Estudo da atividade do Alcaloide Milonina, em modelos experimentais de inflamação aguda e dor
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFPB |
Texto Completo: | https://repositorio.ufpb.br/jspui/handle/tede/9417 |
Resumo: | Inflammation is an immune response that aims to establish a tissue homeostasis during an infection or injury. Recognized as a beneficial process, an inflammation can become harmful when in excess. Thus, therapeutic therapies that are applied to the resolution of the inflammation are studied and developed, with natural products and their derivatives playing a prominent role in the discoveries of new anti-inflammatory molecules. In this context, the objective of this study was to evaluate the antiinflammatory potential of milonine, a Cissampelos sympodialis Eichl alkaloid, in vivo, against the release of mediators by using the murine model of acute inflammation and pain. Swiss mice pretreated with milonine were submitted to protocols of: paw edema induced by LPS, prostaglandin (PGE2), bradykinin (BK) and serotonin (5-HT) to evaluate the anti-edematogenic activity of the alkaloid; microvascular permeability induced by acetic acid to obtain a total protein concentration and histological analysis of the peritoneum; the models of carrageenan-induced peritonitis to evaluate the effect of milonine on the migration of the total and differential cells; and the formalin test to evaluate its nociceptive activity. The results show that milonine was capable of significantly reduce (p<0.001) the formation of the paw edema induced by LPS, PGE2, BK, however it was not capable to reduce the edema induced by 5-HT; significantly reduced (p<0.05) the extravazation of liquid to the peritoneum induced by acetic acid, maintaining its morphology preserved. The alkaloid was also able to inhibit (p<0.01) the migration of the total leukocytes to the peritoneal cavity during the carrageenan-induced inflammation, decreasing the number of polymorphonuclear cells (PMN) without changing mononuclear cells (MNs). In the formalin test, the paw licking time of the animals was inhibited (p<0.01) only in the second phase through the intraperitoneal (i.p.) administration, corroborating the inhibition of inflammatory mediators. Therefore, this study demonstrates that milonine has an antiinflammatory and analgesic activity due to the inhibition of the action of mediators essential for the start of the inflammatory process. |
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Estudo da atividade do Alcaloide Milonina, em modelos experimentais de inflamação aguda e dorCissampelos sympodialisInflamação agudaAlcaloideMiloninaAnti-inflamatórioNocicepçãoCissampelos sympodialisAcute inflammationAlkaloidMilonineAnti-inflammatoryNociceptionCIENCIAS BIOLOGICAS::BIOLOGIA GERALInflammation is an immune response that aims to establish a tissue homeostasis during an infection or injury. Recognized as a beneficial process, an inflammation can become harmful when in excess. Thus, therapeutic therapies that are applied to the resolution of the inflammation are studied and developed, with natural products and their derivatives playing a prominent role in the discoveries of new anti-inflammatory molecules. In this context, the objective of this study was to evaluate the antiinflammatory potential of milonine, a Cissampelos sympodialis Eichl alkaloid, in vivo, against the release of mediators by using the murine model of acute inflammation and pain. Swiss mice pretreated with milonine were submitted to protocols of: paw edema induced by LPS, prostaglandin (PGE2), bradykinin (BK) and serotonin (5-HT) to evaluate the anti-edematogenic activity of the alkaloid; microvascular permeability induced by acetic acid to obtain a total protein concentration and histological analysis of the peritoneum; the models of carrageenan-induced peritonitis to evaluate the effect of milonine on the migration of the total and differential cells; and the formalin test to evaluate its nociceptive activity. The results show that milonine was capable of significantly reduce (p<0.001) the formation of the paw edema induced by LPS, PGE2, BK, however it was not capable to reduce the edema induced by 5-HT; significantly reduced (p<0.05) the extravazation of liquid to the peritoneum induced by acetic acid, maintaining its morphology preserved. The alkaloid was also able to inhibit (p<0.01) the migration of the total leukocytes to the peritoneal cavity during the carrageenan-induced inflammation, decreasing the number of polymorphonuclear cells (PMN) without changing mononuclear cells (MNs). In the formalin test, the paw licking time of the animals was inhibited (p<0.01) only in the second phase through the intraperitoneal (i.p.) administration, corroborating the inhibition of inflammatory mediators. Therefore, this study demonstrates that milonine has an antiinflammatory and analgesic activity due to the inhibition of the action of mediators essential for the start of the inflammatory process.