Methylmercury inhibits prolactin release in a cell line of pituitary origin
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFPA |
Texto Completo: | http://repositorio.ufpa.br/jspui/handle/2011/8442 http://dx.doi.org/10.1590/1414-431X20154165 |
Resumo: | Heavy metals, such as methylmercury, are key environmental pollutants that easily reach human beings by bioaccumulation through the food chain. Several reports have demonstrated that endocrine organs, and especially the pituitary gland, are potential targets for mercury accumulation; however, the effects on the regulation of hormonal release are unclear. It has been suggested that serum prolactin could represent a biomarker of heavy metal exposure. The aim of this study was to evaluate the effect of methylmercury on prolactin release and the role of the nitrergic system using prolactin secretory cells (the mammosomatotroph cell line, GH3B6). Exposure to methylmercury (0-100 μM) was cytotoxic in a time- and concentration-dependent manner, with an LC50 higher than described for cells of neuronal origin, suggesting GH3B6 cells have a relative resistance. Methylmercury (at exposures as low as 1 μM for 2 h) also decreased prolactin release. Interestingly, inhibition of nitric oxide synthase by N-nitro-L-arginine completely prevented the decrease in prolactin release without acute neurotoxic effects of methylmercury. These data indicate that the decrease in prolactin production occurs via activation of the nitrergic system and is an early effect of methylmercury in cells of pituitary origin. |
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2017-05-17T16:37:47Z2017-05-17T16:37:47Z2015-08MAUES, Luís Antônio Loureiro et al. Methylmercury inhibits prolactin release in a cell line of pituitary origin. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 48, n. 8, p. 691-696, ago. 2015. Disponível em: <http://repositorio.ufpa.br/jspui/handle/2011/8442>. Acesso em:.1414-431Xhttp://repositorio.ufpa.br/jspui/handle/2011/8442http://dx.doi.org/10.1590/1414-431X20154165Heavy metals, such as methylmercury, are key environmental pollutants that easily reach human beings by bioaccumulation through the food chain. Several reports have demonstrated that endocrine organs, and especially the pituitary gland, are potential targets for mercury accumulation; however, the effects on the regulation of hormonal release are unclear. It has been suggested that serum prolactin could represent a biomarker of heavy metal exposure. The aim of this study was to evaluate the effect of methylmercury on prolactin release and the role of the nitrergic system using prolactin secretory cells (the mammosomatotroph cell line, GH3B6). Exposure to methylmercury (0-100 μM) was cytotoxic in a time- and concentration-dependent manner, with an LC50 higher than described for cells of neuronal origin, suggesting GH3B6 cells have a relative resistance. Methylmercury (at exposures as low as 1 μM for 2 h) also decreased prolactin release. Interestingly, inhibition of nitric oxide synthase by N-nitro-L-arginine completely prevented the decrease in prolactin release without acute neurotoxic effects of methylmercury. These data indicate that the decrease in prolactin production occurs via activation of the nitrergic system and is an early effect of methylmercury in cells of pituitary origin.MAUÉS, L. A. L. Dr. Docente da Universidade Federal do Pará, Campus Universitário de AltamiraMACCHI, B. de M. Dr. Docente da Universidade Federal do Pará, Instituto de Ciências BiológicasCRESPO LÓPEZ, M. E. Dr. Docente da Universidade Federal do Pará, Insituto de Ciências BiológicasporUniversidade Federal do ParáUFPABrasilBrazilian Journal of Medical and Biological Researchhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800691&lng=pt&nrm=isoreponame:Repositório Institucional da UFPAinstname:Universidade Federal do Pará (UFPA)instacron:UFPAMetilmercúrioProlactinaMetais pesadosCélulas secretorasPituitáriaMercúrioMethylmercury inhibits prolactin release in a cell line of pituitary origininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article488691696http://lattes.cnpq.br/4851018582496177http://lattes.cnpq.br/5330351659478942http://lattes.cnpq.br/9900144256348265http://lattes.cnpq.br/8341603979365998http://lattes.cnpq.br/9601463988942971MAUÉS, Luis Antônio