Oxidative stress and antioxidant status in beta-thalassemia heterozygotes
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFPA |
Texto Completo: | http://repositorio.ufpa.br/jspui/handle/2011/6126 |
Resumo: | Background: Several studies have evaluated the oxidant and antioxidant status of thalassemia patients but most focused mainly on the severe and intermediate states of the disease. Moreover, the oxidative status has not been evaluated for the different beta-thalassemia mutations. Objective: To evaluate lipid peroxidation and Trolox equivalent antioxidant capacity in relation to serum iron and ferritin in beta thalassemia resulting from two different mutations (CD39 and IVS-I-110) compared to individuals without beta-thalassemia. Methods: One hundred and thirty subjects were studied, including 49 who were heterozygous for beta-thalassemia and 81 controls. Blood samples were subjected to screening tests for hemoglobin. Allele-specific polymerase chain reaction was used to confirm mutations for beta-thalassemia, an analysis of thiobarbituric acid reactive species was used to determine lipid peroxidation, and Trolox equivalent antioxidant capacity evaluations were performed. The heterozygous beta-thalassemia group was also evaluated for serum iron and ferritin status. Results: Thiobarbituric acid reactive species (486.24 ± 119.64 ng/mL) and Trolox equivalent antioxidant capacity values (2.23 ± 0.11 mM/L) were higher in beta-thalassemia heterozygotes compared to controls (260.86 ± 92.40 ng/mL and 2.12 ± 0.10 mM/L, respectively; p-value < 0.01). Increased thiobarbituric acid reactive species values were observed in subjects with the CD39 mutation compared with those with the IVS-I-110 mutation (529.94 ± 115.60 ng/mL and 453.39 ± 121.10 ng/mL, respectively; p-value = 0.04). However, average Trolox equivalent antioxidant capacity values were similar for both mutations (2.20 ± 0.08 mM/L and 2.23 ± 0.12 mM/L, respectively; p-value = 0.39). There was no influence of serum iron and ferritin levels on thiobarbituric acid reactive species and Trolox equivalent antioxidant capacity values. Conclusion: This study shows an increase of oxidative stress and antioxidant capacity in beta-thalassemia heterozygotes, mainly in carriers of the CD39 mutation. |
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2014-11-28T13:02:41Z2014-11-28T13:02:41Z2013-12ONDEI, Luciana de Souza et al. Oxidative stress and antioxidant status in beta-thalassemia heterozygotes. Revista Brasileira de Hematologia e Hemoterapia, São José do Rio Preto, v. 35, n. 6, p. 409-413, nov./dez. 2013. Disponível em: <http://www.scielo.br/pdf/rbhh/v35n6/1516-8484-rbhh-35-06-0409.pdf>. Acesso em: 27 nov. 2014. <http://dx.doi.org/10.5581/1516-8484.20130122>.1516-8484http://repositorio.ufpa.br/jspui/handle/2011/6126Background: Several studies have evaluated the oxidant and antioxidant status of thalassemia patients but most focused mainly on the severe and intermediate states of the disease. Moreover, the oxidative status has not been evaluated for the different beta-thalassemia mutations. Objective: To evaluate lipid peroxidation and Trolox equivalent antioxidant capacity in relation to serum iron and ferritin in beta thalassemia resulting from two different mutations (CD39 and IVS-I-110) compared to individuals without beta-thalassemia. Methods: One hundred and thirty subjects were studied, including 49 who were heterozygous for beta-thalassemia and 81 controls. Blood samples were subjected to screening tests for hemoglobin. Allele-specific polymerase chain reaction was used to confirm mutations for beta-thalassemia, an analysis of thiobarbituric acid reactive species was used to determine lipid peroxidation, and Trolox equivalent antioxidant capacity evaluations were performed. The heterozygous beta-thalassemia group was also evaluated for serum iron and ferritin status. Results: Thiobarbituric acid reactive species (486.24 ± 119.64 ng/mL) and Trolox equivalent antioxidant capacity