Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system.
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/9231 https://doi.org/10.1016/j.molbiopara.2017.01.005 |
Resumo: | tIn recent years, proteasome involvement in the damage response induced by ionizing radiation (IR)became evident. However, whether proteasome plays a direct or indirect role in IR-induced damageresponse still unclear. Trypanosoma cruzi is a human parasite capable of remarkable high tolerance toIR, suggesting a highly efficient damage response system. Here, we investigate the role of T. cruzi pro-teasome in the damage response induced by IR. We exposed epimastigotes to high doses of gamma rayand we analyzed the expression and subcellular localization of several components of the ubiquitin-proteasome system. We show that proteasome inhibition increases IR-induced cell growth arrest andproteasome-mediated proteolysis is altered after parasite exposure. We observed nuclear accumulationof 19S and 20S proteasome subunits in response to IR treatments. Intriguingly, the dynamic of 19S par-ticle nuclear accumulation was more similar to the dynamic observed for Rad51 nuclear translocationthan the observed for 20S. In the other hand, 20S increase and nuclear translocation could be relatedwith an increase of its regulator PA26 and high levels of proteasome-mediated proteolysis in vitro. Theintersection between the opposed peaks of 19S and 20S protein levels was marked by nuclear accumu-lation of both 20S and 19S together with Ubiquitin, suggesting a role of ubiquitin-proteasome system inthe nuclear protein turnover at the time. Our results revealed the importance of proteasome-mediatedproteolysis in T. cruzi IR-induced damage response suggesting that proteasome is also involved in T. cruziIR tolerance. Moreover, our data support the possible direct/signaling role of 19S in DNA damage repair.Based on these results, we speculate that spatial and temporal differences between the 19S particle and20S proteasome controls proteasome multiple roles in IR damage response. |
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Cerqueira, Paula GonçalvesSilva, Danielle Gomes PassosRocha, João Pedro Vieira daMendes, Isabela CeciliaOliveira, Karla Andrade deOliveira, Camila Franco Batista deVilela, Liza Figueiredo FelicoriNagem, Ronaldo Alves PintoCardoso, JosianeNardelli, Sheila CristinaKrieger, Marco AurélioFranco, Glória ReginaMacedo, Andréa MaraPena, Sérgio Danilo JunhoSchenkman, SérgioGomes, Dawidson AssisCota, Renata Guerra de SáMachado, Carlos Renato2017-12-20T16:55:44Z2017-12-20T16:55:44Z2017CERQUEIRA, P. G. et al. Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system. Molecular and Biochemical Parasitology, v. 212, p. 55-67, 2017. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0166685117300130?via%3Dihub>. Acesso em: 15 set. 2017.0166-6851http://www.repositorio.ufop.br/handle/123456789/9231https://doi.org/10.1016/j.molbiopara.2017.01.005tIn recent years, proteasome involvement in the damage response induced by ionizing radiation (IR)became evident. However, whether proteasome plays a direct or indirect role in IR-induced damageresponse still unclear. Trypanosoma cruzi is a human parasite capable of remarkable high tolerance toIR, suggesting a highly efficient damage response system. Here, we investigate the role of T. cruzi pro-teasome in the damage response induced by IR. We exposed epimastigotes to high doses of gamma rayand we analyzed the expression and subcellular localization of several components of the ubiquitin-proteasome system. We show that proteasome inhibition increases IR-induced cell growth arrest andproteasome-mediated proteolysis is altered after parasite exposure. We observed nuclear accumulationof 19S and 20S proteasome subunits in response to IR treatments. Intriguingly, the dynamic of 19S par-ticle nuclear accumulation was more similar to the dynamic observed for Rad51 nuclear translocationthan the observed for 20S. In the other hand, 20S increase and nuclear translocation could be relatedwith an increase of its regulator PA26 and high levels of proteasome-mediated proteolysis in vitro. Theintersection between the opposed peaks of 19S and 20S protein levels was marked by nuclear accumu-lation of both 20S and 19S together with Ubiquitin, suggesting a role of ubiquitin-proteasome system inthe nuclear protein turnover at the time. Our results revealed the importance of proteasome-mediatedproteolysis in T. cruzi IR-induced damage response suggesting that proteasome is also involved in T. cruziIR tolerance. Moreover, our data support the possible direct/signaling role of 19S in DNA damage repair.Based on these results, we speculate that spatial and temporal differences between the 19S particle and20S proteasome controls proteasome multiple roles in IR damage response.O periódico Molecular and Biochemical Parasitology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 4210810924923.info:eu-repo/semantics/openAccessEffect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-8924http://www.repositorio.ufop.br/bitstream/123456789/9231/2/license.txt62604f8d955274beb56c80ce1ee5dcaeMD52ORIGINALARTIGO_EffectIonizingRadiation.pdfARTIGO_EffectIonizingRadiation.pdfapplication/pdf3319408http://www.repositorio.ufop.br/bitstream/123456789/9231/1/ARTIGO_EffectIonizingRadiation.pdf99f7d7765aabaea284a5d26f20f90b36MD51123456789/92312020-02-20 10:11:52.338oai:localhost: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ório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332020-02-20T15:11:52Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system. |
