The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of rats
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFPA |
Texto Completo: | http://repositorio.ufpa.br/jspui/2011/2486 |
Resumo: | In order to determine the modulation of anxiolytic and panicolytic-like effects of diazepam by the hormonal cycle of female rats, male and female rats – the latter divided per estrous cycle phase (estrus, diestrus, metaestrus and proestrus) – were tested in the elevated T-maze, a behavioral model of panic and anxiety. Diazepam (0.5, 1.0 and 2.0 mg/kg) or saline solution was injected in individual animals that were submitted to one session in the elevated T-maze 25 min after drug/saline administration. The test consisted of three avoidance trials and one escape trial, separated by a 30 s interval, during which the animals were isolated in individual cages. The avoidance trials began with the animal being placed at the end of the maze's enclosed arm. The time necessary for the animal to leave the central square was considered as the response's latency. The trials that exceeded 300 s were considered as failures. Results demonstrate a decrease in the effects of diazepam in inhibitory avoidance (anxiety) trials in females in diestrus and proestrus, but no relation of gender or estrous cycle on diazepam effects on escape trials (fear). The results support the hypothesis that down-regulation of GABAA receptors by activation of nuclear estrogen receptors and induction of PKC-mediated GABAA receptor phosphorylation by activation of surface estrogen receptors in raphe neurons underlie the modulation of diazepam sensitivity by estrogen. |
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2012-03-01T15:10:11Z2012-03-01T15:10:11Z2009-12GOUVEIA JR, Amauri et al. The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of rats. Psychology & Neuroscience, Rio de Janeiro, v. 2, n. 2, p. 227-233, dez. 2009. Disponível em: <http://www.scielo.br/pdf/pn/v2n2/a16v2n2.pdf>. Acesso em: 29 fev. 2011. http://dx.doi.org/10.3922/j.psns.2009.2.016.1983-3288http://repositorio.ufpa.br/jspui/2011/2486In order to determine the modulation of anxiolytic and panicolytic-like effects of diazepam by the hormonal cycle of female rats, male and female rats – the latter divided per estrous cycle phase (estrus, diestrus, metaestrus and proestrus) – were tested in the elevated T-maze, a behavioral model of panic and anxiety. Diazepam (0.5, 1.0 and 2.0 mg/kg) or saline solution was injected in individual animals that were submitted to one session in the elevated T-maze 25 min after drug/saline administration. The test consisted of three avoidance trials and one escape trial, separated by a 30 s interval, during which the animals were isolated in individual cages. The avoidance trials began with the animal being placed at the end of the maze's enclosed arm. The time necessary for the animal to leave the central square was considered as the response's latency. The trials that exceeded 300 s were considered as failures. Results demonstrate a decrease in the effects of diazepam in inhibitory avoidance (anxiety) trials in females in diestrus and proestrus, but no relation of gender or estrous cycle on diazepam effects on escape trials (fear). The results support the hypothesis that down-regulation of GABAA receptors by activation of nuclear estrogen receptors and induction of PKC-mediated GABAA receptor phosphorylation by activation of surface estrogen receptors in raphe neurons underlie the modulation of diazepam sensitivity by estrogen.engAnsiedadeComportamento animalFarmacologia experimentalEstímulo/respostaRoedorThe effects of diazepam on the elevated T-maze are dependent on the estrous cycle of ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGOUVEIA JUNIOR, AmauriANTUNES, GabrielaOLIVEIRA, Caio Maximino deCARVALHO, Silvio Morato deinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPAinstname:Universidade Federal do Pará (UFPA)instacron:UFPAORIGINALArtigo_EffectsDiazepamElevated.pdfArtigo_EffectsDiazepamElevated.pdfapplication/pdf293829http://repositorio.ufpa.br/oai/bitstream/2011/2486/1/Artigo_EffectsDiazepamElevated.pdfe57f9e696f08655ee8df3b21eee3a034MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-823599http://repositorio.ufpa.br/oai/bitstream/2011/2486/2/license_rdf9e2b7f6edbd693264102b96ece20428aMD52license_textlicense_texttext/html; charset=utf-80http://repositorio.ufpa.br/oai/bitstream/2011/2486/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_urllicense_urltext/plain; charset=utf-849http://repositorio.ufpa.br/oai/bitstream/2011/2486/4/license_urlfd26723f8d7edacdb29e3f03465c3b03MD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.ufpa.br/oai/bitstream/2011/2486/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD55TEXTArtigo_EffectsDiazepamElevated.pdf.txtArtigo_EffectsDiazepamElevated.pdf.txtExtracted texttext/plain38811http://repositorio.ufpa.br/oai/bitstream/2011/2486/6/Artigo_EffectsDiazepamElevated.pdf.txtfb2b7b944a34687f7d6c00e22bf410f8MD562011/24862017-10-16 11:29:55.392oai:repositorio.ufpa.br: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Repositório InstitucionalPUBhttp://repositorio.ufpa.br/oai/requestriufpabc@ufpa.bropendoar:21232017-10-16T14:29:55Repositório Institucional da UFPA - Universidade Federal do Pará (UFPA)false |
