Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle

Detalhes bibliográficos
Autor(a) principal: RAMOS, Carlos Alberto do Nascimento
Data de Publicação: 2014
Outros Autores: ARAÚJO, Flábio Ribeiro de, SANTOS, Rafaelle Cunha dos, MELO, Elaine Silva de Pádua, SOUSA, Letícia Carvalho de, VIDAL, Carlos Eugênio Soto, GUERRA, Neurisvan Ramos Guerra, RAMOS, Rafael Antonio do Nascimento
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFPA
Texto Completo: http://repositorio.ufpa.br/jspui/handle/2011/6589
Resumo: The recombinant protein MSP5 has been established as an important antigen for serological diagnosis of Anaplasma marginale by enzyme-linked immunosorbent assay (ELISA). However, due to the high cost of specialized equipment, this technique is not accessible to all laboratories, especially in developing countries in areas where the disease is endemic. The present study describes the standardization of a latex agglutination test (LAT) to detect antibodies against A. marginale based on recombinant MSP5. Compared with indirect enzyme-linked immunosorbent assay (iELISA), the relative sensitivity and specificity of the LAT were 95.21% and 91.86% respectively, with an almost perfect agreement between tests (kappa index = 0.863). These results can be considered important for the serological diagnosis of A. marginale, as they indicate that the test represents a rapid and low cost alternative to ELISA.
id UFPA_d2d006f9ad95f20c0cfe79314620f330
oai_identifier_str oai:repositorio.ufpa.br:2011/6589
network_acronym_str UFPA
network_name_str Repositório Institucional da UFPA
repository_id_str 2123
spelling 2015-04-28T14:31:25Z2015-04-28T14:31:25Z2014RAMOS, Carlos A.N. et al. Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle. Brazilian Journal of Microbiology, São Paulo, v. 45, n. 1, p. 199-204, 2014. Disponível em: <http://www.scielo.br/pdf/bjm/v45n1/7118.pdf>. Acesso em: 24 abr. 2015. <http://dx.doi.org/10.1590/S1517-83822014005000039>.1517-8382http://repositorio.ufpa.br/jspui/handle/2011/6589The recombinant protein MSP5 has been established as an important antigen for serological diagnosis of Anaplasma marginale by enzyme-linked immunosorbent assay (ELISA). However, due to the high cost of specialized equipment, this technique is not accessible to all laboratories, especially in developing countries in areas where the disease is endemic. The present study describes the standardization of a latex agglutination test (LAT) to detect antibodies against A. marginale based on recombinant MSP5. Compared with indirect enzyme-linked immunosorbent assay (iELISA), the relative sensitivity and specificity of the LAT were 95.21% and 91.86% respectively, with an almost perfect agreement between tests (kappa index = 0.863). These results can be considered important for the serological diagnosis of A. marginale, as they indicate that the test represents a rapid and low cost alternative to ELISA.engBovinoAnaplasma marginaleTestes de fixação do latexMSP5Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRAMOS, Carlos Alberto do NascimentoARAÚJO, Flábio Ribeiro deSANTOS, Rafaelle Cunha dosMELO, Elaine Silva de PáduaSOUSA, Letícia Carvalho deVIDAL, Carlos Eugênio SotoGUERRA, Neurisvan Ramos GuerraRAMOS, Rafael Antonio do Nascimentoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPAinstname:Universidade Federal do Pará (UFPA)instacron:UFPAORIGINALArtigo_DevelopmentAssessmentLatex.pdfArtigo_DevelopmentAssessmentLatex.pdfapplication/pdf674898http://repositorio.ufpa.br/oai/bitstream/2011/6589/1/Artigo_DevelopmentAssessmentLatex.pdf4940503d6670602657bf047b3ae861c8MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.ufpa.br/oai/bitstream/2011/6589/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-822762http://repositorio.ufpa.br/oai/bitstream/2011/6589/3/license_text321e754e6058db75d1137df814352636MD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-822190http://repositorio.ufpa.br/oai/bitstream/2011/6589/4/license_rdf19e8a2b57ef43c09f4d7071d2153c97dMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81703http://repositorio.ufpa.br/oai/bitstream/2011/6589/5/license.txta12ee01655d4f43dacf016d5e6168febMD55TEXTArtigo_DevelopmentAssessmentLatex.pdf.txtArtigo_DevelopmentAssessmentLatex.pdf.txtExtracted texttext/plain24762http://repositorio.ufpa.br/oai/bitstream/2011/6589/6/Artigo_DevelopmentAssessmentLatex.pdf.txtb720cdd0628383f36c226db60e667685MD562011/65892017-12-20 12:30:50.52oai:repositorio.ufpa.br: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Repositório InstitucionalPUBhttp://repositorio.ufpa.br/oai/requestriufpabc@ufpa.bropendoar:21232017-12-20T15:30:50Repositório Institucional da UFPA - Universidade Federal do Pará (UFPA)false