LoureiroMACCHI, Barbarella de MatosCRESPO LÓPEZ, Maria ElenaNASCIUTTI, Luís EuricoDINIZ, Domingos Luiz Wanderley Picançoinfo:eu-repo/semantics/openAccessTEXTArtigo_MethylmercuryInhibitsProlactin.pdf.txtArtigo_MethylmercuryInhibitsProlactin.pdf.txtExtracted texttext/plain25195http://repositorio.ufpa.br/oai/bitstream/2011/8442/6/Artigo_MethylmercuryInhibitsProlactin.pdf.txte606928753203527f35874d58a12068dMD56ORIGINALArtigo_MethylmercuryInhibitsProlactin.pdfArtigo_MethylmercuryInhibitsProlactin.pdfapplication/pdf439200http://repositorio.ufpa.br/oai/bitstream/2011/8442/1/Artigo_MethylmercuryInhibitsProlactin.pdfe9b746f6932082a43e3916b16a6956abMD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.ufpa.br/oai/bitstream/2011/8442/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://repositorio.ufpa.br/oai/bitstream/2011/8442/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://repositorio.ufpa.br/oai/bitstream/2011/8442/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81866http://repositorio.ufpa.br/oai/bitstream/2011/8442/5/license.txt43cd690d6a359e86c1fe3d5b7cba0c9bMD552011/84422018-05-16 14:44:28.107oai:repositorio.ufpa.br:2011/8442TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkNvbSBhIGFwcmVzZW50YcOnw6NvIGRlc3RhIGxpY2Vuw6dhLCB2b2PDqiAobyBhdXRvciAoZXMpIG91IG8gdGl0dWxhciBkb3MgZGlyZWl0b3MgZGUgYXV0b3IpIGNvbmNlZGUgYW8gUmVwb3NpdMOzcmlvIApJbnN0aXR1Y2lvbmFsIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSByZXByb2R1emlyLCAgdHJhZHV6aXIgKGNvbmZvcm1lIGRlZmluaWRvIGFiYWl4byksIGUvb3UgZGlzdHJpYnVpciBhIApzdWEgcHVibGljYcOnw6NvIChpbmNsdWluZG8gbyByZXN1bW8pIHBvciB0b2RvIG8gbXVuZG8gbm8gZm9ybWF0byBpbXByZXNzbyBlIGVsZXRyw7RuaWNvIGUgZW0gcXVhbHF1ZXIgbWVpbywgaW5jbHVpbmRvIG9zIApmb3JtYXRvcyDDoXVkaW8gb3UgdsOtZGVvLgoKVm9jw6ogY29uY29yZGEgcXVlIG8gRGVwb3NpdGEgcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250ZcO6ZG8sIHRyYW5zcG9yIGEgc3VhIHB1YmxpY2HDp8OjbyBwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byAKcGFyYSBmaW5zIGRlIHByZXNlcnZhw6fDo28uCgpWb2PDqiB0YW1iw6ltIGNvbmNvcmRhIHF1ZSBvIERlcG9zaXRhIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPDs3BpYSBkZSBzdWEgcHVibGljYcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIAplIHByZXNlcnZhw6fDo28uCgpWb2PDqiBkZWNsYXJhIHF1ZSBhIHN1YSBwdWJsaWNhw6fDo28gw6kgb3JpZ2luYWwgZSBxdWUgdm9jw6ogdGVtIG8gcG9kZXIgZGUgY29uY2VkZXIgb3MgZGlyZWl0b3MgY29udGlkb3MgbmVzdGEgbGljZW7Dp2EuIApWb2PDqiB0YW1iw6ltIGRlY2xhcmEgcXVlIG8gZGVww7NzaXRvIGRhIHN1YSBwdWJsaWNhw6fDo28gbsOjbywgcXVlIHNlamEgZGUgc2V1IGNvbmhlY2ltZW50bywgaW5mcmluZ2UgZGlyZWl0b3MgYXV0b3JhaXMgCmRlIG5pbmd1w6ltLgoKQ2FzbyBhIHN1YSBwdWJsaWNhw6fDo28gY29udGVuaGEgbWF0ZXJpYWwgcXVlIHZvY8OqIG7Do28gcG9zc3VpIGEgdGl0dWxhcmlkYWRlIGRvcyBkaXJlaXRvcyBhdXRvcmFpcywgdm9jw6ogZGVjbGFyYSBxdWUgCm9idGV2ZSBhIHBlcm1pc3PDo28gaXJyZXN0cml0YSBkbyBkZXRlbnRvciBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgcGFyYSBjb25jZWRlciBhbyBEZXBvc2l0YSBvcyBkaXJlaXRvcyBhcHJlc2VudGFkb3MgCm5lc3RhIGxpY2Vuw6dhLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIG5vIHRleHRvIApvdSBubyBjb250ZcO6ZG8gZGEgcHVibGljYcOnw6NvIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFBVQkxJQ0HDh8ODTyBPUkEgREVQT1NJVEFEQSBURU5IQSBTSURPIFJFU1VMVEFETyBERSBVTSBQQVRST0PDjU5JTyBPVSBBUE9JTyBERSBVTUEgQUfDik5DSUEgREUgRk9NRU5UTyBPVSBPVVRSTyAKT1JHQU5JU01PLCBWT0PDiiBERUNMQVJBIFFVRSBSRVNQRUlUT1UgVE9ET1MgRSBRVUFJU1FVRVIgRElSRUlUT1MgREUgUkVWSVPDg08gQ09NTyBUQU1Cw4lNIEFTIERFTUFJUyBPQlJJR0HDh8OVRVMgCkVYSUdJREFTIFBPUiBDT05UUkFUTyBPVSBBQ09SRE8uCgpPIERlcG9zaXRhIHNlIGNvbXByb21ldGUgYSBpZGVudGlmaWNhciBjbGFyYW1lbnRlIG8gc2V1IG5vbWUgKHMpIG91IG8ocykgbm9tZShzKSBkbyhzKSBkZXRlbnRvcihlcykgZG9zIGRpcmVpdG9zIAphdXRvcmFpcyBkYSBwdWJsaWNhw6fDo28sIGUgbsOjbyBmYXLDoSBxdWFscXVlciBhbHRlcmHDp8OjbywgYWzDqW0gZGFxdWVsYXMgY29uY2VkaWRhcyBwb3IgZXN0YSBsaWNlbsOnYS4KRepositório InstitucionalPUBhttp://repositorio.ufpa.br/oai/requestriufpabc@ufpa.bropendoar:21232018-05-16T17:44:28Repositório Institucional da UFPA - Universidade Federal do Pará (UFPA)false |
dc.title.pt_BR.fl_str_mv |
Methylmercury inhibits prolactin release in a cell line of pituitary origin |
title |
Methylmercury inhibits prolactin release in a cell line of pituitary origin |
spellingShingle |
Methylmercury inhibits prolactin release in a cell line of pituitary origin MAUÉS, Luis Antônio Loureiro Metilmercúrio Prolactina Metais pesados Células secretoras Pituitária Mercúrio |
title_short |