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESO processo inflamatório é uma resposta benéfica do organismo frente a uma lesão ou infecção, visando a eliminação da injúria inicial, bem como as consequências dessa injúria. Entretanto, a inflamação, em resposta exagerada, pode ser prejudicial. Dessa forma, se faz necessário o desenvolvimento de novos fármacos com propriedades anti-inflamatória e analgésica, sendo utilizado como estratégia terapêutica a busca por moléculas oriundas de produtos naturais que possuam tais propriedades. Nesse contexto, o objetivo desse estudo foi avaliar o potencial anti-inflamatório da milonina, alcaloide de Cissampelos sympodialis Eichl frente à liberação de mediadores inflamatórios utilizando o modelo murino de inflamação aguda e dor. Camundongos Swiss pré-tratados com milonina foram submetidos aos protocolos de: edema de pata induzido por LPS, prostaglandina (PGE2), bradicinina (BK) e serotonina (5-HT) para avaliar a atividade antiedematogênica do alcaloide; permeabilidade microvascular induzida por ácido acético para obter a concentração de proteínas totais e análise histológica do peritônio; os modelos de peritonite induzida por carragenina para avaliar o efeito da milonina sobre a migração de células totais e diferenciais; e o teste da formalina para avaliar a sua atividade nociceptiva. Os resultados demonstraram que a milonina foi capaz de reduzir significativamente (p<0,001) a formação do edema de pata induzido por LPS, PGE2, BK, porém não foi capaz de reduzir o edema induzido pela 5-HT; diminuiu significativamente (p<0,05) o extravasamento de líquido para o peritônio induzido por ácido acético, mantendo a sua morfologia preservada. O alcaloide também foi capaz de inibir (p<0,01) a migração de leucócitos totais para a cavidade peritoneal durante a inflamação induzida por carragenina, diminuindo o número de células polimorfonucleares (PMN) sem alterar células mononucleares (MNs). No teste da formalina, o tempo de lambida da pata dos animais foi inibida (p<0,01) por milonina apenas na segunda fase quando administrada por via intraperitoneal (i.p.). Portanto, este estudo demonstrou que a milonina possui atividade anti-inflamatória e analgésica por inibir a ação de mediadores essenciais para o início do processo inflamatório.Universidade Federal da ParaíbaBrasilBiologia Celular e MolecularPrograma de Pós-Graduação em Biologia Celular e MolecularUFPBPiuvezam, Marcia Reginahttp://lattes.cnpq.br/0961955935523938Silva, Larissa Rodrigues2017-09-05T12:23:50Z2018-07-20T23:36:15Z2018-07-20T23:36:15Z2017-04-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfSILVA, Larissa Rodrigues. Estudo da atividade do Alcaloide Milonina, em modelos experimentais de inflamação aguda e dor. 2017. 87 f. Dissertação (Mestrado em Biologia Celular e Molecular) - Universidade Federal da Paraíba, João Pessoa, 2017.https://repositorio.ufpb.br/jspui/handle/tede/9417porinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFPBinstname:Universidade Federal da Paraíba (UFPB)instacron:UFPB2018-09-05T23:56:22Zoai:repositorio.ufpb.br:tede/9417Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufpb.br/PUBhttp://tede.biblioteca.ufpb.br:8080/oai/requestdiretoria@ufpb.br|| diretoria@ufpb.bropendoar:2018-09-05T23:56:22Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB)false |
dc.title.none.fl_str_mv |
Estudo da atividade do Alcaloide Milonina, em modelos experimentais de inflamação aguda e dor |
title |
Estudo da atividade do Alcaloide Milonina, em modelos experimentais de inflamação aguda e dor |
spellingShingle |
Estudo da atividade do Alcaloide Milonina, em modelos experimentais de inflamação aguda e dor Silva, Larissa Rodrigues Cissampelos sympodialis Inflamação aguda Alcaloide Milonina Anti-inflamatório Nocicepção Cissampelos sympodialis Acute inflammation Alkaloid Milonine Anti-inflammatory Nociception CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
title_short |
Estudo da atividade do Alcaloide Milonina, em modelos experimentais de inflamação aguda e dor |
title_full |
Estudo da atividade do Alcaloide Milonina, em modelos experimentais de inflamação aguda e dor |
title_fullStr |
Estudo da atividade do Alcaloide Milonina, em modelos experimentais de inflamação aguda e dor |
title_full_unstemmed |
Estudo da atividade do Alcaloide Milonina, em modelos experimentais de inflamação aguda e dor |
title_sort |
Estudo da atividade do Alcaloide Milonina, em modelos experimentais de inflamação aguda e dor |
author |
Silva, Larissa Rodrigues |
author_facet |
Silva, Larissa Rodrigues |
author_role |
author |