values (2.23 ± 0.11 mM/L) were higher in beta-thalassemia heterozygotes compared to controls (260.86 ± 92.40 ng/mL and 2.12 ± 0.10 mM/L, respectively; p-value < 0.01). Increased thiobarbituric acid reactive species values were observed in subjects with the CD39 mutation compared with those with the IVS-I-110 mutation (529.94 ± 115.60 ng/mL and 453.39 ± 121.10 ng/mL, respectively; p-value = 0.04). However, average Trolox equivalent antioxidant capacity values were similar for both mutations (2.20 ± 0.08 mM/L and 2.23 ± 0.12 mM/L, respectively; p-value = 0.39). There was no influence of serum iron and ferritin levels on thiobarbituric acid reactive species and Trolox equivalent antioxidant capacity values. Conclusion: This study shows an increase of oxidative stress and antioxidant capacity in beta-thalassemia heterozygotes, mainly in carriers of the CD39 mutation.engEstresse oxidativoAntioxidantesTalassemia betaBeta globinaPeróxidos lipídicosSubstância reactiva ao ácido tiobarbitúricoMutaçãoOxidative stress and antioxidant status in beta-thalassemia heterozygotesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleONDEI, Luciana de SouzaESTEVÃO, Isabeth da FonsecaROCHA, Marina Ibelli PereiraPERCÁRIO, SandroSOUZA, Dorotéia Rossi SilvaPINHEL, Marcela Augusta de SouzaDOMINGOS, Claudia Regina Boniniinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPAinstname:Universidade Federal do Pará (UFPA)instacron:UFPAORIGINALArtigo_OxidativeStressAntioxidant.pdfArtigo_OxidativeStressAntioxidant.pdfapplication/pdf248292http://repositorio.ufpa.br/oai/bitstream/2011/6126/1/Artigo_OxidativeStressAntioxidant.pdf93a1dbc90cce5e146a48736703147ad7MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.ufpa.br/oai/bitstream/2011/6126/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-822302http://repositorio.ufpa.br/oai/bitstream/2011/6126/3/license_text23f63091ab682deaf673f6bd687eadb1MD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-822190http://repositorio.ufpa.br/oai/bitstream/2011/6126/4/license_rdf19e8a2b57ef43c09f4d7071d2153c97dMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81704http://repositorio.ufpa.br/oai/bitstream/2011/6126/5/license.txt8d73ce52af4613a96b33573d09bea8c9MD55TEXTArtigo_OxidativeStressAntioxidant.pdf.txtArtigo_OxidativeStressAntioxidant.pdf.txtExtracted texttext/plain25251http://repositorio.ufpa.br/oai/bitstream/2011/6126/6/Artigo_OxidativeStressAntioxidant.pdf.txt40d4cc9a539bfb9b505be5e4f17dfcdfMD562011/61262017-10-16 12:20:52.253oai:repositorio.ufpa.br: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ório InstitucionalPUBhttp://repositorio.ufpa.br/oai/requestriufpabc@ufpa.bropendoar:21232017-10-16T15:20:52Repositório Institucional da UFPA - Universidade Federal do Pará (UFPA)false |
dc.title.pt_BR.fl_str_mv |
Oxidative stress and antioxidant status in beta-thalassemia heterozygotes |
title |
Oxidative stress and antioxidant status in beta-thalassemia heterozygotes |
spellingShingle |
Oxidative stress and antioxidant status in beta-thalassemia heterozygotes ONDEI, Luciana de Souza Estresse oxidativo Antioxidantes Talassemia beta Beta globina Peróxidos lipídicos Substância reactiva ao ácido tiobarbitúrico Mutação |
title_short |
Oxidative stress and antioxidant status in beta-thalassemia heterozygotes |
title_full |
Oxidative stress and antioxidant status in beta-thalassemia heterozygotes |
title_fullStr |
Oxidative stress and antioxidant status in beta-thalassemia heterozygotes |
title_full_unstemmed |
Oxidative stress and antioxidant status in beta-thalassemia heterozygotes |
title_sort |
Oxidative stress and antioxidant status in beta-thalassemia heterozygotes |
author |
ONDEI, Luciana de Souza |
author_facet |
ONDEI, Luciana de Souza ESTEVÃO, Isabeth da Fonseca ROCHA, Marina Ibelli Pereira PERCÁRIO, Sandro SOUZA, Dorotéia Rossi Silva PINHEL, Marcela Augusta de Souza DOMINGOS, Claudia Regina Bonini |
author_role |
author |
author2 |