Methylmercury inhibits prolactin release in a cell line of pituitary origin |
title_full |
Methylmercury inhibits prolactin release in a cell line of pituitary origin |
title_fullStr |
Methylmercury inhibits prolactin release in a cell line of pituitary origin |
title_full_unstemmed |
Methylmercury inhibits prolactin release in a cell line of pituitary origin |
title_sort |
Methylmercury inhibits prolactin release in a cell line of pituitary origin |
author |
MAUÉS, Luis Antônio Loureiro |
author_facet |
MAUÉS, Luis Antônio Loureiro MACCHI, Barbarella de Matos CRESPO LÓPEZ, Maria Elena NASCIUTTI, Luís Eurico DINIZ, Domingos Luiz Wanderley Picanço |
author_role |
author |
author2 |
MACCHI, Barbarella de Matos CRESPO LÓPEZ, Maria Elena NASCIUTTI, Luís Eurico DINIZ, Domingos Luiz Wanderley Picanço |
author2_role |
author author author author |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4851018582496177 http://lattes.cnpq.br/5330351659478942 http://lattes.cnpq.br/9900144256348265 http://lattes.cnpq.br/8341603979365998 http://lattes.cnpq.br/9601463988942971 |
dc.contributor.author.fl_str_mv |
MAUÉS, Luis Antônio Loureiro MACCHI, Barbarella de Matos CRESPO LÓPEZ, Maria Elena NASCIUTTI, Luís Eurico DINIZ, Domingos Luiz Wanderley Picanço |
dc.subject.por.fl_str_mv |
Metilmercúrio Prolactina Metais pesados Células secretoras Pituitária Mercúrio |
topic |
Metilmercúrio Prolactina Metais pesados Células secretoras Pituitária Mercúrio |
description |
Heavy metals, such as methylmercury, are key environmental pollutants that easily reach human beings by bioaccumulation through the food chain. Several reports have demonstrated that endocrine organs, and especially the pituitary gland, are potential targets for mercury accumulation; however, the effects on the regulation of hormonal release are unclear. It has been suggested that serum prolactin could represent a biomarker of heavy metal exposure. The aim of this study was to evaluate the effect of methylmercury on prolactin release and the role of the nitrergic system using prolactin secretory cells (the mammosomatotroph cell line, GH3B6). Exposure to methylmercury (0-100 μM) was cytotoxic in a time- and concentration-dependent manner, with an LC50 higher than described for cells of neuronal origin, suggesting GH3B6 cells have a relative resistance. Methylmercury (at exposures as low as 1 μM for 2 h) also decreased prolactin release. Interestingly, inhibition of nitric oxide synthase by N-nitro-L-arginine completely prevented the decrease in prolactin release without acute neurotoxic effects of methylmercury. These data indicate that the decrease in prolactin production occurs via activation of the nitrergic system and is an early effect of methylmercury in cells of pituitary origin. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-08 |
dc.date.accessioned.fl_str_mv |
2017-05-17T16:37:47Z |
dc.date.available.fl_str_mv |
2017-05-17T16:37:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MAUES, Luís Antônio Loureiro et al. Methylmercury inhibits prolactin release in a cell line of pituitary origin. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 48, n. 8, p. 691-696, ago. 2015. Disponível em: <http://repositorio.ufpa.br/jspui/handle/2011/8442>. Acesso em:. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpa.br/jspui/handle/2011/8442 |
dc.identifier.issn.pt_BR.fl_str_mv |
1414-431X |
dc.identifier.doi.pt_BR.fl_str_mv |
http://dx.doi.org/10.1590/1414-431X20154165 |
identifier_str_mv |
MAUES, Luís Antônio Loureiro et al. Methylmercury inhibits prolactin release in a cell line of pituitary origin. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 48, n. 8, p. 691-696, ago. 2015. Disponível em: <http://repositorio.ufpa.br/jspui/handle/2011/8442>. Acesso em:. 1414-431X |
url |
http://repositorio.ufpa.br/jspui/handle/2011/8442 http://dx.doi.org/10.1590/1414-431X20154165 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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Brazilian Journal of Medical and Biological Research |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal do Pará |
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UFPA |
dc.publisher.country.fl_str_mv |
Brasil |
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Universidade Federal do Pará |
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