Desenvolvimento e validação de uma metodologia analítica para determinação de resíduos de agrotóxicos em hortaliças empregando cromatografia líquida com detector de arranjo de diodos

• Autor(a) principal: Sousa, Emanuella Santos
• Data de Publicação: 2016
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFPB
• Texto Completo: https://repositorio.ufpb.br/jspui/handle/tede/9215
• topic: Hortaliças; Pesticidas; Cromatografia em fase líquida; QuEChERS; Validação; Vegetables; Pesticides; Liquid chromatography; QuEChERS; Validation; CIENCIAS EXATAS E DA TERRA::QUIMICA

The use of pesticide is increasing worldwide especially in Brazil, with a consumption of approximately 5.2 kg/inhabitant. For this reason, national and international regulatory agencies (Codex, FAO and ANVISA) monitor and establish assessments criteria for the quality control of food, regulating the maximum residue limits (MRLs), restricting and prohibiting the use of these products that causes harmful effects to health and the environment. The majority of the methods uses liquid or gas chromatography with mass detector, fluorescence and/or diode array for quantification of pesticides in foods. This paper proposes a methodology for quantifying seven pesticides (carbendazim, thiabendazole, fuberidazole, carbofuran, flutriafol, carbaryl and its degradation product 1-naphthol) in vegetables using QuEChERS extraction, without chean up step, and liquid chromatography with diode array detection. In the chromatographic procedure it was employed a gradient elution of 0.014mM phosphoric acid (eluent A) and methanol (eluent B): 40% B for 3.5 minutes reaching a ratio of 55% B at 22 minutes with a flow rate of 1 mL.min-1 and a temperature of 35°C. These chromatographic parameters and calibration models were developed, optimized and validated aiming an analysis free of interference and to achieve the MRLs established by the national and international standard-setting organizations (INMETRO, ANVISA, EUROCHEM, FAO and Codex). It was found 0.21 mg kg-1 carbendazim and 0.31 mg kg-1 carbofuran in cabbage sample; 0.12 mg kg-1 in beet flutriafol; 0.05 mg kg-1, fuberidazole and flutriafol in tomato and 0.24 mg kg-1 carbendazim in green paprika. It is important to highlight, that ANVISA allows only the use of flutriafol in tomato, with maximum permitted limit of 0.1 mg kg-1 and for the other samples there is no authorization for the use of these pesticides to combat pests on vegetables.