Efeito inibitório de micropartículas de vidro bioativo dopado com prata sobre formas promastigotas de Leishmania amazonensis e Leishmania braziliensis

Detalhes bibliográficos
Autor(a) principal: Pires, Emanuene Galdino
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFPB
Texto Completo: https://repositorio.ufpb.br/jspui/handle/123456789/11642
Resumo: Leishmaniasis is a parasitic infectious disease caused by several species of protozoa of the genus Leishmania affecting humans and different animal species, inducing mucocutaneous lesions. The purpose of this study was to evaluate the inhibitory effect of silver doped bioactive glass microparticles over promastigote forms of Leishmania amazonensis (L.Amazonensis) and Leishmania braziliensis (L. braziliensis). Microparticles of bioactive glass (BG) and doped with silver (BGAg1 and BGAg2) belonging to the system 58SiO2·(36-x)CaO·6P2O5·xAg2O with x = 0, 1 and 2 mol%, respectively were synthesized via sol–gel method and characterized by Scanning Electron Microscopy (SEM), Thermal Gravimetric Analysis (TGA), XRay Diffraction (XRD) and Fourier Transform Infrared Espectroscopy (FTIR). The cytotoxity of the BG and BGAg in human cells was assessed from the response of A549 lung adenocarcinoma ephitelial cells line. L.Amazonensis and L. braziliensis promastigote cultures were exposed to BG, BGAg1 and BGAg2 for 24h. Then, 0.5 mM resazurin was added to culture and absorbance readings were assessed after 48, 72 and 96 hours of incubation. The percentage of resazurin reduction per group was obtained from a calculator (AbDSerotec®) and viable promastigote forms were counted on Neubauer chamber. SEM images showed micrometric particles with irregular and porous surface. TGA analysis demonstrated, for samples doped with silver, an endotermic peak around 497ºC associated to silver oxide decomposition. The XRD patterns exhibit mainly amorphous characteristics corresponding to BG. FTIR curves revealed main vibrational modes including Si-O-Si asymmetric stretching vibration (1000-1200 cm-1), Si-O-Si symmetric stretching vibration (750800 cm-1), Si-O-Si bending mode (450-480 cm-1) and PO4-3 antisymmetric bending (570-600 cm-1). Reffering to cytotoxicity, BGAg showed non-toxic behavior. BGAg1- 300µg/mL inihibited L. amazonensis and BGAg2 300 µg/mL inihibited L. braziliensis with 97,6 % and 92% of efficacy, respectively. The count on Neubauer chamber confirmed the efficacy of BGAg2 in 150 e 300 µg/mL concentrations revealing absence of viable cells. In conclusion, the BGAg2- 150 and 300 µg/mL inhibited the growth and proliferation of L.amazonensis and L. braziliensis on promastigote form and could be used in other studies, like in vivo investigations that are necessary to verify the BGAg ativity on Leishmania amastigotes forms.
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spelling Efeito inibitório de micropartículas de vidro bioativo dopado com prata sobre formas promastigotas de Leishmania amazonensis e Leishmania braziliensisLeishmaniosePrataVidro BioativoSíntese Sol-gelLeishmaniasisSilverBioactive glassSol-gel synthesisCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIALeishmaniasis is a parasitic infectious disease caused by several species of protozoa of the genus Leishmania affecting humans and different animal species, inducing mucocutaneous lesions. The purpose of this study was to evaluate the inhibitory effect of silver doped bioactive glass microparticles over promastigote forms of Leishmania amazonensis (L.Amazonensis) and Leishmania braziliensis (L. braziliensis). Microparticles of bioactive glass (BG) and doped with silver (BGAg1 and BGAg2) belonging to the system 58SiO2·(36-x)CaO·6P2O5·xAg2O with x = 0, 1 and 2 mol%, respectively were synthesized via sol–gel method and characterized by Scanning Electron Microscopy (SEM), Thermal Gravimetric Analysis (TGA), XRay Diffraction (XRD) and Fourier Transform Infrared Espectroscopy (FTIR). The cytotoxity of the BG and BGAg in human cells was assessed from the response of A549 lung adenocarcinoma ephitelial cells line. L.Amazonensis and L. braziliensis promastigote cultures were exposed to BG, BGAg1 and BGAg2 for 24h. Then, 0.5 mM resazurin was added to culture and absorbance readings were assessed after 48, 72 and 96 hours of incubation. The percentage of resazurin reduction per group was obtained from a calculator (AbDSerotec®) and viable promastigote forms were counted on Neubauer chamber. SEM images showed micrometric particles with irregular and porous surface. TGA analysis demonstrated, for samples doped with silver, an endotermic peak around 497ºC associated to silver oxide decomposition. The XRD patterns exhibit mainly amorphous characteristics corresponding to BG. FTIR curves revealed main vibrational modes including Si-O-Si asymmetric stretching vibration (1000-1200 cm-1), Si-O-Si symmetric stretching vibration (750800 cm-1), Si-O-Si bending mode (450-480 cm-1) and PO4-3 antisymmetric bending (570-600 cm-1). Reffering to cytotoxicity, BGAg showed non-toxic behavior. BGAg1- 300µg/mL inihibited L. amazonensis and BGAg2 300 µg/mL inihibited L. braziliensis with 97,6 % and 92% of efficacy, respectively. The count on Neubauer chamber confirmed the efficacy of BGAg2 in 150 e 300 µg/mL concentrations revealing absence of viable cells. In conclusion, the BGAg2- 150 and 300 µg/mL inhibited the growth and proliferation of L.amazonensis and L. braziliensis on promastigote form and could be used in other studies, like in vivo investigations that are necessary to verify the BGAg ativity on Leishmania amastigotes forms.