dc.title.pt_BR.fl_str_mv Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle
title Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle
spellingShingle Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle
RAMOS, Carlos Alberto do Nascimento
Bovino
Anaplasma marginale
Testes de fixação do latex
MSP5
title_short Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle
title_full Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle
title_fullStr Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle
title_full_unstemmed Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle
title_sort Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle
author RAMOS, Carlos Alberto do Nascimento
author_facet RAMOS, Carlos Alberto do Nascimento
ARAÚJO, Flábio Ribeiro de
SANTOS, Rafaelle Cunha dos
MELO, Elaine Silva de Pádua
SOUSA, Letícia Carvalho de
VIDAL, Carlos Eugênio Soto
GUERRA, Neurisvan Ramos Guerra
RAMOS, Rafael Antonio do Nascimento
author_role author
author2 ARAÚJO, Flábio Ribeiro de
SANTOS, Rafaelle Cunha dos
MELO, Elaine Silva de Pádua
SOUSA, Letícia Carvalho de
VIDAL, Carlos Eugênio Soto
GUERRA, Neurisvan Ramos Guerra
RAMOS, Rafael Antonio do Nascimento
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv RAMOS, Carlos Alberto do Nascimento
ARAÚJO, Flábio Ribeiro de
SANTOS, Rafaelle Cunha dos
MELO, Elaine Silva de Pádua
SOUSA, Letícia Carvalho de
VIDAL, Carlos Eugênio Soto
GUERRA, Neurisvan Ramos Guerra
RAMOS, Rafael Antonio do Nascimento
dc.subject.por.fl_str_mv Bovino
Anaplasma marginale
Testes de fixação do latex
MSP5
topic Bovino
Anaplasma marginale
Testes de fixação do latex
MSP5
description The recombinant protein MSP5 has been established as an important antigen for serological diagnosis of Anaplasma marginale by enzyme-linked immunosorbent assay (ELISA). However, due to the high cost of specialized equipment, this technique is not accessible to all laboratories, especially in developing countries in areas where the disease is endemic. The present study describes the standardization of a latex agglutination test (LAT) to detect antibodies against A. marginale based on recombinant MSP5. Compared with indirect enzyme-linked immunosorbent assay (iELISA), the relative sensitivity and specificity of the LAT were 95.21% and 91.86% respectively, with an almost perfect agreement between tests (kappa index = 0.863). These results can be considered important for the serological diagnosis of A. marginale, as they indicate that the test represents a rapid and low cost alternative to ELISA.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-04-28T14:31:25Z
dc.date.available.fl_str_mv 2015-04-28T14:31:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RAMOS, Carlos A.N. et al. Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle. Brazilian Journal of Microbiology, São Paulo, v. 45, n. 1, p. 199-204, 2014. Disponível em: <http://www.scielo.br/pdf/bjm/v45n1/7118.pdf>. Acesso em: 24 abr. 2015. <http://dx.doi.org/10.1590/S1517-83822014005000039>.
dc.identifier.uri.fl_str_mv http://repositorio.ufpa.br/jspui/handle/2011/6589
dc.identifier.issn.none.fl_str_mv 1517-8382
identifier_str_mv RAMOS, Carlos A.N. et al. Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle. Brazilian Journal of Microbiology, São Paulo, v. 45, n. 1, p. 199-204, 2014. Disponível em: <http://www.scielo.br/pdf/bjm/v45n1/7118.pdf>. Acesso em: 24 abr. 2015. <http://dx.doi.org/10.1590/S1517-83822014005000039>.
1517-8382
url http://repositorio.ufpa.br/jspui/handle/2011/6589
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFPA
instname:Universidade Federal do Pará (UFPA)
instacron:UFPA
instname_str Universidade Federal do Pará (UFPA)
instacron_str UFPA
institution UFPA
reponame_str Repositório Institucional da UFPA
collection Repositório Institucional da UFPA
bitstream.url.fl_str_mv http://repositorio.ufpa.br/oai/bitstream/2011/6589/1/Artigo_DevelopmentAssessmentLatex.pdf
http://repositorio.ufpa.br/oai/bitstream/2011/6589/2/license_url
http://repositorio.ufpa.br/oai/bitstream/2011/6589/3/license_text
http://repositorio.ufpa.br/oai/bitstream/2011/6589/4/license_rdf
http://repositorio.ufpa.br/oai/bitstream/2011/6589/5/license.txt
http://repositorio.ufpa.br/oai/bitstream/2011/6589/6/Artigo_DevelopmentAssessmentLatex.pdf.txt
bitstream.checksum.fl_str_mv 4940503d6670602657bf047b3ae861c8
4afdbb8c545fd630ea7db775da747b2f
321e754e6058db75d1137df814352636
19e8a2b57ef43c09f4d7071d2153c97d
a12ee01655d4f43dacf016d5e6168feb
b720cdd0628383f36c226db60e667685
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFPA - Universidade Federal do Pará (UFPA)
repository.mail.fl_str_mv riufpabc@ufpa.br
_version_ 1801771939694379008