ESTEVÃO, Isabeth da Fonseca ROCHA, Marina Ibelli Pereira PERCÁRIO, Sandro SOUZA, Dorotéia Rossi Silva PINHEL, Marcela Augusta de Souza DOMINGOS, Claudia Regina Bonini |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
ONDEI, Luciana de Souza ESTEVÃO, Isabeth da Fonseca ROCHA, Marina Ibelli Pereira PERCÁRIO, Sandro SOUZA, Dorotéia Rossi Silva PINHEL, Marcela Augusta de Souza DOMINGOS, Claudia Regina Bonini |
dc.subject.por.fl_str_mv |
Estresse oxidativo Antioxidantes Talassemia beta Beta globina Peróxidos lipídicos Substância reactiva ao ácido tiobarbitúrico Mutação |
topic |
Estresse oxidativo Antioxidantes Talassemia beta Beta globina Peróxidos lipídicos Substância reactiva ao ácido tiobarbitúrico Mutação |
description |
Background: Several studies have evaluated the oxidant and antioxidant status of thalassemia patients but most focused mainly on the severe and intermediate states of the disease. Moreover, the oxidative status has not been evaluated for the different beta-thalassemia mutations. Objective: To evaluate lipid peroxidation and Trolox equivalent antioxidant capacity in relation to serum iron and ferritin in beta thalassemia resulting from two different mutations (CD39 and IVS-I-110) compared to individuals without beta-thalassemia. Methods: One hundred and thirty subjects were studied, including 49 who were heterozygous for beta-thalassemia and 81 controls. Blood samples were subjected to screening tests for hemoglobin. Allele-specific polymerase chain reaction was used to confirm mutations for beta-thalassemia, an analysis of thiobarbituric acid reactive species was used to determine lipid peroxidation, and Trolox equivalent antioxidant capacity evaluations were performed. The heterozygous beta-thalassemia group was also evaluated for serum iron and ferritin status. Results: Thiobarbituric acid reactive species (486.24 ± 119.64 ng/mL) and Trolox equivalent antioxidant capacity values (2.23 ± 0.11 mM/L) were higher in beta-thalassemia heterozygotes compared to controls (260.86 ± 92.40 ng/mL and 2.12 ± 0.10 mM/L, respectively; p-value < 0.01). Increased thiobarbituric acid reactive species values were observed in subjects with the CD39 mutation compared with those with the IVS-I-110 mutation (529.94 ± 115.60 ng/mL and 453.39 ± 121.10 ng/mL, respectively; p-value = 0.04). However, average Trolox equivalent antioxidant capacity values were similar for both mutations (2.20 ± 0.08 mM/L and 2.23 ± 0.12 mM/L, respectively; p-value = 0.39). There was no influence of serum iron and ferritin levels on thiobarbituric acid reactive species and Trolox equivalent antioxidant capacity values. Conclusion: This study shows an increase of oxidative stress and antioxidant capacity in beta-thalassemia heterozygotes, mainly in carriers of the CD39 mutation. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-12 |
dc.date.accessioned.fl_str_mv |
2014-11-28T13:02:41Z |
dc.date.available.fl_str_mv |
2014-11-28T13:02:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ONDEI, Luciana de Souza et al. Oxidative stress and antioxidant status in beta-thalassemia heterozygotes. Revista Brasileira de Hematologia e Hemoterapia, São José do Rio Preto, v. 35, n. 6, p. 409-413, nov./dez. 2013. Disponível em: <http://www.scielo.br/pdf/rbhh/v35n6/1516-8484-rbhh-35-06-0409.pdf>. Acesso em: 27 nov. 2014. <http://dx.doi.org/10.5581/1516-8484.20130122>. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpa.br/jspui/handle/2011/6126 |
dc.identifier.issn.none.fl_str_mv |
1516-8484 |
identifier_str_mv |
ONDEI, Luciana de Souza et al. Oxidative stress and antioxidant status in beta-thalassemia heterozygotes. Revista Brasileira de Hematologia e Hemoterapia, São José do Rio Preto, v. 35, n. 6, p. 409-413, nov./dez. 2013. Disponível em: <http://www.scielo.br/pdf/rbhh/v35n6/1516-8484-rbhh-35-06-0409.pdf>. Acesso em: 27 nov. 2014. <http://dx.doi.org/10.5581/1516-8484.20130122>. 1516-8484 |
url |
http://repositorio.ufpa.br/jspui/handle/2011/6126 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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