Cytogenetic studies in six species of Scinax (Anura, Hylidae) clade Scinax ruber from northern and northeastern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFPA |
Texto Completo: | http://repositorio.ufpa.br/jspui/handle/2011/6871 |
Resumo: | Scinax species are still underrepresented in cytogenetic studies, mainly with respect to populations from northeastern and northern Brazil. In this study, we provide new chromosomal information on Scinax boesemani, S. camposseabrai, S. garbei, S. pachycrus, S. trilineatus and S. x-signatus, all belonging to clade S. ruber. They were collected at two locations in the Caatinga biome (northeastern Brazil) and at one in the Amazon (northern Brazil) biomes. Chromosomes were analyzed by conventional staining, C-banding, Ag-NOR staining, and fluorochrome staining. All species shared a modal diploid value of 2n = 24 and fundamental arm number (FN) of 48. Moreover, both chromosomal size and morphology were similar to other species in this Scinax clade. C-banding revealed centromeric heterochromatin in all species, along with terminal species-specific C-bands in some species. Active nucleolar organizer regions (Ag-NORs) were identified at 11q in most species, except for S. boesemani and S. garbei (Ag-NORs at interstitial region of 8q). Differing from most anurans, GC-rich regions were not restricted to NORs, but also coincident with some centromeric and terminal C-bands. These data contribute to the cytotaxonomy of Scinax by providing chromosomal markers and demonstrating the occurrence of microstructural rearrangements and inversions on chromosomal evolution of Scinax. |
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2015-09-10T13:05:26Z2015-09-10T13:05:26Z2015-06SILVA, Lídia Nogueira et al. Cytogenetic studies in six species of Scinax (Anura, Hylidae) clade Scinax ruber from northern and northeastern Brazil. Genetics and Molecular Biology, Ribeirão Preto, v. 38, n. 2, p. 156-161, jun. 2015. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200156&lng=pt&nrm=iso>. Acesso em: 24 ago. 2015. <http://dx.doi.org/10.1590/S1415-4757382220140280>.1678-4685http://repositorio.ufpa.br/jspui/handle/2011/6871Scinax species are still underrepresented in cytogenetic studies, mainly with respect to populations from northeastern and northern Brazil. In this study, we provide new chromosomal information on Scinax boesemani, S. camposseabrai, S. garbei, S. pachycrus, S. trilineatus and S. x-signatus, all belonging to clade S. ruber. They were collected at two locations in the Caatinga biome (northeastern Brazil) and at one in the Amazon (northern Brazil) biomes. Chromosomes were analyzed by conventional staining, C-banding, Ag-NOR staining, and fluorochrome staining. All species shared a modal diploid value of 2n = 24 and fundamental arm number (FN) of 48. Moreover, both chromosomal size and morphology were similar to other species in this Scinax clade. C-banding revealed centromeric heterochromatin in all species, along with terminal species-specific C-bands in some species. Active nucleolar organizer regions (Ag-NORs) were identified at 11q in most species, except for S. boesemani and S. garbei (Ag-NORs at interstitial region of 8q). Differing from most anurans, GC-rich regions were not restricted to NORs, but also coincident with some centromeric and terminal C-bands. These data contribute to the cytotaxonomy of Scinax by providing chromosomal markers and demonstrating the occurrence of microstructural rearrangements and inversions on chromosomal evolution of Scinax.engAnfíbioCitogenéticaDNACromossomos de anurosCytogenetic studies in six species of Scinax (Anura, Hylidae) clade Scinax ruber from northern and northeastern Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSILVA, Lídia NogueiraZANONI, Juliani BrunaSOLÉ, MircoAFFONSO, Paulo Roberto Antunes de MelloSIQUEIRA, SérgioSAMPAIO, Maria Iracilda da Cunhainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPAinstname:Universidade Federal do Pará (UFPA)instacron:UFPACC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.ufpa.br/oai/bitstream/2011/6871/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-821328http://repositorio.ufpa.br/oai/bitstream/2011/6871/3/license_text1ddceb2a2635a9472b3c10d731d0906eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-822190http://repositorio.ufpa.br/oai/bitstream/2011/6871/4/license_rdf19e8a2b57ef43c09f4d7071d2153c97dMD54ORIGINALArtigo_CytogeneticStudiesSix.pdfArtigo_CytogeneticStudiesSix.pdfapplication/pdf1627319http://repositorio.ufpa.br/oai/bitstream/2011/6871/1/Artigo_CytogeneticStudiesSix.pdfb2e7972ddab073a084a790de725ee9f3MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81703http://repositorio.ufpa.br/oai/bitstream/2011/6871/5/license.txta12ee01655d4f43dacf016d5e6168febMD55TEXTArtigo_CytogeneticStudiesSix.pdf.txtArtigo_CytogeneticStudiesSix.pdf.txtExtracted texttext/plain21232http://repositorio.ufpa.br/oai/bitstream/2011/6871/6/Artigo_CytogeneticStudiesSix.pdf.txt5d6a6abb78f129bcdec5dcf81ec64835MD562011/68712017-12-14 10:26:25.106oai:repositorio.ufpa.br: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Repositório InstitucionalPUBhttp://repositorio.ufpa.br/oai/requestriufpabc@ufpa.bropendoar:21232017-12-14T13:26:25Repositório Institucional da UFPA - Universidade Federal do Pará (UFPA)false |
