Aplicação da espectrometria de massas para o estudo químico da fração acetato do vinho tinto Syrah do Vale do São Francisco e estudo da sua atividade antioxidante e anti-hipertensiva
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFPB |
Texto Completo: | https://repositorio.ufpb.br/jspui/handle/123456789/15028 |
Resumo: | Wine has high levels of polyphenols which include both flavonoids and non-flavonoids. Epidemiological studies have demonstrated an association between the consumption of phenolics rich food and beverages and reduce risk of cardiovascular diseases. Hypertension is a major risk factor for cardiovascular disease. Among the factors associated with the development of hypertension, an extremely important and current issue is the participation of reactive oxygen species (ROS) in its pathogenesis. Thus, the aim of this study was to investigate the antioxidant and anti-hypertensive effect of a rich phenolic fraction derived from red wine (Syrah from the São Francisco Valley region), in spontaneously hypertensive rats. The wine was partitioned with EtOAc at different pHs yielding three fractions. The total phenolic content of wine and fractions was determined by the Folin-Ciocalteu method and expressed as milligrams of gallic acid equivalent per liter or per extract gram of extract (mg GAE/L; mg GAE/ g). The antiradicalar activity was determined using the DPPH (with ascorbic acid as positive control), the Trolox Equivalent Antioxidant Capacity (TEAC) or the ABTS•+ test (with Trolox as a positive control). The determination of monomeric anthocyanins was carried out by the differential pH method. The chromatographic profile and the determination and quantification of quercetin and trans-resveratrol was performed by HPLC-UV. Ultra High Performance Liquid Chromatography coupled with Electrospray Ionization High resolution Time of Flight Mass Spectrometry (UPLC-ESI-TOF) was used to identify the compounds present in pH 7 acetate fraction (FrAcOEt SyRe VSF). Spontaneously hypertensive rats (SHR) were divided into 4 groups (n=32): SHR + saline (n = 8); SHR + FrAcOEt pH 7 Syre ESV (50 mg/kg) (n = 8); SHR + saline (n = 8); SHR + FrAcOEt pH 7 Syre ESV (100 mg/kg) (n = 8) and treated with the respective daily doses V.O. by gavage for two weeks. 24h after cannulation blood pressure, heart rate were recorded and baroreflex stimulated. Oxidative stress was measured via lipid peroxidation (TBARS). Superior mesenteric artery rings isolated from normotensive rats were used to evaluate the vasorelaxant effect of FrAcOEt pH 7 SyRe VSF. The FrAcOEt pH 7 SyRe VSF had the largest total phenolic content (58.45 ± 0.01 mg GAE/g) and monomeric anthocyanins (4.99 ± 0.019 mg/L) compared to other fractions. In addition it showed the best antioxidant activity against the DPPH and ABTS radical. The quercetin content and transresveratrol in FrAcOEt pH 7 SyRe VSF were respectively: 8.56 ± 0.078 and 1.11 ± 0.009 mg/mL. The UPLC/MS analysis of FrAcOEt pH 7 SyRe VSF allowed the identification of 13 compounds, including phenolic acids and flavonoids of various classes. Treatment of animals with FrAcOEt pH 7 SyRe VSF at 50 mg/kg and 100 mg/kg significantly reduced blood pressure when compared to the control group (146.1 ± 4.062 n = 7 vs 159.0 ± 3.891 n = 7, respectively) and (126.5 ± 5.322 n = 8 3.039 ± 150.0 vs. n = 7, respectively). Only at 100 mg/kg the fraction was able to significantly decrease the heart rate compared to the control group (314.6 ± 9.507 n = 8, 358.6 ± 15.00 n = 7, respectively) and to decrease lipid peroxidation (0.9250 nmol/L ± 0.1750 n = 4 vs. 2.180 nmol/L ± 0.3891 n = 5, respectively. There was no improvement in baroreflex sensitivity in neither dose. The cumulative addition