Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats

Detalhes bibliográficos
Autor(a) principal: SÁ, Andrea Lima de
Data de Publicação: 2016
Outros Autores: BAHIA, Carlomagno Pacheco, DIAS, Ivanira Amaral, BATISTA, C., LEAL, Walace Gomes, PINHO, André Luís Santos de, HOUZEL, Jean Christophe, DINIZ, Cristovam Wanderley Picanço, PEREIRA JÚNIOR, Antônio, CORREA, Vânia Castro
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFPA
Texto Completo: http://repositorio.ufpa.br/jspui/handle/2011/8397
Resumo: We used biotinylated dextran amine (BDA) to anterogradely label individual axons projecting from primary somatosensory cortex (S1) to four different cortical areas in rats. A major goal was to determine whether axon terminals in these target areas shared morphometric similarities based on the shape of individual terminal arbors and the density of two bouton types: en passant (Bp) and terminaux (Bt). Evidence from tridimensional reconstructions of isolated axon terminal fragments (n=111) did support a degree of morphological heterogeneity establishing two broad groups of axon terminals. Morphological parameters associated with the complexity of terminal arbors and the proportion of beaded Bp vs stalked Bt were found to differ significantly in these two groups following a discriminant function statistical analysis across axon fragments. Interestingly, both groups occurred in all four target areas, possibly consistent with a commonality of presynaptic processing of tactile information. These findings lay the ground for additional work aiming to investigate synaptic function at the single bouton level and see how this might be associated with emerging properties in postsynaptic targets.
id UFPA_f691b69ad8a5fbf303f2441cd2e78d0b
oai_identifier_str oai:repositorio.ufpa.br:2011/8397
network_acronym_str UFPA
network_name_str Repositório Institucional da UFPA
repository_id_str 2123
spelling 2017-05-16T12:25:37Z2017-05-16T12:25:37Z2016-05SÁ, Andrea Lima de de et al. Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats. Braziian Journal Medical and Biological Research, Ribeirão Preto, v. 49, n. 6, e5115, 2016. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600601&lng=pt&nrm=iso>. Acesso em: 16 maio 2017. Epub 10-Maio-2016. <http://dx.doi.org/10.1590/1414-431X20155115>.1414-431Xhttp://repositorio.ufpa.br/jspui/handle/2011/8397We used biotinylated dextran amine (BDA) to anterogradely label individual axons projecting from primary somatosensory cortex (S1) to four different cortical areas in rats. A major goal was to determine whether axon terminals in these target areas shared morphometric similarities based on the shape of individual terminal arbors and the density of two bouton types: en passant (Bp) and terminaux (Bt). Evidence from tridimensional reconstructions of isolated axon terminal fragments (n=111) did support a degree of morphological heterogeneity establishing two broad groups of axon terminals. Morphological parameters associated with the complexity of terminal arbors and the proportion of beaded Bp vs stalked Bt were found to differ significantly in these two groups following a discriminant function statistical analysis across axon fragments. Interestingly, both groups occurred in all four target areas, possibly consistent with a commonality of presynaptic processing of tactile information. These findings lay the ground for additional work aiming to investigate synaptic function at the single bouton level and see how this might be associated with emerging properties in postsynaptic targets.porUniversidade Federal do ParáUFPABrasilBrazilian Journal of Medical and Biological ResearchMorfometriaMorfologiaAnálise estatísticaNeurociênciasMorphometric analysis of feedforward pathways from the primary somatosensory area (S1) of ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article496110SÁ, Andrea Lima deBAHIA, Carlomagno PachecoDIAS, Ivanira AmaralBATISTA, C.LEAL, Walace GomesPINHO, André Luís Santos deHOUZEL, Jean ChristopheDINIZ, Cristovam Wanderley PicançoPEREIRA JÚNIOR, AntônioCORREA, Vânia Castroinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPAinstname:Universidade Federal do Pará (UFPA)instacron:UFPATEXTArtigo_MorphometricAnalysisFeedforward.pdf.txtArtigo_MorphometricAnalysisFeedforward.pdf.txtExtracted texttext/plain35049http://repositorio.ufpa.br/oai/bitstream/2011/8397/6/Artigo_MorphometricAnalysisFeedforward.pdf.txte8cad4f4421914ae175819d1c16864c2MD56ORIGINALArtigo_MorphometricAnalysisFeedforward.pdfArtigo_MorphometricAnalysisFeedforward.pdfapplication/pdf3228566http://repositorio.ufpa.br/oai/bitstream/2011/8397/1/Artigo_MorphometricAnalysisFeedforward.pdf656d5f3cbb8b3f4dbd387c8626ebefbaMD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.ufpa.br/oai/bitstream/2011/8397/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://repositorio.ufpa.br/oai/bitstream/2011/8397/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://repositorio.ufpa.br/oai/bitstream/2011/8397/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81866http://repositorio.ufpa.br/oai/bitstream/2011/8397/5/license.txt43cd690d6a359e86c1fe3d5b7cba0c9bMD552011/83972017-10-16 09:27:21.524oai:repositorio.ufpa.br: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ório InstitucionalPUBhttp://repositorio.ufpa.br/oai/requestriufpabc@ufpa.bropendoar:21232017-10-16T12:27:21Repositório Institucional da UFPA - Universidade Federal do Pará (UFPA)false
dc.title.pt_BR.fl_str_mv Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats
title Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats
spellingShingle Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats
SÁ, Andrea Lima de
Morfometria
Morfologia
Análise estatística
Neurociências
title_short Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats
title_full Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats
title_fullStr Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats
title_full_unstemmed Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats
title_sort Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats
author SÁ, Andrea Lima de
author_facet SÁ, Andrea Lima de
BAHIA, Carlomagno Pacheco
DIAS, Ivanira Amaral
BATISTA, C.
