Avaliação de seis testes sorológicos no diagnóstico da brucelose bubalina

Detalhes bibliográficos
Autor(a) principal: MOLNÁR, László
Data de Publicação: 2002
Outros Autores: MOLNÁR, Lászlóné Éva, NUNES, Emilia do Socorro Conceição de Lima, DIAS, Hilma Lúcia Tavares
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFPA
Texto Completo: http://repositorio.ufpa.br/jspui/handle/2011/4110
Resumo: Four hundred and forty buffalo sera, selected from about 1,200 blood samples of another study, were examined. The samples were tested by six serological methods: two of agglutination, two of indirect ELISA and two of competitive ELISA. To determine the relative sensitivity and specificity of different tests, animals with a positive result to competitive ELISA of the FAO/IAEA were considered as infected. The relative sensitivity of competitive ELISA, indirect ELISA with conjugate anti-bovine light chain monoclonal antibody labelled with HRPO, indirect ELISA with anti-bovine IgG conjugate, rose Bengal test and rapid slide agglutination test was 100%, 98.57%, 97.14%, 91.42% and 79.28%, and the relative specificity 99.33%, 97.33%, 95.66%, 94.00% and 86.33%, respectively. The value of the different serological tests for the diagnosis of brucellosis is discussed.
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spelling 2013-08-12T14:19:52Z2013-08-12T14:19:52Z2002-04MOLNÁR, László, et al. Avaliação de seis testes sorológicos no diagnóstico da brucelose bubalina. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 22, n. 2, p. 41-44, abr. 2002. Disponível em: <http://www.scielo.br/pdf/pvb/v22n2/10691.pdf>. Acesso em: 30 jul. 2013. <http://dx.doi.org/10.1590/S0100-736X2002000200002>.1678-51500100-736Xhttp://repositorio.ufpa.br/jspui/handle/2011/4110Four hundred and forty buffalo sera, selected from about 1,200 blood samples of another study, were examined. The samples were tested by six serological methods: two of agglutination, two of indirect ELISA and two of competitive ELISA. To determine the relative sensitivity and specificity of different tests, animals with a positive result to competitive ELISA of the FAO/IAEA were considered as infected. The relative sensitivity of competitive ELISA, indirect ELISA with conjugate anti-bovine light chain monoclonal antibody labelled with HRPO, indirect ELISA with anti-bovine IgG conjugate, rose Bengal test and rapid slide agglutination test was 100%, 98.57%, 97.14%, 91.42% and 79.28%, and the relative specificity 99.33%, 97.33%, 95.66%, 94.00% and 86.33%, respectively. 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dc.title.pt_BR.fl_str_mv Avaliação de seis testes sorológicos no diagnóstico da brucelose bubalina
title Avaliação de seis testes sorológicos no diagnóstico da brucelose bubalina
spellingShingle Avaliação de seis testes sorológicos no diagnóstico da brucelose bubalina
MOLNÁR, László
Brucelose em animais
Bubalinos
Ensaio de imunoadsorção enzimática
Aglutinação
Reação de Imunofluorescência Indireta (RIFI)
title_short Avaliação de seis testes sorológicos no diagnóstico da brucelose bubalina
title_full Avaliação de seis testes sorológicos no diagnóstico da brucelose bubalina
title_fullStr Avaliação de seis testes sorológicos no diagnóstico da brucelose bubalina
title_full_unstemmed Avaliação de seis testes sorológicos no diagnóstico da brucelose bubalina
title_sort Avaliação de seis testes sorológicos no diagnóstico da brucelose bubalina
author MOLNÁR, László
author_facet MOLNÁR, László
MOLNÁR, Lászlóné Éva
NUNES, Emilia do Socorro Conceição de Lima
DIAS, Hilma Lúcia Tavares
author_role author
author2 MOLNÁR, Lászlóné Éva
NUNES, Emilia do Socorro Conceição de Lima
DIAS, Hilma Lúcia Tavares
author2_role author
author
author
dc.contributor.author.fl_str_mv MOLNÁR, László
MOLNÁR, Lászlóné Éva
NUNES, Emilia do Socorro Conceição de Lima
DIAS, Hilma Lúcia Tavares
dc.subject.por.fl_str_mv Brucelose em animais
Bubalinos
Ensaio de imunoadsorção enzimática
Aglutinação
Reação de Imunofluorescência Indireta (RIFI)
topic Brucelose em animais
Bubalinos
Ensaio de imunoadsorção enzimática
Aglutinação
Reação de Imunofluorescência Indireta (RIFI)
description Four hundred and forty buffalo sera, selected from about 1,200 blood samples of another study, were examined. The samples were tested by six serological methods: two of agglutination, two of indirect ELISA and two of competitive ELISA. To determine the relative sensitivity and specificity of different tests, animals with a positive result to competitive ELISA of the FAO/IAEA were considered as infected. The relative sensitivity of competitive ELISA, indirect ELISA with conjugate anti-bovine light chain monoclonal antibody labelled with HRPO, indirect ELISA with anti-bovine IgG conjugate, rose Bengal test and rapid slide agglutination test was 100%, 98.57%, 97.14%, 91.42% and 79.28%, and the relative specificity 99.33%, 97.33%, 95.66%, 94.00% and 86.33%, respectively. The value of the different serological tests for the diagnosis of brucellosis is discussed.
publishDate 2002
dc.date.issued.fl_str_mv 2002-04
dc.date.accessioned.fl_str_mv 2013-08-12T14:19:52Z
dc.date.available.fl_str_mv 2013-08-12T14:19:52Z
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dc.identifier.citation.fl_str_mv MOLNÁR, László, et al. Avaliação de seis testes sorológicos no diagnóstico da brucelose bubalina. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 22, n. 2, p. 41-44, abr. 2002. Disponível em: <http://www.scielo.br/pdf/pvb/v22n2/10691.pdf>. Acesso em: 30 jul. 2013. <http://dx.doi.org/10.1590/S0100-736X2002000200002>.
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0100-736X
identifier_str_mv MOLNÁR, László, et al. Avaliação de seis testes sorológicos no diagnóstico da brucelose bubalina. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 22, n. 2, p. 41-44, abr. 2002. Disponível em: <http://www.scielo.br/pdf/pvb/v22n2/10691.pdf>. Acesso em: 30 jul. 2013. <http://dx.doi.org/10.1590/S0100-736X2002000200002>.
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