Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFPA |
Texto Completo: | http://repositorio.ufpa.br/jspui/handle/2011/8397 |
Resumo: | We used biotinylated dextran amine (BDA) to anterogradely label individual axons projecting from primary somatosensory cortex (S1) to four different cortical areas in rats. A major goal was to determine whether axon terminals in these target areas shared morphometric similarities based on the shape of individual terminal arbors and the density of two bouton types: en passant (Bp) and terminaux (Bt). Evidence from tridimensional reconstructions of isolated axon terminal fragments (n=111) did support a degree of morphological heterogeneity establishing two broad groups of axon terminals. Morphological parameters associated with the complexity of terminal arbors and the proportion of beaded Bp vs stalked Bt were found to differ significantly in these two groups following a discriminant function statistical analysis across axon fragments. Interestingly, both groups occurred in all four target areas, possibly consistent with a commonality of presynaptic processing of tactile information. These findings lay the ground for additional work aiming to investigate synaptic function at the single bouton level and see how this might be associated with emerging properties in postsynaptic targets. |
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2017-05-16T12:25:37Z2017-05-16T12:25:37Z2016-05SÁ, Andrea Lima de de et al. Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats. Braziian Journal Medical and Biological Research, Ribeirão Preto, v. 49, n. 6, e5115, 2016. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600601&lng=pt&nrm=iso>. Acesso em: 16 maio 2017. Epub 10-Maio-2016. <http://dx.doi.org/10.1590/1414-431X20155115>.1414-431Xhttp://repositorio.ufpa.br/jspui/handle/2011/8397We used biotinylated dextran amine (BDA) to anterogradely label individual axons projecting from primary somatosensory cortex (S1) to four different cortical areas in rats. A major goal was to determine whether axon terminals in these target areas shared morphometric similarities based on the shape of individual terminal arbors and the density of two bouton types: en passant (Bp) and terminaux (Bt). Evidence from tridimensional reconstructions of isolated axon terminal fragments (n=111) did support a degree of morphological heterogeneity establishing two broad groups of axon terminals. Morphological parameters associated with the complexity of terminal arbors and the proportion of beaded Bp vs stalked Bt were found to differ significantly in these two groups following a discriminant function statistical analysis across axon fragments. Interestingly, both groups occurred in all four target areas, possibly consistent with a commonality of presynaptic processing of tactile information. These findings lay the ground for additional work aiming to investigate synaptic function at the single bouton level and see how this might be associated with emerging properties in postsynaptic targets.porUniversidade Federal do ParáUFPABrasilBrazilian Journal of Medical and Biological ResearchMorfometriaMorfologiaAnálise estatísticaNeurociênciasMorphometric analysis of feedforward pathways from the primary somatosensory area (S1) of ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article496110SÁ, Andrea Lima deBAHIA, Carlomagno PachecoDIAS, Ivanira AmaralBATISTA, C.LEAL, Walace GomesPINHO, André Luís Santos deHOUZEL, Jean ChristopheDINIZ, Cristovam Wanderley PicançoPEREIRA JÚNIOR, AntônioCORREA, Vânia Castroinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPAinstname:Universidade Federal do Pará (UFPA)instacron:UFPATEXTArtigo_MorphometricAnalysisFeedforward.pdf.txtArtigo_MorphometricAnalysisFeedforward.pdf.txtExtracted texttext/plain35049http://repositorio.ufpa.br/oai/bitstream/2011/8397/6/Artigo_MorphometricAnalysisFeedforward.pdf.txte8cad4f4421914ae175819d1c16864c2MD56ORIGINALArtigo_MorphometricAnalysisFeedforward.pdfArtigo_MorphometricAnalysisFeedforward.pdfapplication/pdf3228566http://repositorio.ufpa.br/oai/bitstream/2011/8397/1/Artigo_MorphometricAnalysisFeedforward.pdf656d5f3cbb8b3f4dbd387c8626ebefbaMD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.ufpa.br/oai/bitstream/2011/8397/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://repositorio.ufpa.br/oai/bitstream/2011/8397/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://repositorio.ufpa.br/oai/bitstream/2011/8397/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81866http://repositorio.ufpa.br/oai/bitstream/2011/8397/5/license.txt43cd690d6a359e86c1fe3d5b7cba0c9bMD552011/83972017-10-16 09:27:21.524oai:repositorio.ufpa.br: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ório InstitucionalPUBhttp://repositorio.ufpa.br/oai/requestriufpabc@ufpa.bropendoar:21232017-10-16T12:27:21Repositório Institucional da UFPA - Universidade Federal do Pará (UFPA)false |
