Trypanosoma cruzi I and IV stocks from Brazilian Amazon are divergent in terms of biological and medical properties in mice.

Detalhes bibliográficos
Autor(a) principal: Monteiro, Wuelton Marcelo
Data de Publicação: 2013
Outros Autores: Teston, Ana Paula Margioto, Gruendling, Ana Paula, Reis, Daniele dos, Gomes, Mônica Lúcia, Araújo, Silvana Marques de, Bahia, Maria Terezinha, Magalhães, Laylah Kelre Costa, Guerra, Jorge Augusto de Oliveira, Silveira, Henrique, Toledo, Max Jean de Ornelas, Barbosa, Maria das Graças Vale
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFOP
Texto Completo: http://www.repositorio.ufop.br/handle/123456789/4926
https://doi.org/10.1371/journal.pntd.0002069
Resumo: Background: In the Brazilian Amazon, clinical and epidemiological frameworks of Chagas disease are very dissimilar in relation to the endemic classical areas of transmission, possibly due to genetic and biological characteristics of the circulating Trypanosoma cruzi stocks. Twenty six T. cruzi stocks from Western Amazon Region attributed to the TcI and TcIV DTUs were comparatively studied in Swiss mice to test the hypothesis that T. cruzi clonal structure has a major impact on its biological and medical properties. Methodology/Principal Findings: Seventeen parameters were assayed in mice infected with 14 T. cruzi strains belonging to DTU TcI and 11 strains typed as TcIV. In comparison with TcI, TcIV stocks promoted a significantly shorter pre-patent period (p,0.001), a longer patent period (p,0.001), higher values of mean daily parasitemia (p = 0.009) and maximum of parasitemia (p = 0.015), earlier days of maximum parasitemia (p,0.001) and mortality (p = 0.018), higher mortality rates in the acute phase (p = 0.047), higher infectivity rates (p = 0.002), higher positivity in the fresh blood examination (p,0.001), higher positivity in the ELISA at the early chronic phase (p = 0.022), and a higher positivity in the ELISA at the late chronic phase (p = 0.003). On the other hand TcI showed higher values of mortality rates in the early chronic phase (p = 0.014), higher frequency of mice with inflammatory process in any organ (p = 0.005), higher frequency of mice with tissue parasitism in any organ (p = 0.027) and a higher susceptibility to benznidazole (p = 0.002) than TcIV. Survival analysis showing the time elapsed from the day of inoculation to the beginning of the patent period was significantly shorter for TcIV strains and the death episodes triggered following the infection with TcI occurred significantly later in relation to TcIV. The notable exceptions come from positivity in the hemocultures and PCR, for which the results were similar. Conclusion/Significance: T. cruzi stocks belonging to TcI and TcIV DTUs from Brazilian Amazon are divergent in terms of biological and medical properties in mice.
