The administration of surfactant decreased oxidative stress in lungs of mice exposed to cigarette smoke.
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/9894 https://www.sciencedirect.com/science/article/pii/S1567576917304435?via%3Dihub |
Resumo: | The alveolar surfactant, which composition consists of a unique and complex mixture of lipids and proteins, has immunomodulatory action. This study aimed to evaluate the effects of exogenous surfactant on pulmonary inflammatory response in mice exposed to cigarette smoke (CS). Twenty-four mice C57BL/6 were divided into four groups: control group exposed to ambient air (CG); surfactant treated group (SG); CS exposed group (CSG) and CS exposed group treated with surfactant (CSSG). For five days, CSG and CSSG were exposed to 12 commercial cigarettes/day and SG and CSSG received the surfactant by intranasal instillation. At the end of the experiment, the animals were euthanatized for the collection of bronchoalveolar lavage fluid (BALF) and lungs. The total number of leukocytes in BALF increased in CSG compared to CG, however, there was a decrease in CSSG compared to CSG. There was an increase in lipid peroxidation in SG and CSG compared to CG while there was a decrease in CSSG compared to CSG. Regarding the antioxidant enzymes, the catalase (CAT) activity increased in all groups compared to CG and the superoxide dismutase (SOD) activity decreased in CSG compared to the CG and SG. There was an increase in TNF in SG, CSG and CSSG compared to CG. There was an increase in IL-17 in CSSG compared to CG. There was an increase in CCL5 in SG and CSSG compared to CG. Therefore, our results demonstrated that the administration of exogenous surfactant was able to decrease the oxidative processes in the lungs of mice induced by short-term exposure to CS. |
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Machado, Dafne FernandesCampos, Keila Karine DuarteSilva, Natália Pereira daRamos, Camila de OliveiraCangussú, Silvia DantasCosta, Guilherme de PaulaSilva, André Talvani Pedrosa daBezerra, Frank Silva2018-05-03T14:48:45Z2018-05-03T14:48:45Z2018MACHADO, D. F. et al. The administration of surfactant decreased oxidative stress in lungs of mice exposed to cigarette smoke. International Immunopharmacology, v. 54, p. 275-279, 2018. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1567576917304435?via%3Dihub>. Acesso em: 05 abr. 2018. 15675769http://www.repositorio.ufop.br/handle/123456789/9894https://www.sciencedirect.com/science/article/pii/S1567576917304435?via%3DihubThe alveolar surfactant, which composition consists of a unique and complex mixture of lipids and proteins, has immunomodulatory action. This study aimed to evaluate the effects of exogenous surfactant on pulmonary inflammatory response in mice exposed to cigarette smoke (CS). Twenty-four mice C57BL/6 were divided into four groups: control group exposed to ambient air (CG); surfactant treated group (SG); CS exposed group (CSG) and CS exposed group treated with surfactant (CSSG). For five days, CSG and CSSG were exposed to 12 commercial cigarettes/day and SG and CSSG received the surfactant by intranasal instillation. At the end of the experiment, the animals were euthanatized for the collection of bronchoalveolar lavage fluid (BALF) and lungs. The total number of leukocytes in BALF increased in CSG compared to CG, however, there was a decrease in CSSG compared to CSG. There was an increase in lipid peroxidation in SG and CSG compared to CG while there was a decrease in CSSG compared to CSG. Regarding the antioxidant enzymes, the catalase (CAT) activity increased in all groups compared to CG and the superoxide dismutase (SOD) activity decreased in CSG compared to the CG and SG. There was an increase in TNF in SG, CSG and CSSG compared to CG. There was an increase in IL-17 in CSSG compared to CG. There was an increase in CCL5 in SG and CSSG compared to CG. Therefore, our results demonstrated that the administration of exogenous surfactant was able to decrease the oxidative processes in the lungs of mice induced by short-term exposure to CS.Cigarette smokeLung inflammationRedox imbalanceExogeneous surfactantThe administration of surfactant decreased oxidative stress in lungs of mice exposed to cigarette smoke.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-8924http://www.repositorio.ufop.br/bitstream/123456789/9894/2/license.txt62604f8d955274beb56c80ce1ee5dcaeMD52ORIGINALARTIGO_AdministrationSurfactantDecreased.pdfARTIGO_AdministrationSurfactantDecreased.pdfapplication/pdf307520http://www.repositorio.ufop.br/bitstream/123456789/9894/1/ARTIGO_AdministrationSurfactantDecreased.pdf19a37b708f42be5f24d08b32cc527cfeMD51123456789/98942018-05-03 10:48:45.275oai:localhost: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ório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332018-05-03T14:48:45Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
The administration of surfactant decreased oxidative stress in lungs of mice exposed to cigarette smoke. |
title |
