potentially associated to an improvedimmunological and redox balance in infected animals. |
publishDate |
2017 |
dc.date.accessioned.fl_str_mv |
2017-11-17T13:34:05Z |
dc.date.available.fl_str_mv |
2017-11-17T13:34:05Z |
dc.date.issued.fl_str_mv |
2017 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
NOVAES, R. D. et al. Parasite control and skeletal myositis in Trypanosoma cruzi-infected and exercised rats. Acta Tropica, v. 170, p. 8-15, 2017. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0001706X16310567?via%3Dihub>. Acesso em: 15 set. 2017. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/9159 |
dc.identifier.issn.none.fl_str_mv |
0001-706X |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.actatropica.2017.02.012 |
identifier_str_mv |
NOVAES, R. D. et al. Parasite control and skeletal myositis in Trypanosoma cruzi-infected and exercised rats. Acta Tropica, v. 170, p. 8-15, 2017. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0001706X16310567?via%3Dihub>. Acesso em: 15 set. 2017. 0001-706X |
url |
http://www.repositorio.ufop.br/handle/123456789/9159 https://doi.org/10.1016/j.actatropica.2017.02.012 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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reponame:Repositório Institucional da UFOP instname:Universidade Federal de Ouro Preto (UFOP) instacron:UFOP |
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