dc.title.pt_BR.fl_str_mv |
Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats |
title |
Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats |
spellingShingle |
Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats SÁ, Andrea Lima de Morfometria Morfologia Análise estatística Neurociências |
title_short |
Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats |
title_full |
Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats |
title_fullStr |
Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats |
title_full_unstemmed |
Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats |
title_sort |
Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats |
author |
SÁ, Andrea Lima de |
author_facet |
SÁ, Andrea Lima de BAHIA, Carlomagno Pacheco DIAS, Ivanira Amaral BATISTA, C. LEAL, Walace Gomes PINHO, André Luís Santos de HOUZEL, Jean Christophe DINIZ, Cristovam Wanderley Picanço PEREIRA JÚNIOR, Antônio CORREA, Vânia Castro |
author_role |
author |
author2 |
BAHIA, Carlomagno Pacheco DIAS, Ivanira Amaral BATISTA, C. LEAL, Walace Gomes PINHO, André Luís Santos de HOUZEL, Jean Christophe DINIZ, Cristovam Wanderley Picanço PEREIRA JÚNIOR, Antônio CORREA, Vânia Castro |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
SÁ, Andrea Lima de BAHIA, Carlomagno Pacheco DIAS, Ivanira Amaral BATISTA, C. LEAL, Walace Gomes PINHO, André Luís Santos de HOUZEL, Jean Christophe DINIZ, Cristovam Wanderley Picanço PEREIRA JÚNIOR, Antônio CORREA, Vânia Castro |
dc.subject.por.fl_str_mv |
Morfometria Morfologia Análise estatística Neurociências |
topic |
Morfometria Morfologia Análise estatística Neurociências |
description |
We used biotinylated dextran amine (BDA) to anterogradely label individual axons projecting from primary somatosensory cortex (S1) to four different cortical areas in rats. A major goal was to determine whether axon terminals in these target areas shared morphometric similarities based on the shape of individual terminal arbors and the density of two bouton types: en passant (Bp) and terminaux (Bt). Evidence from tridimensional reconstructions of isolated axon terminal fragments (n=111) did support a degree of morphological heterogeneity establishing two broad groups of axon terminals. Morphological parameters associated with the complexity of terminal arbors and the proportion of beaded Bp vs stalked Bt were found to differ significantly in these two groups following a discriminant function statistical analysis across axon fragments. Interestingly, both groups occurred in all four target areas, possibly consistent with a commonality of presynaptic processing of tactile information. These findings lay the ground for additional work aiming to investigate synaptic function at the single bouton level and see how this might be associated with emerging properties in postsynaptic targets. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-05 |
dc.date.accessioned.fl_str_mv |
2017-05-16T12:25:37Z |
dc.date.available.fl_str_mv |
2017-05-16T12:25:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SÁ, Andrea Lima de de et al. Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats. Braziian Journal Medical and Biological Research, Ribeirão Preto, v. 49, n. 6, e5115, 2016. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600601&lng=pt&nrm=iso>. Acesso em: 16 maio 2017. Epub 10-Maio-2016. <http://dx.doi.org/10.1590/1414-431X20155115>. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpa.br/jspui/handle/2011/8397 |
dc.identifier.issn.pt_BR.fl_str_mv |
1414-431X |
identifier_str_mv |
SÁ, Andrea Lima de de et al. Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats. Braziian Journal Medical and Biological Research, Ribeirão Preto, v. 49, n. 6, e5115, 2016. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600601&lng=pt&nrm=iso>. Acesso em: 16 maio 2017. Epub 10-Maio-2016. <http://dx.doi.org/10.1590/1414-431X20155115>. 1414-431X |
url |
http://repositorio.ufpa.br/jspui/handle/2011/8397 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian Journal of Medical and Biological Research |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal do Pará |
dc.publisher.initials.fl_str_mv |
UFPA |
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Brasil |
publisher.none.fl_str_mv |
Universidade Federal do Pará |
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