The administration of surfactant decreased oxidative stress in lungs of mice exposed to cigarette smoke. |
spellingShingle |
The administration of surfactant decreased oxidative stress in lungs of mice exposed to cigarette smoke. Machado, Dafne Fernandes Cigarette smoke Lung inflammation Redox imbalance Exogeneous surfactant |
title_short |
The administration of surfactant decreased oxidative stress in lungs of mice exposed to cigarette smoke. |
title_full |
The administration of surfactant decreased oxidative stress in lungs of mice exposed to cigarette smoke. |
title_fullStr |
The administration of surfactant decreased oxidative stress in lungs of mice exposed to cigarette smoke. |
title_full_unstemmed |
The administration of surfactant decreased oxidative stress in lungs of mice exposed to cigarette smoke. |
title_sort |
The administration of surfactant decreased oxidative stress in lungs of mice exposed to cigarette smoke. |
author |
Machado, Dafne Fernandes |
author_facet |
Machado, Dafne Fernandes Campos, Keila Karine Duarte Silva, Natália Pereira da Ramos, Camila de Oliveira Cangussú, Silvia Dantas Costa, Guilherme de Paula Silva, André Talvani Pedrosa da Bezerra, Frank Silva |
author_role |
author |
author2 |
Campos, Keila Karine Duarte Silva, Natália Pereira da Ramos, Camila de Oliveira Cangussú, Silvia Dantas Costa, Guilherme de Paula Silva, André Talvani Pedrosa da Bezerra, Frank Silva |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Machado, Dafne Fernandes Campos, Keila Karine Duarte Silva, Natália Pereira da Ramos, Camila de Oliveira Cangussú, Silvia Dantas Costa, Guilherme de Paula Silva, André Talvani Pedrosa da Bezerra, Frank Silva |
dc.subject.por.fl_str_mv |
Cigarette smoke Lung inflammation Redox imbalance Exogeneous surfactant |
topic |
Cigarette smoke Lung inflammation Redox imbalance Exogeneous surfactant |
description |
The alveolar surfactant, which composition consists of a unique and complex mixture of lipids and proteins, has immunomodulatory action. This study aimed to evaluate the effects of exogenous surfactant on pulmonary inflammatory response in mice exposed to cigarette smoke (CS). Twenty-four mice C57BL/6 were divided into four groups: control group exposed to ambient air (CG); surfactant treated group (SG); CS exposed group (CSG) and CS exposed group treated with surfactant (CSSG). For five days, CSG and CSSG were exposed to 12 commercial cigarettes/day and SG and CSSG received the surfactant by intranasal instillation. At the end of the experiment, the animals were euthanatized for the collection of bronchoalveolar lavage fluid (BALF) and lungs. The total number of leukocytes in BALF increased in CSG compared to CG, however, there was a decrease in CSSG compared to CSG. There was an increase in lipid peroxidation in SG and CSG compared to CG while there was a decrease in CSSG compared to CSG. Regarding the antioxidant enzymes, the catalase (CAT) activity increased in all groups compared to CG and the superoxide dismutase (SOD) activity decreased in CSG compared to the CG and SG. There was an increase in TNF in SG, CSG and CSSG compared to CG. There was an increase in IL-17 in CSSG compared to CG. There was an increase in CCL5 in SG and CSSG compared to CG. Therefore, our results demonstrated that the administration of exogenous surfactant was able to decrease the oxidative processes in the lungs of mice induced by short-term exposure to CS. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-05-03T14:48:45Z |
dc.date.available.fl_str_mv |
2018-05-03T14:48:45Z |
dc.date.issued.fl_str_mv |
2018 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MACHADO, D. F. et al. The administration of surfactant decreased oxidative stress in lungs of mice exposed to cigarette smoke. International Immunopharmacology, v. 54, p. 275-279, 2018. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1567576917304435?via%3Dihub>. Acesso em: 05 abr. 2018. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/9894 |
dc.identifier.issn.none.fl_str_mv |
15675769 |
dc.identifier.uri2.pt_BR.fl_str_mv |
https://www.sciencedirect.com/science/article/pii/S1567576917304435?via%3Dihub |
identifier_str_mv |
MACHADO, D. F. et al. The administration of surfactant decreased oxidative stress in lungs of mice exposed to cigarette smoke. International Immunopharmacology, v. 54, p. 275-279, 2018. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1567576917304435?via%3Dihub>. Acesso em: 05 abr. 2018. 15675769 |
url |
http://www.repositorio.ufop.br/handle/123456789/9894 https://www.sciencedirect.com/science/article/pii/S1567576917304435?via%3Dihub |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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reponame:Repositório Institucional da UFOP instname:Universidade Federal de Ouro Preto (UFOP) instacron:UFOP |
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Universidade Federal de Ouro Preto (UFOP) |
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UFOP |
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