Relationships between dendritic morphology, spatial distribution and firing patterns in rat layer 1 neurons
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFPA |
Texto Completo: | http://repositorio.ufpa.br/jspui/handle/2011/5150 |
Resumo: | The cortical layer 1 contains mainly small interneurons, which have traditionally been classified according to their axonal morphology. The dendritic morphology of these cells, however, has received little attention and remains ill defined. Very little is known about how the dendritic morphology and spatial distribution of these cells may relate to functional neuronal properties. We used biocytin labeling and whole cell patch clamp recordings, associated with digital reconstruction and quantitative morphological analysis, to assess correlations between dendritic morphology, spatial distribution and membrane properties of rat layer 1 neurons. A total of 106 cells were recorded, labeled and subjected to morphological analysis. Based on the quantitative patterns of their dendritic arbor, cells were divided into four major morphotypes: horizontal, radial, ascendant, and descendant cells. Descendant cells exhibited a highly distinct spatial distribution in relation to other morphotypes, suggesting that they may have a distinct function in these cortical circuits. A significant difference was also found in the distribution of firing patterns between each morphotype and between the neuronal populations of each sublayer. Passive membrane properties were, however, statistically homogeneous among all subgroups. We speculate that the differences observed in active membrane properties might be related to differences in the synaptic input of specific types of afferent fibers and to differences in the computational roles of each morphotype in layer 1 circuits. Our findings provide new insights into dendritic morphology and neuronal spatial distribution in layer 1 circuits, indicating that variations in these properties may be correlated with distinct physiological functions. |
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2014-06-26T13:06:56Z2014-06-26T13:06:56Z2012-12SANTOS, D.V.V. et al. Relationships between dendritic morphology, spatial distribution and firing patterns in rat layer 1 neurons. Brazilian Journal of Medical and Biological Research, v. 45, n. 12, p. 1221-1233, dez. 2012. Disponível em: <http://www.scielo.br/pdf/bjmbr/v45n12/1957.pdf>. Acesso em: 20 fev. 2014.0100-879Xhttp://repositorio.ufpa.br/jspui/handle/2011/5150The cortical layer 1 contains mainly small interneurons, which have traditionally been classified according to their axonal morphology. The dendritic morphology of these cells, however, has received little attention and remains ill defined. Very little is known about how the dendritic morphology and spatial distribution of these cells may relate to functional neuronal properties. We used biocytin labeling and whole cell patch clamp recordings, associated with digital reconstruction and quantitative morphological analysis, to assess correlations between dendritic morphology, spatial distribution and membrane properties of rat layer 1 neurons. A total of 106 cells were recorded, labeled and subjected to morphological analysis. Based on the quantitative patterns of their dendritic arbor, cells were divided into four major morphotypes: horizontal, radial, ascendant, and descendant cells. Descendant cells exhibited a highly distinct spatial distribution in relation to other morphotypes, suggesting that they may have a distinct function in these cortical circuits. A significant difference was also found in the distribution of firing patterns between each morphotype and between the neuronal populations of each sublayer. Passive membrane properties were, however, statistically homogeneous among all subgroups. We speculate that the differences observed in active membrane properties might be related to differences in the synaptic input of specific types of afferent fibers and to differences in the computational roles of each morphotype in layer 1 circuits. Our findings provide new insights into dendritic morphology and neuronal spatial distribution in layer 1 circuits, indicating that variations in these properties may be correlated with distinct physiological functions.engBiocitinaPatch-clampMorfologia dentríticaCamada Cortical 1Variabilidade neuronalRelationships between dendritic morphology, spatial distribution and firing patterns in rat layer 1 neuronsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSANTOS, Daniel Valle VasconcelosCOSTA, Kauê MachadoSILVA FILHO, Manoel daVAZ, Manoella Cristina Gadelhainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPAinstname:Universidade Federal do Pará (UFPA)instacron:UFPAORIGINALArtigo_RelationsshipsDendriticMorphology.pdfArtigo_RelationsshipsDendriticMorphology.pdfapplication/pdf2975507http://repositorio.ufpa.br/oai/bitstream/2011/5150/1/Artigo_RelationsshipsDendriticMorphology.pdf4d47f1c64508fd2d664de02f6b623f53MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.ufpa.br/oai/bitstream/2011/5150/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-821936http://repositorio.ufpa.br/oai/bitstream/2011/5150/3/license_text9833653f73f7853880c94a6fead477b1MD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-823148http://repositorio.ufpa.br/oai/bitstream/2011/5150/4/license_rdf9da0b6dfac957114c6a7714714b86306MD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81775http://repositorio.ufpa.br/oai/bitstream/2011/5150/5/license.txta930293e49ae6e6b5c49a341d4a36286MD55TEXTArtigo_RelationsshipsDendriticMorphology.pdf.txtArtigo_RelationsshipsDendriticMorphology.pdf.txtExtracted texttext/plain49053http://repositorio.ufpa.br/oai/bitstream/2011/5150/6/Artigo_RelationsshipsDendriticMorphology.pdf.txt5c2e2fd2dab65ed5bf2107578eb21ad9MD562011/51502017-10-16 12:20:44.446oai:repositorio.ufpa.br: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Repositório InstitucionalPUBhttp://repositorio.ufpa.br/oai/requestriufpabc@ufpa.bropendoar:21232017-10-16T15:20:44Repositório Institucional da UFPA - Universidade Federal do Pará (UFPA)false |
