Primary endemic Cryptococcosis gattii by molecular type VGII in the state of Pará, Brazil

• Autor(a) principal: SANTOS, Wallace Raimundo Araujo dos
• Data de Publicação: 2008
• Outros Autores: MEYER, Wieland, COSTA, Solange do Perpétuo Socorro Evangelista, TRILLES, Luciana, NASCIMENTO, José Luiz Martins do, SOUSA, Rita Catarina Medeiros, MORALES, Bernardina Penarrieta, BEZERRA, Cláudia de Carvalho Falci, MACÊDO, Regina Célia Lima de, FERREIRA, Silvana O., BARBOSA, Gláucia Gonçalves, PEREZ, Mauricio de Andrade, NISHIKAWA, Marília Martins, LAZÉRA, Márcia dos Santos
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFPA
• Texto Completo: http://www.repositorio.ufpa.br:8080/jspui/handle/2011/2363
• topic: Cryptococcus gattii; VGII; Micose; Crianças; Pará - Estado; Amazônia brasileira; Doenças endêmicas

In order to study the infectious agents causing human disseminated cryptococcosis in the state of Pará, North Brazil, 56 isolates of Cryptococcusspp. (54 isolated from cerebral spinal fluid and two from blood cultures) from 43 cases diagnosed between 2003-2007 were analysed. The species were determined through morphological and physiological tests and genotypes were determined by URA5-RFLP and PCR-fingerprinting (wild-type phage M13). The following species and genotypes were identified: Cryptococcus neoformans VNI (28/56, 50%), Cryptococcus gattii VGII (25/56, 44.64%) and C. gattii VGI (3/56, 5.26%). The genotype VNI occurred in 12 out of 14 HIV-positive adults, whereas the genotype VGII occurred in 11 out of 21 HIV-negative adults (p < 0.02, OR = 6.6 IC95% 0.98-56.0). All patients less than 12 years old were HIV negative and six cases were caused by the VGII genotype, one by the VGI and one by VNI. Therefore, endemic primary mycosis in HIV-negative individuals, including an unexpectedly high number of children, caused by the VGII genotype deserves further study and suggests the need for surveillance on cryptococcal infection in the state of Pará, Eastern Amazon.