Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFPA |
Texto Completo: | http://repositorio.ufpa.br/jspui/handle/2011/5178 |
Resumo: | Gastric cancer is the fourth most frequent type of cancer and the second cause of cancer mortality worldwide. The genetic alterations described so far for gastric carcinomas include amplifications and mutations of the c-ERBB2, KRAS, MET, TP53, and c-MYC genes. Chromosomal instability described for gastric cancer includes gains and losses of whole chromosomes or parts of them and these events might lead to oncogene overexpression, showing the need for a better understanding of the cytogenetic aspects of this neoplasia. Very few gastric carcinoma cell lines have been isolated. The establishment and characterization of the biological properties of gastric cancer cell lines is a powerful tool to gather information about the evolution of this malignancy, and also to test new therapeutic approaches. The present study characterized cytogenetically PG100, the first commercially available gastric cancer cell line derived from a Brazilian patient who had a gastric adenocarcinoma, using GTG banding and fluorescent in situ hybridization to determine MYC amplification. Twenty metaphases were karyotyped; 19 (95%) of them presented chromosome 8 trisomy, where the MYC gene is located, and 17 (85%) presented a deletion in the 17p region, where the TP53 is located. These are common findings for gastric carcinomas, validating PG100 as an experimental model for this neoplasia. Eighty-six percent of 200 cells analyzed by fluorescent in situ hybridization presented MYC overexpression. Less frequent findings, such as 5p deletions and trisomy 16, open new perspectives for the study of this tumor. |
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2014-06-30T12:47:03Z2014-06-30T12:47:03Z2010-08RIBEIRO, H.F. et al. Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 43, n. 8, p. 717-721, ago. 2010. Disponível em: <http://www.scielo.br/pdf/bjmbr/v43n8/307.pdf>. Acesso em: 24 fev. 2014. <http://dx.doi.org/10.1590/S0100-879X2010007500068>.1414-431Xhttp://repositorio.ufpa.br/jspui/handle/2011/5178Gastric cancer is the fourth most frequent type of cancer and the second cause of cancer mortality worldwide. The genetic alterations described so far for gastric carcinomas include amplifications and mutations of the c-ERBB2, KRAS, MET, TP53, and c-MYC genes. Chromosomal instability described for gastric cancer includes gains and losses of whole chromosomes or parts of them and these events might lead to oncogene overexpression, showing the need for a better understanding of the cytogenetic aspects of this neoplasia. Very few gastric carcinoma cell lines have been isolated. The establishment and characterization of the biological properties of gastric cancer cell lines is a powerful tool to gather information about the evolution of this malignancy, and also to test new therapeutic approaches. The present study characterized cytogenetically PG100, the first commercially available gastric cancer cell line derived from a Brazilian patient who had a gastric adenocarcinoma, using GTG banding and fluorescent in situ hybridization to determine MYC amplification. Twenty metaphases were karyotyped; 19 (95%) of them presented chromosome 8 trisomy, where the MYC gene is located, and 17 (85%) presented a deletion in the 17p region, where the TP53 is located. These are common findings for gastric carcinomas, validating PG100 as an experimental model for this neoplasia. Eighty-six percent of 200 cells analyzed by fluorescent in situ hybridization presented MYC overexpression. Less frequent findings, such as 5p deletions and trisomy 16, open new perspectives for the study of this tumor.engCarcinogêneseCromossomos humanosCâncer gástricoOncogenesAnormalidades cromossômicasCarcinoma gástricoAlterações cromossômicasAlterações genéticasCytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell lineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRIBEIRO, Helem FerreiraALCÂNTARA, Diego Di Felipe ÁvilaMATOS, Leomá AlbuquerqueSOUSA, João Marcelo de Castro eLEAL, Mariana FerreiraSMITH, Marília de Arruda CardosoRODRÍGUEZ BURBANO, Rommel MarioBAHIA, Marcelo de Oliveirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPAinstname:Universidade Federal do Pará (UFPA)instacron:UFPAORIGINALArtigo_CytogeneticCharacterizationsEvaluation.pdfArtigo_CytogeneticCharacterizationsEvaluation.pdfapplication/pdf585334http://repositorio.ufpa.br/oai/bitstream/2011/5178/1/Artigo_CytogeneticCharacterizationsEvaluation.pdff5f9bff7a2e31da85092dd740c411454MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.ufpa.br/oai/bitstream/2011/5178/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-821936http://repositorio.ufpa.br/oai/bitstream/2011/5178/3/license_text9833653f73f7853880c94a6fead477b1MD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-823148http://repositorio.ufpa.br/oai/bitstream/2011/5178/4/license_rdf9da0b6dfac957114c6a7714714b86306MD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81775http://repositorio.ufpa.br/oai/bitstream/2011/5178/5/license.txta930293e49ae6e6b5c49a341d4a36286MD55TEXTArtigo_CytogeneticCharacterizationsEvaluation.pdf.txtArtigo_CytogeneticCharacterizationsEvaluation.pdf.txtExtracted texttext/plain20858http://repositorio.ufpa.br/oai/bitstream/2011/5178/6/Artigo_CytogeneticCharacterizationsEvaluation.pdf.txt2257834a8ea7110c5b067598e3c85763MD562011/51782017-10-16 12:20:41.97oai:repositorio.ufpa.br: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Repositório InstitucionalPUBhttp://repositorio.ufpa.br/oai/requestriufpabc@ufpa.bropendoar:21232017-10-16T15:20:41Repositório Institucional da UFPA - Universidade Federal do Pará (UFPA)false |