dc.title.pt_BR.fl_str_mv |
The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of rats |
title |
The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of rats |
spellingShingle |
The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of rats GOUVEIA JUNIOR, Amauri Ansiedade Comportamento animal Farmacologia experimental Estímulo/resposta Roedor |
title_short |
The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of rats |
title_full |
The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of rats |
title_fullStr |
The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of rats |
title_full_unstemmed |
The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of rats |
title_sort |
The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of rats |
author |
GOUVEIA JUNIOR, Amauri |
author_facet |
GOUVEIA JUNIOR, Amauri ANTUNES, Gabriela OLIVEIRA, Caio Maximino de CARVALHO, Silvio Morato de |
author_role |
author |
author2 |
ANTUNES, Gabriela OLIVEIRA, Caio Maximino de CARVALHO, Silvio Morato de |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
GOUVEIA JUNIOR, Amauri ANTUNES, Gabriela OLIVEIRA, Caio Maximino de CARVALHO, Silvio Morato de |
dc.subject.por.fl_str_mv |
Ansiedade Comportamento animal Farmacologia experimental Estímulo/resposta Roedor |
topic |
Ansiedade Comportamento animal Farmacologia experimental Estímulo/resposta Roedor |
description |
In order to determine the modulation of anxiolytic and panicolytic-like effects of diazepam by the hormonal cycle of female rats, male and female rats – the latter divided per estrous cycle phase (estrus, diestrus, metaestrus and proestrus) – were tested in the elevated T-maze, a behavioral model of panic and anxiety. Diazepam (0.5, 1.0 and 2.0 mg/kg) or saline solution was injected in individual animals that were submitted to one session in the elevated T-maze 25 min after drug/saline administration. The test consisted of three avoidance trials and one escape trial, separated by a 30 s interval, during which the animals were isolated in individual cages. The avoidance trials began with the animal being placed at the end of the maze's enclosed arm. The time necessary for the animal to leave the central square was considered as the response's latency. The trials that exceeded 300 s were considered as failures. Results demonstrate a decrease in the effects of diazepam in inhibitory avoidance (anxiety) trials in females in diestrus and proestrus, but no relation of gender or estrous cycle on diazepam effects on escape trials (fear). The results support the hypothesis that down-regulation of GABAA receptors by activation of nuclear estrogen receptors and induction of PKC-mediated GABAA receptor phosphorylation by activation of surface estrogen receptors in raphe neurons underlie the modulation of diazepam sensitivity by estrogen. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009-12 |
dc.date.accessioned.fl_str_mv |
2012-03-01T15:10:11Z |
dc.date.available.fl_str_mv |
2012-03-01T15:10:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
GOUVEIA JR, Amauri et al. The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of rats. Psychology & Neuroscience, Rio de Janeiro, v. 2, n. 2, p. 227-233, dez. 2009. Disponível em: <http://www.scielo.br/pdf/pn/v2n2/a16v2n2.pdf>. Acesso em: 29 fev. 2011. http://dx.doi.org/10.3922/j.psns.2009.2.016. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpa.br/jspui/2011/2486 |
dc.identifier.issn.none.fl_str_mv |
1983-3288 |
identifier_str_mv |
GOUVEIA JR, Amauri et al. The effects of diazepam on the elevated T-maze are dependent on the estrous cycle of rats. Psychology & Neuroscience, Rio de Janeiro, v. 2, n. 2, p. 227-233, dez. 2009. Disponível em: <http://www.scielo.br/pdf/pn/v2n2/a16v2n2.pdf>. Acesso em: 29 fev. 2011. http://dx.doi.org/10.3922/j.psns.2009.2.016. 1983-3288 |
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http://repositorio.ufpa.br/jspui/2011/2486 |
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eng |
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eng |
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