Carbonyl cyanidem - chlorophenylhydrazone induced calcium signaling and activation of plasma membrane H1-ATPase in the yeast Saccharomyces cerevisiae.

• Autor(a) principal: Pereira, Michele B. P.
• Data de Publicação: 2008
• Outros Autores: Tisi, Renata, Fietto, Luciano Gomes, Cardoso, Anamaria de Souza, França, Mônica M., Carvalho, Fernanda Machado de, Trópia, Maria José Magalhães, Martegani, Enzo, Castro, Ieso de Miranda, Brandão, Rogélio Lopes
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFOP
• Texto Completo: http://www.repositorio.ufop.br/handle/123456789/1164
• topic: Depolarizing compounds; Saccharomyces cerevisiae

The plasma membrane H1-ATPase from Saccharomyces cerevisiae is an enzyme that plays a very important role in the yeast physiology. The addition of protonophores, such as 2,4-dinitrophenol (DNP) and carbonyl cyanide m-chlorophenylhydrazone (CCCP), also triggers a clear in vivo activation of this enzyme. Here, we demonstrate that CCCP-induced activation of the plasma membrane H1-ATPase shares some similarities with the sugar-induced activation of the enzyme. Phospholipase C and protein kinase C activities are essential for this activation process while Gpa2p, a G protein involved in the glucose-induced activation of the ATPase, is not required. CCCP also induces a phospholipase Cdependent increase in intracellular calcium. Moreover, we show that the availability of extracellular calcium is required for CCCP stimulation of H1-ATPase, suggesting a possible connection between calcium signaling and activation of ATPase.