dc.contributor.none.fl_str_mv |
Piuvezam, Marcia Regina http://lattes.cnpq.br/0961955935523938 |
dc.contributor.author.fl_str_mv |
Silva, Larissa Rodrigues |
dc.subject.por.fl_str_mv |
Cissampelos sympodialis Inflamação aguda Alcaloide Milonina Anti-inflamatório Nocicepção Cissampelos sympodialis Acute inflammation Alkaloid Milonine Anti-inflammatory Nociception CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
topic |
Cissampelos sympodialis Inflamação aguda Alcaloide Milonina Anti-inflamatório Nocicepção Cissampelos sympodialis Acute inflammation Alkaloid Milonine Anti-inflammatory Nociception CIENCIAS BIOLOGICAS::BIOLOGIA GERAL |
description |
Inflammation is an immune response that aims to establish a tissue homeostasis during an infection or injury. Recognized as a beneficial process, an inflammation can become harmful when in excess. Thus, therapeutic therapies that are applied to the resolution of the inflammation are studied and developed, with natural products and their derivatives playing a prominent role in the discoveries of new anti-inflammatory molecules. In this context, the objective of this study was to evaluate the antiinflammatory potential of milonine, a Cissampelos sympodialis Eichl alkaloid, in vivo, against the release of mediators by using the murine model of acute inflammation and pain. Swiss mice pretreated with milonine were submitted to protocols of: paw edema induced by LPS, prostaglandin (PGE2), bradykinin (BK) and serotonin (5-HT) to evaluate the anti-edematogenic activity of the alkaloid; microvascular permeability induced by acetic acid to obtain a total protein concentration and histological analysis of the peritoneum; the models of carrageenan-induced peritonitis to evaluate the effect of milonine on the migration of the total and differential cells; and the formalin test to evaluate its nociceptive activity. The results show that milonine was capable of significantly reduce (p<0.001) the formation of the paw edema induced by LPS, PGE2, BK, however it was not capable to reduce the edema induced by 5-HT; significantly reduced (p<0.05) the extravazation of liquid to the peritoneum induced by acetic acid, maintaining its morphology preserved. The alkaloid was also able to inhibit (p<0.01) the migration of the total leukocytes to the peritoneal cavity during the carrageenan-induced inflammation, decreasing the number of polymorphonuclear cells (PMN) without changing mononuclear cells (MNs). In the formalin test, the paw licking time of the animals was inhibited (p<0.01) only in the second phase through the intraperitoneal (i.p.) administration, corroborating the inhibition of inflammatory mediators. Therefore, this study demonstrates that milonine has an antiinflammatory and analgesic activity due to the inhibition of the action of mediators essential for the start of the inflammatory process. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09-05T12:23:50Z 2017-04-28 2018-07-20T23:36:15Z 2018-07-20T23:36:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SILVA, Larissa Rodrigues. Estudo da atividade do Alcaloide Milonina, em modelos experimentais de inflamação aguda e dor. 2017. 87 f. Dissertação (Mestrado em Biologia Celular e Molecular) - Universidade Federal da Paraíba, João Pessoa, 2017. https://repositorio.ufpb.br/jspui/handle/tede/9417 |
identifier_str_mv |
SILVA, Larissa Rodrigues. Estudo da atividade do Alcaloide Milonina, em modelos experimentais de inflamação aguda e dor. 2017. 87 f. Dissertação (Mestrado em Biologia Celular e Molecular) - Universidade Federal da Paraíba, João Pessoa, 2017. |
url |
https://repositorio.ufpb.br/jspui/handle/tede/9417 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal da Paraíba Brasil Biologia Celular e Molecular Programa de Pós-Graduação em Biologia Celular e Molecular UFPB |
publisher.none.fl_str_mv |
Universidade Federal da Paraíba Brasil Biologia Celular e Molecular Programa de Pós-Graduação em Biologia Celular e Molecular UFPB |
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reponame:Biblioteca Digital de Teses e Dissertações da UFPB instname:Universidade Federal da Paraíba (UFPB) instacron:UFPB |
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Universidade Federal da Paraíba (UFPB) |
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UFPB |
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UFPB |
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Biblioteca Digital de Teses e Dissertações da UFPB |
collection |
Biblioteca Digital de Teses e Dissertações da UFPB |
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Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB) |
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diretoria@ufpb.br|| diretoria@ufpb.br |
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1801842862512406528 |