of FrAcOEt pH 7 SyRe VSF caused vasorelaxation independent on the presence of vascular endothelium, with Emax = 103.7 ± 9.9% n = 7 in the presence of endothelium and Emax = 105.6 ± 5.9% n = 6 in the absence of endothelium. Thus, such results highlight the efficacy of the extraction process and suggest that FrAcOEt pH 7 SyRe VSF induces an in vivo antihypertensive effect and that this effect may be related to the content of phenolic compounds present in the fraction, even though probably some polyphenols present in wine and important for the biological effects observed here were concentrated in the other fractions not evaluated in this study. In addition, the in vitro activity observed for FrAcOEt pH 7 SyRe VSF, inducing vasorelaxant effect independent of vascular endothelium, probably involves calcium channel blockade and potassium channel opening. |
id |
UFPB_53649cf75d8725f4dc33c7267e365e58 |
---|---|
oai_identifier_str |
oai:repositorio.ufpb.br:123456789/15028 |
network_acronym_str |
UFPB |
network_name_str |
Biblioteca Digital de Teses e Dissertações da UFPB |
repository_id_str |
|
spelling |
Aplicação da espectrometria de massas para o estudo químico da fração acetato do vinho tinto Syrah do Vale do São Francisco e estudo da sua atividade antioxidante e anti-hipertensivaVinhoFenólicosHipertensãoWinePhenolicsHypertensionCNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIAWine has high levels of polyphenols which include both flavonoids and non-flavonoids. Epidemiological studies have demonstrated an association between the consumption of phenolics rich food and beverages and reduce risk of cardiovascular diseases. Hypertension is a major risk factor for cardiovascular disease. Among the factors associated with the development of hypertension, an extremely important and current issue is the participation of reactive oxygen species (ROS) in its pathogenesis. Thus, the aim of this study was to investigate the antioxidant and anti-hypertensive effect of a rich phenolic fraction derived from red wine (Syrah from the São Francisco Valley region), in spontaneously hypertensive rats. The wine was partitioned with EtOAc at different pHs yielding three fractions. The total phenolic content of wine and fractions was determined by the Folin-Ciocalteu method and expressed as milligrams of gallic acid equivalent per liter or per extract gram of extract (mg GAE/L; mg GAE/ g). The antiradicalar activity was determined using the DPPH (with ascorbic acid as positive control), the Trolox Equivalent Antioxidant Capacity (TEAC) or the ABTS•+ test (with Trolox as a positive control). The determination of monomeric anthocyanins was carried out by the differential pH method. The chromatographic profile and the determination and quantification of quercetin and trans-resveratrol was performed by HPLC-UV. Ultra High Performance Liquid Chromatography coupled with Electrospray Ionization High resolution Time of Flight Mass Spectrometry (UPLC-ESI-TOF) was used to identify the compounds present in pH 7 acetate fraction (FrAcOEt SyRe VSF). Spontaneously hypertensive rats (SHR) were divided into 4 groups (n=32): SHR + saline (n = 8); SHR + FrAcOEt pH 7 Syre ESV (50 mg/kg) (n = 8); SHR + saline (n = 8); SHR + FrAcOEt pH 7 Syre ESV (100 mg/kg) (n = 8) and treated with the respective daily doses V.O. by gavage for two weeks. 