Serratia liquefaciens FG3 isolated from a metallophyte plant sheds light on the evolution and mechanisms of adaptive traits in extreme environments.

• Autor(a) principal: Caneschi, Washington Luiz
• Data de Publicação: 2019
• Outros Autores: Sanchez, Angelica Bianchini, Felestrino, Érica Barbosa, Lemes, Camila Gracyelle de Carvalho, Cordeiro, Isabella Ferreira, Fonseca, Natasha Peixoto, Villa, Morghana Marina, Vieira, Izadora Tabuso, Moraes, Lauro Ângelo Gonçalves de, Assis, Renata de Almeida Barbosa, Carmo, Flávio Fonseca do, Kamino, Luciana Hiromi Yoshino, Silva, Robson Soares, Ferro, Jesus Aparecido, Ferro, Maria Inês Tiraboschi, Ferreira, Rafael Marini, Santos, Vera Lúcia, Silva, Ubiana de Cássia Mourão, Almeida Junior, Nalvo Franco de, Varani, Alessandro de Mello, Garcia, Camila Carrião Machado, Setubal, João Carlos, Moreira, Leandro Marcio
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFOP
• Texto Completo: http://www.repositorio.ufop.br/handle/123456789/12006; https://doi.org/10.1038/s41598-019-54601-4

Serratia liquefaciens strain FG3 (SlFG3), isolated from the flower of Stachytarpheta glabra in the Brazilian ferruginous fields, has distinctive genomic, adaptive, and biotechnological potential. Herein, using a combination of genomics and molecular approaches, we unlocked the evolution of the adaptive traits acquired by S1FG3, which exhibits the second largest chromosome containing the largest conjugative plasmids described for Serratia. Comparative analysis revealed the presence of 18 genomic islands and 311 unique protein families involved in distinct adaptive features. S1FG3 has a diversified repertoire of genes associated with Nonribosomal peptides (NRPs/PKS), a complete and functional cluster related to cellulose synthesis, and an extensive and functional repertoire of oxidative metabolism genes. In addition, S1FG3 possesses a complete pathway related to protocatecuate and chloroaromatic degradation, and a complete repertoire of genes related to DNA repair and protection that includes mechanisms related to UV light tolerance, redox process resistance, and a laterally acquired capacity to protect DNA using phosphorothioation. These findings summarize that SlFG3 is well-adapted to different biotic and abiotic stress situations imposed by extreme conditions associated with ferruginous fields, unlocking the impact of the lateral gene transfer to adjust the genome for extreme environments, and providing insight into the evolution of prokaryotes.