Investigação do mecanismo de ação preventivo da Spirulina platensis nas alterações do acoplamento eletromecânico induzidas pelo treinamento de força em útero de ratas Wistar

• Autor(a) principal: Barros, Bárbara Cavalcanti
• Data de Publicação: 2021
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFPB
• Texto Completo: https://repositorio.ufpb.br/jspui/handle/123456789/22101
• topic: Spirulina platensis; Treinamento de força; Contração uterina; Alterações morfológicas; Óxido nítrico; Fosfolipase A2; Estresse oxidativo; Strength training; Uterine contraction; Morphological changes; Nitric oxide; Phospholipase A2; Oxidative stress; CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA

The PFT is a type of physical exercise, which occurs with a gradual overload for a short period that can generate benefits related to chronic diseases or depending on your prescription and training principles, when inadequately prescribed it can cause losses, leading to to oxidative stress and changes in the female reproductive system. In previous studies, it was observed that PFT increased the efficacy and decreased the contractile potency of KCl and that supplementation with Spirulina platensis (SP) at a dose 100 mg/kg prevented these deleterious effects in the uterus of Wistar rats. Thus, it was decided to investigate the mechanism of action by which SP prevented the changes in electromechanical contractile reactivity induced by TFP in rat uterus. The experimental procedures were approved by CEUA/UFPB (certificate 5191200320). Female Wistar rats were divided into groups: 1) control (CG); 2) trained (TG) and 3) trained and supplemented with SP 100 mg/kg (GTSP100). The PFT was based on the protocol of jumping in a liquid medium with a progressive increase in load. The animals received, 24 hours before euthanasia, diethylstilbestrol (1 mg/kg, s.c.) for estrus induction. After euthanasia, the uterus was isolated, cleaned and suspended in organ bath vats under appropriate experimental conditions. KCl induced isometric contractions were adequately monitored. Thus, histomorphometric parameters of the uterus were evaluated, as well as the participation of the nitric oxide (NO), cyclooxygenase (COX) pathways and the crosstalk between these two pathways, in addition to the oxidative stress/antioxidant defense pathways in the uterine reactivity to KCl. Based on the results obtained, it was observed that the adopted PFT protocol induced histological changes in the uterus, in addition to leading to uterine hypertrophy, and algae supplementation prevents histological damage without altering uterine hypertrophy. In addition, it was observed that TFP increases uterine contractile efficacy due to the reaction of NO with the superoxide anion forming peroxynitrite, increased synthesis of contractile prostanoids, and also an inhibitory crosstalk between the NO and COX pathways and decreased of superoxide dismutase activity. Furthermore, these effects are prevented by supplementation with S. platensis, through activation of the NO pathway, inhibition of the COX pathway, activation of crosstalk between these pathways and inhibition of reactive oxygen species. These data show that in the uterus of female rats there are changes from the TFP prevented by supplementation with SP, so, if these results occur in women, S. platensis would become favorable as a food supplement in the prevention of oxidative damage and uterine contractile dysregulation in women who practice TFP.