Genetic differentiation in populations of Aedes aegypti (Diptera, Culicidae) dengue vector from the Brazilian state of Maranhão

• Autor(a) principal: SOUSA, Andrelina Alves de
• Data de Publicação: 2017
• Outros Autores: FRAGA, Elmary da Costa, SAMPAIO, Maria Iracilda da Cunha, SCHNEIDER, Horacio, BARROS, Maria Claudene
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UFPA
• Texto Completo: http://repositorio.ufpa.br/jspui/handle/2011/9542; http://dx.doi.org/10.1016/j.rbe.2016.10.003
• topic: CNPQ::CIENCIAS BIOLOGICAS::GENETICA; Fluxo de genes; DNA mitocondrial; ND4 mitocondrial; Aedes aegypti - Diferenciação genética (MA); Zika vírus; Febre amarela; Dengue; Chikungunya

Aedes (Stegomyia) aegypti is the vector responsible for the transmission of the viruses that cause zika, yellow and chikungunya fevers, the four dengue fever serotypes (DENV - 1, 2, 3, 4), and hemorrhagic dengue fever in tropical and subtropical regions around the world. The present study investigated the genetic differentiation of the 15 populations of this vector in the Brazilian state of Maranhão, based on the mitochondrial ND4 marker. A total of 177 sequences were obtained for Aedes aegypti, with a fragment of 337 bps, 15 haplotypes, 15 polymorphics sites, haplotype diversity of h = 0.6938, and nucleotide diversity of π = 0.01486. The neutrality tests (D and Fs) were not significant. The AMOVA revealed that most of the variation (58.47%) was found within populations, with FST = 0.41533 (p < 0.05). Possible isolation by distance was tested and a significant correlation coefficient (r = 0.3486; p = 0.0040) was found using the Mantel test. The phylogenetic relationships among the 15 haplotypes indicated the existence of two distinct clades. This finding, together with the population parameters, was consistent with a pattern of genetic structuring that underpinned the genetic differentiation of the study populations in Maranhão, and was characterized by the presence of distinct lineages of Aedes aegypti.