dc.title.pt_BR.fl_str_mv |
Relationships between dendritic morphology, spatial distribution and firing patterns in rat layer 1 neurons |
title |
Relationships between dendritic morphology, spatial distribution and firing patterns in rat layer 1 neurons |
spellingShingle |
Relationships between dendritic morphology, spatial distribution and firing patterns in rat layer 1 neurons SANTOS, Daniel Valle Vasconcelos Biocitina Patch-clamp Morfologia dentrítica Camada Cortical 1 Variabilidade neuronal |
title_short |
Relationships between dendritic morphology, spatial distribution and firing patterns in rat layer 1 neurons |
title_full |
Relationships between dendritic morphology, spatial distribution and firing patterns in rat layer 1 neurons |
title_fullStr |
Relationships between dendritic morphology, spatial distribution and firing patterns in rat layer 1 neurons |
title_full_unstemmed |
Relationships between dendritic morphology, spatial distribution and firing patterns in rat layer 1 neurons |
title_sort |
Relationships between dendritic morphology, spatial distribution and firing patterns in rat layer 1 neurons |
author |
SANTOS, Daniel Valle Vasconcelos |
author_facet |
SANTOS, Daniel Valle Vasconcelos COSTA, Kauê Machado SILVA FILHO, Manoel da VAZ, Manoella Cristina Gadelha |
author_role |
author |
author2 |
COSTA, Kauê Machado SILVA FILHO, Manoel da VAZ, Manoella Cristina Gadelha |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
SANTOS, Daniel Valle Vasconcelos COSTA, Kauê Machado SILVA FILHO, Manoel da VAZ, Manoella Cristina Gadelha |
dc.subject.por.fl_str_mv |
Biocitina Patch-clamp Morfologia dentrítica Camada Cortical 1 Variabilidade neuronal |
topic |
Biocitina Patch-clamp Morfologia dentrítica Camada Cortical 1 Variabilidade neuronal |
description |
The cortical layer 1 contains mainly small interneurons, which have traditionally been classified according to their axonal morphology. The dendritic morphology of these cells, however, has received little attention and remains ill defined. Very little is known about how the dendritic morphology and spatial distribution of these cells may relate to functional neuronal properties. We used biocytin labeling and whole cell patch clamp recordings, associated with digital reconstruction and quantitative morphological analysis, to assess correlations between dendritic morphology, spatial distribution and membrane properties of rat layer 1 neurons. A total of 106 cells were recorded, labeled and subjected to morphological analysis. Based on the quantitative patterns of their dendritic arbor, cells were divided into four major morphotypes: horizontal, radial, ascendant, and descendant cells. Descendant cells exhibited a highly distinct spatial distribution in relation to other morphotypes, suggesting that they may have a distinct function in these cortical circuits. A significant difference was also found in the distribution of firing patterns between each morphotype and between the neuronal populations of each sublayer. Passive membrane properties were, however, statistically homogeneous among all subgroups. We speculate that the differences observed in active membrane properties might be related to differences in the synaptic input of specific types of afferent fibers and to differences in the computational roles of each morphotype in layer 1 circuits. Our findings provide new insights into dendritic morphology and neuronal spatial distribution in layer 1 circuits, indicating that variations in these properties may be correlated with distinct physiological functions. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-12 |
dc.date.accessioned.fl_str_mv |
2014-06-26T13:06:56Z |
dc.date.available.fl_str_mv |
2014-06-26T13:06:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SANTOS, D.V.V. et al. Relationships between dendritic morphology, spatial distribution and firing patterns in rat layer 1 neurons. Brazilian Journal of Medical and Biological Research, v. 45, n. 12, p. 1221-1233, dez. 2012. Disponível em: <http://www.scielo.br/pdf/bjmbr/v45n12/1957.pdf>. Acesso em: 20 fev. 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpa.br/jspui/handle/2011/5150 |
dc.identifier.issn.none.fl_str_mv |
0100-879X |
identifier_str_mv |
SANTOS, D.V.V. et al. Relationships between dendritic morphology, spatial distribution and firing patterns in rat layer 1 neurons. Brazilian Journal of Medical and Biological Research, v. 45, n. 12, p. 1221-1233, dez. 2012. Disponível em: <http://www.scielo.br/pdf/bjmbr/v45n12/1957.pdf>. Acesso em: 20 fev. 2014. 0100-879X |
url |
http://repositorio.ufpa.br/jspui/handle/2011/5150 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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