dc.title.pt_BR.fl_str_mv |
Cytogenetic studies in six species of Scinax (Anura, Hylidae) clade Scinax ruber from northern and northeastern Brazil |
title |
Cytogenetic studies in six species of Scinax (Anura, Hylidae) clade Scinax ruber from northern and northeastern Brazil |
spellingShingle |
Cytogenetic studies in six species of Scinax (Anura, Hylidae) clade Scinax ruber from northern and northeastern Brazil SILVA, Lídia Nogueira Anfíbio Citogenética DNA Cromossomos de anuros |
title_short |
Cytogenetic studies in six species of Scinax (Anura, Hylidae) clade Scinax ruber from northern and northeastern Brazil |
title_full |
Cytogenetic studies in six species of Scinax (Anura, Hylidae) clade Scinax ruber from northern and northeastern Brazil |
title_fullStr |
Cytogenetic studies in six species of Scinax (Anura, Hylidae) clade Scinax ruber from northern and northeastern Brazil |
title_full_unstemmed |
Cytogenetic studies in six species of Scinax (Anura, Hylidae) clade Scinax ruber from northern and northeastern Brazil |
title_sort |
Cytogenetic studies in six species of Scinax (Anura, Hylidae) clade Scinax ruber from northern and northeastern Brazil |
author |
SILVA, Lídia Nogueira |
author_facet |
SILVA, Lídia Nogueira ZANONI, Juliani Bruna SOLÉ, Mirco AFFONSO, Paulo Roberto Antunes de Mello SIQUEIRA, Sérgio SAMPAIO, Maria Iracilda da Cunha |
author_role |
author |
author2 |
ZANONI, Juliani Bruna SOLÉ, Mirco AFFONSO, Paulo Roberto Antunes de Mello SIQUEIRA, Sérgio SAMPAIO, Maria Iracilda da Cunha |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
SILVA, Lídia Nogueira ZANONI, Juliani Bruna SOLÉ, Mirco AFFONSO, Paulo Roberto Antunes de Mello SIQUEIRA, Sérgio SAMPAIO, Maria Iracilda da Cunha |
dc.subject.por.fl_str_mv |
Anfíbio Citogenética DNA Cromossomos de anuros |
topic |
Anfíbio Citogenética DNA Cromossomos de anuros |
description |
Scinax species are still underrepresented in cytogenetic studies, mainly with respect to populations from northeastern and northern Brazil. In this study, we provide new chromosomal information on Scinax boesemani, S. camposseabrai, S. garbei, S. pachycrus, S. trilineatus and S. x-signatus, all belonging to clade S. ruber. They were collected at two locations in the Caatinga biome (northeastern Brazil) and at one in the Amazon (northern Brazil) biomes. Chromosomes were analyzed by conventional staining, C-banding, Ag-NOR staining, and fluorochrome staining. All species shared a modal diploid value of 2n = 24 and fundamental arm number (FN) of 48. Moreover, both chromosomal size and morphology were similar to other species in this Scinax clade. C-banding revealed centromeric heterochromatin in all species, along with terminal species-specific C-bands in some species. Active nucleolar organizer regions (Ag-NORs) were identified at 11q in most species, except for S. boesemani and S. garbei (Ag-NORs at interstitial region of 8q). Differing from most anurans, GC-rich regions were not restricted to NORs, but also coincident with some centromeric and terminal C-bands. These data contribute to the cytotaxonomy of Scinax by providing chromosomal markers and demonstrating the occurrence of microstructural rearrangements and inversions on chromosomal evolution of Scinax. |
publishDate |
2015 |
dc.date.accessioned.fl_str_mv |
2015-09-10T13:05:26Z |
dc.date.available.fl_str_mv |
2015-09-10T13:05:26Z |
dc.date.issued.fl_str_mv |
2015-06 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Lídia Nogueira et al. Cytogenetic studies in six species of Scinax (Anura, Hylidae) clade Scinax ruber from northern and northeastern Brazil. Genetics and Molecular Biology, Ribeirão Preto, v. 38, n. 2, p. 156-161, jun. 2015. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200156&lng=pt&nrm=iso>. Acesso em: 24 ago. 2015. <http://dx.doi.org/10.1590/S1415-4757382220140280>. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpa.br/jspui/handle/2011/6871 |
dc.identifier.issn.none.fl_str_mv |
1678-4685 |
identifier_str_mv |
SILVA, Lídia Nogueira et al. Cytogenetic studies in six species of Scinax (Anura, Hylidae) clade Scinax ruber from northern and northeastern Brazil. Genetics and Molecular Biology, Ribeirão Preto, v. 38, n. 2, p. 156-161, jun. 2015. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200156&lng=pt&nrm=iso>. Acesso em: 24 ago. 2015. <http://dx.doi.org/10.1590/S1415-4757382220140280>. 1678-4685 |
url |
http://repositorio.ufpa.br/jspui/handle/2011/6871 |
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eng |
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eng |
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openAccess |
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