Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFPA |
Texto Completo: | http://repositorio.ufpa.br/jspui/handle/2011/3611 |
Resumo: | The present study intended to characterize the phenotypic and genetic diversity of Brazilian isolates of Chromobacterium violaceum from aquatic environments within the Amazon region. Nineteen isolates showed morphological properties of C. violaceum and the majority grew at 44°C. Low temperatures, in contrast, showed to be inhibitory to their growth, as eleven isolates did not grow at 10ºC and nine did not produce pigmentation, clearly indicating an inhibition of their metabolism. The largest variation among isolates was observed in the citrate test (Simmons), in which 12 isolates were positive, and in the oxidation/fermentation of sucrose, with six positives isolates. Chloramphenicol, gentamicin and sulfonamides efficiently inhibited bacterial growth. Amplified products of the recA gene were digested with HindII or PstI, which produced three or four restriction fragments patterns, respectively. The combined analysis arranged the isolates into six genospecies. The higher diversity observed in Belém (genotypes C, D, E and F) may be a consequence of intense human occupation, pollution of the aquatic environment or due to the higher diversity of the environments sampled in that region. In conclusion, a high level of genetic and phenotypic diversity was observed, and four new genospecies were described. |
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2013-04-02T12:58:21Z2013-04-02T12:58:21Z2008-11DALL'AGNOL, L. T., et al. Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 7, p. 678-682, nov. 2008. Disponível em: <http://www.scielo.br/pdf/mioc/v103n7/09.pdf>. Acesso em: 01 abr. 2013. <http://dx.doi.org/10.1590/S0074-02762008000700009>.0074-0276http://repositorio.ufpa.br/jspui/handle/2011/3611The present study intended to characterize the phenotypic and genetic diversity of Brazilian isolates of Chromobacterium violaceum from aquatic environments within the Amazon region. Nineteen isolates showed morphological properties of C. violaceum and the majority grew at 44°C. Low temperatures, in contrast, showed to be inhibitory to their growth, as eleven isolates did not grow at 10ºC and nine did not produce pigmentation, clearly indicating an inhibition of their metabolism. The largest variation among isolates was observed in the citrate test (Simmons), in which 12 isolates were positive, and in the oxidation/fermentation of sucrose, with six positives isolates. Chloramphenicol, gentamicin and sulfonamides efficiently inhibited bacterial growth. Amplified products of the recA gene were digested with HindII or PstI, which produced three or four restriction fragments patterns, respectively. The combined analysis arranged the isolates into six genospecies. The higher diversity observed in Belém (genotypes C, D, E and F) may be a consequence of intense human occupation, pollution of the aquatic environment or due to the higher diversity of the environments sampled in that region. In conclusion, a high level of genetic and phenotypic diversity was observed, and four new genospecies were described.engAmbiente aquáticoChromobacterium violaceumVariação genéticaAmazônia brasileiraDiversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazoninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDALL'AGNOL, Leonardo TeixeiraMARTINS, Rosimar NerisVALLINOTO, Antonio Carlos RosárioRIBEIRO, Karla Tereza Silvainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPAinstname:Universidade Federal do Pará (UFPA)instacron:UFPAORIGINALArtigo_DiversityChromobacteriumViolaceum.pdfArtigo_DiversityChromobacteriumViolaceum.pdfapplication/pdf430703http://repositorio.ufpa.br/oai/bitstream/2011/3611/1/Artigo_DiversityChromobacteriumViolaceum.pdff88557516153472ab419b0ebb534836cMD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.ufpa.br/oai/bitstream/2011/3611/2/license_urlfd26723f8d7edacdb29e3f03465c3b03MD52license_textlicense_texttext/html; charset=utf-80http://repositorio.ufpa.br/oai/bitstream/2011/3611/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-823599http://repositorio.ufpa.br/oai/bitstream/2011/3611/4/license_rdf9e2b7f6edbd693264102b96ece20428aMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81996http://repositorio.ufpa.br/oai/bitstream/2011/3611/5/license.txt4b350777eea7a4b1ae7cd9a06fd687a1MD55TEXTArtigo_DiversityChromobacteriumViolaceum.pdf.txtArtigo_DiversityChromobacteriumViolaceum.pdf.txtExtracted texttext/plain24641http://repositorio.ufpa.br/oai/bitstream/2011/3611/6/Artigo_DiversityChromobacteriumViolaceum.pdf.txte37e0cf40c3cec750f703977862cde60MD562011/36112019-05-23 08:11:45.724oai:repositorio.ufpa.br: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Repositório InstitucionalPUBhttp://repositorio.ufpa.br/oai/requestriufpabc@ufpa.bropendoar:21232019-05-23T11:11:45Repositório Institucional da UFPA - Universidade Federal do Pará (UFPA)false |