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESA leishmaniose é uma doença parasitária infecciosa causada por várias espécies de protozoários do gênero Leishmania que acomete o homem e diferentes espécies de animais, induzindo lesões mucocutâneas. O objetivo deste estudo foi avaliar o efeito inibitório de micropartículas de vidro bioativo dopado com prata sobre formas promastigotas de Leishmania amazonenses (L.Amazonensis) e Leishmania braziliensis (L. braziliensis). Micropartículas de vidro bioativo puro (Bioactive Glass- BG) e dopado com prata (BGAg1 e BGAg2) pertencentes ao sistema 58SiO2·(36x)CaO·6P2O5·xAg2O com x = 0, 1 e 2 mol%, respectivamente, foram sintetizadas através do método sol-gel e caracterizadas por Microscopia Eletrônica de Varredura (SEM), Análise Termogravimétrica (TGA), Difratometria de Raios-X (XRD) e Espectroscopia de Adsorção no Infra-Vermelho com Transformada de Fourier (FTIR). A citotoxicidade do BG e BGAg em células humanas foi avaliada a partir da resposta de células de adenocarcinoma pulmonar da linhagem A549. As culturas de formas promastigotas de L. Amazonensis e L. braziliensis foram expostas às amostras de BG, BGAg1 e BGAg2 por 24h. Em seguida, 0,5 mM de resazurina foi adicionada à cultura e leituras de absorbância foram realizadas após 48, 72 e 96h de incubação. O percentual de redução da resazurina por grupo foi obtido a partir de uma calculadora (ABD Serotec®) e as formas promastigotas viáveis foram contadas em câmara de Neubauer. As imagens da SEM mostraram partículas micrométricas com superfície irregular e porosa. A TGA demonstrou, para as amostras com prata, um pico endotérmico em torno de 497ºC associado à decomposição do óxido de prata. Os padrões de XRD exibiram características essencialmente amorfas correspondentes ao BG. As curvas de FTIR revelaram os principais modos vibracionais, incluindo: vibração assimétrica de alongamento do Si-O-Si (1000-1200 cm-1), vibração simétrica de alongamento do Si-O-Si (750-800 cm-1), modo de flexão do Si-O-Si (450-480 cm-1) e flexão assimétrica do PO4-3(570600 cm-1). No que se refere à citotoxicidade, o BGAg mostrou comportamento não tóxico. O BGAg1- 300µg/mL inibiu L. amazonensis e o BGAg2- 300µg/mL inibiu L. braziliensis com 97,6% e 92% de eficácia, respectivamente. A contagem em câmara de Neubauer confirmou a eficácia do BGAg2 nas concentrações de 150 e 300µg/mL revelando a ausência de células viáveis. Em conclusão, o BGAg2- 150 e 300 µg/mL inibiu o crescimento e proliferação de L.amazonensis e L. braziliensis na forma promastigota e poderia ser utilizado em outros estudos, como investigações in vivo, que são necessários para verificar a atividade do BGAg nas formas amastigotas de Leishmania.Universidade Federal da ParaíbaBrasilOdontologiaPrograma de Pós-Graduação em OdontologiaUFPBBonan, Paulo Rogério Ferretihttp://lattes.cnpq.br/4201967424037508Castellano, Lúcio Roberto CançadoPires, Emanuene Galdino2018-09-10T17:16:25Z2018-09-102018-09-10T17:16:25Z2015-12-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttps://repositorio.ufpb.br/jspui/handle/123456789/11642porinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFPBinstname:Universidade Federal da Paraíba (UFPB)instacron:UFPB2018-09-11T06:02:30Zoai:repositorio.ufpb.br:123456789/11642Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufpb.br/PUBhttp://tede.biblioteca.ufpb.br:8080/oai/requestdiretoria@ufpb.br|| diretoria@ufpb.bropendoar:2018-09-11T06:02:30Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB)false
dc.title.none.fl_str_mv Efeito inibitório de micropartículas de vidro bioativo dopado com prata sobre formas promastigotas de Leishmania amazonensis e Leishmania braziliensis
title Efeito inibitório de micropartículas de vidro bioativo dopado com prata sobre formas promastigotas de Leishmania amazonensis e Leishmania braziliensis
spellingShingle Efeito inibitório de micropartículas de vidro bioativo dopado com prata sobre formas promastigotas de Leishmania amazonensis e Leishmania braziliensis
Pires, Emanuene Galdino
Leishmaniose
Prata
Vidro Bioativo
Síntese Sol-gel
Leishmaniasis
Silver
Bioactive glass
Sol-gel synthesis
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
