Detecção do papilomavírus humano 16 (HPV16) em amostras de carcinoma mamário triplo negativo (TNBC) de mulheres do estado da Paraíba

• Autor(a) principal: Nunes, Zhilbelly da Mota
• Data de Publicação: 2020
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFPB
• Texto Completo: https://repositorio.ufpb.br/jspui/handle/123456789/24512
• topic: Câncer de mama; Papilomavírus humano (HPV); Triplo negativo (TBNC); Papilomavírus humano de alto risco (HR HPV); Breast cancer; Human papillomavirus (HPV); TNBC; qPCR; High-risk human papillomavirus (HR HPV); CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

Breast cancer is a major public health problem worldwide. Several endogenous and exogenous factors are related to breast cancer. Several studies have shown the involvement of Human papillomavirus (HPV) in the cancerous breast, although this relationship is still controversial. Thus, the aims of this study were: 1) detect HPV DNA in paraffinized breast cancer samples Luminal A, Luminal B, HER-2- positive and triple negative (TBNC), from patients in the State of Paraíba, Brazil ; 2) detect HPV16 DNA in TNBC of patients from Paraíba State, Brazil; 3) To perform a an association study between the positive and negative TNBC that were positive to HPV. For this, the detection of viral DNA was performed by real time PCR, using GP5 and GP6 primers set. For genotyping, HPV16 E6 primers were used. In total, 60% (60/100) of tumors were positive to HPV DNA. HPV16 was observed in 100% (60/60) of the tumors positive to HPV. Among TNBC, 67% (10/15) were positive to HPV16. There was no association between HPV and TNBC. The results of this study suggest that viruses are active in tumoral breast cells and play a cancerous role in the breast.