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spelling Trypanosoma cruzi I and IV stocks from Brazilian Amazon are divergent in terms of biological and medical properties in mice.Background: In the Brazilian Amazon, clinical and epidemiological frameworks of Chagas disease are very dissimilar in relation to the endemic classical areas of transmission, possibly due to genetic and biological characteristics of the circulating Trypanosoma cruzi stocks. Twenty six T. cruzi stocks from Western Amazon Region attributed to the TcI and TcIV DTUs were comparatively studied in Swiss mice to test the hypothesis that T. cruzi clonal structure has a major impact on its biological and medical properties. Methodology/Principal Findings: Seventeen parameters were assayed in mice infected with 14 T. cruzi strains belonging to DTU TcI and 11 strains typed as TcIV. In comparison with TcI, TcIV stocks promoted a significantly shorter pre-patent period (p,0.001), a longer patent period (p,0.001), higher values of mean daily parasitemia (p = 0.009) and maximum of parasitemia (p = 0.015), earlier days of maximum parasitemia (p,0.001) and mortality (p = 0.018), higher mortality rates in the acute phase (p = 0.047), higher infectivity rates (p = 0.002), higher positivity in the fresh blood examination (p,0.001), higher positivity in the ELISA at the early chronic phase (p = 0.022), and a higher positivity in the ELISA at the late chronic phase (p = 0.003). On the other hand TcI showed higher values of mortality rates in the early chronic phase (p = 0.014), higher frequency of mice with inflammatory process in any organ (p = 0.005), higher frequency of mice with tissue parasitism in any organ (p = 0.027) and a higher susceptibility to benznidazole (p = 0.002) than TcIV. Survival analysis showing the time elapsed from the day of inoculation to the beginning of the patent period was significantly shorter for TcIV strains and the death episodes triggered following the infection with TcI occurred significantly later in relation to TcIV. The notable exceptions come from positivity in the hemocultures and PCR, for which the results were similar. Conclusion/Significance: T. cruzi stocks belonging to TcI and TcIV DTUs from Brazilian Amazon are divergent in terms of biological and medical properties in mice.2015-04-01T17:34:19Z2015-04-01T17:34:19Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMONTEIRO, W. M. et al. Trypanosoma cruzi I and IV stocks from Brazilian Amazon are divergent in terms of biological and medical properties in mice. PLoS Neglected Tropical Diseases, v. 7, p. e2069, 2013. Disponível em: <http://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0002069>. Acesso em: 08 nov. 2014.1932-6203http://www.repositorio.ufop.br/handle/123456789/4926https://doi.org/10.1371/journal.pntd.0002069Os trabalhos publicados na Plos one estão sob Licença Creative Commons que permite copiar, distribuir e transmitir o trabalho, desde que sejam citados o autor e licenciante. Não permite o uso para fins comerciais nem a adaptação. Fonte: Plos one <https://www.plos.org/open-access>. Acesso em: 03 jan. 2017.info:eu-repo/semantics/openAccessMonteiro, Wuelton MarceloTeston, Ana Paula MargiotoGruendling, Ana PaulaReis, Daniele dosGomes, Mônica LúciaAraújo, Silvana Marques deBahia, Maria TerezinhaMagalhães, Laylah Kelre CostaGuerra, Jorge Augusto de OliveiraSilveira, HenriqueToledo, Max Jean de OrnelasBarbosa, Maria das Graças Valeengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2019-07-04T17:29:13Zoai:repositorio.ufop.br:123456789/4926Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-07-04T17:29:13Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false
dc.title.none.fl_str_mv Trypanosoma cruzi I and IV stocks from Brazilian Amazon are divergent in terms of biological and medical properties in mice.
title Trypanosoma cruzi I and IV stocks from Brazilian Amazon are divergent in terms of biological and medical properties in mice.
spellingShingle Trypanosoma cruzi I and IV stocks from Brazilian Amazon are divergent in terms of biological and medical properties in mice.
Monteiro, Wuelton Marcelo
title_short Trypanosoma cruzi I and IV stocks from Brazilian Amazon are divergent in terms of biological and medical properties in mice.
title_full Trypanosoma cruzi I and IV stocks from Brazilian Amazon are divergent in terms of biological and medical properties in mice.
title_fullStr Trypanosoma cruzi I and IV stocks from Brazilian Amazon are divergent in terms of biological and medical properties in mice.
title_full_unstemmed Trypanosoma cruzi I and IV stocks from Brazilian Amazon are divergent in terms of biological and medical properties in mice.
title_sort Trypanosoma cruzi I and IV stocks from Brazilian Amazon are divergent in terms of biological and medical properties in mice.