title_short Efeito inibitório de micropartículas de vidro bioativo dopado com prata sobre formas promastigotas de Leishmania amazonensis e Leishmania braziliensis
title_full Efeito inibitório de micropartículas de vidro bioativo dopado com prata sobre formas promastigotas de Leishmania amazonensis e Leishmania braziliensis
title_fullStr Efeito inibitório de micropartículas de vidro bioativo dopado com prata sobre formas promastigotas de Leishmania amazonensis e Leishmania braziliensis
title_full_unstemmed Efeito inibitório de micropartículas de vidro bioativo dopado com prata sobre formas promastigotas de Leishmania amazonensis e Leishmania braziliensis
title_sort Efeito inibitório de micropartículas de vidro bioativo dopado com prata sobre formas promastigotas de Leishmania amazonensis e Leishmania braziliensis
author Pires, Emanuene Galdino
author_facet Pires, Emanuene Galdino
author_role author
dc.contributor.none.fl_str_mv Bonan, Paulo Rogério Ferreti
http://lattes.cnpq.br/4201967424037508
Castellano, Lúcio Roberto Cançado
dc.contributor.author.fl_str_mv Pires, Emanuene Galdino
dc.subject.por.fl_str_mv Leishmaniose
Prata
Vidro Bioativo
Síntese Sol-gel
Leishmaniasis
Silver
Bioactive glass
Sol-gel synthesis
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
topic Leishmaniose
Prata
Vidro Bioativo
Síntese Sol-gel
Leishmaniasis
Silver
Bioactive glass
Sol-gel synthesis
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
description Leishmaniasis is a parasitic infectious disease caused by several species of protozoa of the genus Leishmania affecting humans and different animal species, inducing mucocutaneous lesions. The purpose of this study was to evaluate the inhibitory effect of silver doped bioactive glass microparticles over promastigote forms of Leishmania amazonensis (L.Amazonensis) and Leishmania braziliensis (L. braziliensis). Microparticles of bioactive glass (BG) and doped with silver (BGAg1 and BGAg2) belonging to the system 58SiO2·(36-x)CaO·6P2O5·xAg2O with x = 0, 1 and 2 mol%, respectively were synthesized via sol–gel method and characterized by Scanning Electron Microscopy (SEM), Thermal Gravimetric Analysis (TGA), XRay Diffraction (XRD) and Fourier Transform Infrared Espectroscopy (FTIR). The cytotoxity of the BG and BGAg in human cells was assessed from the response of A549 lung adenocarcinoma ephitelial cells line. L.Amazonensis and L. braziliensis promastigote cultures were exposed to BG, BGAg1 and BGAg2 for 24h. Then, 0.5 mM resazurin was added to culture and absorbance readings were assessed after 48, 72 and 96 hours of incubation. The percentage of resazurin reduction per group was obtained from a calculator (AbDSerotec®) and viable promastigote forms were counted on Neubauer chamber. SEM images showed micrometric particles with irregular and porous surface. TGA analysis demonstrated, for samples doped with silver, an endotermic peak around 497ºC associated to silver oxide decomposition. The XRD patterns exhibit mainly amorphous characteristics corresponding to BG. FTIR curves revealed main vibrational modes including Si-O-Si asymmetric stretching vibration (1000-1200 cm-1), Si-O-Si symmetric stretching vibration (750800 cm-1), Si-O-Si bending mode (450-480 cm-1) and PO4-3 antisymmetric bending (570-600 cm-1). Reffering to cytotoxicity, BGAg showed non-toxic behavior. BGAg1- 300µg/mL inihibited L. amazonensis and BGAg2 300 µg/mL inihibited L. braziliensis with 97,6 % and 92% of efficacy, respectively. The count on Neubauer chamber confirmed the efficacy of BGAg2 in 150 e 300 µg/mL concentrations revealing absence of viable cells. In conclusion, the BGAg2- 150 and 300 µg/mL inhibited the growth and proliferation of L.amazonensis and L. braziliensis on promastigote form and could be used in other studies, like in vivo investigations that are necessary to verify the BGAg ativity on Leishmania amastigotes forms.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-15
2018-09-10T17:16:25Z
2018-09-10
2018-09-10T17:16:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.ufpb.br/jspui/handle/123456789/11642
url https://repositorio.ufpb.br/jspui/handle/123456789/11642
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal da Paraíba
Brasil
Odontologia
Programa de Pós-Graduação em Odontologia
UFPB
publisher.none.fl_str_mv Universidade Federal da Paraíba
Brasil
Odontologia
Programa de Pós-Graduação em Odontologia
UFPB
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFPB
instname:Universidade Federal da Paraíba (UFPB)
instacron:UFPB
instname_str Universidade Federal da Paraíba (UFPB)
instacron_str UFPB
institution UFPB
reponame_str Biblioteca Digital de Teses e Dissertações da UFPB
collection Biblioteca Digital de Teses e Dissertações da UFPB
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB)
repository.mail.fl_str_mv diretoria@ufpb.br|| diretoria@ufpb.br
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