Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effecting on calcium channels.

Detalhes bibliográficos
Autor(a) principal: Dutra, Alexandre Augusto de Assis
Data de Publicação: 2008
Outros Autores: Sousa, Lucas Oliveira, Resende, Rodrigo Ribeiro, Brandão, Rogélio Lopes, Kalapothakis, Evanguedes, Castro, Ieso de Miranda
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFOP
Texto Completo: http://www.repositorio.ufop.br/handle/123456789/1165
Resumo: Here, we described the expression and characterization of the recombinant toxin LTx2, which was previously isolated from the venomous cDNA library of a Brazilian spider, Lasiodora sp. (Mygalomorphae, Theraphosidae). The recombinant toxin found in the soluble and insoluble fractions was purified by reverse phase high-performance liquid chromatography (HPLC). Ca2+ imaging analysis revealed that the recombinant LTx2 acts on calcium channels of BC3H1 cells, blocking L-type calcium channels.
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spelling Dutra, Alexandre Augusto de AssisSousa, Lucas OliveiraResende, Rodrigo RibeiroBrandão, Rogélio LopesKalapothakis, EvanguedesCastro, Ieso de Miranda2012-07-18T18:10:30Z2012-07-18T18:10:30Z2008DUTRA, A. A. A. et al. Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effecting on calcium channels. Peptides, v. 29, n. 9, p. 1505-1513, set. 2008. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0196978108002131. Acesso em: 18 jul. 2012.01969781http://www.repositorio.ufop.br/handle/123456789/1165Here, we described the expression and characterization of the recombinant toxin LTx2, which was previously isolated from the venomous cDNA library of a Brazilian spider, Lasiodora sp. (Mygalomorphae, Theraphosidae). The recombinant toxin found in the soluble and insoluble fractions was purified by reverse phase high-performance liquid chromatography (HPLC). Ca2+ imaging analysis revealed that the recombinant LTx2 acts on calcium channels of BC3H1 cells, blocking L-type calcium channels.Calcium channelVenomSpider toxinsLasiodoracDNAExpressionExpression and characterization of LTx2, a neurotoxin from Lasiodora sp. effecting on calcium channels.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleO periódico Peptides concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3266011353103.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://www.repositorio.ufop.br/bitstream/123456789/1165/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD55ORIGINALARTIGO_ExpressionCharacterizationNeurotoxin.pdfARTIGO_ExpressionCharacterizationNeurotoxin.pdfapplication/pdf920362http://www.repositorio.ufop.br/bitstream/123456789/1165/1/ARTIGO_ExpressionCharacterizationNeurotoxin.pdf959eeeeb74de57e152f825b94d2295f7MD51123456789/11652019-02-28 08:51:33.349oai:localhost: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Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-02-28T13:51:33Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false
dc.title.pt_BR.fl_str_mv Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effecting on calcium channels.
title Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effecting on calcium channels.
spellingShingle Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effecting on calcium channels.
Dutra, Alexandre Augusto de Assis
Calcium channel
Venom
Spider toxins
Lasiodora
cDNA
Expression
title_short Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effecting on calcium channels.
title_full Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effecting on calcium channels.
title_fullStr Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effecting on calcium channels.
title_full_unstemmed Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effecting on calcium channels.
title_sort Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effecting on calcium channels.
author Dutra, Alexandre Augusto de Assis
author_facet Dutra, Alexandre Augusto de Assis
Sousa, Lucas Oliveira
Resende, Rodrigo Ribeiro
Brandão, Rogélio Lopes
Kalapothakis, Evanguedes
Castro, Ieso de Miranda
author_role author
author2 Sousa, Lucas Oliveira
Resende, Rodrigo Ribeiro
Brandão, Rogélio Lopes
Kalapothakis, Evanguedes
Castro, Ieso de Miranda
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Dutra, Alexandre Augusto de Assis
Sousa, Lucas Oliveira
Resende, Rodrigo Ribeiro
Brandão, Rogélio Lopes
Kalapothakis, Evanguedes
Castro, Ieso de Miranda
dc.subject.por.fl_str_mv Calcium channel
Venom
Spider toxins
Lasiodora
cDNA
Expression
topic Calcium channel
Venom
Spider toxins
Lasiodora
cDNA
Expression
description Here, we described the expression and characterization of the recombinant toxin LTx2, which was previously isolated from the venomous cDNA library of a Brazilian spider, Lasiodora sp. (Mygalomorphae, Theraphosidae). The recombinant toxin found in the soluble and insoluble fractions was purified by reverse phase high-performance liquid chromatography (HPLC). Ca2+ imaging analysis revealed that the recombinant LTx2 acts on calcium channels of BC3H1 cells, blocking L-type calcium channels.
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2012-07-18T18:10:30Z
dc.date.available.fl_str_mv 2012-07-18T18:10:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv DUTRA, A. A. A. et al. Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effecting on calcium channels. Peptides, v. 29, n. 9, p. 1505-1513, set. 2008. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0196978108002131. Acesso em: 18 jul. 2012.
dc.identifier.uri.fl_str_mv http://www.repositorio.ufop.br/handle/123456789/1165
dc.identifier.issn.none.fl_str_mv 01969781
identifier_str_mv DUTRA, A. A. A. et al. Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effecting on calcium channels. Peptides, v. 29, n. 9, p. 1505-1513, set. 2008. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0196978108002131. Acesso em: 18 jul. 2012.
01969781
url http://www.repositorio.ufop.br/handle/123456789/1165
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFOP
instname:Universidade Federal de Ouro Preto (UFOP)
instacron:UFOP
instname_str Universidade Federal de Ouro Preto (UFOP)
instacron_str UFOP
institution UFOP
reponame_str Repositório Institucional da UFOP
collection Repositório Institucional da UFOP
bitstream.url.fl_str_mv http://www.repositorio.ufop.br/bitstream/123456789/1165/5/license.txt
http://www.repositorio.ufop.br/bitstream/123456789/1165/1/ARTIGO_ExpressionCharacterizationNeurotoxin.pdf
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