dc.title.pt_BR.fl_str_mv |
Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon |
title |
Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon |
spellingShingle |
Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon DALL'AGNOL, Leonardo Teixeira Ambiente aquático Chromobacterium violaceum Variação genética Amazônia brasileira |
title_short |
Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon |
title_full |
Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon |
title_fullStr |
Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon |
title_full_unstemmed |
Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon |
title_sort |
Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon |
author |
DALL'AGNOL, Leonardo Teixeira |
author_facet |
DALL'AGNOL, Leonardo Teixeira MARTINS, Rosimar Neris VALLINOTO, Antonio Carlos Rosário RIBEIRO, Karla Tereza Silva |
author_role |
author |
author2 |
MARTINS, Rosimar Neris VALLINOTO, Antonio Carlos Rosário RIBEIRO, Karla Tereza Silva |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
DALL'AGNOL, Leonardo Teixeira MARTINS, Rosimar Neris VALLINOTO, Antonio Carlos Rosário RIBEIRO, Karla Tereza Silva |
dc.subject.por.fl_str_mv |
Ambiente aquático Chromobacterium violaceum Variação genética Amazônia brasileira |
topic |
Ambiente aquático Chromobacterium violaceum Variação genética Amazônia brasileira |
description |
The present study intended to characterize the phenotypic and genetic diversity of Brazilian isolates of Chromobacterium violaceum from aquatic environments within the Amazon region. Nineteen isolates showed morphological properties of C. violaceum and the majority grew at 44°C. Low temperatures, in contrast, showed to be inhibitory to their growth, as eleven isolates did not grow at 10ºC and nine did not produce pigmentation, clearly indicating an inhibition of their metabolism. The largest variation among isolates was observed in the citrate test (Simmons), in which 12 isolates were positive, and in the oxidation/fermentation of sucrose, with six positives isolates. Chloramphenicol, gentamicin and sulfonamides efficiently inhibited bacterial growth. Amplified products of the recA gene were digested with HindII or PstI, which produced three or four restriction fragments patterns, respectively. The combined analysis arranged the isolates into six genospecies. The higher diversity observed in Belém (genotypes C, D, E and F) may be a consequence of intense human occupation, pollution of the aquatic environment or due to the higher diversity of the environments sampled in that region. In conclusion, a high level of genetic and phenotypic diversity was observed, and four new genospecies were described. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-11 |
dc.date.accessioned.fl_str_mv |
2013-04-02T12:58:21Z |
dc.date.available.fl_str_mv |
2013-04-02T12:58:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
DALL'AGNOL, L. T., et al. Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 7, p. 678-682, nov. 2008. Disponível em: <http://www.scielo.br/pdf/mioc/v103n7/09.pdf>. Acesso em: 01 abr. 2013. <http://dx.doi.org/10.1590/S0074-02762008000700009>. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpa.br/jspui/handle/2011/3611 |
dc.identifier.issn.none.fl_str_mv |
0074-0276 |
identifier_str_mv |
DALL'AGNOL, L. T., et al. Diversity of Chromobacterium violaceum isolates from aquatic environments of state of Pará, Brazilian Amazon. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 7, p. 678-682, nov. 2008. Disponível em: <http://www.scielo.br/pdf/mioc/v103n7/09.pdf>. Acesso em: 01 abr. 2013. <http://dx.doi.org/10.1590/S0074-02762008000700009>. 0074-0276 |
url |
http://repositorio.ufpa.br/jspui/handle/2011/3611 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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UFPA |
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