Transportadores intestinais em codornas japonesas (Coturnix japonica) em diferentes fases

• Autor(a) principal: Silva, Eudes Fernando Alves da
• Data de Publicação: 2019
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFPB
• Texto Completo: https://repositorio.ufpb.br/jspui/handle/123456789/30584
• topic: coturnicultura; qPCR e transportadores de hexoses; CNPQ::CIENCIAS AGRARIAS

The experiment was carried out with the objective of evaluating the gene expression of the sodium and glucose co-transporter type 1 (SGLT1) and glucose transporters type 2 and 5 (GLUT2 and 5) in Japanese quails in different phases. Eightyeight Japanese one-day-old female categories, reared in conditions similar to the creation of a production, with feed and water ad libitum, were used. The design was the casual encounter, were used as different ages (1, 14, 21, 42 and 49 days of life), composed of 3 replicates each. The baseline data of the experimental harvest were as follows: the samples collected and euthanized, then duodenum and jejunum samples were collected for the evaluation of the gene expression of intestinal transporters using a RT-PCR technique. Data were submitted to analysis of variance and as a means of comparison by the Tukey test at 5% probability. Between 1 and 21 days of age, an expression related to SGLT1 reduced at 14 days in both intestines and increased again only at 21 days, with GLUT2 expression in relation to age in both intestinal segments and expression of GLUT5 also increased in relation to an age, just not being jejunum. The increase in transport capacity over one can be a compensatory mechanism with the purpose of increasing the load of its substrates. The highest values of relative expression of SGLT1 were observed at 42 days of duodenum, whereas GLUT5 the highest expression values were observed at 42 days of duodenum and at 49 days in jejunum, these higher values can be recognized at the beginning of the posture phase As a way to ensure maximum absorption of nutrients. Therefore, we conclude that the relative gene expression of the intestinal transporters of SGLT1, GLUT2 and GLUT5 hexoses has been altered in several breeding phases in Japanese quails.