title |
Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system. |
spellingShingle |
Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system. Cerqueira, Paula Gonçalves |
title_short |
Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system. |
title_full |
Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system. |
title_fullStr |
Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system. |
title_full_unstemmed |
Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system. |
title_sort |
Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system. |
author |
Cerqueira, Paula Gonçalves |
author_facet |
Cerqueira, Paula Gonçalves Silva, Danielle Gomes Passos Rocha, João Pedro Vieira da Mendes, Isabela Cecilia Oliveira, Karla Andrade de Oliveira, Camila Franco Batista de Vilela, Liza Figueiredo Felicori Nagem, Ronaldo Alves Pinto Cardoso, Josiane Nardelli, Sheila Cristina Krieger, Marco Aurélio Franco, Glória Regina Macedo, Andréa Mara Pena, Sérgio Danilo Junho Schenkman, Sérgio Gomes, Dawidson Assis Cota, Renata Guerra de Sá Machado, Carlos Renato |
author_role |
author |
author2 |
Silva, Danielle Gomes Passos Rocha, João Pedro Vieira da Mendes, Isabela Cecilia Oliveira, Karla Andrade de Oliveira, Camila Franco Batista de Vilela, Liza Figueiredo Felicori Nagem, Ronaldo Alves Pinto Cardoso, Josiane Nardelli, Sheila Cristina Krieger, Marco Aurélio Franco, Glória Regina Macedo, Andréa Mara Pena, Sérgio Danilo Junho Schenkman, Sérgio Gomes, Dawidson Assis Cota, Renata Guerra de Sá Machado, Carlos Renato |
author2_role |
author author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Cerqueira, Paula Gonçalves Silva, Danielle Gomes Passos Rocha, João Pedro Vieira da Mendes, Isabela Cecilia Oliveira, Karla Andrade de Oliveira, Camila Franco Batista de Vilela, Liza Figueiredo Felicori Nagem, Ronaldo Alves Pinto Cardoso, Josiane Nardelli, Sheila Cristina Krieger, Marco Aurélio Franco, Glória Regina Macedo, Andréa Mara Pena, Sérgio Danilo Junho Schenkman, Sérgio Gomes, Dawidson Assis Cota, Renata Guerra de Sá Machado, Carlos Renato |
description |
tIn recent years, proteasome involvement in the damage response induced by ionizing radiation (IR)became evident. However, whether proteasome plays a direct or indirect role in IR-induced damageresponse still unclear. Trypanosoma cruzi is a human parasite capable of remarkable high tolerance toIR, suggesting a highly efficient damage response system. Here, we investigate the role of T. cruzi pro-teasome in the damage response induced by IR. We exposed epimastigotes to high doses of gamma rayand we analyzed the expression and subcellular localization of several components of the ubiquitin-proteasome system. We show that proteasome inhibition increases IR-induced cell growth arrest andproteasome-mediated proteolysis is altered after parasite exposure. We observed nuclear accumulationof 19S and 20S proteasome subunits in response to IR treatments. Intriguingly, the dynamic of 19S par-ticle nuclear accumulation was more similar to the dynamic observed for Rad51 nuclear translocationthan the observed for 20S. In the other hand, 20S increase and nuclear translocation could be relatedwith an increase of its regulator PA26 and high levels of proteasome-mediated proteolysis in vitro. Theintersection between the opposed peaks of 19S and 20S protein levels was marked by nuclear accumu-lation of both 20S and 19S together with Ubiquitin, suggesting a role of ubiquitin-proteasome system inthe nuclear protein turnover at the time. Our results revealed the importance of proteasome-mediatedproteolysis in T. cruzi IR-induced damage response suggesting that proteasome is also involved in T. cruziIR tolerance. Moreover, our data support the possible direct/signaling role of 19S in DNA damage repair.Based on these results, we speculate that spatial and temporal differences between the 19S particle and20S proteasome controls proteasome multiple roles in IR damage response. |
publishDate |
2017 |
dc.date.accessioned.fl_str_mv |
2017-12-20T16:55:44Z |
dc.date.available.fl_str_mv |
2017-12-20T16:55:44Z |
dc.date.issued.fl_str_mv |
2017 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CERQUEIRA, P. G. et al. Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system. Molecular and Biochemical Parasitology, v. 212, p. 55-67, 2017. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0166685117300130?via%3Dihub>. Acesso em: 15 set. 2017. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/9231 |
dc.identifier.issn.none.fl_str_mv |
0166-6851 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.molbiopara.2017.01.005 |
identifier_str_mv |
CERQUEIRA, P. G. et al. Effect of ionizing radiation exposure on Trypanosoma cruzi ubiquitin-proteasome system. Molecular and Biochemical Parasitology, v. 212, p. 55-67, 2017. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0166685117300130?via%3Dihub>. Acesso em: 15 set. 2017. 0166-6851 |
url |
http://www.repositorio.ufop.br/handle/123456789/9231 https://doi.org/10.1016/j.molbiopara.2017.01.005 |
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eng |
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