author Monteiro, Wuelton Marcelo
author_facet Monteiro, Wuelton Marcelo
Teston, Ana Paula Margioto
Gruendling, Ana Paula
Reis, Daniele dos
Gomes, Mônica Lúcia
Araújo, Silvana Marques de
Bahia, Maria Terezinha
Magalhães, Laylah Kelre Costa
Guerra, Jorge Augusto de Oliveira
Silveira, Henrique
Toledo, Max Jean de Ornelas
Barbosa, Maria das Graças Vale
author_role author
author2 Teston, Ana Paula Margioto
Gruendling, Ana Paula
Reis, Daniele dos
Gomes, Mônica Lúcia
Araújo, Silvana Marques de
Bahia, Maria Terezinha
Magalhães, Laylah Kelre Costa
Guerra, Jorge Augusto de Oliveira
Silveira, Henrique
Toledo, Max Jean de Ornelas
Barbosa, Maria das Graças Vale
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Monteiro, Wuelton Marcelo
Teston, Ana Paula Margioto
Gruendling, Ana Paula
Reis, Daniele dos
Gomes, Mônica Lúcia
Araújo, Silvana Marques de
Bahia, Maria Terezinha
Magalhães, Laylah Kelre Costa
Guerra, Jorge Augusto de Oliveira
Silveira, Henrique
Toledo, Max Jean de Ornelas
Barbosa, Maria das Graças Vale
description Background: In the Brazilian Amazon, clinical and epidemiological frameworks of Chagas disease are very dissimilar in relation to the endemic classical areas of transmission, possibly due to genetic and biological characteristics of the circulating Trypanosoma cruzi stocks. Twenty six T. cruzi stocks from Western Amazon Region attributed to the TcI and TcIV DTUs were comparatively studied in Swiss mice to test the hypothesis that T. cruzi clonal structure has a major impact on its biological and medical properties. Methodology/Principal Findings: Seventeen parameters were assayed in mice infected with 14 T. cruzi strains belonging to DTU TcI and 11 strains typed as TcIV. In comparison with TcI, TcIV stocks promoted a significantly shorter pre-patent period (p,0.001), a longer patent period (p,0.001), higher values of mean daily parasitemia (p = 0.009) and maximum of parasitemia (p = 0.015), earlier days of maximum parasitemia (p,0.001) and mortality (p = 0.018), higher mortality rates in the acute phase (p = 0.047), higher infectivity rates (p = 0.002), higher positivity in the fresh blood examination (p,0.001), higher positivity in the ELISA at the early chronic phase (p = 0.022), and a higher positivity in the ELISA at the late chronic phase (p = 0.003). On the other hand TcI showed higher values of mortality rates in the early chronic phase (p = 0.014), higher frequency of mice with inflammatory process in any organ (p = 0.005), higher frequency of mice with tissue parasitism in any organ (p = 0.027) and a higher susceptibility to benznidazole (p = 0.002) than TcIV. Survival analysis showing the time elapsed from the day of inoculation to the beginning of the patent period was significantly shorter for TcIV strains and the death episodes triggered following the infection with TcI occurred significantly later in relation to TcIV. The notable exceptions come from positivity in the hemocultures and PCR, for which the results were similar. Conclusion/Significance: T. cruzi stocks belonging to TcI and TcIV DTUs from Brazilian Amazon are divergent in terms of biological and medical properties in mice.
publishDate 2013
dc.date.none.fl_str_mv 2013
2015-04-01T17:34:19Z
2015-04-01T17:34:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv MONTEIRO, W. M. et al. Trypanosoma cruzi I and IV stocks from Brazilian Amazon are divergent in terms of biological and medical properties in mice. PLoS Neglected Tropical Diseases, v. 7, p. e2069, 2013. Disponível em: <http://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0002069>. Acesso em: 08 nov. 2014.
1932-6203
http://www.repositorio.ufop.br/handle/123456789/4926
https://doi.org/10.1371/journal.pntd.0002069
identifier_str_mv MONTEIRO, W. M. et al. Trypanosoma cruzi I and IV stocks from Brazilian Amazon are divergent in terms of biological and medical properties in mice. PLoS Neglected Tropical Diseases, v. 7, p. e2069, 2013. Disponível em: <http://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0002069>. Acesso em: 08 nov. 2014.
1932-6203
url http://www.repositorio.ufop.br/handle/123456789/4926
https://doi.org/10.1371/journal.pntd.0002069
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