dc.title.pt_BR.fl_str_mv |
Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line |
title |
Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line |
spellingShingle |
Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line RIBEIRO, Helem Ferreira Carcinogênese Cromossomos humanos Câncer gástrico Oncogenes Anormalidades cromossômicas Carcinoma gástrico Alterações cromossômicas Alterações genéticas |
title_short |
Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line |
title_full |
Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line |
title_fullStr |
Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line |
title_full_unstemmed |
Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line |
title_sort |
Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line |
author |
RIBEIRO, Helem Ferreira |
author_facet |
RIBEIRO, Helem Ferreira ALCÂNTARA, Diego Di Felipe Ávila MATOS, Leomá Albuquerque SOUSA, João Marcelo de Castro e LEAL, Mariana Ferreira SMITH, Marília de Arruda Cardoso RODRÍGUEZ BURBANO, Rommel Mario BAHIA, Marcelo de Oliveira |
author_role |
author |
author2 |
ALCÂNTARA, Diego Di Felipe Ávila MATOS, Leomá Albuquerque SOUSA, João Marcelo de Castro e LEAL, Mariana Ferreira SMITH, Marília de Arruda Cardoso RODRÍGUEZ BURBANO, Rommel Mario BAHIA, Marcelo de Oliveira |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
RIBEIRO, Helem Ferreira ALCÂNTARA, Diego Di Felipe Ávila MATOS, Leomá Albuquerque SOUSA, João Marcelo de Castro e LEAL, Mariana Ferreira SMITH, Marília de Arruda Cardoso RODRÍGUEZ BURBANO, Rommel Mario BAHIA, Marcelo de Oliveira |
dc.subject.por.fl_str_mv |
Carcinogênese Cromossomos humanos Câncer gástrico Oncogenes Anormalidades cromossômicas Carcinoma gástrico Alterações cromossômicas Alterações genéticas |
topic |
Carcinogênese Cromossomos humanos Câncer gástrico Oncogenes Anormalidades cromossômicas Carcinoma gástrico Alterações cromossômicas Alterações genéticas |
description |
Gastric cancer is the fourth most frequent type of cancer and the second cause of cancer mortality worldwide. The genetic alterations described so far for gastric carcinomas include amplifications and mutations of the c-ERBB2, KRAS, MET, TP53, and c-MYC genes. Chromosomal instability described for gastric cancer includes gains and losses of whole chromosomes or parts of them and these events might lead to oncogene overexpression, showing the need for a better understanding of the cytogenetic aspects of this neoplasia. Very few gastric carcinoma cell lines have been isolated. The establishment and characterization of the biological properties of gastric cancer cell lines is a powerful tool to gather information about the evolution of this malignancy, and also to test new therapeutic approaches. The present study characterized cytogenetically PG100, the first commercially available gastric cancer cell line derived from a Brazilian patient who had a gastric adenocarcinoma, using GTG banding and fluorescent in situ hybridization to determine MYC amplification. Twenty metaphases were karyotyped; 19 (95%) of them presented chromosome 8 trisomy, where the MYC gene is located, and 17 (85%) presented a deletion in the 17p region, where the TP53 is located. These are common findings for gastric carcinomas, validating PG100 as an experimental model for this neoplasia. Eighty-six percent of 200 cells analyzed by fluorescent in situ hybridization presented MYC overexpression. Less frequent findings, such as 5p deletions and trisomy 16, open new perspectives for the study of this tumor. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-08 |
dc.date.accessioned.fl_str_mv |
2014-06-30T12:47:03Z |
dc.date.available.fl_str_mv |
2014-06-30T12:47:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RIBEIRO, H.F. et al. Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 43, n. 8, p. 717-721, ago. 2010. Disponível em: <http://www.scielo.br/pdf/bjmbr/v43n8/307.pdf>. Acesso em: 24 fev. 2014. <http://dx.doi.org/10.1590/S0100-879X2010007500068>. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpa.br/jspui/handle/2011/5178 |
dc.identifier.issn.none.fl_str_mv |
1414-431X |
identifier_str_mv |
RIBEIRO, H.F. et al. Cytogenetic characterization and evaluation of c-MYC gene amplification in PG100, a new Brazilian gastric cancer cell line. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 43, n. 8, p. 717-721, ago. 2010. Disponível em: <http://www.scielo.br/pdf/bjmbr/v43n8/307.pdf>. Acesso em: 24 fev. 2014. <http://dx.doi.org/10.1590/S0100-879X2010007500068>. 1414-431X |
url |
http://repositorio.ufpa.br/jspui/handle/2011/5178 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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