24h after cannulation blood pressure, heart rate were recorded and baroreflex stimulated. Oxidative stress was measured via lipid peroxidation (TBARS). Superior mesenteric artery rings isolated from normotensive rats were used to evaluate the vasorelaxant effect of FrAcOEt pH 7 SyRe VSF. The FrAcOEt pH 7 SyRe VSF had the largest total phenolic content (58.45 ± 0.01 mg GAE/g) and monomeric anthocyanins (4.99 ± 0.019 mg/L) compared to other fractions. In addition it showed the best antioxidant activity against the DPPH and ABTS radical. The quercetin content and transresveratrol in FrAcOEt pH 7 SyRe VSF were respectively: 8.56 ± 0.078 and 1.11 ± 0.009 mg/mL. The UPLC/MS analysis of FrAcOEt pH 7 SyRe VSF allowed the identification of 13 compounds, including phenolic acids and flavonoids of various classes. Treatment of animals with FrAcOEt pH 7 SyRe VSF at 50 mg/kg and 100 mg/kg significantly reduced blood pressure when compared to the control group (146.1 ± 4.062 n = 7 vs 159.0 ± 3.891 n = 7, respectively) and (126.5 ± 5.322 n = 8 3.039 ± 150.0 vs. n = 7, respectively). Only at 100 mg/kg the fraction was able to significantly decrease the heart rate compared to the control group (314.6 ± 9.507 n = 8, 358.6 ± 15.00 n = 7, respectively) and to decrease lipid peroxidation (0.9250 nmol/L ± 0.1750 n = 4 vs. 2.180 nmol/L ± 0.3891 n = 5, respectively. There was no improvement in baroreflex sensitivity in neither dose. The cumulative addition of FrAcOEt pH 7 SyRe VSF caused vasorelaxation independent on the presence of vascular endothelium, with Emax = 103.7 ± 9.9% n = 7 in the presence of endothelium and Emax = 105.6 ± 5.9% n = 6 in the absence of endothelium. Thus, such results highlight the efficacy of the extraction process and suggest that FrAcOEt pH 7 SyRe VSF induces an in vivo antihypertensive effect and that this effect may be related to the content of phenolic compounds present in the fraction, even though probably some polyphenols present in wine and important for the biological effects observed here were concentrated in the other fractions not evaluated in this study. In addition, the in vitro activity observed for FrAcOEt pH 7 SyRe VSF, inducing vasorelaxant effect independent of vascular endothelium, probably involves calcium channel blockade and potassium channel opening.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqO vinho, bebida obtida a partir do processo de fermentação da uva, apresenta altos níveis de polifenóis que incluem os flavonoides, e os não flavonoides. Estudos epidemiológicos têm demonstrado a associação entre o consumo de alimentos e bebidas ricos em compostos fenólicos e a redução do risco de doenças cardiovasculares. A hipertensão é o maior fator de risco para doenças cardiovasculares. Dentre os fatores associados ao desenvolvimento da hipertensão, uma questão extremamente importante e atual é a participação de espécies reativas de oxigênio (ERO) em sua patogênese. Desse modo, o objetivo desse trabalho foi investigar o efeito antioxidante e anti-hipertensivo de uma fração oriunda de vinho tinto Syrah da região do Vale do São Francisco, com composição química definida e elevado teor de polifenóis, em ratos espontaneamente hipertensos. O vinho foi particionado com AcOEt em diferentes pHs obtendo-se três frações. O teor de fenólicos totais no vinho e frações foi determinado pelo método de Folin-Ciocalteu e expresso como miligramas de equivalente de ácido gálico por litro ou grama de extrato (mg EAG/L; mg EAG/g). A atividade antiradicalar foi avaliada utilizando o radical DPPH (com ácido ascórbico como controle positivo) e Capacidade Antioxidante Equivalente ao Trolox (CAET) ou teste do ABTS·+ (com Trolox como controle positivo). Utilizou-se o método do pH diferencial para determinação de antocianinas monoméricas. A análise do perfil cromatográfico e quantificação de quercetina e transresveratrol foi realizada através de CLAE-UV e UPLC-MS/MS foi utilizada para identificação dos compostos presentes na fração acetato pH 7 (FrAcOEt SyVSF). Ratos espontaneamente hipertensos (SHR) (n=32) foram divididos em 4 grupos: SHR+ salina (n=8); SHR + FrAcOEt pH 7 SyRe VSF (50 mg/Kg) (n=8); SHR+ salina (n=8); SHR + FrAcOEt pH 7 SyRe VSF (100 mg/Kg) (n=8) e tratados com as respectivas doses diárias v.o. por gavagem, durante quinze dias. Um dia após a canulação a pressão sanguínea e a frequência cardíaca foram registradas e o barorreflexo estimulado. O estresse oxidativo foi mensurado via peroxidação lipídica (TBARS). Foram realizados experimentos em aneis de artéria mesentérica superior isolada de rato normotenso para avaliar o efeito vasorelaxante da FrAcOEt pH 7 SyRe VSF. A FrAcOEt pH 7 SyRe VSF revelou o maior teor de fenólicos totais (58,45±0,01 mg EAG/g) e antocianinas monoméricas (4,99±0,019 mg/L) em relação a outras frações, além disso apresentou a melhor atividade antioxidante frente aos radicais DPPH e ABTS. Os teores de quercetina e transresveratrol na FrAcOEt pH 7 SyRe VSF foram respectivamente: 8,56±0,078 e 1,11±0,009 µg/mL. A análise por UPLC/MS da FrAcOEt pH 7 SyRe VSF permitiu a identificação de 13 compostos, entre eles ácidos fenólicos e flavonoides de diversas classes. O tratamento dos animais com a FrAcOEt pH 7 SyRe VSF em ambas as doses 50 mg/Kg e 100 mg/Kg reduziu significativamente a pressão arterial quando comparado ao grupo controle (146,1 ± 4.062 n=7 vs 159,0 ± 3,891 n=7, respectivamente) e (126,5 ± 5,322 n=8 vs 150.0 ± 3.039 n=7, respectivamente) para as doses de 50 e 100mg/Kg respectivamente. Apenas a dose de 100 mg/Kg foi capaz de diminuir significativamente a frequência cardíaca comparando com o grupo controle (314,6 ± 9,507 n=8 vs 358,6 ± 15,00 n=7, respectivamente) e a peroxidação lipídica (0,9250 nmol/L ± 0,1750 n=4 vs 2,180 nmol/L ± 0,3891 n=5, respectivamente. Não foi observado melhora na sensibilidade do barorreflexo em nenhuma das doses. A adição cumulativa da FrAcOEt pH 7 SyRe VSF foi capaz de induzir vasorelaxamento independente de endotélio, uma vez que apresentou Emax= 103,7 ± 9,9 % n=7 na presença do endotélio e Emax= 105,6 ± 5,9% n=6 na ausência do mesmo. Desse modo, tais resultados ressaltam a eficácia do processo de extração e sugerem que a FrAcOEt pH 7 SyRe VSF induz um efeito anti-hipertensivo in vivo e que este efeito pode estar relacionado com o conteúdo de compostos fenólicos presentes na fração; mesmo que, provavelmente alguns polifenois presentes no vinho e importantes para o efeitos biológicos verificados tenham ficado concentrados nas outras frações não avaliadas neste estudo. Além disso a atividade in vitro observada para a FrAcOEt pH 7 SyRe VSF, induzindo efeito vasorelaxante independente do endotélio vascular, provavelmente envolve o bloqueio de canais para cálcio e abertura de canais para potássio.Universidade Federal da ParaíbaBrasilFarmacologiaPrograma de Pós-Graduação em Produtos Naturais e Sintéticos BioativosUFPBOliveira, Eduardo de Jesushttp://lattes.cnpq.br/1293090609428232Braga, Valdir de AndradeFigueiredo, Eugênia Abrantes de2019-07-10T16:32:25Z2019-07-102019-07-10T16:32:25Z2017-02-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesishttps://repositorio.ufpb.br/jspui/handle/123456789/15028porAttribution-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nd/3.0/br/info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFPBinstname:Universidade Federal da Paraíba (UFPB)instacron:UFPB2019-07-10T16:32:25Zoai:repositorio.ufpb.br:123456789/15028Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufpb.br/PUBhttp://tede.biblioteca.ufpb.br:8080/oai/requestdiretoria@ufpb.br|| diretoria@ufpb.bropendoar:2019-07-10T16:32:25Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB)false |
dc.title.none.fl_str_mv |
Aplicação da espectrometria de massas para o estudo químico da fração acetato do vinho tinto Syrah do Vale do São Francisco e estudo da sua atividade antioxidante e anti-hipertensiva |