LEAL, Walace Gomes
PINHO, André Luís Santos de
HOUZEL, Jean Christophe
DINIZ, Cristovam Wanderley Picanço
PEREIRA JÚNIOR, Antônio
CORREA, Vânia Castro
author_role author
author2 BAHIA, Carlomagno Pacheco
DIAS, Ivanira Amaral
BATISTA, C.
LEAL, Walace Gomes
PINHO, André Luís Santos de
HOUZEL, Jean Christophe
DINIZ, Cristovam Wanderley Picanço
PEREIRA JÚNIOR, Antônio
CORREA, Vânia Castro
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv SÁ, Andrea Lima de
BAHIA, Carlomagno Pacheco
DIAS, Ivanira Amaral
BATISTA, C.
LEAL, Walace Gomes
PINHO, André Luís Santos de
HOUZEL, Jean Christophe
DINIZ, Cristovam Wanderley Picanço
PEREIRA JÚNIOR, Antônio
CORREA, Vânia Castro
dc.subject.por.fl_str_mv Morfometria
Morfologia
Análise estatística
Neurociências
topic Morfometria
Morfologia
Análise estatística
Neurociências
description We used biotinylated dextran amine (BDA) to anterogradely label individual axons projecting from primary somatosensory cortex (S1) to four different cortical areas in rats. A major goal was to determine whether axon terminals in these target areas shared morphometric similarities based on the shape of individual terminal arbors and the density of two bouton types: en passant (Bp) and terminaux (Bt). Evidence from tridimensional reconstructions of isolated axon terminal fragments (n=111) did support a degree of morphological heterogeneity establishing two broad groups of axon terminals. Morphological parameters associated with the complexity of terminal arbors and the proportion of beaded Bp vs stalked Bt were found to differ significantly in these two groups following a discriminant function statistical analysis across axon fragments. Interestingly, both groups occurred in all four target areas, possibly consistent with a commonality of presynaptic processing of tactile information. These findings lay the ground for additional work aiming to investigate synaptic function at the single bouton level and see how this might be associated with emerging properties in postsynaptic targets.
publishDate 2016
dc.date.issued.fl_str_mv 2016-05
dc.date.accessioned.fl_str_mv 2017-05-16T12:25:37Z
dc.date.available.fl_str_mv 2017-05-16T12:25:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SÁ, Andrea Lima de de et al. Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats. Braziian Journal Medical and Biological Research, Ribeirão Preto, v. 49, n. 6, e5115, 2016. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600601&lng=pt&nrm=iso>. Acesso em: 16 maio 2017. Epub 10-Maio-2016. <http://dx.doi.org/10.1590/1414-431X20155115>.
dc.identifier.uri.fl_str_mv http://repositorio.ufpa.br/jspui/handle/2011/8397
dc.identifier.issn.pt_BR.fl_str_mv 1414-431X
identifier_str_mv SÁ, Andrea Lima de de et al. Morphometric analysis of feedforward pathways from the primary somatosensory area (S1) of rats. Braziian Journal Medical and Biological Research, Ribeirão Preto, v. 49, n. 6, e5115, 2016. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600601&lng=pt&nrm=iso>. Acesso em: 16 maio 2017. Epub 10-Maio-2016. <http://dx.doi.org/10.1590/1414-431X20155115>.
1414-431X
url http://repositorio.ufpa.br/jspui/handle/2011/8397
dc.language.iso.fl_str_mv por
language por
dc.relation.ispartof.pt_BR.fl_str_mv Brazilian Journal of Medical and Biological Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal do Pará
dc.publisher.initials.fl_str_mv UFPA
dc.publisher.country.fl_str_mv Brasil
publisher.none.fl_str_mv Universidade Federal do Pará
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFPA
instname:Universidade Federal do Pará (UFPA)
instacron:UFPA
instname_str Universidade Federal do Pará (UFPA)
instacron_str UFPA
institution UFPA
reponame_str Repositório Institucional da UFPA
collection Repositório Institucional da UFPA
bitstream.url.fl_str_mv http://repositorio.ufpa.br/oai/bitstream/2011/8397/6/Artigo_MorphometricAnalysisFeedforward.pdf.txt
http://repositorio.ufpa.br/oai/bitstream/2011/8397/1/Artigo_MorphometricAnalysisFeedforward.pdf
http://repositorio.ufpa.br/oai/bitstream/2011/8397/2/license_url
http://repositorio.ufpa.br/oai/bitstream/2011/8397/3/license_text
http://repositorio.ufpa.br/oai/bitstream/2011/8397/4/license_rdf
http://repositorio.ufpa.br/oai/bitstream/2011/8397/5/license.txt
bitstream.checksum.fl_str_mv e8cad4f4421914ae175819d1c16864c2
656d5f3cbb8b3f4dbd387c8626ebefba
4afdbb8c545fd630ea7db775da747b2f
d41d8cd98f00b204e9800998ecf8427e
d41d8cd98f00b204e9800998ecf8427e
43cd690d6a359e86c1fe3d5b7cba0c9b
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFPA - Universidade Federal do Pará (UFPA)
repository.mail.fl_str_mv riufpabc@ufpa.br
_version_ 1801771989952626688