title |
Aplicação da espectrometria de massas para o estudo químico da fração acetato do vinho tinto Syrah do Vale do São Francisco e estudo da sua atividade antioxidante e anti-hipertensiva |
spellingShingle |
Aplicação da espectrometria de massas para o estudo químico da fração acetato do vinho tinto Syrah do Vale do São Francisco e estudo da sua atividade antioxidante e anti-hipertensiva Figueiredo, Eugênia Abrantes de Vinho Fenólicos Hipertensão Wine Phenolics Hypertension CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
title_short |
Aplicação da espectrometria de massas para o estudo químico da fração acetato do vinho tinto Syrah do Vale do São Francisco e estudo da sua atividade antioxidante e anti-hipertensiva |
title_full |
Aplicação da espectrometria de massas para o estudo químico da fração acetato do vinho tinto Syrah do Vale do São Francisco e estudo da sua atividade antioxidante e anti-hipertensiva |
title_fullStr |
Aplicação da espectrometria de massas para o estudo químico da fração acetato do vinho tinto Syrah do Vale do São Francisco e estudo da sua atividade antioxidante e anti-hipertensiva |
title_full_unstemmed |
Aplicação da espectrometria de massas para o estudo químico da fração acetato do vinho tinto Syrah do Vale do São Francisco e estudo da sua atividade antioxidante e anti-hipertensiva |
title_sort |
Aplicação da espectrometria de massas para o estudo químico da fração acetato do vinho tinto Syrah do Vale do São Francisco e estudo da sua atividade antioxidante e anti-hipertensiva |
author |
Figueiredo, Eugênia Abrantes de |
author_facet |
Figueiredo, Eugênia Abrantes de |
author_role |
author |
dc.contributor.none.fl_str_mv |
Oliveira, Eduardo de Jesus http://lattes.cnpq.br/1293090609428232 Braga, Valdir de Andrade |
dc.contributor.author.fl_str_mv |
Figueiredo, Eugênia Abrantes de |
dc.subject.por.fl_str_mv |
Vinho Fenólicos Hipertensão Wine Phenolics Hypertension CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
topic |
Vinho Fenólicos Hipertensão Wine Phenolics Hypertension CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
description |
Wine has high levels of polyphenols which include both flavonoids and non-flavonoids. Epidemiological studies have demonstrated an association between the consumption of phenolics rich food and beverages and reduce risk of cardiovascular diseases. Hypertension is a major risk factor for cardiovascular disease. Among the factors associated with the development of hypertension, an extremely important and current issue is the participation of reactive oxygen species (ROS) in its pathogenesis. Thus, the aim of this study was to investigate the antioxidant and anti-hypertensive effect of a rich phenolic fraction derived from red wine (Syrah from the São Francisco Valley region), in spontaneously hypertensive rats. The wine was partitioned with EtOAc at different pHs yielding three fractions. The total phenolic content of wine and fractions was determined by the Folin-Ciocalteu method and expressed as milligrams of gallic acid equivalent per liter or per extract gram of extract (mg GAE/L; mg GAE/ g). The antiradicalar activity was determined using the DPPH (with ascorbic acid as positive control), the Trolox Equivalent Antioxidant Capacity (TEAC) or the ABTS•+ test (with Trolox as a positive control). The determination of monomeric anthocyanins was carried out by the differential pH method. The chromatographic profile and the determination and quantification of quercetin and trans-resveratrol was performed by HPLC-UV. Ultra High Performance Liquid Chromatography coupled with Electrospray Ionization High resolution Time of Flight Mass Spectrometry (UPLC-ESI-TOF) was used to identify the compounds present in pH 7 acetate fraction (FrAcOEt SyRe VSF). Spontaneously hypertensive rats (SHR) were divided into 4 groups (n=32): SHR + saline (n = 8); SHR + FrAcOEt pH 7 Syre ESV (50 mg/kg) (n = 8); SHR + saline (n = 8); SHR + FrAcOEt pH 7 Syre ESV (100 mg/kg) (n = 8) and treated with the respective daily doses V.O. by gavage for two weeks. 24h after cannulation blood pressure, heart rate were recorded and baroreflex stimulated. Oxidative stress was measured via lipid peroxidation (TBARS). Superior mesenteric artery rings isolated from normotensive rats were used to evaluate the vasorelaxant effect of FrAcOEt pH 7 SyRe VSF. The FrAcOEt pH 7 SyRe VSF had the largest total phenolic content (58.45 ± 0.01 mg GAE/g) and monomeric anthocyanins (4.99 ± 0.019 mg/L) compared to other fractions. In addition it showed the best antioxidant activity against the DPPH and ABTS radical. The quercetin content and transresveratrol in FrAcOEt pH 7 SyRe VSF were respectively: 8.56 ± 0.078 and 1.11 ± 0.009 mg/mL. The UPLC/MS analysis of FrAcOEt pH 7 SyRe VSF allowed the identification of 13 compounds, including phenolic acids and flavonoids of various classes. Treatment of animals with FrAcOEt pH 7 SyRe VSF at 50 mg/kg and 100 mg/kg significantly reduced blood pressure when compared to the control group (146.1 ± 4.062 n = 7 vs 159.0 ± 3.891 n = 7, respectively) and (126.5 ± 5.322 n = 8 3.039 ± 150.0 vs. n = 7, respectively). Only at 100 mg/kg the fraction was able to significantly decrease the heart rate compared to the control group (314.6 ± 9.507 n = 8, 358.6 ± 15.00 n = 7, respectively) and to decrease lipid peroxidation (0.9250 nmol/L ± 0.1750 n = 4 vs. 2.180 nmol/L ± 0.3891 n = 5, respectively. There was no improvement in baroreflex sensitivity in neither dose. The cumulative addition of FrAcOEt pH 7 SyRe VSF caused vasorelaxation independent on the presence of vascular endothelium, with Emax = 103.7 ± 9.9% n = 7 in the presence of endothelium and Emax = 105.6 ± 5.9% n = 6 in the absence of endothelium. Thus, such results highlight the efficacy of the extraction process and suggest that FrAcOEt pH 7 SyRe VSF induces an in vivo antihypertensive effect and that this effect may be related to the content of phenolic compounds present in the fraction, even though probably some polyphenols present in wine and important for the biological effects observed here were concentrated in the other fractions not evaluated in this study. In addition, the in vitro activity observed for FrAcOEt pH 7 SyRe VSF, inducing vasorelaxant effect independent of vascular endothelium, probably involves calcium channel blockade and potassium channel opening. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-02-20 2019-07-10T16:32:25Z 2019-07-10 2019-07-10T16:32:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufpb.br/jspui/handle/123456789/15028 |
url |
https://repositorio.ufpb.br/jspui/handle/123456789/15028 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal da Paraíba Brasil Farmacologia Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos UFPB |
publisher.none.fl_str_mv |
Universidade Federal da Paraíba Brasil Farmacologia Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos UFPB |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UFPB instname:Universidade Federal da Paraíba (UFPB) instacron:UFPB |
instname_str |
Universidade Federal da Paraíba (UFPB) |
instacron_str |
UFPB |
institution |
UFPB |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UFPB |
collection |
Biblioteca Digital de Teses e Dissertações da UFPB |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB) |
repository.mail.fl_str_mv |
diretoria@ufpb.br|| diretoria@ufpb.br |
_version_ |
1801842950658850816 |